LOCUS       NM_001410524            3730 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus protein kinase inhibitor, alpha (Pkia), transcript
            variant 5, mRNA.
ACCESSION   NM_001410524
VERSION     NM_001410524.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3730)
  AUTHORS   Hoy,J.J., Salinas Parra,N., Park,J., Kuhn,S. and
            Iglesias-Bartolome,R.
  TITLE     Protein kinase A inhibitor proteins (PKIs) divert GPCR-Galphas-cAMP
            signaling toward EPAC and ERK activation and are involved in tumor
            growth
  JOURNAL   FASEB J 34 (10), 13900-13917 (2020)
   PUBMED   32830375
  REMARK    GeneRIF: Protein kinase A inhibitor proteins (PKIs) divert
            GPCR-Galphas-cAMP signaling toward EPAC and ERK activation and are
            involved in tumor growth.
REFERENCE   4  (bases 1 to 3730)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 3730)
  AUTHORS   Kim,J., Li,G., Walters,M.A., Taylor,S.S. and Veglia,G.
  TITLE     Uncoupling Catalytic and Binding Functions in the Cyclic
            AMP-Dependent Protein Kinase A
  JOURNAL   Structure 24 (3), 353-363 (2016)
   PUBMED   26833386
REFERENCE   6  (bases 1 to 3730)
  AUTHORS   Seasholtz,A.F., Gamm,D.M., Ballestero,R.P., Scarpetta,M.A. and
            Uhler,M.D.
  TITLE     Differential expression of mRNAs for protein kinase inhibitor
            isoforms in mouse brain
  JOURNAL   Proc Natl Acad Sci U S A 92 (5), 1734-1738 (1995)
   PUBMED   7878050
REFERENCE   7  (bases 1 to 3730)
  AUTHORS   Wen,W., Taylor,S.S. and Meinkoth,J.L.
  TITLE     The expression and intracellular distribution of the heat-stable
            protein kinase inhibitor is cell cycle regulated
  JOURNAL   J Biol Chem 270 (5), 2041-2046 (1995)
   PUBMED   7836431
REFERENCE   8  (bases 1 to 3730)
  AUTHORS   Baude,E.J., Dignam,S.S., Olsen,S.R., Reimann,E.M. and Uhler,M.D.
  TITLE     Glutamic acid 203 of the cAMP-dependent protein kinase catalytic
            subunit participates in the inhibition by two isoforms of the
            protein kinase inhibitor
  JOURNAL   J Biol Chem 269 (3), 2316-2323 (1994)
   PUBMED   7905001
REFERENCE   9  (bases 1 to 3730)
  AUTHORS   Knighton,D.R., Zheng,J.H., Ten Eyck,L.F., Ashford,V.A., Xuong,N.H.,
            Taylor,S.S. and Sowadski,J.M.
  TITLE     Crystal structure of the catalytic subunit of cyclic adenosine
            monophosphate-dependent protein kinase
  JOURNAL   Science 253 (5018), 407-414 (1991)
   PUBMED   1862342
REFERENCE   10 (bases 1 to 3730)
  AUTHORS   Olsen,S.R. and Uhler,M.D.
  TITLE     Isolation and characterization of cDNA clones for an inhibitor
            protein of cAMP-dependent protein kinase
  JOURNAL   J Biol Chem 266 (17), 11158-11162 (1991)
   PUBMED   1710219
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132094.3 and AC123876.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR3363658.4052085.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-118               AC132094.3         122793-122910
            119-196             AC123876.5         139068-139145       c
            197-374             AC123876.5         88192-88369         c
            375-3730            AC123876.5         80341-83696         c
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 1.96 cM"
     gene            1..3730
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /note="protein kinase inhibitor, alpha"
                     /db_xref="GeneID:18767"
                     /db_xref="MGI:MGI:104747"
     exon            1..118
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            119..196
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            197..374
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             224..454
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /note="PKI-alpha"
                     /codon_start=1
                     /product="cAMP-dependent protein kinase inhibitor alpha"
                     /protein_id="NP_001397453.1"
                     /db_xref="GeneID:18767"
                     /db_xref="MGI:MGI:104747"
                     /translation="MTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAG
                     LDINKTEGEDDGQRSSTEQSGEAQGEAAKSES"
     exon            375..3730
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3704..3709
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /note="hexamer: ATTAAA"
     polyA_site      3730
                     /gene="Pkia"
                     /gene_synonym="PKIalpha"
                     /note="major polyA site"
ORIGIN      
        1 agcggccgct gcagctgcag gtggggcggg ggccttgcga gcccagagcc cagcacacgc
       61 ggccaggcgg gcaggactgc agccaggggc agccgggacg cgtctctcca tcctgaaggc
      121 cctggactct gccaggcatt tgatttgcca agtagaagac acaagaaacc cactttacta
      181 taatatgctt taacagtccc tgctatgtgg atatttgtta gcaatgactg atgtggaaac
      241 tacgtatgca gatttcattg cttcaggaag aacaggtaga agaaatgcaa tacatgatat
      301 cctggtttcc tctgcaagtg gcaacagcaa tgaattagcc ttaaaactag caggccttga
      361 tatcaacaag acagaaggtg aagatgatgg acagagaagc tccaccgaac aaagtggaga
      421 agcccaggga gaagcagcca agtctgaaag ctaacactcc attctgacgg tcatcaacat
      481 ctgacaatgt ggcaaatatc ctggcgatgc tcggatgtat tcgcagccac gggtgaaacg
      541 gaggggaaga aaatggctgc gctgcacagc aagacactgt tggttgccat gtcacaagtg
      601 gggatggagc ctgtgtctgc acacagcctt ttccccacct ctgtcattag caatggttga
      661 aatcacctgg gctgtgcttg gatctccttc gtgtatgggt ccttccactt gaccgtataa
      721 tgaaatgctt gtagagagta gcactgccta gctgatgatt cctgtggcat ctggcccctc
      781 atgatgtcag aggatttaat tgtgtctaat tgaaaagagt tggttgaacc ccagagtttc
      841 agtatctctg gctggagtat tgactcacaa aaagaaccat ccagagagca cagttagcat
      901 tatgtccctg tgtgtgagtg ctgttactta cgccgttttg tatcgtacag tacacctgct
      961 ctgcactgcc ctttgattgc ttacataaaa caaaatgatg tacatgactg tgaattttta
     1021 taccgctcat tttttaaagg gctgcctttt taatttccca tagtgttgtg gcgtgaacag
     1081 ggcagaacct tcttttgtaa aacacaactt tcctcttgat aactgccgag ggttacatcc
     1141 actagactca ctgagcccac tttgctttat tatacaggca tgtggaaata tccatgaatg
     1201 tgaatattgc ctgagttcag tcttagtatc tacacagacc agcgttctgt ctttcaatat
     1261 tgattttatt tccaaataag tactttcaac tgatacttta atttttcatt ataagaattt
     1321 ttttaagaat caacaaaata aacaaagaat gccagataat gcaaagagct agaaatgttg
     1381 tgctgtgagg atcacttttc tcacactgct gggtgccact gtttaacgca ggaggacaac
     1441 ttgtgaagga agtgttttcc tcaaagcctg tgggcaccca tctcaaaagt aaagcccttg
     1501 tgctgtggcc cctctcagcc ctttcatacc caaatgttgt tatgcacttt ttaatgtttg
     1561 taaaatttta ctaataattt gtgtgaattg cattcagata taaaaattat cacccaacta
     1621 cacttttact gatgctgttg atggtctacg ctgtgttttg acatcacaat tcttgtatgg
     1681 ggagtttctg tggcctgtat aaccctctct ggtcagtatt atgaaaccaa ctatctttgg
     1741 taataaatga ggatccagtt agatgcccaa ggttcacctg gtgcatcata ggaggcaaat
     1801 aacagatgaa agaagacttt ctcaacagaa gagcctctaa gtgtcctgga gcactagcat
     1861 agctacagtt ccaggttaag taggtagagg tgtttcaaca ggcagacctg ggtacggtag
     1921 gaactcgacc tgagtctgta gttacagaca aggtagacaa ggaagtctgc ctatatcttc
     1981 ccatagagtg ctttcattac tgactgggaa tatatgaatc tcatttagtc cccaactgga
     2041 tgttttccag aaaaacaagc actgttaagt atgcctttct ggatatagaa cagtaaatac
     2101 attcaatatg agaggccttg ctttgatcta agtcattgga ggctagctaa aattgaaaca
     2161 aatctaccta ttgatagact caaagttgtg aacctatggc ctatttgaga caatctccga
     2221 taatctattt acaacatcca acagtttcaa tgtccttgtt taactatgct gatagctttc
     2281 ttgtgaatag gaagatcatt cttaaagttg tgtaattcca cactggtttc acatgcattt
     2341 tgtaaagtgt tgaaccagtg ctaagactgt gtggaagggt tatacccctg tgtcatatac
     2401 tattactaat gcatgcggtg ccatgcttgt ctttaaatat ataagtagtg ctaaatgcag
     2461 aagtcatagg gaggttctga tttcatttga tttcttactg ctacttggtg tcctgtattc
     2521 aatcctagag ggattctgtg gctggtaagg gaaaagagat aaacattctg aatttgtcac
     2581 atcacataaa ttttaattgg caaaagtgca tagaatctcc tggttttcat ttaatactta
     2641 tcatttgcaa gtaaataata ctacataggg aatgaatctg taaatgcaga tggaagtgtg
     2701 ggcagcccaa ggaaaggggt gttagttaac taccccagct aagtcacttc agaaccaaat
     2761 gtaccattca gtttcatctt atgccccttt cacaaattac aatgtgaaca tgaataagga
     2821 ctaatttttg caaaactaaa aagctaattt gagcccatgt ttatcaacag aattttgaat
     2881 ccacatttca taaaatttaa gctaattaca cagtgtgttt ctctgtatgg aaaactatag
     2941 ataatacact cagtcagctt gcattttaca gagaaagaat ttaagttact aaatagacta
     3001 gcctttcttg ggtttgtaca catgtctttg ggacaaagac acatgtggtt gagcctacct
     3061 ttttttgttg ttgttgttgt tttaatactc aattatagct tattttgaat ttatttaaga
     3121 catgtaaact gtaaatttac atgctatgtt catgctatgt atgtgaacaa atattcttgc
     3181 tttttattta ataacctaga aacacaaaat tttccatttt aacacattaa caatgaatga
     3241 gaagctgtag aatttgttga gcttaacttt tttaaactaa gaacatttta tgttagtaca
     3301 tattcatcct gaattcacag aaatgctttc tgctctataa cagaaaatca tgacctttat
     3361 ttttcaggaa atactgacat agtagcccac tgttcacacc ctaccactga gtcttcctta
     3421 aatgtgacat caaggatcca atgtagaata aaatgacatt tttatctctt ctcagaggaa
     3481 tacgaaaaaa atgtaactgt tgggcagcaa acttctaaat gaattctgca gtagtaaggg
     3541 aaaaaaatgt atagtcatta aattaaacga agaaaggatc cataattgat tgtgtcttag
     3601 aagcctggaa atgttctttc actgacttgt ggcagcctgg gaagtcatct gtgagcttct
     3661 atgacttttg tatttagcaa tgttgcatgc tcacataact gatattaaaa gcagcacttg
     3721 ttttctgaaa
//
