LOCUS       NM_001039051            5387 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus protein kinase inhibitor beta, cAMP dependent, testis
            specific (Pkib), transcript variant 3, mRNA.
ACCESSION   NM_001039051
VERSION     NM_001039051.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Koldaeva,A., Zhang,C., Huang,Y.P., Reinert,J.K., Mizuno,S.,
            Sugiyama,F., Takahashi,S., Soliman,T., Matsunami,H. and Fukunaga,I.
  TITLE     Generation and Characterization of a Cell Type-Specific, Inducible
            Cre-Driver Line to Study Olfactory Processing
  JOURNAL   J Neurosci 41 (30), 6449-6467 (2021)
   PUBMED   34099512
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Blanchet,E., Van de Velde,S., Matsumura,S., Hao,E., LeLay,J.,
            Kaestner,K. and Montminy,M.
  TITLE     Feedback inhibition of CREB signaling promotes beta cell
            dysfunction in insulin resistance
  JOURNAL   Cell Rep 10 (7), 1149-1157 (2015)
   PUBMED   25704817
REFERENCE   3  (bases 1 to 5387)
  AUTHORS   Piazzon,N., Maisonneuve,C., Guilleret,I., Rotman,S. and
            Constam,D.B.
  TITLE     Bicc1 links the regulation of cAMP signaling in polycystic kidneys
            to microRNA-induced gene silencing
  JOURNAL   J Mol Cell Biol 4 (6), 398-408 (2012)
   PUBMED   22641646
REFERENCE   4  (bases 1 to 5387)
  AUTHORS   Belyamani,M., Gangolli,E.A. and Idzerda,R.L.
  TITLE     Reproductive function in protein kinase inhibitor-deficient mice
  JOURNAL   Mol Cell Biol 21 (12), 3959-3963 (2001)
   PUBMED   11359903
REFERENCE   5  (bases 1 to 5387)
  AUTHORS   Scarpetta,M.A., Uhler,M.D. and Meisler,M.H.
  TITLE     The mouse Prkacn2 gene encoding protein kinase A inhibitor 2 is
            located on proximal chromosome 10
  JOURNAL   Mamm Genome 7 (12), 921-922 (1996)
   PUBMED   8995769
REFERENCE   6  (bases 1 to 5387)
  AUTHORS   Seasholtz,A.F., Gamm,D.M., Ballestero,R.P., Scarpetta,M.A. and
            Uhler,M.D.
  TITLE     Differential expression of mRNAs for protein kinase inhibitor
            isoforms in mouse brain
  JOURNAL   Proc Natl Acad Sci U S A 92 (5), 1734-1738 (1995)
   PUBMED   7878050
REFERENCE   7  (bases 1 to 5387)
  AUTHORS   Wen,W., Taylor,S.S. and Meinkoth,J.L.
  TITLE     The expression and intracellular distribution of the heat-stable
            protein kinase inhibitor is cell cycle regulated
  JOURNAL   J Biol Chem 270 (5), 2041-2046 (1995)
   PUBMED   7836431
REFERENCE   8  (bases 1 to 5387)
  AUTHORS   Baude,E.J., Dignam,S.S., Olsen,S.R., Reimann,E.M. and Uhler,M.D.
  TITLE     Glutamic acid 203 of the cAMP-dependent protein kinase catalytic
            subunit participates in the inhibition by two isoforms of the
            protein kinase inhibitor
  JOURNAL   J Biol Chem 269 (3), 2316-2323 (1994)
   PUBMED   7905001
REFERENCE   9  (bases 1 to 5387)
  AUTHORS   Scarpetta,M.A. and Uhler,M.D.
  TITLE     Evidence for two additional isoforms of the endogenous protein
            kinase inhibitor of cAMP-dependent protein kinase in mouse
  JOURNAL   J Biol Chem 268 (15), 10927-10931 (1993)
   PUBMED   7684369
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102647.8, AK005281.1 and AC168055.3.
            
            On Jun 19, 2010 this sequence version replaced NM_001039051.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream start codon, compared to variant 1. The encoded isoform
            (2) is shorter than isoform 1. Variants 2 through 6 encode the same
            isoform (2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK156950.1, AK005281.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                AC102647.8         176521-176590
            71-1356             AK005281.1         3-1288
            1357-5387           AC168055.3         122345-126375
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 29.22 cM"
     gene            1..5387
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /note="protein kinase inhibitor beta, cAMP dependent,
                     testis specific"
                     /db_xref="GeneID:18768"
                     /db_xref="MGI:MGI:101937"
     exon            1..227
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /inference="alignment:Splign:2.1.0"
     exon            228..319
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /inference="alignment:Splign:2.1.0"
     exon            320..387
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /inference="alignment:Splign:2.1.0"
     exon            388..564
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /inference="alignment:Splign:2.1.0"
     CDS             396..632
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /note="isoform 2 is encoded by transcript variant 3;
                     cAMP-dependent protein kinase inhibitor beta; PKI-beta;
                     protein kinase, cAMP-dependent, catalytic, inhibitor 2"
                     /codon_start=1
                     /product="cAMP-dependent protein kinase inhibitor beta
                     isoform 2"
                     /protein_id="NP_001034140.1"
                     /db_xref="CCDS:CCDS23855.1"
                     /db_xref="GeneID:18768"
                     /db_xref="MGI:MGI:101937"
                     /translation="MRTDSSEMTDVESVITSFASSARAGRRNALPDIQSSLATSGSSD
                     LPLKLEALAVKEDAKTKNEEKDQGQPKTPLNEGK"
     exon            565..5387
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      618..623
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /note="hexamer: AATGAA"
     regulatory      628..633
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /note="hexamer: AATGAA"
     polyA_site      646
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /note="major polyA site"
     polyA_site      650
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
     polyA_site      693
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
     regulatory      1335..1340
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /note="hexamer: AATAAA"
     polyA_site      1358
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
     regulatory      3183..3188
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
                     /note="hexamer: AATAAA"
     polyA_site      3203
                     /gene="Pkib"
                     /gene_synonym="Pkibeta; Prkacn2"
ORIGIN      
        1 gcttaactgg gcaggtcctg tggagcctag ctcagctcgc gggggcggga ccgagcggct
       61 ccctccgccc cacacattgc tgccaccagc tcagcccagc cgcatcccag agcagaggcg
      121 gctgcaagct ccagccgcgt cgcagctccg tgccgcagag aggccggcag caggcgccac
      181 cgtgagggac ctggcccggc gccgcccgca tctcttgcgc gcagctgggg tcatgatata
      241 ctggaaacca cacaagatta gtctgtggag gcagaaacca taaagcccct gaaaccaggt
      301 tcggagagga acgataaagg aaaaactccc tttcggcttc atccttcacc tgtcttagaa
      361 acaacagcac attttaacca cagatttatt ctgtgatgag gacagattca tcagagatga
      421 ctgatgtgga atctgtgatc accagctttg cgtcctcagc aagggcaggc cgccgcaatg
      481 ccttacccga catccagagt tcactggcta caagtggatc ctctgatctt ccactgaaac
      541 tggaagcgtt ggccgtgaag gaagatgcaa aaacgaagaa tgaagagaaa gaccaaggcc
      601 aaccaaaaac acccctaaat gaaggaaaat gaaggttcag aattcatcaa gggtgttgat
      661 tttatgctca ttgaaagaca tagcagctat gtattcctgg ggtgtttgat ctggtagtaa
      721 tcatggtaac ccatggtagc gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
      781 gtgtgtgtgt gtgtgcaagc gcgcgcgagc gtgtgtttta ggtaattttg ttgtgatgct
      841 ctgtccttaa actgcagcca tgatacggaa ataggttatt gaaaggtgtt actttcaaat
      901 tgcacccaag gaaggacaca ctgtttatgc cccaacaaaa tccactgtct atcgtgggat
      961 ttacttagtc agatatatgc ttaattctgt cttagtgagt tggaacattt ttcttggtat
     1021 gtatagaccg aaatctatta atttaataca gtttagaatt tgtaggaaat tatatttttt
     1081 tggtgagatg gaattagcag taaaaaaatc acacttataa aatgtgttta tttttaatta
     1141 aaattttcac catatttcct gtaaaatatt aggagagatc tgaaagcagc gtaaacattg
     1201 taacaactta aacatctcta ggatgttttt gtctaatgta ttttccatct agtctgaatg
     1261 tactgtaaat tttcatatgg acccacataa cataaaattt gtgacaaact tgcttatata
     1321 aaacatgtgt attgaataaa tctcactata ttccttaact tggtgtatgt gtgcaccttg
     1381 tttaatgtgg gttgtacaag atattgtgct ccttttctta gactgttttt ttattctctg
     1441 gacttttgtg tgtatatgca aattgttgct agaataattt catcagaatg ctgatcttaa
     1501 tttttatatt ttttctaaag tataatacta gaagttaaaa gagtaaaata catgctttaa
     1561 atatatattt aaatataggt tttttccttt tttattgaaa atagattttc atctcataca
     1621 ttacatccca accacaattt tctctccttc cattccccct atttcttccc cacagatgct
     1681 ttcccctaga tccactctca ttttcccttc agaaaagagc aggcctccaa gaagcaacag
     1741 gaaacacaca aaaaagaaga tacattaaga caatgcaaaa gccctccaat ccaatctgca
     1801 cagggcaacc caggaggatg aaaagcgtcc caagagtagg caaaagaatc agagagtcgc
     1861 ccactcccat tgtttggagt ttcacaaaaa cataaagcta aaagccacaa catatatgca
     1921 gaagacctgc tgcagacccc tgcaggcccc gtgcttgtcc cttcagtgag ctcctgtgaa
     1981 ccctgcttag ctgactctgt aggccatgtt ctcctggtgt cttccatcct ccctgtcgtc
     2041 tacaatcttt cctccctgac ttccacagag tagttttctt tttaaaagag aacagaaaca
     2101 ttaactgaat agaatcccac agtagaaatt catcactgat acaaatttcc tttattttgt
     2161 cttctgaaat aggctgggta taaagggagg atacaattga aagggtggag gatggtcttg
     2221 taccttaaat cccctgctgc ttgtgcctgt atggataaag gttaactttg tgactatgga
     2281 taccagaggc agttttccaa gagcttcatt gttttcagct ctcagatata tatatatatg
     2341 agcattagta tggaggtgtg catttaatta atatttcata aaatatatta gtcattaatt
     2401 tttaaaaatt ttttatgaac attgtataat ggagtgatac tgaatgattg acagaatgat
     2461 tgactgaatg attgacagag agaacagttg ttcccaaata ggctgggatt atgattcagc
     2521 ctcctggaga actttgacac agtctggaga ccactgggga aaggaagtgg gggcagaagt
     2581 tgctgctgga atggagagaa cagagctggg atgctgctat taacgggtag tagaccttat
     2641 gaaggtctcc acagcaggat tgtatagcct ttgctgtcag tattactgaa gtggagagac
     2701 cggagaaaga actccctgga tttgaatttt attcctatgt actagcgatg tgatcttgaa
     2761 tgaagaagat cctatctgtt aaatttccac cataccctgt gtggaatgga actgataaca
     2821 attttcattt tatagtgagt gtgatggttt gtttttatca gcaacttgac acgcctagga
     2881 tcacctgggg gaggaagagt ctcaatgagg aattttctag attagcttac ctctgggaga
     2941 ttgtcttgat tacactaatc catgtgggaa gacccagtgt gctgtgggtg gtactattca
     3001 ttaggtttgg gctcctagat ttcataagag tgggggaaaa aatgggcaga gtatctataa
     3061 ggcatgcctg cattgatttc ctctctgctc tggactgtgg atgtgatggg taaggttgtc
     3121 ttgagctcct ggtactgtgc cttcctctca gagatggact gtaacctgga gttgttacct
     3181 acaataaacc ctgtctctcc caagttgctt ttgaagggta ttttatcaca acagcatcga
     3241 taaactaagg cagtgggtgt agccagtaat gatactgtat acgtaaagca cttgaataat
     3301 gcccagaaga aatgttttga taaacattgg ctattatcat caccatgctc cacggcactt
     3361 ggctttattt gttggtcagg aggtaggttg gtagctaaat tggttagttg actctaattt
     3421 tacaaatgtc cctaagtcat agtgttggga gaatagattc tattggaaat ggactcttgg
     3481 aagtatttac cacatctgta aaaaattcag tcatgttaat aagtgtctga caggtaatga
     3541 gtgtaaatgt atcttgtatt tgcctgatat aatagtcatc tccttgactt actgcctctg
     3601 cctgctaact aaggcctacc agtgatgtag ttcattctat gagagcaaac ttcagggacc
     3661 tccagcttga catccatgtc attaatgaca gttaatcttc actatcaact taggtggatc
     3721 tagaattacc caggagatat acctctgaac atatatggga gagtagctca gagaggctga
     3781 tctcaatgag gcttatcctg aagatgggca gcatcccttc acaggctccc agactgaata
     3841 aaaagtaaag tggaaaaagt cagccgacca ccaatgttca tttctctctg actcctgact
     3901 gcaaagacaa tgggagcagg tacttctttt taacattcta ggtttttttc ttccagtctg
     3961 gcactcaaaa ctctccctcc gcaaagctgg cttaaagcag ctttatgtgt gtctgtgaag
     4021 cacataacca ggtttatcac agtagctctg ttcttcatag cagtattcta aagtttcttg
     4081 ttgctgtgac aaaatacctt agacaaccaa atgaaggcgg aaggatatcc tttggctcac
     4141 tgtttgaaag gatgtacagt ggaacatagc aggaagacag gaagaatgat ggggtgcact
     4201 gctctcttat gtagcttccc cttcctgtct catctcgtaa gagtttgggc atttcttgtt
     4261 ctgtcaaatg ttcctctgat tcatcacttt gtctgccact caattagaca tcactttcaa
     4321 atataggcac tttcttctac aaactaagtt taccttcatt tttgggatta aagatgtgta
     4381 ctaaggatat gtctatattc cagccagaga gattaaaggt atgtacaaag ggctgagcca
     4441 tactacaaaa acaaaaacaa caacaacaaa aacaacaaca acaacaaaaa accaggggtt
     4501 ttgagaaaat aacacaatct cagggtttac aatgtaatca aatatctgac aacagaagag
     4561 ccttgctttt tttgtggatg ccagtagagt tttatgatag ttgtttaaca aaataattgt
     4621 gagtcagtat ttaaatgagt aagttttttt ctatgaaaat aaagaaaaat aagcaacaca
     4681 tagctaataa tggcattttc tttcatttac acccaaactg tctttcgtca ttattactag
     4741 cagtgataaa tattctgtat tatgtatctc ctacatcaca atatatcata gtgtatacac
     4801 atatacaatg ccccattaca tgtgtgcata ttgtatatta tatgtacata ttatatatat
     4861 atatatatat aatatggaca tttgccttat aaagatatca gactcattga caaagaatat
     4921 ttagagaaaa agaatatatt tattatttca ctaggaggca aagacaaagg aaaatttgag
     4981 gctagaagtt attttaaaac aaaattcaaa tttctcatga gcattttgtt ttctttttag
     5041 tgtaaagagt actatatttt aggaagtaga cactctcagt ggaaattgct tgactgggca
     5101 ggtgtagcta ataaacaaaa gacttcagct catttctgta ttgtctttgt gtacggctcc
     5161 tacttgagtt aattattaga cagttttaat taacccggtc atacaatgag gctaaaatga
     5221 aacaataaag ctattgagtg gctgtgcaca aatttacctc tctccaaatg aagagagaga
     5281 acaaacataa tccattctgt gtcctgtgct ggggatgctt ttgtgatata agggaggggg
     5341 atgttgagtc ttatcgggat gtgactaaga gtcgagcatc ctactgc
//
