LOCUS       NM_008845               3471 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus phosphatidylinositol-5-phosphate 4-kinase, type II,
            alpha (Pip4k2a), transcript variant 1, mRNA.
ACCESSION   NM_008845 XM_985285 XM_990996 XM_994696
VERSION     NM_008845.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Triscott,J., Reist,M., Kung,L., Mosele,F.C., Lehner,M., Gallon,J.,
            Ravi,A., Arora,G.K., de Brot,S., Lundquist,M., Gallart-Ayala,H.,
            Ivanisevic,J., Piscuoglio,S., Cantley,L.C., Emerling,B.M. and
            Rubin,M.A.
  TITLE     PI5P4Kalpha supports prostate cancer metabolism and exposes a
            survival vulnerability during androgen receptor inhibition
  JOURNAL   Sci Adv 9 (5), eade8641 (2023)
   PUBMED   36724278
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Ravi,A., Palamiuc,L., Loughran,R.M., Triscott,J., Arora,G.K.,
            Kumar,A., Tieu,V., Pauli,C., Reist,M., Lew,R.J., Houlihan,S.L.,
            Fellmann,C., Metallo,C., Rubin,M.A. and Emerling,B.M.
  TITLE     PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria
            interplay
  JOURNAL   Dev Cell 56 (11), 1661-1676 (2021)
   PUBMED   33984270
REFERENCE   3  (bases 1 to 3471)
  AUTHORS   Noch,E.K., Yim,I., Milner,T.A. and Cantley,L.C.
  TITLE     Distribution and localization of phosphatidylinositol 5-phosphate,
            4-kinase alpha and beta in the brain
  JOURNAL   J Comp Neurol 529 (2), 434-449 (2021)
   PUBMED   32449185
REFERENCE   4  (bases 1 to 3471)
  AUTHORS   Lundquist,M.R., Goncalves,M.D., Loughran,R.M., Possik,E.,
            Vijayaraghavan,T., Yang,A., Pauli,C., Ravi,A., Verma,A., Yang,Z.,
            Johnson,J.L., Wong,J.C.Y., Ma,Y., Hwang,K.S., Weinkove,D.,
            Divecha,N., Asara,J.M., Elemento,O., Rubin,M.A., Kimmelman,A.C.,
            Pause,A., Cantley,L.C. and Emerling,B.M.
  TITLE     Phosphatidylinositol-5-Phosphate 4-Kinases Regulate Cellular Lipid
            Metabolism By Facilitating Autophagy
  JOURNAL   Mol Cell 70 (3), 531-544 (2018)
   PUBMED   29727621
REFERENCE   5  (bases 1 to 3471)
  AUTHORS   Ketel,K., Krauss,M., Nicot,A.S., Puchkov,D., Wieffer,M., Muller,R.,
            Subramanian,D., Schultz,C., Laporte,J. and Haucke,V.
  TITLE     A phosphoinositide conversion mechanism for exit from endosomes
  JOURNAL   Nature 529 (7586), 408-412 (2016)
   PUBMED   26760201
REFERENCE   6  (bases 1 to 3471)
  AUTHORS   Vlangos,C.N., Siuniak,A.N., Robinson,D., Chinnaiyan,A.M.,
            Lyons,R.H. Jr., Cavalcoli,J.D. and Keegan,C.E.
  TITLE     Next-generation sequencing identifies the Danforth's short tail
            mouse mutation as a retrotransposon insertion affecting Ptf1a
            expression
  JOURNAL   PLoS Genet 9 (2), e1003205 (2013)
   PUBMED   23437000
REFERENCE   7  (bases 1 to 3471)
  AUTHORS   Volpicelli-Daley,L.A., Lucast,L., Gong,L.W., Liu,L., Sasaki,J.,
            Sasaki,T., Abrams,C.S., Kanaho,Y. and De Camilli,P.
  TITLE     Phosphatidylinositol-4-phosphate 5-kinases and phosphatidylinositol
            4,5-bisphosphate synthesis in the brain
  JOURNAL   J Biol Chem 285 (37), 28708-28714 (2010)
   PUBMED   20622009
REFERENCE   8  (bases 1 to 3471)
  AUTHORS   Schulze,H., Korpal,M., Hurov,J., Kim,S.W., Zhang,J., Cantley,L.C.,
            Graf,T. and Shivdasani,R.A.
  TITLE     Characterization of the megakaryocyte demarcation membrane system
            and its role in thrombopoiesis
  JOURNAL   Blood 107 (10), 3868-3875 (2006)
   PUBMED   16434494
REFERENCE   9  (bases 1 to 3471)
  AUTHORS   Akiba,Y., Suzuki,R., Saito-Saino,S., Owada,Y., Sakagami,H.,
            Watanabe,M. and Kondo,H.
  TITLE     Localization of mRNAs for phosphatidylinositol phosphate kinases in
            the mouse brain during development
  JOURNAL   Brain Res Gene Expr Patterns 1 (2), 123-133 (2002)
   PUBMED   15018809
REFERENCE   10 (bases 1 to 3471)
  AUTHORS   Ishihara,H., Shibasaki,Y., Kizuki,N., Wada,T., Yazaki,Y., Asano,T.
            and Oka,Y.
  TITLE     Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the
            third isoform and deletion/substitution analysis of members of this
            novel lipid kinase family
  JOURNAL   J Biol Chem 273 (15), 8741-8748 (1998)
   PUBMED   9535851
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844855.15 and AK040558.1.
            
            On or before Feb 21, 2007 this sequence version replaced
            XM_985285.1, XM_990996.1, XM_994696.1, NM_008845.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK040558.1, AK012196.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-110               AL844855.15        59363-59472         c
            111-1597            AK040558.1         1-1487
            1598-3471           AK040558.1         1489-3362
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2"
     gene            1..3471
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /note="phosphatidylinositol-5-phosphate 4-kinase, type II,
                     alpha"
                     /db_xref="GeneID:18718"
                     /db_xref="MGI:MGI:1298206"
     exon            1..270
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..1344
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /EC_number="2.7.1.149"
                     /note="isoform 1 is encoded by transcript variant 1;
                     PIP4KII-alpha; PI(5)P 4-kinase type II alpha;
                     diphosphoinositide kinase 2-alpha;
                     phosphatidylinositol-4-phosphate 5-kinase, type II, alpha;
                     1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha;
                     PI5P4Kalpha; phosphatidylinositol 5-phosphate 4-kinase
                     type-2 alpha"
                     /codon_start=1
                     /product="phosphatidylinositol 5-phosphate 4-kinase type-2
                     alpha isoform 1"
                     /protein_id="NP_032871.3"
                     /db_xref="CCDS:CCDS15712.1"
                     /db_xref="GeneID:18718"
                     /db_xref="MGI:MGI:1298206"
                     /translation="MATPGNLGSSVLASKTKTKKKHFVAQKVKLFRASDPLLSVLMWG
                     VNHSINELSHVQIPVMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLR
                     ERFGIDDQDFQNSLTRSAPLPNDSQARSGARFHTSYDKRYVIKTITSEDVAEMHNILK
                     KYHQYIVECHGVTLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVA
                     REASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKIFLEKLKKDVEFLAQLKLMDYSL
                     LVGIHDVERAEQEEVECEENDGEEEGESDSTHPIGTPPDSPGNTLNSSPPLAPGEFDP
                     NIDVYAIKCHENAPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQ
                     YSKRFLDFIGHIL"
     exon            271..368
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            369..465
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            466..618
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            619..765
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            766..804
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            805..918
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1162
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1266
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..3471
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3447..3452
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /note="putative"
     polyA_site      3471
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /note="putative"
ORIGIN      
        1 ggggctggag caggcgcgca gcggcggccg ccccggggga tgcagctgcc tccccgggct
       61 ggcgtgtagg gagggcgggt ccccggcctc ggcggcgcgg cggtggaggg gaccgagact
      121 gcgaccatgg cgacccctgg caacctgggg tcctccgtcc tggcgagcaa gaccaagacg
      181 aagaagaagc acttcgtggc tcagaaagtg aagctgttcc gggccagcga cccgctgctc
      241 agcgtgctca tgtggggggt caaccattcg atcaatgaac tgagccacgt tcaaatccct
      301 gtcatgttga tgcctgatga cttcaaagcc tactcaaaga taaaggttga caaccacctt
      361 tttaacaagg aaaacatgcc gagccatttc aagtttaagg aatactgccc aatggtcttc
      421 cggaatctgc gggagaggtt tggaatcgac gaccaagatt tccagaattc cttgaccaga
      481 agtgcacccc ttcccaatga ctcccaggct cgcagcgggg ctcggtttca cacgtcttat
      541 gataaaagat acgtcatcaa gaccattacc agtgaggacg tggcagagat gcacaacatc
      601 ctgaagaagt accaccagta tatagtggaa tgtcatgggg tcacacttct tcctcagttc
      661 ttgggaatgt accggcttaa tgtcgatgga gtggaaatat atgtgattgt tacaaggaat
      721 gtgttcagcc accggctatc tgtatatagg aaatacgact taaagggctc gacagtggct
      781 agagaagcta gtgataaaga aaaggccaaa gagctgccaa ctttaaagga taatgacttc
      841 atcaatgagg gccaaaagat ctatattgat gacaacaaca aaaagatatt cctagaaaaa
      901 ctgaaaaagg atgtcgagtt ccttgcacag ttaaaactca tggactacag cctcctggtg
      961 gggattcacg atgtggagcg agcagagcag gaggaggtgg agtgtgagga gaacgatggg
     1021 gaagaagaag gagagagtga cagcacccac ccgatcggga cgccaccaga cagtcccgga
     1081 aatacactca acagctcacc acccttggct cctggagagt ttgatccaaa cattgatgtt
     1141 tatgcaatta aatgccatga gaatgcgcct agaaaagagg tatacttcat ggcgattatt
     1201 gacatcctta ctcattacga tgcaaaaaag aaagctgccc acgctgcaaa aactgttaaa
     1261 catggcgctg gagctgagat ctcgactgtg aacccagagc agtattccaa gcgctttttg
     1321 gactttattg ggcacatctt gtaaccttct gcacagcccc ggatgggcat gaactcggga
     1381 aggctggtgg tggcttcagg gtaggaagaa aaggaaaacc gaaccccagg aagcgctcac
     1441 cttgcaggaa gcaagccttg tttacatctt caagctgaca tgactgaagt tgggggctac
     1501 tcgctttaac agctacctga tatttcccag catcgctatt tctgactctg tacgtgtgca
     1561 cacacgtgcg tgcacacact ctcgcgcaca tttttttaaa taattaatta attaattaat
     1621 taaaactgat cagcttgaat cagagcttgt aacagccaga cccctctgat tccagctgtg
     1681 gccccacgga tagatgaaga gatgagcccg tgggggaagg gaacggaaat gcacctcaat
     1741 gcagcaccac aagagaaact gtagatcagt ttgttttctg acttgaaaga tatgagattg
     1801 ttcatcaggg tggtggtggt ggtggtgatg atgatgatca tgctcataat gatggtgatt
     1861 aaaagaaaga aaattttata tcaaatgtta cacttctggc ccacagaaag actactctga
     1921 atagtggtta cctaagccca cagccctggt aaagggatac aagcatctcc ctgtgtactg
     1981 gttcagggtt ccctcctaaa ctgtcaacat ccccagatgt cccaggctaa gcccttcatc
     2041 ttccaagagt ctgatgccaa gaacctgtca acaatttcac ctccaggaaa accacttgca
     2101 tttctcttct cgcctggagc ccatcactgt taccatcctt aagttgcact gcagaaagtc
     2161 actctgcaaa acagatgtaa aacgtccctg aaaggtgcat agtttggggc aaatgcggct
     2221 acatctccca atgcaggaag atgagcaggg agttcaaacc ctccagtggc cgaaggcaaa
     2281 cccagttatg gtttatgttt agccattgtt taggaatgga attttgttat gaattatccc
     2341 tttaactgaa accctcagtt ttactgttta catggttaga gaaacaggga tatttttgaa
     2401 tctaaaaatt taatatacag catgtgattt ttgaagttta catgtaaagc cattttgcag
     2461 tgtagggaac ataatgtttg ttgtgttttg agatatgtct tgcagttgtg tttctgagtg
     2521 agtgttttgt tgagagtact tagtagacag tctatcacat aaaagcaaaa ggattttgtt
     2581 cctcaagatg tgtatttatc aatctagtgg ggtttttttt ctataaaaaa attaaactca
     2641 aaatctggtt atgtaagttc attgccctaa actgtgctga tcgtgtgtaa ctgagattaa
     2701 aaaacaaaac aaaacaaaaa ccttccagca tcaattatgt atcttccaac ttccctgcat
     2761 tcctaaaagg cctcaagtta cagagtccta gatttgctta gagtccatta ccaacttaca
     2821 cgctgtttgg cagctgtgcc tttaaaagcc cctgtctcaa atgtgagatg gtttctgtag
     2881 gcagttttag aaaggaaaaa atccaccctt caataattac aagtcaacaa gtcccctgcc
     2941 aggctgcctt cccagtcact ttccttttgg cctctccagg tccagctgtg cccaacatgt
     3001 gtgggctgcc ttctcagcat ctggaagcca gcatcagcac catctgactc ctgtgtactg
     3061 acctgccaca gatgcattag cggatgatgc taactcactg taacatagtt gtacacatct
     3121 gtacctgatg catttaaaat aaccagttga ctccgtttcc gggcactgtt aactgtggtg
     3181 tccgatccgt ttgagtgttc tgaagctctg ctcttaacct ttgatcccca aacttacaaa
     3241 cccgagaccc taaagttaaa aaaatgaagg tacatataca tgatctgagt aaccatttcc
     3301 tctgtggcca atctgtatgc ttttagaagt ttacaaaact gctttggttt ttgtctgtaa
     3361 ccatctctgt catcccaata agctgtttgg acaagagcga gaacagtgct aacagtgtgc
     3421 gctcgagtca tgatccgggc tttacgaata aagcactttg atgactctct t
//
