LOCUS       NM_027153               1601 bp    mRNA    linear   ROD 08-OCT-2024
DEFINITION  Mus musculus pirin (Pir), transcript variant 1, mRNA.
ACCESSION   NM_027153 XM_136134
VERSION     NM_027153.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Shen,X., Zhang,J., Zhou,Z. and Yu,R.
  TITLE     PLIN5 Suppresses Lipotoxicity and Ferroptosis in Cardiomyocyte via
            Modulating PIR/NF-kappaB Axis
  JOURNAL   Int Heart J 65 (3), 537-547 (2024)
   PUBMED   38749744
  REMARK    GeneRIF: PLIN5 Suppresses Lipotoxicity and Ferroptosis in
            Cardiomyocyte via Modulating PIR/NF-kappaB Axis.
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Ma,H., Suleman,M., Zhang,F., Cao,T., Wen,S., Sun,D., Chen,L.,
            Jiang,B., Wang,Y., Lin,F., Wang,J., Li,B. and Li,Q.
  TITLE     Pirin Inhibits FAS-Mediated Apoptosis to Support Colorectal Cancer
            Survival
  JOURNAL   Adv Sci (Weinh) 11 (10), e2301476 (2024)
   PUBMED   38148593
  REMARK    GeneRIF: Pirin Inhibits FAS-Mediated Apoptosis to Support
            Colorectal Cancer Survival.
REFERENCE   3  (bases 1 to 1601)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1601)
  AUTHORS   Brzoska,K., Stepkowski,T.M. and Kruszewski,M.
  TITLE     Putative proto-oncogene Pir expression is significantly
            up-regulated in the spleen and kidney of cytosolic superoxide
            dismutase-deficient mice
  JOURNAL   Redox Rep 16 (3), 129-133 (2011)
   PUBMED   21801495
  REMARK    GeneRIF: Sod1(-/-) mice have a significantly elevated level of Pir
            mRNA in the spleen and kidney but not in the liver, heart, or/and
            brain.
REFERENCE   5  (bases 1 to 1601)
  AUTHORS   Cox,B.J., Vollmer,M., Tamplin,O., Lu,M., Biechele,S.,
            Gertsenstein,M., van Campenhout,C., Floss,T., Kuhn,R., Wurst,W.,
            Lickert,H. and Rossant,J.
  TITLE     Phenotypic annotation of the mouse X chromosome
  JOURNAL   Genome Res 20 (8), 1154-1164 (2010)
   PUBMED   20548051
REFERENCE   6  (bases 1 to 1601)
  AUTHORS   Licciulli,S., Cambiaghi,V., Scafetta,G., Gruszka,A.M. and
            Alcalay,M.
  TITLE     Pirin downregulation is a feature of AML and leads to impairment of
            terminal myeloid differentiation
  JOURNAL   Leukemia 24 (2), 429-437 (2010)
   PUBMED   20010624
REFERENCE   7  (bases 1 to 1601)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   8  (bases 1 to 1601)
  AUTHORS   Calvo,S.E., Pagliarini,D.J. and Mootha,V.K.
  TITLE     Upstream open reading frames cause widespread reduction of protein
            expression and are polymorphic among humans
  JOURNAL   Proc Natl Acad Sci U S A 106 (18), 7507-7512 (2009)
   PUBMED   19372376
  REMARK    GeneRIF: Includes the study of an upstream ORF in this gene, and
            shows that it functions to reduce protein levels by ~38%.
REFERENCE   9  (bases 1 to 1601)
  AUTHORS   Wendler,W.M., Kremmer,E., Forster,R. and Winnacker,E.L.
  TITLE     Identification of pirin, a novel highly conserved nuclear protein
  JOURNAL   J Biol Chem 272 (13), 8482-8489 (1997)
   PUBMED   9079676
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY718294.1, AK009757.1 and AL732475.6.
            
            On May 5, 2010 this sequence version replaced NM_027153.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC024062.2, AK138314.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            regulatory uORF        :: PMID: 19372376
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-81                BY718294.1         2-82
            82-1370             AK009757.1         25-1313
            1371-1601           AL732475.6         63057-63287
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 76.12 cM"
     gene            1..1601
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /note="pirin"
                     /db_xref="GeneID:69656"
                     /db_xref="MGI:MGI:1916906"
     exon            1..247
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      15..53
                     /regulatory_class="uORF"
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:19372376]"
                     /note="this uORF is predicted to encode a 12 aa peptide
                     (PMID:19372376)"
     exon            248..376
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /inference="alignment:Splign:2.1.0"
     CDS             281..1153
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /EC_number="1.13.11.24"
                     /note="isoform 1 is encoded by transcript variant 1;
                     probable quercetinase; probable quercetin 2,3-dioxygenase
                     PIR; iron-binding nuclear protein; pirin like"
                     /codon_start=1
                     /product="pirin isoform 1"
                     /protein_id="NP_081429.1"
                     /db_xref="CCDS:CCDS30520.1"
                     /db_xref="GeneID:69656"
                     /db_xref="MGI:MGI:1916906"
                     /translation="MASSKKVTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDE
                     FKGGKPGGFPDHPHRGFETVSYLLEGGSMAHEDFCGHVGKMNPGDLQWMTAGRGILHA
                     EMPCSEEPAHGLQLWVNLRRSEKMVAPQYQELKSEEIPKPTKDGVTVAVISGEALGIK
                     SKVYTRTPTLYLDFKLDQGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQKIEPHHTA
                     VLGEGDAVQLENKDPKRSHFVLIAGEPLREPVVQHGPFVMNTNEEISQAILDFRNAKN
                     GFEGARTWKSKIGN"
     exon            377..469
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /inference="alignment:Splign:2.1.0"
     exon            470..553
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /inference="alignment:Splign:2.1.0"
     exon            554..760
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /inference="alignment:Splign:2.1.0"
     exon            761..845
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /inference="alignment:Splign:2.1.0"
     exon            846..890
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /inference="alignment:Splign:2.1.0"
     exon            891..973
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1040
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1601
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1355..1360
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /note="hexamer: AATAGA"
     polyA_site      1375
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /note="major polyA site"
     regulatory      1468..1473
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /note="hexamer: AATAAA"
     polyA_site      1497
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
     regulatory      1580..1585
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
                     /note="hexamer: TATAAA"
     polyA_site      1601
                     /gene="Pir"
                     /gene_synonym="2310042L19Rik; Pirnl"
ORIGIN      
        1 agatccagag agccatgggg cggagtcaga gaatccaaac ccagggtaaa taaagggtgt
       61 gtcccccggt ccaaggcccc tgagacctag agactcccgc cttctggagg cccgcaacaa
      121 agcgctgagt cacgctgact gacggctggc gctccgcaaa cctgtcctcc ctttgctgtg
      181 tccctgccgc tgtggagtct aggcacctcc gactgtggcc tccctcctgg gcctgcgcta
      241 ctgcccactc aacatcagaa ctttctttat aaacttcaaa atggcatcct ctaaaaaggt
      301 tactctctca gtgctcagcc gggagcagtc agagggggta ggagcacgtg tgcgcagaag
      361 cattggcaga cccgagttga aaaatctgga tccattttta ctgtttgatg aatttaaagg
      421 aggaaagccg ggaggatttc ctgatcatcc acaccgagga tttgaaacag tatcttatct
      481 tctggaaggg ggcagcatgg cccatgaaga cttttgtgga catgttggta aaatgaaccc
      541 aggagaccta cagtggatga ctgcaggacg aggcattctg catgctgaga tgccctgctc
      601 agaggagcca gctcatggct tgcagctgtg ggtgaatttg agacgctcag aaaagatggt
      661 ggcgcctcag taccaggaac tgaaaagtga agagatcccg aaacccacca aagatggtgt
      721 gacagttgct gtcatttcag gagaagcctt gggaataaag tcgaaggttt acactcgcac
      781 accaacctta tatctggact tcaagttgga ccaaggagca aagcattccc agcctattcc
      841 taaagggtgg acaagcttca tttataccat ctctggagat gtatatattg ggcctgatga
      901 tgctcaacaa aagatagaac cacatcacac agcagtcctt ggagaaggtg acgctgtcca
      961 gttggaaaat aaggacccca aaagaagcca ttttgtctta attgctgggg agccattaag
     1021 agaaccagtt gtccaacatg gtccatttgt gatgaatacc aatgaggaaa tttctcaggc
     1081 cattcttgat ttcagaaatg caaaaaatgg ttttgagggg gccagaacct ggaagtcaaa
     1141 gattggaaac taatgaagag ctgagacgct gccacttgga tggactggcc attcggtata
     1201 ttaaatttcc aaggctagtt tagctggtgc ttctgaagcc acattaagct gggcttagtg
     1261 atgcagagtc ctttaatccc agcactctag gagtcagaga cattgggtat ctccgatttc
     1321 caagcccatc tgtcttgaaa atggaaaaat aaaaaataga attccacatt aaaaagatgt
     1381 ttttttgtaa tgaatcttca gaatgcatta tattcatgtt tgaaattgtc aaagaatatg
     1441 tttaatttaa aaaggaaaac tacaaacaat aaaaaaatgg cagtaacata tgtaaaatat
     1501 ttcctgatac atgcaaataa ttttacttgt tgcttttaaa aaatcaatgt tcttcatttg
     1561 gtagaaatta ttaagcacct ataaaaatta attgctattt a
//
