LOCUS       NM_178656               3014 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus phosphoinositide-interacting regulator of transient
            receptor potential channels (Pirt), mRNA.
ACCESSION   NM_178656
VERSION     NM_178656.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Espino,C.M., Nagaraja,C., Ortiz,S., Dayton,J.R., Murali,A.R.,
            Ma,Y., Mann,E.L., Garlapalli,S., Wohlgemuth,R.P., Brashear,S.E.,
            Smith,L.R., Wilkinson,K.A. and Griffith,T.N.
  TITLE     Differential encoding of mammalian proprioception by voltage-gated
            sodium channels
  JOURNAL   Sci Adv 11 (2), eads6660 (2025)
   PUBMED   39772670
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Jiang,H., Cui,H., Chen,M., Li,F., Shen,X., Guo,C.J., Hoekel,G.E.,
            Zhu,Y., Han,L., Wu,K., Holtzman,M.J. and Liu,Q.
  TITLE     Divergent sensory pathways of sneezing and coughing
  JOURNAL   Cell 187 (21), 5981-5997 (2024)
   PUBMED   39243765
REFERENCE   3  (bases 1 to 3014)
  AUTHORS   Zhang,L., Nagel,M., Olson,W.P., Chesler,A.T. and O'Connor,D.H.
  TITLE     Trigeminal innervation and tactile responses in mouse tongue
  JOURNAL   Cell Rep 43 (9), 114665 (2024)
   PUBMED   39215998
REFERENCE   4  (bases 1 to 3014)
  AUTHORS   Son,H., Zhang,Y., Shannonhouse,J., Gomez,R. and Kim,Y.S.
  TITLE     PACAP38/mast-cell-specific receptor axis mediates repetitive
            stress-induced headache in mice
  JOURNAL   J Headache Pain 25 (1), 87 (2024)
   PUBMED   38802819
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3014)
  AUTHORS   Jeon,S.M., Pradeep,A., Chang,D., McDonough,L., Chen,Y.,
            Latremoliere,A., Crawford,L.K. and Caterina,M.J.
  TITLE     Skin Reinnervation by Collateral Sprouting Following Spared Nerve
            Injury in Mice
  JOURNAL   J Neurosci 44 (15), e1494232024 (2024)
   PUBMED   38471780
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3014)
  AUTHORS   Kim,Y.S., Chu,Y., Han,L., Li,M., Li,Z., LaVinka,P.C., Sun,S.,
            Tang,Z., Park,K., Caterina,M.J., Ren,K., Dubner,R., Wei,F. and
            Dong,X.
  TITLE     Central terminal sensitization of TRPV1 by descending serotonergic
            facilitation modulates chronic pain
  JOURNAL   Neuron 81 (4), 873-887 (2014)
   PUBMED   24462040
REFERENCE   7  (bases 1 to 3014)
  AUTHORS   Tang,Z., Kim,A., Masuch,T., Park,K., Weng,H., Wetzel,C. and Dong,X.
  TITLE     Pirt functions as an endogenous regulator of TRPM8
  JOURNAL   Nat Commun 4, 2179 (2013)
   PUBMED   23863968
  REMARK    GeneRIF: Pirt regulates the function of TRPM8 and its role in
            detecting cold.
REFERENCE   8  (bases 1 to 3014)
  AUTHORS   Dykes,I.M., Tempest,L., Lee,S.I. and Turner,E.E.
  TITLE     Brn3a and Islet1 act epistatically to regulate the gene expression
            program of sensory differentiation
  JOURNAL   J Neurosci 31 (27), 9789-9799 (2011)
   PUBMED   21734270
REFERENCE   9  (bases 1 to 3014)
  AUTHORS   Patel,K.N., Liu,Q., Meeker,S., Undem,B.J. and Dong,X.
  TITLE     Pirt, a TRPV1 modulator, is required for histamine-dependent and
            -independent itch
  JOURNAL   PLoS One 6 (5), e20559 (2011)
   PUBMED   21655234
  REMARK    GeneRIF: Pirt contributes to both histaminergic and
            nonhistaminergic itch and, crucially, is involved in forms of itch
            that are both TRPV1-dependent and -independent.
REFERENCE   10 (bases 1 to 3014)
  AUTHORS   Kim,A.Y., Tang,Z., Liu,Q., Patel,K.N., Maag,D., Geng,Y. and Dong,X.
  TITLE     Pirt, a phosphoinositide-binding protein, functions as a regulatory
            subunit of TRPV1
  JOURNAL   Cell 133 (3), 475-485 (2008)
   PUBMED   18455988
  REMARK    GeneRIF: Pirt is a key component of the TRPV1 complex and
            positively regulates TRPV1 activity.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AK041182.1.
            
            On Apr 6, 2007 this sequence version replaced NM_178656.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK041182.1, EU447173.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3014              AK041182.1         2-3015
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 40.59 cM"
     gene            1..3014
                     /gene="Pirt"
                     /gene_synonym="A530088H08Rik"
                     /note="phosphoinositide-interacting regulator of transient
                     receptor potential channels"
                     /db_xref="GeneID:193003"
                     /db_xref="MGI:MGI:2443635"
     exon            1..152
                     /gene="Pirt"
                     /gene_synonym="A530088H08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            153..3014
                     /gene="Pirt"
                     /gene_synonym="A530088H08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             179..586
                     /gene="Pirt"
                     /gene_synonym="A530088H08Rik"
                     /codon_start=1
                     /product="phosphoinositide-interacting protein"
                     /protein_id="NP_848771.1"
                     /db_xref="CCDS:CCDS24849.1"
                     /db_xref="GeneID:193003"
                     /db_xref="MGI:MGI:2443635"
                     /translation="MEVLPKALEVDERSPESKDLLPSQTASSLCISSRSESVWTTTPK
                     SNWEIYHKPIIIMSVGAAILLFGVAITCVAYILEEKHKVVQVLRMIGPAFLSLGLMML
                     VCGLVWVPIIKKKQKQRQKSNFFQSLKFFLLNR"
     regulatory      1138..1143
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pirt"
                     /gene_synonym="A530088H08Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      1157
                     /gene="Pirt"
                     /gene_synonym="A530088H08Rik"
                     /note="major polyA site"
     regulatory      1197..1202
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pirt"
                     /gene_synonym="A530088H08Rik"
                     /note="hexamer: AATATA"
     polyA_site      1223
                     /gene="Pirt"
                     /gene_synonym="A530088H08Rik"
                     /note="major polyA site"
ORIGIN      
        1 agtggctggg attcgcagca cactgatgtg gccagggtgc ctttctccag ctgggatgaa
       61 ggacttgagc agaaactcat tgccagcttc ctccctacgc gagagccgac tgagtcccag
      121 gtccccagtc ttcccccggg acgttgtgca cggtgcccat tcttgagcag ccacaacaat
      181 ggaggtgctc cccaaggccc tggaggtaga cgagaggtct ccagagtcca aggacctgct
      241 gcccagccag acagccagct ccctgtgcat cagttccaga agtgagtctg tctggaccac
      301 cacacccaaa agcaactggg aaatctacca caagcccatc atcatcatgt cagtgggagc
      361 tgccattctg ctctttggcg tggccatcac ctgtgtggcc tacatcttgg aagagaagca
      421 taaagttgtg caagtgctca ggatgatagg gcctgccttc ctgtccctgg gactcatgat
      481 gctggtgtgt gggctggtgt gggtccccat aatcaaaaag aagcagaagc aaaggcagaa
      541 gtccaacttc ttccaaagcc tcaagttctt cctcctgaac cgctgatgac tggttgtcca
      601 gaagatctgc taaccaataa gcagcctcct accttctctt cgggtaccac aaagttgatc
      661 caggcaaacc ctcctcttgg ccctgtggac aggatagagc tcagggcttc accctcatac
      721 aacctagcag cattgctgac tgagtctcac ctggtttcca tagctgtgga tgctgtgccc
      781 ttggatactt tcattaccct catccctggc acctgcattc agccatcagc catcccattc
      841 tctctgccaa gggcaatgtg tgcatgctag gaaattcttt gggggttgac tacattccca
      901 aggagaactt gtatgttacg gttgtgtgcc tgatcttaga ttcccatcta catccttctg
      961 gaaccaaaag tgaccaagca gataaggctg acttcagtcc cattgggttt gacagccttg
     1021 gctccctcct tggatgggac attgactaac attacaagag aaaggatatg tctcatgtat
     1081 cacacattcc aaaatctgga cagtgatggg gctgggggtg agggaaacac tgtctagagt
     1141 aaaccattcc tctgggagta gtctggaact tatacagtga aggaagttag ctcctaaata
     1201 tatgatattg gcacaagagg caatatgcag gctaagaggt atcaacactt ccccttgatc
     1261 ctccaatgcg cttcttgcag aatgccttta tattagcaat tagccaagaa caaatgctct
     1321 ttgttctaac ttccttcccc accacatctc tgcgtctaca cagctccaga acagaaggac
     1381 gggaggccac agatgtgacc tgtaagatca tctccttctc ctgtcaatca agacctaacc
     1441 tgaaattgaa tgccatgtcc gactcacgct gcatggggtt ttagagatag gttcactgga
     1501 aaaaaggaaa tctcagcctc cctcctccct gttcctccct accaaacaag caagtattta
     1561 ttgagtttcc ttctctaggc ctaggttggg aacagccaga cccagtctct gatgtcatct
     1621 tatttccaaa agtgaaagag ggaaaaacat ggccaagcca actggcaata ctccatactg
     1681 agttcttagg gtggccatgg gaacacatgg atctaacaaa tgtacaggaa gatagatttc
     1741 tggagaccat gttcacccct tctgaatatg aaggggaagg aagtgtttgg aatgagcaag
     1801 atgtgcaagg tagtcagcaa ctgccttgca tgtggagaag ctaaggggaa agagacaggg
     1861 tggggttagg attccgcata gctcccggat gctattccat cctctcttgc ctacttcccc
     1921 cctgcttccc caggtacctt acatccagct actccttggt acactgcagg cttctggggt
     1981 caatagggac tgggaggggc atctccagag ggcctaacaa gtagatataa cccaagaggt
     2041 aagtaccctc aaaacttcat tatagtcacc aagacacctt taggcaaaag accgggcacc
     2101 tataagaaat ttccaaagct gttccaggca aggccaggcc agagagcaga ggaaggtacc
     2161 tagtagcaaa gtgaatgaca agagctgcat tggttcaggt tgactcttca tccttaacct
     2221 ttgggcattt gggaacacta tggcaaacaa cctccaacag gtctccagat atctcaacca
     2281 ttcacagtac ttctataggc agttagaatc caccaccttt gttcctgttg cattgtggga
     2341 cattcctcgg aggaagtatt tgttttgtgg aatcaacaca cacacacaca cacacacaca
     2401 gagagagaga gagagagaga gagagagaga gagagagaga gaaagaaaga gaaagaaaga
     2461 aagaaagaga aagagactga ctccctaact aaaaagtcag agtttgggaa gcctgtggcc
     2521 tttcaaagct cacttaagaa tatcatgttc ctcattaaga ctcacatcat cgagcccagg
     2581 ccctgcagtc cacccattcc ctgaatacag gcagctcagg accaaccctg gggttgttga
     2641 aatactgcct agtgcttcca cgaatgtcta atgcctccat gacagggctt tcagaccact
     2701 cctttctcct gacatggaag gacagccctg gggtggagcc tctcaatctt ctgtgccttc
     2761 atgaaaggga acacacagat gagctcacag ccagctcact tggaatccgc accccatgca
     2821 cctcattgtc ctgagagctc attgtctggg cacagctgtg ggaagacctt tgcagatctc
     2881 actttcaagt atgtctcaac agaagggagt ttggggataa tcacgatgcc aggaaatctt
     2941 caagttctag acatctttca tagccacatc agtacctgtt ccccaacccc tgcccctcaa
     3001 ggtaagtact tagc
//
