LOCUS       NM_001410525            3955 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus protein kinase inhibitor, alpha (Pkia), transcript
            variant 6, mRNA.
ACCESSION   NM_001410525
VERSION     NM_001410525.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3955)
  AUTHORS   Hoy,J.J., Salinas Parra,N., Park,J., Kuhn,S. and
            Iglesias-Bartolome,R.
  TITLE     Protein kinase A inhibitor proteins (PKIs) divert GPCR-Galphas-cAMP
            signaling toward EPAC and ERK activation and are involved in tumor
            growth
  JOURNAL   FASEB J 34 (10), 13900-13917 (2020)
   PUBMED   32830375
  REMARK    GeneRIF: Protein kinase A inhibitor proteins (PKIs) divert
            GPCR-Galphas-cAMP signaling toward EPAC and ERK activation and are
            involved in tumor growth.
REFERENCE   4  (bases 1 to 3955)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643. PMID: 29144450]
REFERENCE   5  (bases 1 to 3955)
  AUTHORS   Kim,J., Li,G., Walters,M.A., Taylor,S.S. and Veglia,G.
  TITLE     Uncoupling Catalytic and Binding Functions in the Cyclic
            AMP-Dependent Protein Kinase A
  JOURNAL   Structure 24 (3), 353-363 (2016)
   PUBMED   26833386
REFERENCE   6  (bases 1 to 3955)
  AUTHORS   Seasholtz,A.F., Gamm,D.M., Ballestero,R.P., Scarpetta,M.A. and
            Uhler,M.D.
  TITLE     Differential expression of mRNAs for protein kinase inhibitor
            isoforms in mouse brain
  JOURNAL   Proc Natl Acad Sci U S A 92 (5), 1734-1738 (1995)
   PUBMED   7878050
REFERENCE   7  (bases 1 to 3955)
  AUTHORS   Wen,W., Taylor,S.S. and Meinkoth,J.L.
  TITLE     The expression and intracellular distribution of the heat-stable
            protein kinase inhibitor is cell cycle regulated
  JOURNAL   J Biol Chem 270 (5), 2041-2046 (1995)
   PUBMED   7836431
REFERENCE   8  (bases 1 to 3955)
  AUTHORS   Baude,E.J., Dignam,S.S., Olsen,S.R., Reimann,E.M. and Uhler,M.D.
  TITLE     Glutamic acid 203 of the cAMP-dependent protein kinase catalytic
            subunit participates in the inhibition by two isoforms of the
            protein kinase inhibitor
  JOURNAL   J Biol Chem 269 (3), 2316-2323 (1994)
   PUBMED   7905001
REFERENCE   9  (bases 1 to 3955)
  AUTHORS   Knighton,D.R., Zheng,J.H., Ten Eyck,L.F., Ashford,V.A., Xuong,N.H.,
            Taylor,S.S. and Sowadski,J.M.
  TITLE     Crystal structure of the catalytic subunit of cyclic adenosine
            monophosphate-dependent protein kinase
  JOURNAL   Science 253 (5018), 407-414 (1991)
   PUBMED   1862342
REFERENCE   10 (bases 1 to 3955)
  AUTHORS   Olsen,S.R. and Uhler,M.D.
  TITLE     Isolation and characterization of cDNA clones for an inhibitor
            protein of cAMP-dependent protein kinase
  JOURNAL   J Biol Chem 266 (17), 11158-11162 (1991)
   PUBMED   1710219
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132094.3 and AC123876.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR12282455.19244556.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-118               AC132094.3         122793-122910
            119-233             AC123876.5         118879-118993       c
            234-421             AC123876.5         94869-95056         c
            422-599             AC123876.5         88192-88369         c
            600-3955            AC123876.5         80341-83696         c
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 1.96 cM"
     gene            1..3955
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /note="protein kinase inhibitor, alpha"
                     /db_xref="GeneID:18767"
                     /db_xref="MGI:MGI:104747"
     exon            1..118
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            119..233
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            234..421
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            422..599
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            600..3955
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3929..3934
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /note="hexamer: ATTAAA"
     polyA_site      3955
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /note="major polyA site"
ORIGIN      
        1 agcggccgct gcagctgcag gtggggcggg ggccttgcga gcccagagcc cagcacacgc
       61 ggccaggcgg gcaggactgc agccaggggc agccgggacg cgtctctcca tcctgaagct
      121 tcctgcatat ccaggcctga tgtccccaag aggaaatttc tggtagaaat ctgaagaata
      181 tttctaagaa aaaaataact caatgaattg agaccaagac agtttacaat aagatcttct
      241 taaactcagc agcaagagtg ggttagacaa accccacatg ttatggcagt ctacagagaa
      301 aaggaataat aggaagaaga caaggaaatt cttttataaa ataagacctg gattttaagc
      361 cttggtctgc atatgattta agggtcattt gaagtatgag acatttttaa ctgaaaatga
      421 gtccctgcta tgtggatatt tgttagcaat gactgatgtg gaaactacgt atgcagattt
      481 cattgcttca ggaagaacag gtagaagaaa tgcaatacat gatatcctgg tttcctctgc
      541 aagtggcaac agcaatgaat tagccttaaa actagcaggc cttgatatca acaagacaga
      601 aggtgaagat gatggacaga gaagctccac cgaacaaagt ggagaagccc agggagaagc
      661 agccaagtct gaaagctaac actccattct gacggtcatc aacatctgac aatgtggcaa
      721 atatcctggc gatgctcgga tgtattcgca gccacgggtg aaacggaggg gaagaaaatg
      781 gctgcgctgc acagcaagac actgttggtt gccatgtcac aagtggggat ggagcctgtg
      841 tctgcacaca gccttttccc cacctctgtc attagcaatg gttgaaatca cctgggctgt
      901 gcttggatct ccttcgtgta tgggtccttc cacttgaccg tataatgaaa tgcttgtaga
      961 gagtagcact gcctagctga tgattcctgt ggcatctggc ccctcatgat gtcagaggat
     1021 ttaattgtgt ctaattgaaa agagttggtt gaaccccaga gtttcagtat ctctggctgg
     1081 agtattgact cacaaaaaga accatccaga gagcacagtt agcattatgt ccctgtgtgt
     1141 gagtgctgtt acttacgccg ttttgtatcg tacagtacac ctgctctgca ctgccctttg
     1201 attgcttaca taaaacaaaa tgatgtacat gactgtgaat ttttataccg ctcatttttt
     1261 aaagggctgc ctttttaatt tcccatagtg ttgtggcgtg aacagggcag aaccttcttt
     1321 tgtaaaacac aactttcctc ttgataactg ccgagggtta catccactag actcactgag
     1381 cccactttgc tttattatac aggcatgtgg aaatatccat gaatgtgaat attgcctgag
     1441 ttcagtctta gtatctacac agaccagcgt tctgtctttc aatattgatt ttatttccaa
     1501 ataagtactt tcaactgata ctttaatttt tcattataag aattttttta agaatcaaca
     1561 aaataaacaa agaatgccag ataatgcaaa gagctagaaa tgttgtgctg tgaggatcac
     1621 ttttctcaca ctgctgggtg ccactgttta acgcaggagg acaacttgtg aaggaagtgt
     1681 tttcctcaaa gcctgtgggc acccatctca aaagtaaagc ccttgtgctg tggcccctct
     1741 cagccctttc atacccaaat gttgttatgc actttttaat gtttgtaaaa ttttactaat
     1801 aatttgtgtg aattgcattc agatataaaa attatcaccc aactacactt ttactgatgc
     1861 tgttgatggt ctacgctgtg ttttgacatc acaattcttg tatggggagt ttctgtggcc
     1921 tgtataaccc tctctggtca gtattatgaa accaactatc tttggtaata aatgaggatc
     1981 cagttagatg cccaaggttc acctggtgca tcataggagg caaataacag atgaaagaag
     2041 actttctcaa cagaagagcc tctaagtgtc ctggagcact agcatagcta cagttccagg
     2101 ttaagtaggt agaggtgttt caacaggcag acctgggtac ggtaggaact cgacctgagt
     2161 ctgtagttac agacaaggta gacaaggaag tctgcctata tcttcccata gagtgctttc
     2221 attactgact gggaatatat gaatctcatt tagtccccaa ctggatgttt tccagaaaaa
     2281 caagcactgt taagtatgcc tttctggata tagaacagta aatacattca atatgagagg
     2341 ccttgctttg atctaagtca ttggaggcta gctaaaattg aaacaaatct acctattgat
     2401 agactcaaag ttgtgaacct atggcctatt tgagacaatc tccgataatc tatttacaac
     2461 atccaacagt ttcaatgtcc ttgtttaact atgctgatag ctttcttgtg aataggaaga
     2521 tcattcttaa agttgtgtaa ttccacactg gtttcacatg cattttgtaa agtgttgaac
     2581 cagtgctaag actgtgtgga agggttatac ccctgtgtca tatactatta ctaatgcatg
     2641 cggtgccatg cttgtcttta aatatataag tagtgctaaa tgcagaagtc atagggaggt
     2701 tctgatttca tttgatttct tactgctact tggtgtcctg tattcaatcc tagagggatt
     2761 ctgtggctgg taagggaaaa gagataaaca ttctgaattt gtcacatcac ataaatttta
     2821 attggcaaaa gtgcatagaa tctcctggtt ttcatttaat acttatcatt tgcaagtaaa
     2881 taatactaca tagggaatga atctgtaaat gcagatggaa gtgtgggcag cccaaggaaa
     2941 ggggtgttag ttaactaccc cagctaagtc acttcagaac caaatgtacc attcagtttc
     3001 atcttatgcc cctttcacaa attacaatgt gaacatgaat aaggactaat ttttgcaaaa
     3061 ctaaaaagct aatttgagcc catgtttatc aacagaattt tgaatccaca tttcataaaa
     3121 tttaagctaa ttacacagtg tgtttctctg tatggaaaac tatagataat acactcagtc
     3181 agcttgcatt ttacagagaa agaatttaag ttactaaata gactagcctt tcttgggttt
     3241 gtacacatgt ctttgggaca aagacacatg tggttgagcc tacctttttt tgttgttgtt
     3301 gttgttttaa tactcaatta tagcttattt tgaatttatt taagacatgt aaactgtaaa
     3361 tttacatgct atgttcatgc tatgtatgtg aacaaatatt cttgcttttt atttaataac
     3421 ctagaaacac aaaattttcc attttaacac attaacaatg aatgagaagc tgtagaattt
     3481 gttgagctta acttttttaa actaagaaca ttttatgtta gtacatattc atcctgaatt
     3541 cacagaaatg ctttctgctc tataacagaa aatcatgacc tttatttttc aggaaatact
     3601 gacatagtag cccactgttc acaccctacc actgagtctt ccttaaatgt gacatcaagg
     3661 atccaatgta gaataaaatg acatttttat ctcttctcag aggaatacga aaaaaatgta
     3721 actgttgggc agcaaacttc taaatgaatt ctgcagtagt aagggaaaaa aatgtatagt
     3781 cattaaatta aacgaagaaa ggatccataa ttgattgtgt cttagaagcc tggaaatgtt
     3841 ctttcactga cttgtggcag cctgggaagt catctgtgag cttctatgac ttttgtattt
     3901 agcaatgttg catgctcaca taactgatat taaaagcagc acttgttttc tgaaa
//
