LOCUS       NM_019640               2847 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus phosphatidylinositol transfer protein, beta (Pitpnb),
            transcript variant 2, mRNA.
ACCESSION   NM_019640
VERSION     NM_019640.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Xie,Z. and Bankaitis,V.A.
  TITLE     Phosphatidylinositol transfer protein/planar cell polarity axis
            regulates neocortical morphogenesis by supporting interkinetic
            nuclear migration
  JOURNAL   Cell Rep 39 (9), 110869 (2022)
   PUBMED   35649377
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Capitano,M., Zhao,L., Cooper,S., Thorsheim,C., Suzuki,A., Huang,X.,
            Dent,A.L., Marks,M.S., Abrams,C.S. and Broxmeyer,H.E.
  TITLE     Phosphatidylinositol transfer proteins regulate megakaryocyte
            TGF-beta1 secretion and hematopoiesis in mice
  JOURNAL   Blood 132 (10), 1027-1038 (2018)
   PUBMED   30042096
  REMARK    GeneRIF: these data show that ablating PITPs in MKs indirectly
            dysregulates hematopoiesis in the BM by disrupting alpha-granule
            physiology and secretion of TGF-beta1.
REFERENCE   3  (bases 1 to 2847)
  AUTHORS   Xie,Z., Hur,S.K., Zhao,L., Abrams,C.S. and Bankaitis,V.A.
  TITLE     A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution
            and Cell Polarity during Neurogenesis
  JOURNAL   Dev Cell 44 (6), 725-740 (2018)
   PUBMED   29587143
REFERENCE   4  (bases 1 to 2847)
  AUTHORS   Dina,C., Bouatia-Naji,N., Tucker,N., Delling,F.N., Toomer,K.,
            Durst,R., Perrocheau,M., Fernandez-Friera,L., Solis,J., Le
            Tourneau,T., Chen,M.H., Probst,V., Bosse,Y., Pibarot,P.,
            Zelenika,D., Lathrop,M., Hercberg,S., Roussel,R., Benjamin,E.J.,
            Bonnet,F., Lo,S.H., Dolmatova,E., Simonet,F., Lecointe,S.,
            Kyndt,F., Redon,R., Le Marec,H., Froguel,P., Ellinor,P.T.,
            Vasan,R.S., Bruneval,P., Markwald,R.R., Norris,R.A., Milan,D.J.,
            Slaugenhaupt,S.A., Levine,R.A., Schott,J.J., Hagege,A.A. and
            Jeunemaitre,X.
  CONSRTM   PROMESA investigators; MVP-France; Leducq Transatlantic MITRAL
            Network
  TITLE     Genetic association analyses highlight biological pathways
            underlying mitral valve prolapse
  JOURNAL   Nat Genet 47 (10), 1206-1211 (2015)
   PUBMED   26301497
REFERENCE   5  (bases 1 to 2847)
  AUTHORS   Schenning,M., Goedhart,J., Gadella,T.W. Jr., Avram,D., Wirtz,K.W.
            and Snoek,G.T.
  TITLE     The anti-apoptotic activity associated with phosphatidylinositol
            transfer protein alpha activates the MAPK and Akt/PKB pathway
  JOURNAL   Biochim Biophys Acta 1783 (10), 1700-1706 (2008)
   PUBMED   18501717
REFERENCE   6  (bases 1 to 2847)
  AUTHORS   Routt,S.M. and Bankaitis,V.A.
  TITLE     Biological functions of phosphatidylinositol transfer proteins
  JOURNAL   Biochem Cell Biol 82 (1), 254-262 (2004)
   PUBMED   15052341
  REMARK    GeneRIF: The PITPbeta deficiency results in catastrophic failure
            early in murine embryonic development, and the PITPbeta is an
            essential housekeeping PIPT in murine cells.
            Review article
REFERENCE   7  (bases 1 to 2847)
  AUTHORS   van Tiel,C.M., Westerman,J., Paasman,M.A., Hoebens,M.M., Wirtz,K.W.
            and Snoek,G.T.
  TITLE     The Golgi localization of phosphatidylinositol transfer protein
            beta requires the protein kinase C-dependent phosphorylation of
            serine 262 and is essential for maintaining plasma membrane
            sphingomyelin levels
  JOURNAL   J Biol Chem 277 (25), 22447-22452 (2002)
   PUBMED   11953429
  REMARK    GeneRIF: The Golgi localization of phosphatidylinositol transfer
            protein beta requires the protein kinase C-dependent
            phosphorylation of serine
REFERENCE   8  (bases 1 to 2847)
  AUTHORS   Alb,J.G. Jr., Phillips,S.E., Rostand,K., Cui,X., Pinxteren,J.,
            Cotlin,L., Manning,T., Guo,S., York,J.D., Sontheimer,H.,
            Collawn,J.F. and Bankaitis,V.A.
  TITLE     Genetic ablation of phosphatidylinositol transfer protein function
            in murine embryonic stem cells
  JOURNAL   Mol Biol Cell 13 (3), 739-754 (2002)
   PUBMED   11907258
  REMARK    GeneRIF: Data suggest that phosphatidylinositol transfer protein
            (PITP) beta is an essential housekeeping PITP in murine cells,
            whereas PITP alpha plays a far more specialized function in
            mammals.
REFERENCE   9  (bases 1 to 2847)
  AUTHORS   Yoder,M.D., Thomas,L.M., Tremblay,J.M., Oliver,R.L., Yarbrough,L.R.
            and Helmkamp,G.M. Jr.
  TITLE     Structure of a multifunctional protein. Mammalian
            phosphatidylinositol transfer protein complexed with
            phosphatidylcholine
  JOURNAL   J Biol Chem 276 (12), 9246-9252 (2001)
   PUBMED   11104777
REFERENCE   10 (bases 1 to 2847)
  AUTHORS   de Vries,K.J., Heinrichs,A.A., Cunningham,E., Brunink,F.,
            Westerman,J., Somerharju,P.J., Cockcroft,S., Wirtz,K.W. and
            Snoek,G.T.
  TITLE     An isoform of the phosphatidylinositol-transfer protein transfers
            sphingomyelin and is associated with the Golgi system
  JOURNAL   Biochem J 310 (Pt 2) (Pt 2), 643-649 (1995)
   PUBMED   7654206
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC122226.3, BY205337.1,
            AK028235.1 and BC034676.1.
            
            On Aug 29, 2014 this sequence version replaced NM_019640.4.
            
            Summary: This gene encodes a member of the phosphatidylinositol
            transfer protein family. The encoded protein catalyzes the transfer
            of phospholipids (phosphatidylinositol and phosphatidylcholine)
            between membranes. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Aug 2014].
            
            Transcript Variant: This variant (2) differs in the 3' UTR and 3'
            coding region compared to variant 1. The encoded isoform (2) has a
            distinct and shorter C-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028235.1, U46934.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AC122226.3         75340-75373
            35-66               BY205337.1         2-33
            67-2265             AK028235.1         2-2200
            2266-2847           BC034676.1         2071-2652
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 53.79 cM"
     gene            1..2847
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /note="phosphatidylinositol transfer protein, beta"
                     /db_xref="GeneID:56305"
                     /db_xref="MGI:MGI:1927542"
     exon            1..137
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..933
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /note="isoform 2 is encoded by transcript variant 2;
                     phosphotidylinositol transfer protein, beta; ptdInsTP
                     beta; ptdIns transfer protein beta; Pitp beta"
                     /codon_start=1
                     /product="phosphatidylinositol transfer protein beta
                     isoform isoform 2"
                     /protein_id="NP_062614.1"
                     /db_xref="CCDS:CCDS39213.1"
                     /db_xref="GeneID:56305"
                     /db_xref="MGI:MGI:1927542"
                     /translation="MVLIKEFRVVLPCSVQEYQVGQLYSVAEASKNETGGGEGIEVLK
                     NEPYENDGEKGQYTHKIYHLKSKVPAFVRMIAPEGSLVFHEKAWNAYPYCRTIVTNEY
                     MKDDFFIKIETWHKPDLGTLENVHGLDPNTWKTVEIVHIDIADRSQVEPADYKADEDP
                     ALFHSVKTKRGPLGPNWKKELANTPDCPRMCAYKLVTIKFKWWGLQSKVENFIQKQEK
                     RIFTNLHRQLFCWIDKWIDLTMEDIRRMEDETQKELETMRKKGSVRGTSAADA"
     exon            138..168
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            169..314
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            315..406
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            407..414
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            415..489
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            490..573
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            574..651
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            652..762
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            763..885
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            886..970
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            971..2827
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1885..1890
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /note="hexamer: AATAAA"
     polyA_site      1902
                     /gene="Pitpnb"
                     /gene_synonym="PI-TP-beta"
                     /note="major polyA site"
ORIGIN      
        1 ggcgcggacg cgcaggcgct cccggggctc ggacgattgg gaggcggggc tacgcttgcg
       61 tgttgcgagg cccggcggtg gcggctggcg gcggcggctg tgaggggttc ccggaagatg
      121 gtgctgatta aggaattccg tgtggttttg ccgtgttctg ttcaagagta tcaggttgga
      181 cagctttact ctgttgcaga agcgagtaag aatgaaactg gtggtggaga aggaatcgaa
      241 gtcctgaaga atgaacctta tgaaaacgat ggcgagaagg gacagtacac acacaaaatc
      301 taccacctga agagcaaagt tcctgcattt gtgaggatga ttgctcccga gggctccctg
      361 gtgtttcatg agaaagcctg gaatgcctac ccctactgca gaacaattgt aacgaatgaa
      421 tacatgaaag atgacttctt catcaaaatt gaaacatggc ataaacctga cttgggaaca
      481 ttagaaaatg ttcacggttt agatcccaac acttggaaaa ctgttgaaat tgtccacata
      541 gacattgcag atcgaagtca agttgaacca gcagactaca aagctgatga agaccctgca
      601 ttattccatt cagtcaagac caagagagga cccctgggac ctaactggaa gaaggagctg
      661 gcaaacaccc ctgactgtcc taggatgtgt gcctataagc tggtgaccat caagttcaag
      721 tggtgggggc tgcagagcaa agtagagaac tttatccaga agcaagaaaa acggatattt
      781 acgaacttac atcgccagct cttttgttgg attgacaagt ggattgacct gacaatggaa
      841 gacattaggc gaatggagga tgagactcag aaagaactag aaacaatgcg taagaagggt
      901 tccgtccgag gcacgtcggc tgctgatgcc tagatgaggc ccctgtaggt cagagacatt
      961 atcagactgt ctacggagtc aaggccaagt gagggggacc agtgcggcct gtgatgactg
     1021 aagccaccag cgtctcagtg ttggacgttt ccagtgtgtg tctgacgatt cgagcacacg
     1081 tgtgcacaca cacggacgga cacacacaga cacacacaca cacatattct cacacactta
     1141 tgcgtgtgtg aatatgtgtg tggatgtctg tagctgtatg taaagcacat atggctacag
     1201 gtctagatac acacatgagt gtatatatat atgtatgtcc atgctagata cacacacgag
     1261 tgtatatata tgtatgtaca tgctagatgc acacatgagt gtatatatat gtatgtacat
     1321 gtaaagatcg aagggagcag ggcgttttct ccaaagtact gcacctgcct tcaacaaaaa
     1381 tactaaagaa aatattttca atatatatac ctggaacaga tattaataat catcaacatg
     1441 atgcccttga tggacaaacg gactgccctg tggtgccccg tggtgctggc tggcgtgctt
     1501 cgtgaatgcc aggtgtggac cagtgtgtac agcctatgat tatgtttctg ttttttcaag
     1561 tcaccacata cacacactta gtctcattcc atcagcaaca actcctacag aaagatttct
     1621 tcaaattttt agatactcca gtgactctgg aatgtaaact ctggatgtat ttataactat
     1681 ttatttctgg gtggccacta tcttccagcc aaatccaaca ttatctgtca agtaaggagt
     1741 caaagctctt tattggtttg cccgagggtt ggggaagtcc ttttttaaaa attgccactt
     1801 tttattggat ttttgtattt tgaatttcaa gtatttttct acatcaaatt tagcaaaaaa
     1861 cagtgatgac ggtttgtgat ttataataaa cactagacaa tattcagact ctggctaagc
     1921 aaacggcgat caagcaatta acctgagtgg tattgcaggc tgtgtggagc acttctgtgg
     1981 agttttgttt ctgaaatggc ttttctggaa ttacagcctg gagactttgt ggatagtctg
     2041 tattgttctc agctcacagg gtgggagagt gatcacgtcc agctcttttt ctgttgtggt
     2101 ccaggccagg aggaaatgaa gagctaggaa cagcttgagt gtggcccagg tctgtcgcca
     2161 tcctgacgcc cagccagacc tgagcgttgc tgccgactct gcttcctcct cattgctcct
     2221 cctcttcctc ctcctcctct tcctcctcct cctcttcctc ctcctcctct gcctctgttt
     2281 tttcctgtaa gagaaaggag cagtagctgg gtaggatttt ctttccttat gacctttctg
     2341 tctgatgctg tttcctcttg ttagccctgg gaccctgacc agaatcacac tgctgaatcc
     2401 ttcaggttac acactgcctt tttaaccccc aacccagagc acagcaccct gccatcagag
     2461 cacccgagtg acccctttgt tggcagactt ggccttcatg tcccctccgc cctgccctgt
     2521 gtcccatggg agcaatggca gagattccca ctgcatcgca ctgcccggtt accctcagca
     2581 gcaaaccctg ggttctaacc ccggcactgg agagcccaac cgtccccagt ggaaccaact
     2641 cagacatcca tgtttcctta ggagcttggg cacaaaggca acggactcct gtgttgttct
     2701 aacagctgtg gagactgagg gacgtgtatg agcgtaaagc acctgcttta cttgccagtc
     2761 tcctgtgtaa attacagtaa aacaaaggaa ctgaaacgga aacaaaataa atttgatctc
     2821 aaaattcaaa aaaaaaaaaa aaaaaaa
//
