LOCUS       NM_001368831            3257 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus piwi-like RNA-mediated gene silencing 4 (Piwil4),
            transcript variant 1, mRNA.
ACCESSION   NM_001368831 XM_017313437
VERSION     NM_001368831.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Dias Mirandela,M., Zoch,A., Leismann,J., Webb,S., Berrens,R.V.,
            Valsakumar,D., Kabayama,Y., Auchynnikava,T., Schito,M.,
            Chowdhury,T., MacLeod,D., Xiang,X., Zou,J., Rappsilber,J.,
            Allshire,R.C., Voigt,P., Cook,A.G., Barau,J. and O'Carroll,D.
  TITLE     Two-factor authentication underpins the precision of the piRNA
            pathway
  JOURNAL   Nature 634 (8035), 979-985 (2024)
   PUBMED   39294378
  REMARK    GeneRIF: Two-factor authentication underpins the precision of the
            piRNA pathway.
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Wen,Y., Zhou,S., Gui,Y., Li,Z., Yin,L., Xu,W., Feng,S., Ma,X.,
            Gan,S., Xiong,M., Dong,J., Cheng,K., Wang,X. and Yuan,S.
  TITLE     hnRNPU is required for spermatogonial stem cell pool establishment
            in mice
  JOURNAL   Cell Rep 43 (4), 114113 (2024)
   PUBMED   38625792
REFERENCE   3  (bases 1 to 3257)
  AUTHORS   Zoch,A., Konieczny,G., Auchynnikava,T., Stallmeyer,B., Rotte,N.,
            Heep,M., Berrens,R.V., Schito,M., Kabayama,Y., Schopp,T.,
            Kliesch,S., Houston,B., Nagirnaja,L., O'Bryan,M.K., Aston,K.I.,
            Conrad,D.F., Rappsilber,J., Allshire,R.C., Cook,A.G., Tuttelmann,F.
            and O'Carroll,D.
  TITLE     C19ORF84 connects piRNA and DNA methylation machineries to defend
            the mammalian germ line
  JOURNAL   Mol Cell 84 (6), 1021-1035 (2024)
   PUBMED   38359823
REFERENCE   4  (bases 1 to 3257)
  AUTHORS   Ren,J.S., Bai,W., Ding,J.J., Zhao,Y., Wang,S.Y., Chen,X. and
            Jiang,Q.
  TITLE     The role of PIWIL4 and piRNAs in the development of choroidal
            neovascularization
  JOURNAL   Genomics 115 (3), 110615 (2023)
   PUBMED   36934857
  REMARK    GeneRIF: The role of PIWIL4 and piRNAs in the development of
            choroidal neovascularization.
REFERENCE   5  (bases 1 to 3257)
  AUTHORS   Ramakrishna,N.B., Battistoni,G., Surani,M.A., Hannon,G.J. and
            Miska,E.A.
  TITLE     Mouse primordial germ-cell-like cells lack piRNAs
  JOURNAL   Dev Cell 57 (23), 2661-2668 (2022)
   PUBMED   36473462
REFERENCE   6  (bases 1 to 3257)
  AUTHORS   Aravin,A.A., Sachidanandam,R., Bourc'his,D., Schaefer,C., Pezic,D.,
            Toth,K.F., Bestor,T. and Hannon,G.J.
  TITLE     A piRNA pathway primed by individual transposons is linked to de
            novo DNA methylation in mice
  JOURNAL   Mol Cell 31 (6), 785-799 (2008)
   PUBMED   18922463
REFERENCE   7  (bases 1 to 3257)
  AUTHORS   Kuramochi-Miyagawa,S., Watanabe,T., Gotoh,K., Totoki,Y., Toyoda,A.,
            Ikawa,M., Asada,N., Kojima,K., Yamaguchi,Y., Ijiri,T.W., Hata,K.,
            Li,E., Matsuda,Y., Kimura,T., Okabe,M., Sakaki,Y., Sasaki,H. and
            Nakano,T.
  TITLE     DNA methylation of retrotransposon genes is regulated by Piwi
            family members MILI and MIWI2 in murine fetal testes
  JOURNAL   Genes Dev 22 (7), 908-917 (2008)
   PUBMED   18381894
  REMARK    GeneRIF: Data strongly suggest that MILI and MIWI2 play essential
            roles in establishing de novo DNA methylation of retrotransposons
            in fetal male germ cells.
REFERENCE   8  (bases 1 to 3257)
  AUTHORS   Carmell,M.A., Girard,A., van de Kant,H.J., Bourc'his,D.,
            Bestor,T.H., de Rooij,D.G. and Hannon,G.J.
  TITLE     MIWI2 is essential for spermatogenesis and repression of
            transposons in the mouse male germline
  JOURNAL   Dev Cell 12 (4), 503-514 (2007)
   PUBMED   17395546
REFERENCE   9  (bases 1 to 3257)
  AUTHORS   Costa,Y., Speed,R.M., Gautier,P., Semple,C.A., Maratou,K.,
            Turner,J.M. and Cooke,H.J.
  TITLE     Mouse MAELSTROM: the link between meiotic silencing of unsynapsed
            chromatin and microRNA pathway?
  JOURNAL   Hum Mol Genet 15 (15), 2324-2334 (2006)
   PUBMED   16787967
REFERENCE   10 (bases 1 to 3257)
  AUTHORS   Carmell,M.A., Xuan,Z., Zhang,M.Q. and Hannon,G.J.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT030247.6.
            
            On Mar 1, 2019 this sequence version replaced XM_017313437.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMN00849380 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-215               CT030247.6         77836-78050
            216-279             CT030247.6         79054-79117
            280-411             CT030247.6         81567-81698
            412-626             CT030247.6         83851-84065
            627-748             CT030247.6         88512-88633
            749-829             CT030247.6         89784-89864
            830-1027            CT030247.6         90982-91179
            1028-1139           CT030247.6         92532-92643
            1140-1263           CT030247.6         98348-98471
            1264-1381           CT030247.6         100155-100272
            1382-1493           CT030247.6         102669-102780
            1494-1678           CT030247.6         103481-103665
            1679-1751           CT030247.6         106166-106238
            1752-1952           CT030247.6         108396-108596
            1953-2056           CT030247.6         109543-109646
            2057-2127           CT030247.6         112229-112299
            2128-2281           CT030247.6         113449-113602
            2282-2410           CT030247.6         114790-114918
            2411-2558           CT030247.6         115336-115483
            2559-3257           CT030247.6         116034-116732
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 4.25 cM"
     gene            1..3257
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /note="piwi-like RNA-mediated gene silencing 4"
                     /db_xref="GeneID:330890"
                     /db_xref="MGI:MGI:3041167"
     exon            1..215
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     CDS             129..2675
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     piwi-like protein 4; piwi-like 4; piwi-like homolog 4"
                     /codon_start=1
                     /product="piwi-like protein 4 isoform 1"
                     /protein_id="NP_001355760.1"
                     /db_xref="CCDS:CCDS22824.2"
                     /db_xref="GeneID:330890"
                     /db_xref="MGI:MGI:3041167"
                     /translation="MSGRARVRARGITTGHSAREVGRSSRDLMVTSASPGDSEAGGGT
                     SVISQPYELGVSSGDGGRTFMERRGKGRQDFEELGVCTREKLTHVKDCKTGSSGIPVR
                     LVTNLFNLDLPQDWQLYQYHVTYSPDLASRRLRIALLYNHSILSDKAKAFDGASLFLS
                     EKLDQKVTELTSETQRGETIKITLTLTSKLFPNSPVCIQFFNVIFRKILKNLSMYQIG
                     RNFYKPSEPVEIPQYKLSLWPGFAISVSHFESKLLFNADVNYKVLRNETVLDFMTDLC
                     LRTGMSCFTEMCHKQLVGLVVLTRYNNKTYRIDDIDWSVKPTQAFQKRDGSEVTYVDY
                     YKQQYDITLSDLNQPVLVSLLKRKRNDNSEPQMVHLMPELCFLTGLSSQATSDFRLMK
                     AVAEETRLSPVGRQQQLARLVDDIQRNPVARFELETWGLHFGSQLSLTGRVVPSEKIL
                     LQDHTCQPAFAADWSKDMRSCKVLSSQPLNRWLIVCCNRAEHLIEAFLSCLRRVGGSM
                     GFNVGYPKIIKVDETPAAFLRAIQVHGDPDVQLVMCILPSNQKNYYDSIKKYLSSDCP
                     VPSQCVLTRTLNKQGTMLSVATKIAMQMTCKLGGELWSVEIPLKSLMVVGIDICRDAL
                     NKNVVVVGFVASINSRITRWFSRCVLQRTAADIADCLKVCMTGALNRWYRHNHDLPAR
                     IVVYRDGVGNGQLKAVLEYEVPQLLKSVTECGSDARSCRLSVVVVRKRCLLRLFASTD
                     HTVQNPPLGTVVDSEATRPEWYDFYLISQTANRGTVSPTHYNVIYDDNALKPDHMQRL
                     TFKLCHLYYNWQGLISVPAPCQYAHKLTFLVAQSVHKEPSLELANNLFYL"
     exon            216..279
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            280..411
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            412..626
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            627..748
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            749..829
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            830..1027
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1139
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1263
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1381
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1493
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1678
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            1679..1751
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1952
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2056
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..2127
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2281
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2410
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            2411..2558
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     exon            2559..3257
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3234..3239
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /note="hexamer: AATAAA"
     polyA_site      3255
                     /gene="Piwil4"
                     /gene_synonym="9230101H05Rik; mAgo5; Miwi2"
                     /note="major polyA site"
ORIGIN      
        1 gtagtaacta tcttgggaag cttgaatact cccaaactcc gagtcacagc cacaccgtct
       61 cttttgccaa agagaaaaca ctcctgttga ggctcaccag ccctggaaag ccgctggcac
      121 ccaggaacat gagtggacgg gcccgtgtga gagcgcgagg catcaccact ggccacagtg
      181 ctagggaagt ggggcggtcc tcaagagacc tcatggttac atctgccagt cctggggaca
      241 gcgaggccgg aggtgggaca agtgtcattt cacagccata tgaacttggt gtctcttctg
      301 gtgatggtgg acgtaccttc atggaaagaa gaggcaaagg cagacaggac ttcgaagagt
      361 tgggtgtctg taccagggaa aagttgaccc acgtaaaaga ttgtaaaaca ggttctagcg
      421 gaatacctgt gcggctggtt acaaatctct ttaatttaga tttgccccag gattggcagc
      481 tgtaccagta ccatgtcacg tacagcccag atttagcctc taggaggctg aggattgctt
      541 tgctttataa tcacagcata ctctcagaca aggcaaaagc atttgacggt gccagccttt
      601 tcctctcaga aaagctggac cagaaggtca cagaactgac aagtgaaaca caaagaggtg
      661 agactataaa gataacgctc accctaacga gcaagctctt cccaaactcc cctgtgtgca
      721 ttcagttctt caatgtcatc ttcagaaaga tcttgaaaaa cttgagtatg taccaaattg
      781 gacggaactt ctataagcct tcagaaccag tggagatccc tcagtacaaa ttgtccctgt
      841 ggcctgggtt tgccatttct gtgtctcact ttgaaagcaa gctcctattc aatgctgacg
      901 tgaactacaa agtcctccgc aatgagaccg ttctggactt catgactgat ctctgcctca
      961 gaactggcat gtcttgcttc accgagatgt gccacaaaca actagtgggg ctcgttgtcc
     1021 ttaccagata caacaacaaa acatatcgta tcgatgacat tgactggtct gtgaagccca
     1081 cacaggcctt tcagaagcgg gacggctcag aggtcacata tgtggattac tacaagcagc
     1141 aatatgatat caccttatct gacctaaatc agccagtgct tgttagtctg ttgaaaagaa
     1201 agagaaatga taactctgag cctcagatgg tccacctgat gccagagctc tgctttctca
     1261 caggcctgag cagccaagca acctcagatt tccgcctgat gaaggcagta gctgaagaga
     1321 ctcggctcag tcctgtggga aggcagcagc agctggcccg actcgtggat gacatccaga
     1381 ggaacccagt ggctcggttt gagcttgaga cctggggatt gcattttgga agccagctat
     1441 ccctgaccgg ccgggttgtt ccctctgaaa aaatcctgct gcaggaccac acatgtcaac
     1501 ctgcatttgc tgctgactgg tccaaagata tgcgatcttg caaagttttg agttctcagc
     1561 ctttgaatag atggttgatc gtgtgctgta acagggctga gcacttgatt gaagcctttc
     1621 tgagctgtct gaggagagtt ggaggttcca tgggatttaa cgtgggctac cccaaaatca
     1681 taaaagtgga cgagacccca gcagcgttcc ttcgagccat ccaggtgcac ggcgaccccg
     1741 atgttcagtt ggtgatgtgc attctgcctt ctaatcagaa gaactattac gactccatta
     1801 aaaagtattt gagctctgac tgcccagtgc caagccagtg tgtgctgacc cggaccttga
     1861 ataagcaggg aacgatgctg agtgtggcca ccaagatcgc catgcagatg acctgcaaac
     1921 ttggcggaga gctgtggtct gtggagatcc cattgaagtc cctgatggtc gtgggtattg
     1981 atatctgcag agatgccctc aacaagaatg tggtggtcgt cgggtttgta gccagcatta
     2041 attccaggat caccaggtgg ttttcccgct gtgtccttca gagaacagcg gctgatattg
     2101 cagattgcct caaagtctgc atgactggtg ctctcaaccg gtggtacaga cacaaccatg
     2161 acttgccggc acggatagtc gtgtaccggg acggtgtagg caatggccag ctaaaggcag
     2221 ttttggaata tgaagtccca cagctactga aaagtgtaac agagtgcggc tcggatgcca
     2281 ggagctgcag actgtccgtg gttgtggtca ggaagagatg tctactgcgc ctctttgctt
     2341 cgactgacca cactgtgcag aaccccccac tcggcactgt tgtggactca gaagcaacac
     2401 gtccggagtg gtatgacttc tacctgatca gccagactgc taaccggggg actgttagtc
     2461 ccacccacta caacgttatc tatgatgaca atgccttgaa gcctgaccac atgcagcgac
     2521 tgaccttcaa actgtgccat ctctactaca actggcaggg cttaatcagt gtccccgcac
     2581 catgccaata tgcacacaag ctgaccttcc tggtggcaca aagtgtccac aaggaaccaa
     2641 gtctggaatt agccaataat cttttctacc tctgacaagt gagccagagg acggcttgag
     2701 actccgaaag cagtggctct gcgttgttca gatctgccta ccgctcaggc tcctgcggct
     2761 tacggggctt gggcctaact ttaagtattg ggaagggggg gttgttttgt tttgttttgt
     2821 tttgtttttt aaaaaaaaaa ctcatttgaa aaagttcaga acaaacctat ggggattttt
     2881 gtttcacttg tgtctaaaac caacaaagac aaacaaacag aacccacaga gtgatgggtt
     2941 ttcttgtggc tttgtcatgt gtcattttac tttggcctgg ttcagccttg tatttctctt
     3001 ctcttctctt ctcttctctt ctcttctctt cttttctttc tttctttttc ttttttttaa
     3061 gtcagtgctt gaggtaaatt ctcatgtggt acttgggata tatcatcaca gcttctaaaa
     3121 caatatttgt gtggtatttt gatggtacat agtggggttt aacttcagga aattatcaga
     3181 gtttttctaa acattgtaga gcattttgta gagtgggtta agtaaatatt gaaaataaag
     3241 aaaatctaag catcaca
//
