LOCUS       NM_008847               3673 bp    mRNA    linear   ROD 01-FEB-2024
DEFINITION  Mus musculus phosphatidylinositol-4-phosphate 5-kinase, type 1
            alpha (Pip5k1a), transcript variant 2, mRNA.
ACCESSION   NM_008847
VERSION     NM_008847.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Sirisin,J., Kamnate,A., Polsan,Y., Somintara,S., Chomphoo,S.,
            Sakagami,H., Kondo,H. and Hipkaeo,W.
  TITLE     Localization of phosphatidylinositol 4-phosphate 5-kinase (PIP5K)
            alpha confined to the surface of lipid droplets and adjacent narrow
            cytoplasm in progesterone-producing cells of in situ ovaries of
            adult mice
  JOURNAL   Acta Histochem 123 (7), 151794 (2021)
   PUBMED   34624591
  REMARK    GeneRIF: Localization of phosphatidylinositol 4-phosphate 5-kinase
            (PIP5K) alpha confined to the surface of lipid droplets and
            adjacent narrow cytoplasm in progesterone-producing cells of in
            situ ovaries of adult mice.
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Chen,X., Wan,J., Yu,B., Diao,Y. and Zhang,W.
  TITLE     PIP5K1alpha promotes myogenic differentiation via AKT activation
            and calcium release
  JOURNAL   Stem Cell Res Ther 9 (1), 33 (2018)
   PUBMED   29426367
  REMARK    GeneRIF: PIP5K1alpha was found to be required for AKT activation
            and calcium release, both of which were important for skeletal
            muscle differentiation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3673)
  AUTHORS   Nguyen,T.T.N., Seo,E., Choi,J., Le,O.T.T., Kim,J.Y., Jou,I. and
            Lee,S.Y.
  TITLE     Phosphatidylinositol 4-phosphate 5-kinase alpha contributes to
            Toll-like receptor 2-mediated immune responses in microglial cells
            stimulated with lipoteichoic acid
  JOURNAL   Cell Signal 38, 159-170 (2017)
   PUBMED   28711717
  REMARK    GeneRIF: these results support that PIP5Kalpha can positively
            mediate TLR2-associated immune responses through
            Phosphatidylinositol 4,5-bisphosphate production in microglial
            cells.
REFERENCE   4  (bases 1 to 3673)
  AUTHORS   Liu,T., Yu,B., Kakino,M., Fujimoto,H., Ando,Y., Hakuno,F. and
            Takahashi,S.I.
  TITLE     A novel IRS-1-associated protein, DGKzeta regulates GLUT4
            translocation in 3T3-L1 adipocytes
  JOURNAL   Sci Rep 6, 35438 (2016)
   PUBMED   27739494
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3673)
  AUTHORS   Wright,B.D., Loo,L., Street,S.E., Ma,A., Taylor-Blake,B.,
            Stashko,M.A., Jin,J., Janzen,W.P., Frye,S.V. and Zylka,M.J.
  TITLE     The lipid kinase PIP5K1C regulates pain signaling and sensitization
  JOURNAL   Neuron 82 (4), 836-847 (2014)
   PUBMED   24853942
REFERENCE   6  (bases 1 to 3673)
  AUTHORS   Tolias,K.F., Hartwig,J.H., Ishihara,H., Shibasaki,Y., Cantley,L.C.
            and Carpenter,C.L.
  TITLE     Type Ialpha phosphatidylinositol-4-phosphate 5-kinase mediates
            Rac-dependent actin assembly
  JOURNAL   Curr Biol 10 (3), 153-156 (2000)
   PUBMED   10679324
REFERENCE   7  (bases 1 to 3673)
  AUTHORS   Anderson,R.A., Boronenkov,I.V., Doughman,S.D., Kunz,J. and
            Loijens,J.C.
  TITLE     Phosphatidylinositol phosphate kinases, a multifaceted family of
            signaling enzymes
  JOURNAL   J Biol Chem 274 (15), 9907-9910 (1999)
   PUBMED   10187762
  REMARK    Review article
REFERENCE   8  (bases 1 to 3673)
  AUTHORS   Ishihara,H., Shibasaki,Y., Kizuki,N., Wada,T., Yazaki,Y., Asano,T.
            and Oka,Y.
  TITLE     Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the
            third isoform and deletion/substitution analysis of members of this
            novel lipid kinase family
  JOURNAL   J Biol Chem 273 (15), 8741-8748 (1998)
   PUBMED   9535851
REFERENCE   9  (bases 1 to 3673)
  AUTHORS   Davis,J.N., Rock,C.O., Cheng,M., Watson,J.B., Ashmun,R.A., Kirk,H.,
            Kay,R.J. and Roussel,M.F.
  TITLE     Complementation of growth factor receptor-dependent mitogenic
            signaling by a truncated type I phosphatidylinositol 4-phosphate
            5-kinase
  JOURNAL   Mol Cell Biol 17 (12), 7398-7406 (1997)
   PUBMED   9372970
REFERENCE   10 (bases 1 to 3673)
  AUTHORS   Ishihara,H., Shibasaki,Y., Kizuki,N., Katagiri,H., Yazaki,Y.,
            Asano,T. and Oka,Y.
  TITLE     Cloning of cDNAs encoding two isoforms of 68-kDa type I
            phosphatidylinositol-4-phosphate 5-kinase
  JOURNAL   J Biol Chem 271 (39), 23611-23614 (1996)
   PUBMED   8798574
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC131769.3, AK158062.1 and AC087062.25.
            
            [WARNING] On Mar 20, 2024 this sequence was replaced by
            NM_008847.4.
            
            On Jun 17, 2014 this sequence version replaced NM_008847.2.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region compared to variant 1. The
            encoded isoform (2) is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK158062.1, BC031774.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                AC131769.3         94131-94185         c
            56-381              AK158062.1         1-326
            382-382             AC131769.3         93804-93804         c
            383-2609            AK158062.1         328-2554
            2610-2612           AC087062.25        221016-221018       c
            2613-3631           AK158062.1         2555-3573
            3632-3673           AC087062.25        219955-219996       c
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 40.74 cM"
     gene            1..3673
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /note="phosphatidylinositol-4-phosphate 5-kinase, type 1
                     alpha"
                     /db_xref="GeneID:18720"
                     /db_xref="MGI:MGI:107929"
     exon            1..555
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     CDS             480..2120
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /EC_number="2.7.1.68"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ptdIns(4)P-5-kinase 1 alpha; phosphatidylinositol
                     4-phosphate 5-kinase type I alpha; 68 kDa type I
                     phosphatidylinositol 4-phosphate 5-kinase; 68 kDa type I
                     phosphatidylinositol 4-phosphate 5-kinase alpha"
                     /codon_start=1
                     /product="phosphatidylinositol 4-phosphate 5-kinase type-1
                     alpha isoform 2"
                     /protein_id="NP_032873.2"
                     /db_xref="CCDS:CCDS38542.1"
                     /db_xref="GeneID:18720"
                     /db_xref="MGI:MGI:107929"
                     /translation="MASASSGPAAAGFSSLDAGAPAGTAAASGIKRATVSEGPSASVM
                     PVKKIGHRSVDSSGETTYKKTTSSALKGAIQLGITHTVGSLSTKPERDVLMQDFYVVE
                     SIFFPSEGSNLTPAHHYNDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCSEPLIELSN
                     SGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQ
                     AGGKNIRIVVMNNLLPRSVKMHMKYDLKGSTYKRRASQKEREKTLPTFKDLDFLQDIP
                     DGLFLDADMYSALCKTLQRDCLVLQSFKIMDYSLLMSIHNMDHAQREPTSNDTQYSAD
                     TRRPAPQKALYSTAMESIQGEARRGGTVETEDHMGGIPARNNKGERLLLYIGIIDILQ
                     SYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPLKPSPTKKFRS
                     GPSFSRRSGPSGNSCTSQLMASGEHRAQVTTKAEVEPDVHLGRPDVLPQTPPLEEISE
                     GSPVPGPSFSPVVGQPLQILNLSSTLEKLDVAESEFTH"
     exon            556..590
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            591..671
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            672..802
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            803..920
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1073
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1373
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1579
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1663
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1712
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1797
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..1941
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            1942..2071
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2117
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
     exon            2118..3673
                     /gene="Pip5k1a"
                     /gene_synonym="PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha;
                     Pipk5a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cttttctccg gctagataga cgaacgccga gaagctgtgg cgcatgcgca ataccggggt
       61 ttccttggcc gcccaccctg agccgagcgg tggagttggg gataaacagg cagtggctgg
      121 ggaggtgagg gagggggggc attcgctttt gggggcacct caggtgccta ggttttcctt
      181 taggtttgtt ctatccgaga cgcgggactc ccggggcccg gctcaagtgt tcctgtctgg
      241 taagagaagg tagctttggc ggagagcagt tcaagttgct aggaaggtgc gagactgcag
      301 ctctccccca tgccgggcaa gtggtgcggt cctgagcttg tcctggagcc tgaacaacgc
      361 ctgtgagaag cccgccgaac gcgggttctg tgaagagacg tggggaagat tcgattccga
      421 gaaaaggaaa agccggattg aaagggagcg aagccgctga gggggagggg gctgccaaga
      481 tggcgtccgc ctcctcaggg ccagcggcgg ccgggttttc atcccttgat gccggggccc
      541 ctgctggtac cgcagcagca tctggaatca agagagccac agtatctgag ggaccttccg
      601 cctctgtcat gcctgttaaa aaaataggcc atcgaagtgt tgattcctct ggagagacta
      661 catacaagaa gacaacttca tcagccttga aaggtgccat ccagttaggc atcactcaca
      721 ctgtgggcag cctgagcacc aaaccagagc gtgatgtcct catgcaagac ttctacgtgg
      781 tggagagcat ctttttcccc agtgaaggca gcaacctgac accggctcac cattacaatg
      841 actttcgatt caagacctat gcgcctgttg ccttccgtta cttcagagag ctctttggca
      901 tccggcctga tgattacttg tactcccttt gcagtgagcc attgattgaa ctctccaatt
      961 ctggagctag tggttccctc ttctatgtgt ccagtgatga tgaattcatc attaagaccg
     1021 tccagcataa agaagcagaa tttctgcaga agttgcttcc aggatactac atgaatctta
     1081 accaaaaccc tcgtactttg ctgcccaaat tttatggatt gtactgtgtg caagcaggcg
     1141 gcaagaacat acgaattgtg gtgatgaaca atctcttgcc tcggtcagtc aaaatgcata
     1201 tgaaatatga cctgaagggt tcaacttaca agcggcgggc ttctcagaaa gaacgagaga
     1261 aaactctccc cacctttaaa gacctggact tcctacaaga tatccctgac ggcctatttt
     1321 tggatgctga catgtacagt gctctgtgta agactctaca gcgtgactgt ttggtgctgc
     1381 agagcttcaa gataatggac tacagcctgt tgatgtccat ccacaacatg gaccatgcac
     1441 agagggagcc cacgagcaat gacacacagt actcggctga cacgcgcaga ccagccccac
     1501 agaaggcgct ctattccaca gctatggaat ccatccaggg cgaggctcga cggggcggca
     1561 ctgtggagac tgaggaccac atgggtggca tccctgcccg gaataacaaa ggagaaaggc
     1621 tcctgcttta tattggcatc attgatattc ttcagtccta caggtttgtt aagaagttgg
     1681 agcactcttg gaaagcactg gtccatgatg gggatacggt gtcagtgcat cgtccaggct
     1741 tctatgctga gcggttccag cgtttcatgt gcaacacagt gttcaagaag attcccttga
     1801 agccttctcc taccaaaaag tttcggtctg gcccgtcttt ctctcggcga tcaggcccca
     1861 gcggcaactc ctgcacctcc cagctgatgg cctctgggga acacagagca caagtgacca
     1921 ccaaggcgga agtggagcca gatgtacacc ttgggcgtcc tgatgtctta cctcagactc
     1981 cacctttgga ggaaataagt gagggttcac ctgttcctgg ccccagtttc tcacctgtag
     2041 ttggacaacc tttgcaaata ctaaatttga gttcaacctt ggaaaagctt gatgttgcag
     2101 agtcagagtt cacccactga gcagtgagcc tcagaagacc tgaaacaaga ctctgctgtc
     2161 gctttgatcc caagatgtca gcccttgccc caggaatgct gaatcgtctt cttggtcatc
     2221 aacagaggag agaatgtgga gctagcaggg ctctgacact gtctcccctt cctctgggca
     2281 gtagtgcctt tgacagttga ggacagcatc gtccccacca ctccagaagt gggtggcgtg
     2341 aattttccac tgcctgccat ggcttctata gctgaatatt tttcaaaccc ccattctttg
     2401 tgctgacatg gggttgctgg actggtggtt tcctcctgtt tacctttctc ctggagctgg
     2461 actcttcatt tcctcaggac ggactggctg gacattgttc ctgccttggc tctcccgctg
     2521 gtctttggag actcccaatg atttgtataa aggtgtttgg gggaaagtaa ggatcatcct
     2581 tactcccaac cttcttttcc ttaaaaaagg aaaaaaaaaa aaaaaaaaaa cacaaagagc
     2641 acacaatgta aatttgagcc agtctcagat aaaagttctc taagtgttgt agaagagccc
     2701 tagtgtttgt gaagcagaaa ggctggggct gccccaccag cagctgctct ctgactccct
     2761 cacttagggt tgagggaata gagagggcct ggcccttttt taagacaaag gggcagccat
     2821 gttaggagag tggaccttag caggacccta ctcactgcca ggtcttcggt tattactatt
     2881 ctgcaatttg gaatgtatcc tcatagtttt ttctagatat aaagaattat caaatgattt
     2941 tagaccattc tccaaaggag atttctttgt tcttcctata ttttccaaca gtattctgct
     3001 ctttgtggag ctacggtgga gggagatgtt tgtgtctgtt taacaggcag tctgtatcta
     3061 tgaggctaga gaatattttc ttaaactcct agggagccac agacttccct ctgtacggtt
     3121 gtcgccatgt cacctggcct gcccttactc tgcagagaag ctggaaatgg ggccacggag
     3181 ccctctcctt cctctcagtt gtggcccatc tggtcctggc tggtggggca gtgcatggtg
     3241 tttcaggctg gctgttgaaa tcccacatta gttccttccc tgtcccgtcc caccttgata
     3301 gcaggttagc caacccccac gtaactgtct atattagaca cccccagcca ccttctggct
     3361 gcctgtcttt gctgccatgt tcttttttac aagaaggaaa caaacaattt cttgctattt
     3421 ttttttcata atttactatt tatgatgtat ttaagtgttt tattcaggac agggttctgt
     3481 aaggggtggg agggagtatt tgagggaggg ctgggactta gggaaaggac atgggattca
     3541 acatttttat ggagtgttac tatttgcctc tactttgtat tgttgaaaaa tgacagatgc
     3601 aatataaaag taataaatat ctggttttaa tgtattaaag ttctagcaat catttagacc
     3661 tatttttaaa tgc
//
