LOCUS       NM_021308               4930 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus piwi-like RNA-mediated gene silencing 2 (Piwil2),
            transcript variant 1, mRNA.
ACCESSION   NM_021308
VERSION     NM_021308.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Gao,J., Jing,J., Shang,G., Chen,C., Duan,M., Yu,W., Wang,K.,
            Luo,J., Song,M., Chen,K., Chen,C., Zhang,T. and Ding,D.
  TITLE     TDRD1 phase separation drives intermitochondrial cement assembly to
            promote piRNA biogenesis and fertility
  JOURNAL   Dev Cell 59 (20), 2704-2718 (2024)
   PUBMED   39029469
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Lu,X., Zhu,Q., Du,H., Gu,M. and Li,X.
  TITLE     PIWIL2 restrains the progression of thyroid cancer via interaction
            with miR-146a-3p
  JOURNAL   BMC Endocr Disord 23 (1), 184 (2023)
   PUBMED   37641092
  REMARK    GeneRIF: PIWIL2 restrains the progression of thyroid cancer via
            interaction with miR-146a-3p.
            Erratum:[BMC Endocr Disord. 2024 Nov 26;24(1):255. doi:
            10.1186/s12902-024-01786-z.. PMID: 39587564]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4930)
  AUTHORS   Gasperini,C., Tuntevski,K., Beatini,S., Pelizzoli,R., Lo Van,A.,
            Mangoni,D., Cossu,R.M., Pascarella,G., Bianchini,P., Bielefeld,P.,
            Scarpato,M., Pons-Espinal,M., Sanges,R., Diaspro,A.,
            Fitzsimons,C.P., Carninci,P., Gustincich,S. and De Pietri
            Tonelli,D.
  TITLE     Piwil2 (Mili) sustains neurogenesis and prevents cellular
            senescence in the postnatal hippocampus
  JOURNAL   EMBO Rep 24 (2), e53801 (2023)
   PUBMED   36472244
  REMARK    GeneRIF: Piwil2 (Mili) sustains neurogenesis and prevents cellular
            senescence in the postnatal hippocampus.
REFERENCE   4  (bases 1 to 4930)
  AUTHORS   Ramakrishna,N.B., Battistoni,G., Surani,M.A., Hannon,G.J. and
            Miska,E.A.
  TITLE     Mouse primordial germ-cell-like cells lack piRNAs
  JOURNAL   Dev Cell 57 (23), 2661-2668 (2022)
   PUBMED   36473462
REFERENCE   5  (bases 1 to 4930)
  AUTHORS   Lowe,M.G., Yen,M.R., Hsu,F.M., Hosohama,L., Hu,Z., Chitiashvili,T.,
            Hunt,T.J., Gorgy,I., Bernard,M., Wamaitha,S.E., Chen,P.Y. and
            Clark,A.T.
  TITLE     EED is required for mouse primordial germ cell differentiation in
            the embryonic gonad
  JOURNAL   Dev Cell 57 (12), 1482-1495 (2022)
   PUBMED   35679863
REFERENCE   6  (bases 1 to 4930)
  AUTHORS   Kuramochi-Miyagawa,S., Kimura,T., Ijiri,T.W., Isobe,T., Asada,N.,
            Fujita,Y., Ikawa,M., Iwai,N., Okabe,M., Deng,W., Lin,H., Matsuda,Y.
            and Nakano,T.
  TITLE     Mili, a mammalian member of piwi family gene, is essential for
            spermatogenesis
  JOURNAL   Development 131 (4), 839-849 (2004)
   PUBMED   14736746
REFERENCE   7  (bases 1 to 4930)
  AUTHORS   Sasaki,T., Shiohama,A., Minoshima,S. and Shimizu,N.
  TITLE     Identification of eight members of the Argonaute family in the
            human genome
  JOURNAL   Genomics 82 (3), 323-330 (2003)
   PUBMED   12906857
REFERENCE   8  (bases 1 to 4930)
  AUTHORS   Tan,C.H., Lee,T.C., Weeraratne,S.D., Korzh,V., Lim,T.M. and Gong,Z.
  TITLE     Ziwi, the zebrafish homologue of the Drosophila piwi:
            co-localization with vasa at the embryonic genital ridge and
            gonad-specific expression in the adults
  JOURNAL   Mech Dev 119 Suppl 1, S221-S224 (2002)
   PUBMED   14516689
REFERENCE   9  (bases 1 to 4930)
  AUTHORS   Kuramochi-Miyagawa,S., Kimura,T., Yomogida,K., Kuroiwa,A.,
            Tadokoro,Y., Fujita,Y., Sato,M., Matsuda,Y. and Nakano,T.
  TITLE     Two mouse piwi-related genes: miwi and mili
  JOURNAL   Mech Dev 108 (1-2), 121-133 (2001)
   PUBMED   11578866
REFERENCE   10 (bases 1 to 4930)
  AUTHORS   Wang,P.J., McCarrey,J.R., Yang,F. and Page,D.C.
  TITLE     An abundance of X-linked genes expressed in spermatogonia
  JOURNAL   Nat Genet 27 (4), 422-426 (2001)
   PUBMED   11279525
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122268.5, AB032605.1 and AC025669.19.
            
            On Jun 14, 2018 this sequence version replaced NM_021308.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB032605.1, BC138444.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AC122268.5         53427-53440         c
            15-4927             AB032605.1         1-4913
            4928-4930           AC025669.19        125171-125173       c
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 36.32 cM"
     gene            1..4930
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="piwi-like RNA-mediated gene silencing 2"
                     /db_xref="GeneID:57746"
                     /db_xref="MGI:MGI:1930036"
     exon            1..166
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            167..403
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..3127
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="isoform 1 is encoded by transcript variant 1; Miwi
                     like; piwi-like protein 2; Miwi likw; piwi-like homolog 2"
                     /codon_start=1
                     /product="piwi-like protein 2 isoform 1"
                     /protein_id="NP_067283.1"
                     /db_xref="CCDS:CCDS27252.1"
                     /db_xref="GeneID:57746"
                     /db_xref="MGI:MGI:1930036"
                     /translation="MDPVRPLFRGPTPVHPSQCVRMPGCWPQAPRPLEPAWGRAGPAG
                     RGLVFRKPEDSSPPLQPVQKDSVGLVSMFRGMGLDTAFRPPSKREVPPLGRGVLGRGL
                     SANMVRKDREEPRSSLPDPSVLAAGDSKLAEASVGWSRMLGRGSSEVSLLPLGRAASS
                     IGRGMDKPPSAFGLTARDPPRLPQPPALSPTSLHSADPPPVLTMERKEKELLVKQGSK
                     GTPQSLGLNLIKIQCHNEAVYQYHVTFSPSVECKSMRFGMLKDHQSVTGNVTAFDGSI
                     LYLPVKLQQVVELKSQRKTDDAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDM
                     KLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDSVLDVM
                     HAIYQQNKEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITF
                     LEYYSKNYGITVKEDDQPLLIHRPSERQNNHGMLLKGEILLLPELSFMTGIPEKMKKD
                     FRAMKDLTQQINLSPKQHHGALECLLQRISQNETASNELTRWGLSLHKDVHKIEGRLL
                     PMERINLRNTSFVTSEDLNWVKEVTRDASILTIPMHFWALFYPKRAMDQARELVNMLE
                     KIAGPIGMRISPPAWVELKDDRIETYIRTIQSLLGVEGKIQMVVCIIMGTRDDLYGAI
                     KKLCCVQSPVPSQVINVRTIGQPTRLRSVAQKILLQMNCKLGGELWGVDIPLKQLMVI
                     GMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKYY
                     EVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFDNYHPKMVVFVVQKKIST
                     NLYLAAPDHFVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANLS
                     PDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQILHHEPAIQLCGNLFFL"
     exon            404..491
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            492..630
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            631..837
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            838..948
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1066
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1191
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1272
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1386
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1575
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1660
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1750
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1891
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1892..2005
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2006..2194
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2296
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2297..2413
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2502
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2503..2608
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2609..2862
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2863..2970
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     exon            2971..4930
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4908..4913
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="hexamer: AATAAA"
     polyA_site      4928
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="major polyA site"
ORIGIN      
        1 gttattccag gccgagtgtg tgggaggaac gcaggggctg gaataggagg gaaaggaggt
       61 ggctccagga gagagcgaga gagggagcgc tcgcatcggg gctcagtggc accagaccta
      121 aaaagaaatc taggcaaggc tccggcacag tccacgtggt ggaaagataa ggaattggaa
      181 cagaaccaac agtcgtttct acagcctctc aatggatcct gtcaggccgt tgttcagggg
      241 gcccacccca gtccacccat ctcagtgtgt gcggatgcca ggctgttggc ctcaagctcc
      301 tagacctttg gaaccagctt ggggtagggc aggacctgca ggcagaggcc ttgtgtttag
      361 aaaaccagaa gactccagcc caccactcca gccagtacaa aaggattctg taggtttggt
      421 gtccatgttc cgtggcatgg gtcttgacac agcattccgg cctccttcaa aacgagaagt
      481 gcctccttta ggcagaggag ttctaggtcg aggcttgtct gctaacatgg tccgcaagga
      541 cagagaagaa ccccgttcct ctttgcctga tccttcggtg ctggcagctg gggacagcaa
      601 actggcagag gcttctgttg gttggagtag aatgctggga agaggtagtt ctgaggtctc
      661 tctgttacca ctgggacgag cagccagtag tataggcaga ggaatggaca aacctcccag
      721 tgcctttggc ctcacagctc gggatccccc acggctgcca cagcctccag ctctgtctcc
      781 aacttcactg cactctgccg atccccctcc ggtcctgact atggaacgaa aggaaaaaga
      841 gcttttggtc aagcaaggat caaaaggaac tcctcagtct ttgggactga acctcatcaa
      901 aatccagtgt cataacgaag cagtttatca gtaccatgtg actttcagcc ccagtgtgga
      961 atgcaaaagc atgaggtttg gcatgttgaa ggaccaccag tctgtcactg gaaacgtcac
     1021 tgcttttgat ggctctattc tttatcttcc tgttaagctt caacaagttg ttgagttaaa
     1081 aagtcagagg aaaactgacg atgccgagat cagtatcaag attcagctga caaagatcct
     1141 ggagccgtgt tctgacctgt gcatcccctt ctacaatgtt gtcttccggc gggtaatgaa
     1201 acttctggat atgaagcttg tggggagaaa cttctatgac cctacaagtg ccatggtact
     1261 gcagcaacac agattgcaga tctggcctgg ctatgcggct agtatccgga ggacagacgg
     1321 gggtctcttc ctgctcgctg atgtctctca taaggtcatt cggaacgact ctgtgctgga
     1381 tgtcatgcat gctatctacc agcagaacaa ggagcacttc caggacgagt gcagcaagct
     1441 tctggttggc agcattgtca tcacgcgcta caacaatcgt acctaccgaa tcgatgatgt
     1501 ggactggaac aagaccccta aagacagctt tgtcatgtcg gacgggaagg aaatcacatt
     1561 cctggaatac tacagcaaaa actatgggat cacagtcaag gaagatgacc agccgctgct
     1621 gatccaccgg cccagtgaga gacagaataa ccatggcatg ttgctgaagg gcgagatcct
     1681 gctgctgccc gagctctcct tcatgacggg gatccctgag aagatgaaga aggacttcag
     1741 ggccatgaag gacttgactc agcagattaa cctgagcccc aagcagcacc acggtgcttt
     1801 ggaatgcctg ctgcagagaa tttcacaaaa cgagacagcc agcaatgagc tgacccgctg
     1861 ggggctcagt ctgcataaag atgtccacaa gattgaaggt cggcttctgc caatggagag
     1921 gatcaactta aggaacactt catttgtcac atcggaggac ctgaactggg ttaaggaagt
     1981 gaccagagat gcttccattc taactattcc catgcatttc tgggcactct tttatccaaa
     2041 gagagcaatg gaccaagcca gagaactggt taacatgttg gaaaagattg ccgggcccat
     2101 tggcatgcgc ataagccccc cagcctgggt tgagctgaag gatgaccgaa tagagaccta
     2161 tatcaggacc attcagtcct tactgggagt tgaggggaag atacaaatgg tcgtttgcat
     2221 catcatgggc acacgtgatg atctctatgg agccatcaag aagctgtgct gcgtgcagtc
     2281 cccagtgccc tcacaggtca tcaatgtccg aaccattggt cagcccacca ggcttcggag
     2341 cgtggctcag aaaattttac ttcagatgaa ctgtaaactg ggtggtgagc tctggggagt
     2401 ggatattccg ctgaaacaac taatggtgat tggaatggat gtgtaccatg accccagcag
     2461 aggcatgcgc tctgtggtcg gcttcgtggc cagcataaat ctcacactca ccaaatggta
     2521 ctcgagggtg gtgttccaga tgccacatca ggagattgtg gacagcctga agctctgcct
     2581 ggtgggttcc ttgaaaaagt attatgaggt gaaccattgt ctcccagaga aaattgtggt
     2641 gtaccgagat ggagtgtctg atggccagct aaagacagtt gccaactacg agatccctca
     2701 gctgcagaag tgttttgaag cctttgataa ctaccacccc aagatggtgg tgtttgtagt
     2761 tcagaagaaa atcagcacca atctgtacct tgctgctcct gatcacttcg taaccccctc
     2821 ccccgggact gtggttgatc ataccataac cagctgtgag tgggtggatt tctaccttct
     2881 tgcccatcat gtgcgacagg gctgtggcat acctacacac tacatctgtg ttctgaacac
     2941 tgcaaatctg agccctgatc acatgcagag gttgactttc aaactatgcc acatgtactg
     3001 gaattggcct ggtaccatcc gagttccagc tccttgcaag tatgcccaca agctagcttt
     3061 cctgtccgga cagattttgc atcatgagcc agccatccag ctgtgtggga acctgttctt
     3121 cctgtaactg ggaacttgga caccggctgc aaggagcaac tggactcagc tcagctcctc
     3181 cttacagaat caacagaaat ggcagtggaa ttttatgttt gcattttctc tttctccatc
     3241 cttgtagaat tagatttctg ttcttctttt aaccctgata tcatagtagg gtgttgtggt
     3301 gcatgccttt tatcccagca cttgggagac tgaagcagga gtatctttag ttcaaggcca
     3361 gcctagacta catggtgagt tctaggctag ccaagattac agagtgagac cctgtctcaa
     3421 aaaacaaaaa caaaccactg tcccctcaaa gcccacaaca aaaccaaagc ctggggtcaa
     3481 ggaagcaagt ttgtaggtag ccgctggctg cccgctgcct tcatggagtg tgtgccgtca
     3541 gcgctgcttc tcctcagccg agcgtggagc ttcggacagg gcagtgatga catgttctta
     3601 gcatgtcaaa tccccctacc aaataagtca agcaaggaaa aaatagcccc caaggcagcc
     3661 tgagcatcag ttcctagagg ttatgcctac agaaccatcc tatttctggt ggcagaagtg
     3721 acatgaagtc attgcagaca tcttaaagga gagttactgt gcagctgtct acatgtgtga
     3781 aagacatgta gaaaaaccag cgtagggtac agtcgggcgt atgtgcccac ctgacccagg
     3841 gctgtgagtc tgacttcccg agagtctggc tagagctgct tttctggtcc ttttggttat
     3901 ttgcaaccat catcagattg ctttcctgca gcccgactga tacacgcatg tgcgcacgca
     3961 cacacttttg ttctttgtga ctaatcttga ataaattcaa ttagaacaca tggaaaggat
     4021 tcagcagacc taggaacatt tggggtggag tgtagttttt ctgcaaaagt ctgtaaatga
     4081 gattacgcaa gagtctcttc cagctgtggg ctggtgttgc ttggaaaatt tcaaaatccc
     4141 aaagtttcag gcttcccaaa gttggcttgg aaaaatgtga tagtctcacc tgagtctaga
     4201 catgtaggaa attttcctag ggcctctggg cttcagtatt tgggggaagc actggttttc
     4261 tgtgttattt ttttccttct gtttcagaat cttcaagttt cttcaggctt cagtgggcca
     4321 tccctttact gggctctaaa agctaatttt acttaacctt ttcaaatgtg tatgtatcta
     4381 tttatgtttt ggtgtggtgg atggatggta ggggactgag cagaaatagt cattttaaat
     4441 aacagtgtgc taggagagcc tcagtgtgaa gtcctgagga gcagcggggg ctgtgggagt
     4501 cagtgtcagc cctcactcag acaggccaag cctgggctcg aagacaacat tgtccaggga
     4561 agcctttagt ttgctatagc acccagaggt tggcgaggaa taagggttag ggtcctcaaa
     4621 tacacccatg gcttttggag tctatgacca aggccaggct gagacctgaa atgtaaaagc
     4681 catagaatta aacagaacag actgaaaaac gagtcttaag ttccactttc tggatttctc
     4741 tggaagtctt ttgaatttgt ctgtagaagt tgctgttcct agcactcctt ttctctttag
     4801 gtagaacaga tacttgacca taatgccaga atgtactttc tctgccttgg tttttctatg
     4861 ccttgttttt cagtttcagg gccaaacaat tgggccctgt ggtgtaaaat aaaaacaatg
     4921 tatgtgtata
//
