LOCUS       NM_021311               3944 bp    mRNA    linear   ROD 16-OCT-2024
DEFINITION  Mus musculus piwi-like RNA-mediated gene silencing 1 (Piwil1),
            mRNA.
ACCESSION   NM_021311 XM_974147
VERSION     NM_021311.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Xie,S., Qin,R., Zeng,W., Li,J. and Lai,Y.
  TITLE     Pseudopregnant mice generated from Piwil1 deficiency sterile mice
  JOURNAL   PLoS One 19 (5), e0296414 (2024)
   PUBMED   38771805
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Wei,H., Gao,J., Lin,D.H., Geng,R., Liao,J., Huang,T.Y., Shang,G.,
            Jing,J., Fan,Z.W., Pan,D., Yin,Z.Q., Li,T., Liu,X., Zhao,S.,
            Chen,C., Li,J., Wang,X., Ding,D. and Liu,M.F.
  TITLE     piRNA loading triggers MIWI translocation from the
            intermitochondrial cement to chromatoid body during mouse
            spermatogenesis
  JOURNAL   Nat Commun 15 (1), 2343 (2024)
   PUBMED   38491008
  REMARK    GeneRIF: piRNA loading triggers MIWI translocation from the
            intermitochondrial cement to chromatoid body during mouse
            spermatogenesis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3944)
  AUTHORS   Li,Z., Liu,X., Zhang,Y., Li,Y., Zhou,L. and Yuan,S.
  TITLE     FBXO24 modulates mRNA alternative splicing and MIWI degradation and
            is required for normal sperm formation and male fertility
  JOURNAL   Elife 12, 91666 (2024)
   PUBMED   38470475
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3944)
  AUTHORS   Dali,O., D'Cruz,S., Legoff,L., Diba Lahmidi,M., Heitz,C.,
            Merret,P.E., Kernanec,P.Y., Pakdel,F. and Smagulova,F.
  TITLE     Transgenerational epigenetic effects imposed by neonicotinoid
            thiacloprid exposure
  JOURNAL   Life Sci Alliance 7 (2), e202302237 (2023)
   PUBMED   37973188
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3944)
  AUTHORS   Wei,C., Jing,J., Yan,X., Mann,J.M., Geng,R., Xie,H., Demireva,E.Y.,
            Hess,R.A., Ding,D. and Chen,C.
  TITLE     MIWI N-terminal RG motif promotes efficient pachytene piRNA
            production and spermatogenesis independent of LINE1 transposon
            silencing
  JOURNAL   PLoS Genet 19 (11), e1011031 (2023)
   PUBMED   37956204
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3944)
  AUTHORS   Kuramochi-Miyagawa,S., Kimura,T., Ijiri,T.W., Isobe,T., Asada,N.,
            Fujita,Y., Ikawa,M., Iwai,N., Okabe,M., Deng,W., Lin,H., Matsuda,Y.
            and Nakano,T.
  TITLE     Mili, a mammalian member of piwi family gene, is essential for
            spermatogenesis
  JOURNAL   Development 131 (4), 839-849 (2004)
   PUBMED   14736746
REFERENCE   7  (bases 1 to 3944)
  AUTHORS   Tan,C.H., Lee,T.C., Weeraratne,S.D., Korzh,V., Lim,T.M. and Gong,Z.
  TITLE     Ziwi, the zebrafish homologue of the Drosophila piwi:
            co-localization with vasa at the embryonic genital ridge and
            gonad-specific expression in the adults
  JOURNAL   Mech Dev 119 Suppl 1, S221-S224 (2002)
   PUBMED   14516689
REFERENCE   8  (bases 1 to 3944)
  AUTHORS   Qiao,D., Zeeman,A.M., Deng,W., Looijenga,L.H. and Lin,H.
  TITLE     Molecular characterization of hiwi, a human member of the piwi gene
            family whose overexpression is correlated to seminomas
  JOURNAL   Oncogene 21 (25), 3988-3999 (2002)
   PUBMED   12037681
REFERENCE   9  (bases 1 to 3944)
  AUTHORS   Deng,W. and Lin,H.
  TITLE     miwi, a murine homolog of piwi, encodes a cytoplasmic protein
            essential for spermatogenesis
  JOURNAL   Dev Cell 2 (6), 819-830 (2002)
   PUBMED   12062093
REFERENCE   10 (bases 1 to 3944)
  AUTHORS   Kuramochi-Miyagawa,S., Kimura,T., Yomogida,K., Kuroiwa,A.,
            Tadokoro,Y., Fujita,Y., Sato,M., Matsuda,Y. and Nakano,T.
  TITLE     Two mouse piwi-related genes: miwi and mili
  JOURNAL   Mech Dev 108 (1-2), 121-133 (2001)
   PUBMED   11578866
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC111089.27.
            
            On Nov 17, 2006 this sequence version replaced NM_021311.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF438405.1, AB032604.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-109               AC111089.27        141956-142064
            110-187             AC111089.27        143159-143236
            188-268             AC111089.27        144754-144834
            269-383             AC111089.27        145144-145258
            384-509             AC111089.27        146634-146759
            510-724             AC111089.27        147202-147416
            725-846             AC111089.27        147703-147824
            847-927             AC111089.27        148937-149017
            928-1125            AC111089.27        149490-149687
            1126-1237           AC111089.27        149984-150095
            1238-1364           AC111089.27        151114-151240
            1365-1482           AC111089.27        151358-151475
            1483-1597           AC111089.27        151927-152041
            1598-1785           AC111089.27        153002-153189
            1786-1858           AC111089.27        153545-153617
            1859-2059           AC111089.27        155556-155756
            2060-2163           AC111089.27        155908-156011
            2164-2234           AC111089.27        156541-156611
            2235-2388           AC111089.27        156692-156845
            2389-2514           AC111089.27        158293-158418
            2515-2662           AC111089.27        159651-159798
            2663-3944           AC111089.27        159903-161184
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 67.86 cM"
     gene            1..3944
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /note="piwi-like RNA-mediated gene silencing 1"
                     /db_xref="GeneID:57749"
                     /db_xref="MGI:MGI:1928897"
     exon            1..109
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            110..187
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            188..268
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..2779
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /note="piwi-like homolog 1"
                     /codon_start=1
                     /product="piwi-like protein 1"
                     /protein_id="NP_067286.1"
                     /db_xref="CCDS:CCDS19690.1"
                     /db_xref="GeneID:57749"
                     /db_xref="MGI:MGI:1928897"
                     /translation="MTGRARARARGRARGQETVQHVGAAASQQPGYIPPRPQQSPTEG
                     DLVGRGRQRGMVVGATSKSQELQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVK
                     ESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGR
                     CHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFR
                     RLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLR
                     SETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFK
                     KADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTG
                     LTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNL
                     LSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEA
                     ANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRK
                     DKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPL
                     KLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQA
                     ALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIV
                     VKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVI
                     YDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLS
                     NRLYYL"
     exon            269..383
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            384..509
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            510..724
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            725..846
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            847..927
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1125
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1237
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1364
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1482
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1597
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1785
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..1858
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..2059
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2163
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2234
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            2235..2388
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2514
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            2515..2662
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     exon            2663..3944
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3922..3927
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /note="hexamer: AATAAA"
     polyA_site      3944
                     /gene="Piwil1"
                     /gene_synonym="MIWI"
                     /note="major polyA site"
ORIGIN      
        1 agaagcctgc tgcagggggt ggcgtgagga gcctcaggga ctgcggacct aagcgtgcta
       61 agcacggggc caggatagga ctggacgctg ctggaccacg acgatcaggg agtgaccccc
      121 accagcatcc agcctgcggc agttgtagga ctggaaggag gccaagctga acacctgagc
      181 tgactggaaa atgactggcc gagcccgagc tcgggcccgc ggcagggcac gaggtcagga
      241 gacggtgcag catgttgggg ctgctgcgag ccagcaacct gggtacatcc caccgagacc
      301 tcaacagtcc cccacagagg gggacttggt tggccgagga cgacagaggg ggatggtagt
      361 cggagccaca tccaagtcac aagaactgca gatctcagct gggtttcagg agctgtcact
      421 ggcagagaga ggagggcgtc gccgagactt ccatgacctt ggtgtgaaca ccagacagaa
      481 ccttgaccat gtcaaagagt caaagacagg ctcctctggc atcattgtga agctgagcac
      541 caaccacttc cggctgacct cgcgcccaca gtgggccctg tatcagtacc acatcgacta
      601 caatcccctg atggaggccc gaaggcttcg ctccgcactg ctcttccagc atgaagacct
      661 cattggaagg tgtcatgctt tcgatgggac aatattgttt ttacctaaga gactacagca
      721 caaggtcaca gaagtattca gtcagactcg gaatggggaa cacgtgagga tcaccatcac
      781 cctgaccaac gagctgccgc ccacctcgcc cacctgcctg cagttctata acatcatctt
      841 caggaggctc ttgaaaatca tgaatttgca acaaattgga cggaattatt acaatccaag
      901 tgacccgatt gatattccaa accacaggtt ggtgatctgg cccggcttca ccacctccat
      961 ccttcagtat gagaacaaca tcatgctctg cacagacgtc agccacaagg tgctccgcag
     1021 cgagactgtc ctagacttca tgttcaatct ataccagcag acagaggagc acaagttcca
     1081 ggagcaagtg tcgaaggagc tcataggcct catcgttctc accaagtaca ataacaagac
     1141 ctaccgggtg gatgacattg actgggacca gaatccaaag agcaccttca agaaggcgga
     1201 tggctcggag gtcagcttcc tggagtacta caggaagcaa tacaaccagg agatcacgga
     1261 cctgaagcag ccggtgctgg tgagccaacc caagcggagg agaggccccg gcggcaccct
     1321 gcctggccca gctatgctca tccctgaact ctgctatctc acaggcctga ctgataaaat
     1381 gcgcaatgat ttcaatgtga tgaaggacct ggcagtgcac acgcggctga cccctgagca
     1441 gcggcagcgg gaggtgggcc gcctcatcga ctacatccac aaggatgaca atgtgcagag
     1501 agagcttcga gactggggcc tgagcttcga ctcaaacttg ctgtccttct ctggaagaat
     1561 cttacaatct gagaagatcc accagggcgg aaagacgttt gattacaacc cacaatttgc
     1621 agactggtcc aaagaaacaa gaggcgcgcc gctgatcagc gtgaagccat tggataactg
     1681 gctgctgatc tatacccgca ggaattatga agcagccaac tcactgatac agaacctgtt
     1741 caaagtgact ccagccatgg gcatccagat gaaaaaggca atcatgatcg aggtggatga
     1801 cagaacagaa gcttatctga gagccttgca gcagaaggtg acgtcagaca ctcagatagt
     1861 tgtctgtctc ttgtcaagta atcggaagga caaatatgat gccatcaaga agtacttgtg
     1921 tacagactgc cccaccccaa gtcagtgtgt ggtggcccgg accctgggca agcagcaaac
     1981 agtcatggcc attgccacca agatcgccct gcagatgaac tgcaagatgg gaggcgagct
     2041 ctggcgggtg gacatgcccc tgaaactggc aatgatcgtg ggcatcgact gttaccatga
     2101 caccacagct gggcggaggt ccatcgcagg attcgtcgcc agcatcaatg aagggatgac
     2161 ccgctggttc tcccgctgcg tctttcagga ccgcgggcag gagctggtgg atggtctcaa
     2221 ggtgtgcttg caagctgctc tgagggcttg gagtggctgc aatgaataca tgcccagccg
     2281 tgtcatcgtg taccgagacg gtgtggggga cgggcagctg aagaccctgg tcaattatga
     2341 ggtcccacag ttcctagatt gcctcaagtc agtcgggaga ggttacaacc caagactgac
     2401 tgtaatcgtg gtgaagaagc gtgtcaatgc caggtttttt gctcagtctg ggggaagact
     2461 tcagaaccct cttccaggga cagtcatcga tgtggaagtc accagaccag agtggtatga
     2521 ctttttcatc gtgagccagg cagtgagaag cgggagtgtg tccccaacac actacaatgt
     2581 catctatgac agcagtggcc tgaagcccga ccacatccag cggctgacat acaagctctg
     2641 ccacgtgtac tataattggc ctggagtcat ccgagtccct gcaccttgcc agtatgcaca
     2701 caagctggcc ttcctcgtgg gccagagcat ccacagagag ccaaacctct ccctgtccaa
     2761 ccgcctctac tacctctaac cagcagcgag ggccggcatg ctggtttcct tcagagctct
     2821 gccaatcctt cagttctatt tttcatagga tgaggttcta gcgggatgtt gggggtaggg
     2881 gttgggggcc gctgttttgt tttgttttgt ttttagttgt tggtgtgtag cggctataga
     2941 gttaactaag gcttatgttt gctttgtttc tcatggtgtg ttgtgacagt ttgcttggca
     3001 gagtggtggc aagggccgtg gtaccacaga tagactttcc taaagcagcc gtgttggcag
     3061 gttcttggag gtgctcctgg gagcacctcc tattgtacgc tgctctctct ctctctctct
     3121 ctctctctct ctctctctct ctctctctct gcatgtttga atttaaaaga ggaaaggaca
     3181 caaggcaaga atgcccagag catcctagat gggtgctata tgggactctg acacctcagg
     3241 ttcttaaaga taaaaaggcc agaggtacct ttctgtaatt cttttaagag ttaggtcata
     3301 aggaatgaaa agagttactt ttctccctcc atgaaagtgt tttttaagac tgacaaggcc
     3361 ggtggtggag tgagtgttgt tgttttgttt cgcaattttc ttaccaagtg aaattactga
     3421 ccataatttt atttaatagt ttttagtttt tgaaagcctg tgttttcttt gcttttaaac
     3481 tacaggcgac cactctggga taggattcca ctcccaaaag taatgtgttg ggtgtggtgg
     3541 cacacgcctg tgatctcagg actcaggagg tgaagccagg ggaaccccaa gttcccggtc
     3601 agcctaggct acatagtgag actttgtctt aacaaacaga aaaaaagaaa aagaaagaaa
     3661 aaaaaaagaa agaaaaaaaa agaaagaaaa atggagcagt atgagttatg ggtgccagtt
     3721 tctgcaggag actttgggag taagagctgc ctctgttcct aagaggctgc aaggctgcgg
     3781 ctggggggcc agttcaggtg ccaggtgagg tacggccaca tctatttgcc tggctctttg
     3841 acaaagagag cagcttagag tggctagagt gtgatgagtt ttacacaatg gcgatgtgtg
     3901 tgtgtgcccc tggcacagct caataaagtt tctgtctcac ttta
//
