LOCUS       NM_001025309            4706 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus praja ring finger ubiquitin ligase 2 (Pja2),
            transcript variant 1, mRNA.
ACCESSION   NM_001025309
VERSION     NM_001025309.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Rinaldi,L., Chiuso,F., Senatore,E., Borzacchiello,D., Lignitto,L.,
            Iannucci,R., Donne,R.D., Fuggi,M., Reale,C., Russo,F., Russo,N.A.,
            Giurato,G., Rizzo,F., Sellitto,A., Santangelo,M., De Biase,D.,
            Paciello,O., D'Ambrosio,C., Amente,S., Garbi,C., Dalla,E.,
            Scaloni,A., Weisz,A., Ambrosino,C., Insabato,L. and Feliciello,A.
  TITLE     Downregulation of praja2 restrains endocytosis and boosts tyrosine
            kinase receptors in kidney cancer
  JOURNAL   Commun Biol 7 (1), 208 (2024)
   PUBMED   38379085
  REMARK    GeneRIF: Downregulation of praja2 restrains endocytosis and boosts
            tyrosine kinase receptors in kidney cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4706)
  AUTHORS   Gong,M., Ye,S., Li,W.X., Zhang,J., Liu,Y., Zhu,J., Lv,W., Zhang,H.,
            Wang,J., Lu,A. and He,K.
  TITLE     Regulatory function of praja ring finger ubiquitin ligase 2
            mediated by the P2rx3/P2rx7 axis in mouse hippocampal neuronal
            cells
  JOURNAL   Am J Physiol Cell Physiol 318 (6), C1123-C1135 (2020)
   PUBMED   32267716
  REMARK    GeneRIF: Pja2 regulated hippocampal neuronal cell development and
            the expression of Alzheimer disease marker genes by inhibiting
            P2rx3/promoting P2rx7 expression.
REFERENCE   4  (bases 1 to 4706)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 4706)
  AUTHORS   Rinaldi,L., Delle Donne,R., Sepe,M., Porpora,M., Garbi,C.,
            Chiuso,F., Gallo,A., Parisi,S., Russo,L., Bachmann,V., Huber,R.G.,
            Stefan,E., Russo,T. and Feliciello,A.
  TITLE     praja2 regulates KSR1 stability and mitogenic signaling
  JOURNAL   Cell Death Dis 7 (5), e2230 (2016)
   PUBMED   27195677
  REMARK    GeneRIF: praja2 regulates KSR1 stability and mitogenic signaling.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4706)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 4706)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   8  (bases 1 to 4706)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 4706)
  AUTHORS   Sasaki,A., Masuda,Y., Iwai,K., Ikeda,K. and Watanabe,K.
  TITLE     A RING finger protein Praja1 regulates Dlx5-dependent transcription
            through its ubiquitin ligase activity for the Dlx/Msx-interacting
            MAGE/Necdin family protein, Dlxin-1
  JOURNAL   J Biol Chem 277 (25), 22541-22546 (2002)
   PUBMED   11959851
REFERENCE   10 (bases 1 to 4706)
  AUTHORS   Yu,P., Chen,Y., Tagle,D.A. and Cai,T.
  TITLE     PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human
            X chromosome gene abundantly expressed in brain
  JOURNAL   Genomics 79 (6), 869-874 (2002)
   PUBMED   12036302
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY065517.1 and AK122282.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK122282.1, SRR1660819.35498.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                BY065517.1         5-45
            42-4706             AK122282.1         1-4665
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 33.24 cM"
     gene            1..4706
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /note="praja ring finger ubiquitin ligase 2"
                     /db_xref="GeneID:224938"
                     /db_xref="MGI:MGI:2159342"
     exon            1..88
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /inference="alignment:Splign:2.1.0"
     exon            89..206
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /inference="alignment:Splign:2.1.0"
     CDS             176..2299
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /EC_number="2.3.2.27"
                     /note="isoform a is encoded by transcript variant 1;
                     protein carrying the RING-H2 sequence motif;
                     neurodegeneration associated protein 1; E3
                     ubiquitin-protein ligase Praja2; praja2; E3
                     ubiquitin-protein ligase Praja-2; praja-2; RING finger
                     protein 131; RING-type E3 ubiquitin transferase Praja-2;
                     praja 2, RING-H2 motif containing"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Praja-2 isoform a"
                     /protein_id="NP_001020480.1"
                     /db_xref="CCDS:CCDS37675.1"
                     /db_xref="GeneID:224938"
                     /db_xref="MGI:MGI:2159342"
                     /translation="MSQYTEKEPSVMDQESSKAAWPKPAGGYQTITGRRYGRRHAYVS
                     FKPCMTRHERSLGRAGDDYEVLELDDVPKENTSGSSSLDQVHPALPNEPTVEKSETEI
                     STCGPALNQSTESSPSIATVCHSEEVRETLESNTNLHNRTETEHTPAVCNVSSVQNGI
                     MLVHTDSYDPDSKHDENGSLQLGAEAVEGGRHQKGLGRAVFELENGEAEIYADLSPSV
                     PSLNGEISEAFEELDSAPLEKSSTADAELVHQNGQEFQRSSEDGVVRKRRQDDTDQGR
                     QTENSTEDADCAPGHVEQNTSDRANHHGSSPEQVVRPKVRKVISSSQVDQEIGFNRHE
                     AKQRSVQRWREALEVEECSSDDPIIKCDDYDGDHDCMFLTPTYSRVTQRETERNRVTS
                     ENGATASGRQESRDNAFWNACGEYYQLFDKDEDSSECSDGEWSASLPHRFSGTEKDQS
                     SSDDSWETLPGKDENDPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNE
                     SSSDEGNEPANEFAQPEAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAIS
                     YVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDH
                     TAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVI
                     DASAAASSDPDPDASPANDNAEEAP"
     exon            207..407
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /inference="alignment:Splign:2.1.0"
     exon            408..1452
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1638
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1824
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1936
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1937..2051
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2052..2173
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2174..4706
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2683..2688
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
                     /note="hexamer: AATAAA"
     polyA_site      2703
                     /gene="Pja2"
                     /gene_synonym="mKIAA0438; Neurodap1"
ORIGIN      
        1 gggggaggag tcggaggcgg agaagaaggc ggtggtggcg ggtggaggat cgagcgctgt
       61 tctcgctctg gagccgctgc acatctcgga attattctca gcttattcac tgtgcaagaa
      121 aaaaacaaat tacatccata attctactga actggaatca tagacgctgc catatatgtc
      181 acagtacact gaaaaagagc catcagtaat ggatcaagaa tccagcaagg ctgcttggcc
      241 taaaccagca ggaggatatc agactatcac aggcaggaga tatggaagaa gacatgcgta
      301 tgtcagtttt aagccatgta tgaccaggca tgaaagaagc ttgggtcggg ctggtgatga
      361 ctatgaagtg ttggaactag acgatgttcc aaaggagaac acctcaggtt ccagttcatt
      421 ggatcaagtc catcctgctt taccaaatga acctacagtt gaaaaaagtg aaacagaaat
      481 ttctacttgt ggtccagcac tgaatcaaag cactgagagc agtccatcca ttgccacagt
      541 atgtcatagt gaggaagtca gggagacctt agaaagcaat acaaaccttc ataatcgcac
      601 tgagacagag catactccag cagtttgtaa tgtctcaagt gtccagaatg gaattatgtt
      661 ggttcatact gactcttatg atccagacag caagcatgat gagaatggct ctcttcaact
      721 tggcgctgaa gctgtggaag gtggtagaca tcagaagggg ttaggccgtg cagtctttga
      781 gctggaaaat ggagaggcag agatctacgc tgacctgtca ccatcagtcc cctctctcaa
      841 cggtgaaata agtgaggcgt ttgaagagct agattcagca cctttagaga aaagttctac
      901 tgccgatgct gagcttgtcc atcagaatgg gcaggaattt cagaggtctt ctgaagatgg
      961 cgttgttaga aagaggcgac aagacgatac tgatcaggga agacagacag aaaattcaac
     1021 tgaagatgca gactgtgctc caggccatgt tgagcaaaat actagtgata gagccaacca
     1081 ccatggaagt tctcctgaac aggtagtaag gcccaaagtt agaaaagtga taagttcaag
     1141 ccaggtggac caagaaattg gttttaatag gcacgaggct aagcagagaa gcgttcagag
     1201 atggagagag gctttggaag tggaggagtg cagttcagat gaccctataa taaagtgtga
     1261 cgattatgat ggagaccacg actgcatgtt cctaactcca acttactcaa gagttacaca
     1321 aagggagaca gaacggaatc gtgtaacatc agaaaatgga gctacagctt caggacggca
     1381 agaatctcgg gacaatgcct tttggaatgc ctgcggagag tattaccaac tctttgacaa
     1441 agatgaagac agttcagagt gcagtgatgg ggaatggtct gcttctctgc ctcaccgatt
     1501 ttctggcaca gaaaaagacc agtcctcaag cgatgacagc tgggaaactc tgccaggaaa
     1561 ggatgagaat gaccctgagc tgcagagtga tagtagtggc cccgaggaag aaaaccaaga
     1621 gttgtctctt caggaggggg aacagacatc cttggaagag ggagagatcc cttggttaca
     1681 gtacaatgag gtcaatgaga gcagcagcga tgaagggaat gagcctgcca atgagtttgc
     1741 acagccggaa gctttcatgt tggatgggaa caacaacctt gaggacgact cgagcgtgag
     1801 tgaagacttg gatgtggact ggagcctgtt tgatggtttt gcagacggac ttggagttgc
     1861 tgaagctatt tcttatgtgg atcctcagtt ccttacctac atggcactag aagagcgctt
     1921 agcccaggct atggagactg cgctggcaca tttagagtcc cttgcagtag acgttgaggt
     1981 ggccaatcca cctgccagta aggaaagcat tgatgggctt ccagagactc tggttctaga
     2041 ggaccacacc gccatcggcc aggagcagtg ctgtcccatc tgctgcagcg agtacatcaa
     2101 ggatgacatt gccacagagc tgccttgcca tcacttcttt cataagccct gtgtctccat
     2161 ctggctacag aagtccggaa catgccctgt gtgccgccgt cacttcccac ctgcagttat
     2221 tgacgcatct gcagctgctt cctctgaccc tgaccctgat gcctcgcctg caaatgacaa
     2281 tgctgaagaa gccccataaa ccatcacgtg gaaatgagat caaaatccta cacagtaaat
     2341 ctgcaaattc cttctaaatc tgatgtgcaa ataattatat ataaatatat ttaaaatgct
     2401 ctatatagta tatgccatag tttaggaaga gaatattacc ctttctaaac tgaacttagg
     2461 tttgcagaac acactaaaca tttccagctg agtgttgaag ccgtgcatcc cttttcttcg
     2521 gttgaccatg gtgttgattg tgacgtcagc agtcatctct gtggcacacc tttccctttt
     2581 gtctaaagtg ctgccactaa gagatcatcc ttacgctccc ttcccacatg agatgtgaag
     2641 actgtggtca caacagtagt agtacaactt ggtttcattg aaaataaact gaattctaaa
     2701 gcatgctttc tcactggtcc ctttgctttt gctacatcga aacctcttgg tttataacaa
     2761 tactgagaag gttaagattg gagcttttca gaatgccaaa caaagactaa agttttgacc
     2821 agaacaccaa aaaataaaaa aataataata aaaatacatc ttggatgtaa acctcctgaa
     2881 atggtcacgt aataaactct tcagtgagca ctggatgtgt ttaacggcga gtaaaacatg
     2941 tgaataatcg gatgtgctcc atgcatggca cagttcatgg tttggcctta tgaagcactg
     3001 tctaagcccc gatccagtct gcttctactt gttaaatact tgaaggtcca ggacactctt
     3061 gacatgtttg tttaagaagt tggatatagt tagcttaaag tagtttgggt tctactttag
     3121 aaggtctgaa ggtttgcaca tggtcatgtt cataactata tcacccccat aagaggtgca
     3181 cagcttttag gtgatcacag gagtgggcaa gtgtgtatag acatcggtgt aacgggtcgc
     3241 tcacgagtga aaccagacat cagcactggg gaacaatggc ttagacggca tagagcgagt
     3301 gtgcacccac ctgctcctct gctcatagtg tcgggaatgt ttatgtagct tccgaaatgc
     3361 agtttcattt aatagctctg tgtaatatct ctgagcattt tatattcttt ctttttttcc
     3421 agtcttaaaa ataaatgaat tttcactttt ggcacatttg aggcttaaat ataagaaaca
     3481 taacacttgc tttctgattt ttgcatatat tgtaaatgtg gctggtattt acagcaaata
     3541 ctgtgtatcc ttttatgggt aaaacacaag tgaacattgc atgcaagtaa tgtggtgaat
     3601 ttgtaattta gaggttttct ggggcttctg tgactttgga tgcttaaatt gaggccttaa
     3661 tgttgctcta gctagcatgt ttcctcctga tgtatatagt cagcgttgta taaactaatc
     3721 tctgcttgtt ttctactctg tgatctttcc ataccgtatt tcattgatca gttagtgtca
     3781 gaagtcatac cagagtaaca ttagttccta tgtgtatact gtggatattt gtgaattgtg
     3841 tggtttgttt gtttgttttc ttttatgtac tgttgactag ttatggcact tactgattaa
     3901 acttacactg ctgcagattg ctgtgtaaca agccacatgt tatgtttagt cactattatg
     3961 ggaccttggt ttttcttcta gatagcagct gtcccaaaga aaatatttct tctttgccta
     4021 ttaagatgta gctattatct gccagttgtt aaaggggttt ggttccaaac tcaaccagag
     4081 agttgaacgt aagaaagctg ttgtactttt tgcttcccat ctgttaccgt ccctcactgt
     4141 tggctcctta ctacgcatac acagctgctg tggggagaaa gctgaatgag acttgtttca
     4201 atttttttag aaaagaaaat gaagttttag gactttcatg gtaacaaacc tgacaagttc
     4261 agaccccagt atattatttg atcttatctt ggatcctttt gaaaagttaa gaatatatga
     4321 aggtaaatta gaaatagtga aaatattgat aaaatgtcat ttatcttatt tctctatttt
     4381 atgttgttac ttggcctatt aattttaatt ttttgaacat tttcttattt ctcgtatgtg
     4441 aatgctgata tttaaagttc aactcatgtg gatttctttt gtgtttctta gttgttatct
     4501 ctaagctaac taacttttaa gattatttgt gatatggatt tatatataag ctgttaaata
     4561 tatatgaact gttaaaacgg aatgcaaggt tttcaaaagc ccaggtctaa ctgtgatgat
     4621 tggtttattg gtctacaatc ccagcctggc attttccgtg taaatcataa acaataaaca
     4681 ggatatactc ggtgttcatt tctaat
//
