LOCUS       NM_145131               3547 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_145131
VERSION     NM_145131.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3547)
  AUTHORS   Du,F., Yu,Q., Yan,S., Zhang,Z., Vangavaragu,J.R., Chen,D., Yan,S.F.
            and Yan,S.S.
  TITLE     Gain of PITRM1 peptidase in cortical neurons affords protection of
            mitochondrial and synaptic function in an advanced age mouse model
            of Alzheimer's disease
  JOURNAL   Aging Cell 20 (5), e13368 (2021)
   PUBMED   33951271
REFERENCE   4  (bases 1 to 3547)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3547)
  AUTHORS   Perez-Garcia,V., Fineberg,E., Wilson,R., Murray,A., Mazzeo,C.I.,
            Tudor,C., Sienerth,A., White,J.K., Tuck,E., Ryder,E.J., Gleeson,D.,
            Siragher,E., Wardle-Jones,H., Staudt,N., Wali,N., Collins,J.,
            Geyer,S., Busch-Nentwich,E.M., Galli,A., Smith,J.C., Robertson,E.,
            Adams,D.J., Weninger,W.J., Mohun,T. and Hemberger,M.
  TITLE     Placentation defects are highly prevalent in embryonic lethal mouse
            mutants
  JOURNAL   Nature 555 (7697), 463-468 (2018)
   PUBMED   29539633
REFERENCE   6  (bases 1 to 3547)
  AUTHORS   Town,L., McGlinn,E., Fiorenza,S., Metzis,V., Butterfield,N.C.,
            Richman,J.M. and Wicking,C.
  TITLE     The metalloendopeptidase gene Pitrm1 is regulated by hedgehog
            signaling in the developing mouse limb and is expressed in muscle
            progenitors
  JOURNAL   Dev Dyn 238 (12), 3175-3184 (2009)
   PUBMED   19877269
  REMARK    GeneRIF: Pitrm1 is expressed in Pax3-expressing myoblast
            progenitors in the limb, the dermomyotome, and developing muscles
            of the face and torso.
REFERENCE   7  (bases 1 to 3547)
  AUTHORS   Chen,H.W., Chen,J.J., Yu,S.L., Li,H.N., Yang,P.C., Su,C.M.,
            Au,H.K., Chang,C.W., Chien,L.W., Chen,C.S. and Tzeng,C.R.
  TITLE     Transcriptome analysis in blastocyst hatching by cDNA microarray
  JOURNAL   Hum Reprod 20 (9), 2492-2501 (2005)
   PUBMED   15919778
REFERENCE   8  (bases 1 to 3547)
  AUTHORS   Ehrich,T.H., Hrbek,T., Kenney-Hunt,J.P., Pletscher,L.S., Wang,B.,
            Semenkovich,C.F. and Cheverud,J.M.
  TITLE     Fine-mapping gene-by-diet interactions on chromosome 13 in a LG/J x
            SM/J murine model of obesity
  JOURNAL   Diabetes 54 (6), 1863-1872 (2005)
   PUBMED   15919810
REFERENCE   9  (bases 1 to 3547)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   10 (bases 1 to 3547)
  AUTHORS   Suemizu,H., Aiba,K., Yoshikawa,T., Sharov,A.A., Shimozawa,N.,
            Tamaoki,N. and Ko,M.S.
  TITLE     Expression profiling of placentomegaly associated with nuclear
            transplantation of mouse ES cells
  JOURNAL   Dev Biol 253 (1), 36-53 (2003)
   PUBMED   12490196
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF513714.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF513714.1, AK141277.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 3.25 cM"
     gene            1..3547
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /note="pitrilysin metallepetidase 1"
                     /db_xref="GeneID:69617"
                     /db_xref="MGI:MGI:1916867"
     exon            1..73
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     CDS             18..3128
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; pitrilysin metalloprotease 1; presequence
                     protease, mitochondrial; pitrilysin metalloproteinase 1;
                     nuclear transplantation upregulated protein 1"
                     /codon_start=1
                     /product="presequence protease, mitochondrial isoform 1
                     precursor"
                     /protein_id="NP_660113.1"
                     /db_xref="CCDS:CCDS36585.1"
                     /db_xref="GeneID:69617"
                     /db_xref="MGI:MGI:1916867"
                     /translation="MWRFSGRRGLCAVQRLSCGRVHHRVWREKSDQACERALQYKVGE
                     KIHGFTVNQVTPVPELFLTAVKLSHDNTGARYLHLAREDKNNLFSVQFRTTPMDSTGV
                     PHVLEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAMTASDYTIYPFSTQNPKDFQNLL
                     SVYLDATFFPCLRELDFWQEGWRLEHENPRDPQTPLIFKGVVFNEMKGAFTDNERIFS
                     QHLQNKLLPDHTYSVVSGGDPLCIPELTWEQLKQFHATHYHPSNARFFTYGNFQLEGH
                     LKQIHEEALSKFQRLEQSTAVPAQPHWDKPREFHITCGPDSLATETAKQTTVSVSFLL
                     PDITDTFEAFTLSLLSSLLIAGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFSVG
                     LQGIAEKDVKTVRELVDRTIEEVIEKGFEDDRIEALLHKIEIQTKHQSASFGLTLTSY
                     IASCWNHDGDPVELLQIGSQLTRFRKCLKENPKFLQEKVEQYFKNNQHKLTLSMKPDD
                     KYYEKQTQMETEKLEQKVNSLSPADKQQIYEKGLELQTQQSKHQDASCLPALKVSDIE
                     PSMPFTKLDIGLAAGDIPVQYCPQPTNGMVYFRAFSSLNTLPEDLRPIVPLFCSVLTK
                     LGCGILNYREQAQQIELKTGGMSVTPHVLPDDSQLDTYEQGVLFSSLCLERNLPDMMH
                     LWSEIFNNPCFEEEEHFKVLVKMTAQELSNGISDSGHLYAALRASKTLTPSGDLQETF
                     SGMDQVKVMKRIAEMTDIKPILRKLPRIKKYLLNCDNMRCSVNATPQQMPQAEKEVEN
                     FLRNVGRSKKERKPVRPHIVEKPTPSGPSGAAHVSGSQIVRKLVTDPTFKPCQMKTHF
                     VLPFPVNYIGECVRTVPYADPDHASLKILARLMTAKFLHTEIREKGGAYGGGAKLTHS
                     GIFTLYSYRDPNSIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDSPVAPSDKG
                     MDHFLYGLSDEMKQAYREQLFAVNHDKLTSVSHKYLGIGKSTHGLAILGPENSKIAKD
                     PSWIIK"
     exon            74..176
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            177..283
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            284..435
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            436..550
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            551..647
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            648..808
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            809..935
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1024
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1153
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1267
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1364
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1499
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1638
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1755
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1888
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..2009
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2086
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2252
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2353
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            2354..2474
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            2475..2546
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            2547..2659
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            2660..2785
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            2786..2931
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            2932..3034
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     exon            3035..3543
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3406..3411
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /note="hexamer: TATAAA"
     polyA_site      3423
                     /gene="Pitrm1"
                     /gene_synonym="2310012C15Rik; mKIAA1104; MP-1; MP1; Ntup1;
                     PreP"
                     /note="major polyA site"
ORIGIN      
        1 cacgctggtc acatgccatg tggcgcttca gcggtcggcg gggactctgc gctgtacagc
       61 ggctgagctg cggcagggta caccacagag tatggaggga gaagagtgac caagcctgtg
      121 aacgagctct acagtataaa gtgggagaga aaatccacgg gttcactgta aaccaggtca
      181 ctcctgtccc cgagctgttc ctgacagccg tgaagctcag ccatgacaac acgggagcca
      241 gatacctgca cctggcaagg gaagacaaga acaacttatt cagtgtgcag ttccgcacaa
      301 ccccaatgga tagcactggg gtcccacatg ttctcgagca tacggtcctg tgcggctctc
      361 agaagtaccc gtgcagagat cctttcttca aaatgctcaa caggtcactg tccacattta
      421 tgaatgccat gacagccagc gattacacga tatatccgtt ttccactcaa aatcccaaag
      481 attttcagaa cctcctctcc gtgtatttgg atgcaacttt cttcccctgc ttgagggaac
      541 tggacttctg gcaggaagga tggcgtctgg agcatgagaa tccccgagac cctcagacgc
      601 ccttgatctt taagggggtc gtcttcaacg agatgaaagg ggcatttaca gacaatgaga
      661 ggatattctc ccagcacctg cagaacaagc tgcttcctga ccacacctac tccgtggttt
      721 ctggagggga cccactgtgc atcccggagc tcacgtggga acagctgaaa cagttccacg
      781 ctactcatta tcacccaagc aatgccaggt tcttcactta tggcaatttt cagctggaag
      841 gacacctgaa acaaattcac gaagaagccc tgagtaaatt ccagagattg gagcagagta
      901 cagcagtgcc tgcccagccg cactgggata agcctaggga attccatata acatgtggcc
      961 cagattcact agctacggag actgccaagc agacaactgt cagcgttagc ttcctcttac
     1021 cggatatcac tgacacattt gaagccttca ccttgagcct tctgtcctcc ctcctgattg
     1081 ctggacccaa ctcccccttc tacaaagctt tgatcgagtc tggactcggc acagactttt
     1141 ctcctgatgt tggatataat ggctatacac gggaggctta cttcagtgtc gggctccaag
     1201 ggatcgcaga gaaagatgtc aagacggtca gagagctcgt agacaggaca atcgaagaag
     1261 ttatagagaa aggatttgaa gatgatcgga ttgaagctct gcttcataaa atcgaaattc
     1321 aaacgaagca tcagtcagcc agctttggcc tgaccctgac gtcatatata gcttcttgct
     1381 ggaaccatga tggggaccct gtggagctcc tgcagattgg aagtcagctg actagattta
     1441 ggaagtgcct taaggaaaat ccaaaatttt tacaagaaaa agtagaacaa tattttaaga
     1501 acaatcagca caagctgact ttatccatga agccagacga caagtattat gaaaagcaaa
     1561 ctcagatgga gacagaaaag ctggagcaaa aggtgaattc tctctccccg gcggacaagc
     1621 agcagatcta cgagaaaggt ttagaactac agacgcagca aagtaaacat caagacgcct
     1681 cctgcctccc agcattgaaa gtctcggaca ttgagccctc catgcctttc accaagcttg
     1741 acatcggcct tgcagctgga gacatccctg tgcagtactg cccacagccc accaacggca
     1801 tggtgtattt ccgagccttt tccagtttaa acacgctgcc ggaggacctg aggcccattg
     1861 tgcctctctt ttgcagcgtg ctgaccaagc tgggttgtgg catccttaac tacagagagc
     1921 aagcccaaca gattgagctc aagacaggag gcatgagtgt cacgccccat gtgctccctg
     1981 acgactcaca gctggatacc tacgagcagg gtgtgttatt ttcatctctc tgcctggagc
     2041 ggaacctgcc agacatgatg catctgtgga gcgaaatatt taacaatcca tgctttgaag
     2101 aagaagaaca cttcaaagtg ttggtgaaga tgaccgctca ggagctctcc aatggaattt
     2161 cagactcggg gcatctctat gcagccctca gagcaagcaa gacactgaca ccttcagggg
     2221 acttgcagga gaccttcagt gggatggatc aggtgaaggt gatgaaaaga attgcagaga
     2281 tgacagacat caagcccatc ctgagaaaac tgccccggat caagaagtat ctactaaact
     2341 gtgacaacat gagatgctca gtgaatgcca cccctcagca gatgcctcag gcagaaaaag
     2401 aggtggaaaa cttccttaga aatgttggcc gaagcaaaaa ggaacggaag cctgtccgcc
     2461 cgcatattgt cgagaaaccc acacccagtg gccccagtgg agctgcacat gtcagtgggt
     2521 cccagatcgt cagaaaattg gtgacagacc ccaccttcaa accctgccag atgaagacac
     2581 attttgtgct gcccttccct gtgaattaca ttggcgagtg tgtcaggact gtcccgtatg
     2641 ctgatccaga ccatgccagc cttaagatcc ttgcccgtct aatgacagct aaattcttgc
     2701 atacggaaat tcgagagaag gggggtgctt atggtggcgg tgctaaactc acccacagtg
     2761 ggattttcac gctttactct tacagggatc ccaattccat agaaacactc cagtcttttg
     2821 ggaaagctgt agactgggct aagtctggaa agttcacaca gcaggacatt gatgaagcca
     2881 agctgtctgt tttctctact gtggattctc ctgttgctcc atccgataaa ggaatggacc
     2941 acttcttgta tggcctctcc gatgagatga agcaggcata ccgagaacag ctctttgctg
     3001 tcaaccacga caaactgacc tctgtgagcc ataaatacct tggcatcggg aagagcacac
     3061 acggcctggc tatcctcgga ccagagaact caaaaattgc caaagaccca tcatggatca
     3121 taaaataatg agtgccacat atctttgagt atgtgtaaga aaccagaggc tcttaacagc
     3181 tgagcctctg agctaaattt aatgcggatg atcacaagag ttactagttt ctttgttcgg
     3241 aaaatcagtt agccatataa aaaccaacca aaggtgttta ttgactggca aaaactctga
     3301 aggaaattct gagaccatga agaaatcatt aatcatgcat taaatgacag cagtgagcaa
     3361 attaagcctc tgaaacatta ctaagcctag aatatgtatt ttaaatataa aagccaactc
     3421 caacctgtct gagttttact cattattttc aaacaataat ttacgacaat aatgtttaga
     3481 ccttcactta agaaaaatgg ctaaatcaaa tcaaaatcta aatcaaaaaa ataaaaaaaa
     3541 aaaaaaa
//
