LOCUS       NM_001310624           11390 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus phosphoinositide kinase, FYVE type zinc finger
            containing (Pikfyve), transcript variant 1, mRNA.
ACCESSION   NM_001310624 XM_006495780
VERSION     NM_001310624.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Prado,D.S., Cattley,R.T., Sonego,A.B., Sutariya,P., Wu,S., Lee,M.,
            Boggess,W.C., Shlomchik,M.J. and Hawse,W.F.
  TITLE     The phospholipid kinase PIKFYVE is essential for Th17
            differentiation
  JOURNAL   J Exp Med 222 (2) (2025)
   PUBMED   39738812
REFERENCE   2  (bases 1 to 11390)
  AUTHORS   Choi,J.E., Qiao,Y., Kryczek,I., Yu,J., Gurkan,J., Bao,Y.,
            Gondal,M., Tien,J.C., Maj,T., Yazdani,S., Parolia,A., Xia,H.,
            Zhou,J., Wei,S., Grove,S., Vatan,L., Lin,H., Li,G., Zheng,Y.,
            Zhang,Y., Cao,X., Su,F., Wang,R., He,T., Cieslik,M., Green,M.D.,
            Zou,W. and Chinnaiyan,A.M.
  TITLE     PIKfyve, expressed by CD11c-positive cells, controls tumor immunity
  JOURNAL   Nat Commun 15 (1), 5487 (2024)
   PUBMED   38942798
  REMARK    GeneRIF: PIKfyve, expressed by CD11c-positive cells, controls tumor
            immunity.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11390)
  AUTHORS   Zhang,X., Li,S., Malik,I., Do,M.H., Ji,L., Chou,C., Shi,W.,
            Capistrano,K.J., Zhang,J., Hsu,T.W., Nixon,B.G., Xu,K., Wang,X.,
            Ballabio,A., Schmidt,L.S., Linehan,W.M. and Li,M.O.
  TITLE     Reprogramming tumour-associated macrophages to outcompete cancer
            cells
  JOURNAL   Nature 619 (7970), 616-623 (2023)
   PUBMED   37380769
REFERENCE   4  (bases 1 to 11390)
  AUTHORS   Kawaguchi,K., Watanabe,M., Furukawa,S., Koga,K., Kanamori,H.,
            Ikemoto,M.J., Takashima,S., Maeda,M., Oh-Hashi,K., Hirata,Y.,
            Furuta,K. and Takemori,H.
  TITLE     Intermittent inhibition of FYVE finger-containing phosphoinositide
            kinase induces melanosome degradation in B16F10 melanoma cells
  JOURNAL   Mol Biol Rep 50 (7), 5917-5930 (2023)
   PUBMED   37248430
  REMARK    GeneRIF: Intermittent inhibition of FYVE finger-containing
            phosphoinositide kinase induces melanosome degradation in B16F10
            melanoma cells.
REFERENCE   5  (bases 1 to 11390)
  AUTHORS   Hung,S.T., Linares,G.R., Chang,W.H., Eoh,Y., Krishnan,G.,
            Mendonca,S., Hong,S., Shi,Y., Santana,M., Kueth,C.,
            Macklin-Isquierdo,S., Perry,S., Duhaime,S., Maios,C., Chang,J.,
            Perez,J., Couto,A., Lai,J., Li,Y., Alworth,S.V., Hendricks,E.,
            Wang,Y., Zlokovic,B.V., Dickman,D.K., Parker,J.A., Zarnescu,D.C.,
            Gao,F.B. and Ichida,J.K.
  TITLE     PIKFYVE inhibition mitigates disease in models of diverse forms of
            ALS
  JOURNAL   Cell 186 (4), 786-802 (2023)
   PUBMED   36754049
REFERENCE   6  (bases 1 to 11390)
  AUTHORS   Ikonomov,O.C., Sbrissa,D. and Shisheva,A.
  TITLE     Mammalian cell morphology and endocytic membrane homeostasis
            require enzymatically active phosphoinositide 5-kinase PIKfyve
  JOURNAL   J Biol Chem 276 (28), 26141-26147 (2001)
   PUBMED   11285266
REFERENCE   7  (bases 1 to 11390)
  AUTHORS   Shisheva,A., Rusin,B., Ikonomov,O.C., DeMarco,C. and Sbrissa,D.
  TITLE     Localization and insulin-regulated relocation of phosphoinositide
            5-kinase PIKfyve in 3T3-L1 adipocytes
  JOURNAL   J Biol Chem 276 (15), 11859-11869 (2001)
   PUBMED   11112776
REFERENCE   8  (bases 1 to 11390)
  AUTHORS   Sbrissa,D., Ikonomov,O.C. and Shisheva,A.
  TITLE     PIKfyve lipid kinase is a protein kinase: downregulation of
            5'-phosphoinositide product formation by autophosphorylation
  JOURNAL   Biochemistry 39 (51), 15980-15989 (2000)
   PUBMED   11123925
REFERENCE   9  (bases 1 to 11390)
  AUTHORS   Sbrissa,D., Ikonomov,O.C. and Shisheva,A.
  TITLE     PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase,
            synthesizes 5-phosphoinositides. Effect of insulin
  JOURNAL   J Biol Chem 274 (31), 21589-21597 (1999)
   PUBMED   10419465
REFERENCE   10 (bases 1 to 11390)
  AUTHORS   Shisheva,A., Sbrissa,D. and Ikonomov,O.
  TITLE     Cloning, characterization, and expression of a novel Zn2+-binding
            FYVE finger-containing phosphoinositide kinase in insulin-sensitive
            cells
  JOURNAL   Mol Cell Biol 19 (1), 623-634 (1999)
   PUBMED   9858586
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY271210.1, AK173077.1 and AC164079.11.
            
            On Jul 16, 2015 this sequence version replaced XM_006495780.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK173077.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                BY271210.1         1-65
            66-2987             AK173077.1         1-2922
            2988-2988           AC164079.11        91580-91580
            2989-6706           AK173077.1         2924-6641
            6707-11390          AC164079.11        119160-123843
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 32.94 cM"
     gene            1..11390
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /note="phosphoinositide kinase, FYVE type zinc finger
                     containing"
                     /db_xref="GeneID:18711"
                     /db_xref="MGI:MGI:1335106"
     exon            1..149
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            150..330
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..6452
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.1.150"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phosphatidylinositol-4-phosphate 5-kinase, type III;
                     phosphoinositide kinase, FYVE finger containing;
                     phosphatidylinositol-3-phosphate/phosphatidylinositol
                     5-kinase, type III; PtdIns(4)P-5-kinase; FYVE
                     finger-containing phosphoinositide kinase;
                     phosphatidylinositol 3-phosphate 5-kinase type III;
                     1-phosphatidylinositol 3-phosphate 5-kinase; PIPkin-III;
                     type III PIP kinase; serine-protein kinase PIKFYVE"
                     /codon_start=1
                     /product="1-phosphatidylinositol 3-phosphate 5-kinase
                     isoform 1"
                     /protein_id="NP_001297553.1"
                     /db_xref="CCDS:CCDS78600.1"
                     /db_xref="GeneID:18711"
                     /db_xref="MGI:MGI:1335106"
                     /translation="MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKS
                     AYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHR
                     RSSVLDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQ
                     CKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIAL
                     SYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLI
                     HPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANR
                     NYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVN
                     WLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSP
                     DSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVD
                     SDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKE
                     SLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLL
                     QNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVN
                     GFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQ
                     RIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQL
                     LTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEIL
                     IFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQD
                     PECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALF
                     ALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEV
                     TSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSP
                     LLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQT
                     LGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPT
                     KVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCF
                     RSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQ
                     VTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVAS
                     FSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTD
                     TFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQ
                     SLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLH
                     NGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLL
                     GSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSS
                     IIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTN
                     RTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLES
                     QGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEE
                     EFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNA
                     VQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNR
                     NVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDY
                     SLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTR
                     FCEAMDKYFLMVPDHWTGLDLNC"
     exon            331..480
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            481..599
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            600..771
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            772..979
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1069
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1208
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1366
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1478
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1626
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1794
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1795..1854
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1984
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2165
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2166..2240
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2241..2348
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2349..2489
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2490..2616
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2617..3773
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            3774..3874
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            3875..3946
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            3947..4118
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4119..4297
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4298..4411
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4412..4520
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4521..4675
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4676..4766
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4767..4827
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4828..4965
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4966..5086
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5087..5182
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5183..5266
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5267..5364
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5365..5529
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5530..5589
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5590..5766
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5767..5870
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5871..5999
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6000..6261
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6262..6336
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6337..11390
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9455..9460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /note="hexamer: AATAAA"
     polyA_site      9495
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /note="major polyA site"
     regulatory      11359..11364
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
                     /note="hexamer: AATAAA"
     polyA_site      11390
                     /gene="Pikfyve"
                     /gene_synonym="5230400C17Rik; p235; Pip5k; Pip5k3;
                     Pipk5k3; PipkIII"
ORIGIN      
        1 agttaccgcg taagcctctg ggcttctcgg cgcgaccgcc ggctgcctga gaaagccggc
       61 gctgtgcttg gcctcggctt cccggccgag agtggtgagg cggaggccgg ggaggcggga
      121 ggcccgcggg gccggggcgg gaggcgaaga ctcatgaaat ggccacagat gacaagagtt
      181 ccccgacact ggactctgct aatgatttgc ctcgctctcc tgccagtcct tctcacctca
      241 ctcactttaa acccttgact cctgaccagg atgagccccc cttcaagtca gcatatagtt
      301 cttttgtaaa cctttttcgt tttaacaaag agcgaggaga agggggccaa ggagagcagc
      361 agtctccgag ttcaagttgg gccagccctc agatcccttc aagaacacag tctgtgaggt
      421 cgcctgtacc ttataaaaaa cagcttaatg aggagctcca ccggcgctct tcagtgttag
      481 actccagaag gaaagcagaa ccagcctgtg gaggtcatga cccacgtaca gctgttcagc
      541 ttcgaagcct cagcacagta ttgaaacgcc tcaaagaaat catggaagga aaaagccagg
      601 acagtgacct gaagcaatat tggatgccag atagccagtg taaagagtgc tatgactgca
      661 gtgaaaagtt tacaacattt aggcgcagac accattgcag actgtgtggg cagattttct
      721 gcagtcgttg ttgtaatcaa gaaatccctg gaaaatttat gggctataca ggagacctcc
      781 gagcatgcac ctactgtaga aaaatagcct taagttatgc tcattctaca gacagtaatt
      841 ccattgggga agacttgaat gctctttcag attcaacttg ctctgtgtct atacttgatc
      901 caagcgaacc tcggacacca gttgggagta gaaaagccag tcgtaacata ttcttagagg
      961 atgatttagc ctggcaaagc ttgattcatc cggattcctc aaatagtgct ctttcaacaa
     1021 gactcgtatc tgttcaagag gatgctggga agtctcctgc tcgaaacaga tcagccagca
     1081 ttactaatct gtcactggat cggtctggtt ctcctatggt tccttcatat gagacatctg
     1141 tcagtcccca ggctaaccga aactacatta ggacagagac gactgaggat gaacgcaaaa
     1201 ttcttctgga cagtgctcag ttaaaggatc tgtggaagaa aatctgccat cacaccagtg
     1261 ggatggaatt tcaagatcac cgttactggt tgagaacaca tcccaactgc attgtaggga
     1321 aggaattagt caactggcta atcagaaatg gacacatcgc tacaagggca caagctatag
     1381 caattggaca agcaatggtt gatggacgtt ggttggattg tgttagtcat catgatcagc
     1441 ttttcaggga cgaatatgcg ttgtatagac cacttcagag tacagaattt tctgagacac
     1501 cttctccaga cagtgactct gtgaactctg tggaaggaca ctccgagcca tcctggttta
     1561 aagacataaa atttgatgac agtgacacag aacagattgc tgaagaaggt gacgataatt
     1621 tggctaattc tgccagtcct agcaagcgca cctcagtcag cagtttccag tccacagtgg
     1681 acagtgactc agccgcttct atcagcctga acgtggagct ggacaacgtg aacttccata
     1741 tcaagaagcc ctccaagtac ccacatgtgc cccctcaccc tgctgaccag aaagagtatt
     1801 tggtttctga cactggagga cagcagctct caataagtga tgccttcatc aaagagtcct
     1861 tatttaatcg acgagtagag gaaaaatcca aagagctgcc ttttacccct ttgggctggc
     1921 atcataacaa cctggaactc ttgcgagagg agaatgagga gaagcaagcc atggaaaggc
     1981 tgctttcagc taatcataac cacatgatgg ccctactcca gcagttgctt caaaacgagt
     2041 cattgtcatc gtcttggagg gacatcattg tgtcactagt ctgccaggtt gttcagacag
     2101 tccgacctga tgtcaagcac caggatgatg acatggatat ccgtcagttt gtccatatca
     2161 agaagatccc aggtggaaag aaatttgact ctgtggttgt caatggcttt gtttgtacca
     2221 agaacattgc acataaaaag atgaattcct gtattaaaaa ccccaaaatc cttctgttga
     2281 agtgttctat tgagtatctc tatagagaag aaactaagtt tacctgcatt gatcctattg
     2341 tgcttcagga aagggaattc ttgaagaatt atgttcaacg aatagttgat gttcgaccca
     2401 cattggttct tgttgagaaa acagtgtctc ggattgctca ggacatgtta ctggaacatg
     2461 gcattactct ggtcattaat gtaaagtcac aagttttaga aagaatcagt cgaatgaccc
     2521 aaggtgattt agtggtgtcc atggaccagc tgctcaccaa accccacttg ggcacttgcc
     2581 acaaatttta tatgcagata tttcagctgc ctaatgaaca aaccaaaaca ctgatgtttt
     2641 ttgaaggctg tccacagcat ctaggctgca caatcaagct cagaggaggc tctgattatg
     2701 agctggctcg agttaaggag atcctaatat ttatgatctg tgtagcttat cattctcagc
     2761 tagaaatctc ttttctcatg gatgagttcg ctatgcctcc aacattaatg caaagccctt
     2821 cattccatct tctgacggag ggacgaggtg aagagggagc ctctcaggag caggtcagtg
     2881 gcagctccct tcctcaggat cctgagtgcc ctcgtgaggc cctgtcttct gaggatagca
     2941 ctttgttgga atcaaggact gtgctagaga agggtgaact agacaataaa agtattccac
     3001 aagctgttgc ctctttgaag catcaagatt ataccacccc cacttgccca gcaggtattc
     3061 cctgtgctct ttttgcattg gtaccagagt cattgttgcc tctccatatg gatcaacagg
     3121 atgccgtagg aaatgaacag ccagagactt cacagcaaac ggatgagcaa caggatccca
     3181 aaagccagat gaaagctttt agagaccctt tacaggatga cactggaatg tacgttactg
     3241 aggaagtcac ctcctctgaa gatcaacgaa agacttatgc cttgacattt aaacaggagt
     3301 taaaagatgt aatcctctgt atctctccag ttattacatt ccgtgaacct ttccttttaa
     3361 ctgaaaaggg gatgagatgc tcaactcgag attattttcc agagcagatt tactggtctc
     3421 ctcttctcaa caaagaggtg aaggaaatgg agagcaggag gaagaaacag ctgctcaggg
     3481 atctctctgg acttcagggc atgaatggca gtgttcaggc caagtctatt caagtcttac
     3541 cctcacatga gctagtgagc accaggattg ctgaacatct gggtgacagc cagaccttgg
     3601 gtagaatgct agctgattat cgagctagag gaggaagaat tcagtcaaaa catttggacc
     3661 cctttgtcca ttcaaaagat gcatcatgta cttcaggtgg caaatcagga aacaaaactg
     3721 agagtgatga agagagggga ttgattccaa gtgatgtaat atggccaaca aaggtggact
     3781 gtctgaaccc tgctaaccac cagaggctct gtgtgctctt cagcagctct tctgcccagt
     3841 ccagcaatgc tcccagtgct tgtgtcagtc cttggattgt aacaatggag ttttatggaa
     3901 agaatgacct tacactgggg atatttttag aaagatactg tttcaggtct tcttaccagt
     3961 gtccgagcat gttctgtgac acccccatgg ttcatcacat tcgacgcttt gttcatggcc
     4021 aaggctgtgt acagataatt ctgaaggagt tggattctcc agtgcctgga tatcaacata
     4081 caattctcac atattcctgg tgcagaatct gcaaacaagt aacaccagtt gttgctcttt
     4141 caaatgaatc ctggtctatg tcatttgcaa agtaccttga acttcgattt tatggccacc
     4201 agtacacacg cagagccaac gctgagccct gcggtcactc tatccaccat gattatcacc
     4261 agtatttctc ttataaccag atggtggcat ctttcagtta ctctcctatt cggcttcttg
     4321 aagtatgtgt tccactacca aaaatattca ttaagcgtca agccccactg aaggtatctc
     4381 ttcttcagga cctcaaagac ttttttcaga aggtttcaca ggtgtaccta gctgttgatg
     4441 agagacttgc atccttgaaa acggatacat ttagcaaaac tagagaggaa aagatggaag
     4501 atatctttgc acaaaaggag atggaagagg gtgagtttaa gaactggaca gagaagatgc
     4561 aagcaaggct catgtcttcc tctgtggata cccctcagca actgcagtcc atttttgagt
     4621 cactgattgc caagaagcaa agcctctgtg aggtgctcca ggcgtggaac agcaggttgc
     4681 aggacctctt ccagcaggaa aaaggtagaa agaggccttc agttcctccc agtcctggga
     4741 gactgagaca aggtgaagaa agcaagataa atgcaatgga cacatctcca aggaatattt
     4801 ctccaggact tcacaatgga gaaaaagagg atcgcttctt gacaaccctg tccagccaga
     4861 gctctacgag ctccacccac ctccagctgc ccactcctcc cgaggccctg gccgagcagg
     4921 tagtgggagg gccgactgat ctggattcag ccagtggctc tgaagatgta tttgatggtc
     4981 atttgctggg atccacagac agccaggtga aggaaaagtc aaccatgaaa gccatctttg
     5041 ctaatttgct tccaggaaac agctacaatc ccattccatt tccttttgat ccagataaac
     5101 actacttaat gtatgaacat gaacgggtgc ccattgctgt ctgtgagaaa gagcccagct
     5161 ccatcattgc ttttgcactc agttgtaaag aataccgcaa tgccttagag gaattgtcca
     5221 aagcaactct gcggaacagt gctgaagaag ggctcccagc caatagtgct ttagataaca
     5281 gacctaagag tagcagccct attagactac ctgaaatcag tggaggacag acaaaccgca
     5341 cagtagaagc agaacctcag ccaaccaaaa aagcttcagg aatgttgtcc ttcttcagag
     5401 gaacagcagg gaagagccct gatctgtctt cccagaagag ggagaccttg cgaggggcag
     5461 acagtgctta ctaccaggtt gggcaggccg gcaaggaggg gttggagagt caaggcctgg
     5521 agcctcaaga tgaagtagat ggaggagata cacagaagaa acaactcaca aatcctcatg
     5581 tggaacttca attttctgat gctaatgcca agttttactg tcggctgtac tacgcgggag
     5641 agttccacaa gatgcgtgaa gtgattctgg gcagcagtga ggaggaattc atccgttccc
     5701 tttctcactc atctccctgg caggcccggg gaggcaagtc aggagctgct ttctatgcca
     5761 ccgaagatga tagattcatt ctgaagcaaa tgcctcgttt ggaagtccag tctttccttg
     5821 actttgcacc acactacttc aattatatca caaatgctgt tcaacaaaag aggcccaccg
     5881 ccttggctaa aattcttgga gtttacagaa ttggttataa gaactctcag aacaacactg
     5941 agaagaagtt agatctcctt gtcatggaaa atcttttcta tgggagaaag atggcacagg
     6001 tttttgattt gaagggttca cttaggaatc gaaatgtaaa aactgacact gggaaagaga
     6061 gctgtgatgt ggttctgttg gatgaaaacc tcctaaagat ggttcgagac aaccctctct
     6121 atattcgttc ccattccaaa tctgtgctga gaacctccat ccacagcgac gcccatttcc
     6181 tttccagcca cctcattata gactattctc tgctggttgg gcgagatgac actagcaatg
     6241 agcttgtggt tggcatcata gattacattc gaacatttac atgggacaaa aaacttgaga
     6301 tggttgtgaa gtcaacagga attttaggag gacaaggtaa aatgccaact gtggtctctc
     6361 cagagttgta taggactaga ttttgtgaag caatggacaa gtatttcttg atggtgccag
     6421 accactggac agggttggat ctgaattgct gaaatcacac agatgatttg aaaaagactg
     6481 tgaatgaaag tggatctgaa gaagaaaccc aaactctatg atgttttgtt tctttgtcat
     6541 atataccact gaacacaaag acctttcagt tctgtgactg tctatagatt ggctttagac
     6601 tgtccatgac tgcagggtaa aaaagcccca tggatttgct tttgatattt gctattatct
     6661 gcctgtaaat gggaaattgt aaaagttaaa tacaatgttt tttttcaaag ggctgtgatt
     6721 agagatgact atatagatgg agtaagatat tgagttgtca tacttactaa ctcaattgct
     6781 tgaagaggaa agcgctgtct gatagacact gtcttgtaga ctgacaggta gactgtctag
     6841 tagactgtga ctgtctagta gactatgact gtcgggcatc acatttgtta cttttttcca
     6901 tgagaaggca cttgcagacc ttcttggttt ggtatacgtt tcctgtgtag tgactttatg
     6961 tttgttctta taatatctaa aacatttagg aaaaatctga tttattccat taatggccag
     7021 ttaaacagat tggtgcttgc tttattagga atttgactta aattaggaat cctgccataa
     7081 tctatattct ccaactgttt tgatgctttc acattctacc ttctatgcag gtgacaagag
     7141 cctttattcc ttagcttgag agcctttctt agaacatttt aaggagcttg tttaaaacat
     7201 ttgagtaatg tgccaaacag cacttaatct gcatatgtgt tcatagtcta gatttacctc
     7261 tcattttggg caagcaaaat aagcaggaaa tgaaactgta gtcactgaag ccctgagtga
     7321 agaattaata atgaaagctt tttttttaac ggcaaagaga aactaattga gaatcaaaaa
     7381 tgtggggttt tgttgtttgt ttttgttttt cttatatatg tatttgtaaa gtatctttta
     7441 acactactag cagttaggtg ggatttgtaa aatttaaaag ttttcagtat ggatactctc
     7501 attttaagtt tccatcacac tgaccagtgc ttctgagaaa tgctgagagg taaagttact
     7561 cctactgaag gagtagttta ctagttagca gtatcatcta aaacatgacc agaaatcctg
     7621 aactgtgagc agatgctttc accccataat atgcgagtgt ttctcttgcc ttttctgtaa
     7681 caaggatatt tggaaattta taaagttgta agatttccat tttattcaga ctttatctaa
     7741 tagcatgtta atattcacaa agggctggat ggacgatgct acagagggca gtctgcaccc
     7801 gagtcagcac agtgtggcag gtttgctgta gacagtgaag cagtagctgg ctattggtta
     7861 cttgttgagt gtgtggctaa acaaggctta tgtatgcttg atgttaggcc ttggcctctt
     7921 cagtgccttg gaaagatgga ccgaacactg aagagtccag ctgtgatgtt aaagatcagt
     7981 aaaggaatgt atagatttga agattgtgtg gaacacactt tctgtgcttc ctgggacaaa
     8041 ggcttttgtg tctggtttga aatgccagct gatttgtctt tgttcatttt ttctaatctt
     8101 gattgattag gctataggac cctctcactt cctgtaggac tagagagata ccttgcctag
     8161 ctggggttgt gttgtttctg tttttctctt tggtttcagt ccagtgcata tttgagctga
     8221 gctccagggg ggtaactcct gacagtaaca tggcgtgtcc atcttctctt gtccctgatt
     8281 gaaaagcaac tttgcttttt aaagtattgt ttaaagtaag caaatattga ggtattttgg
     8341 tgactttctg aagcaagagt ttgtactttg aaagcttgat ttgttttttt gctttttttt
     8401 tttttgtttt gttttgtttt tttaaattaa tgtctggctg gtgttaatga aggggaaaca
     8461 cataattgaa ctgttttgcg tggtacaaat gtgtgactcc tggctgcttg tgtttagcaa
     8521 ctgctgtgta tatgtgtgta tatatagtgt tctcagtgct ttgaattgaa tggctgttgt
     8581 cctccttgac tccctttcat ggcctagaga atattaatgc tgaaaagctg tgaatacgaa
     8641 agaaatctca tgctcaaaat atgaattcca aagtagctga tagtaacatg agaaaattat
     8701 gcctacgtgt tgctgtgtat aaaaatcttg ttcacaaata tgaaactttt tgatgacgta
     8761 aaattttgtt taaagactag aatttgaatt tttcatgtct gcctgagaaa agaaagctgg
     8821 aataagctgg agagcccttt tgtagtctat tgagtgctta tagattctca agatttccta
     8881 gggaaggaca gagcaggggt tttctagctt tgacctctat gtatgtatgg ggtcactctt
     8941 gagcattggc ttgaggtgac tgtgtggtgg atggcttctt ggcacgttag ccatggcact
     9001 gggcttctgt agaagtgcca aggaggtgat ctgcctgtgg caagtcatct aagtttcaag
     9061 tgtgaaaact attgtgagcc tgggaccgag tcttcctagg acacccagtt tctaaagtga
     9121 cttacgcagt attcaaacct tctgtgcaat gccttttttt ggttgttttt gtaacgggtg
     9181 cagtgtatta taggaaaaaa aaggaaacta caatcacaag cagttttgtt taatgctgct
     9241 ttgtcagttt tgtaaaagaa aatgtacata atgtctttca acaggtttta gactagagga
     9301 aataacattg taaatcacag tagcctaatt taatagtaaa agtacttaat gtattataat
     9361 gtgtatattt ctctaacttt gtttccagct gttttatatg attgacatgt tatttaaaga
     9421 taactaccat agcttcttag agacttgtac tgtaaataaa gaggaccttc aataaactgg
     9481 aatttactta ctctactaga tttcagatct ctttgaggaa attaacaaag tgagagaaag
     9541 ttgaactcaa ctctgttgac cagactggct tcaaactcac agaaaagcac cttcccctat
     9601 gagtactggg attaaaggca tgttccacca ccgcccggcc ctcagtcttg aatgactagt
     9661 aatgcccatg gctgtatagg ccttccaatg tttagttttc ttgataaagc atccagggaa
     9721 gtgaatccct tttctgtaca aaataatctg gttgtgtggg tggatggtac tccatcattc
     9781 attattttct cctatcaact tgaagacaaa tactgtactg gctgaaactg aaaccatctt
     9841 ggtggtactc atggttgtgg tctctaggcc acgaggccca ctttccctta gattcatgag
     9901 ggcaaagctg tgaggggggg agaggagaga gaggagaaga gagaggagag aggggggaga
     9961 ggagagagag gagagagagg ggagagagaa gagaggggag agaggagaaa gaagctaggt
    10021 gggaaggttt gtagacattg tcccaagctt ctgactttca ggctagactc ctcccagtta
    10081 cctagtaaca acaagataac aagcccacta taagaggggc tgctcggccc ctccttgctc
    10141 tcttactccc tttactctct tacctcttac tctaacctct tactctctag cctttcctct
    10201 cgctctcctt ttccccttct ctcctcttgg acatggctgg tttctccctc tctttctttc
    10261 caccttctct cctttctccc tgcctttcta caataaagct ctaaaaccat agactgtctc
    10321 tgttcatcaa ggcccactgt gcttggatga tcagataggt tttcttctaa ccagctgtgc
    10381 ctaatctccc atctcctgtc agaaggcctt cctgtgctcc agccacagac tggactaagg
    10441 actcttgcct gcatgggaac ctctctccct ctgccctctc ttcttgtatc tcctggccga
    10501 gagtgttgcc ccggggcccc tggactgggg gctgcccctt gtccaccctc tgcagtgtgc
    10561 ggtcagtggc tccatacaga cagatgccca cctgaggccg agtggaaagc gtccggcaga
    10621 gttcctgtcc tcccgcatcc gcctgcccag agcacaggaa ctctggctgg aagcgggctc
    10681 tctcccccct acccccatac ccacagccac acactgagtg tgccccagag atggctatgg
    10741 ctaggttatg tggggaagta gggaagttac agacttaaag atgggcacct ttgctcttgt
    10801 aggatggaag aagtgaatgg taggaagaga attcctaagt caatgcttaa ggaaattaag
    10861 aaatttttaa ttttatctta gcagttatat gctatagtca gatacataga gcaaaattta
    10921 ccatctaaaa tttttaagtt aaaattcagt ggtgtgaagc acactcttat cttcaaaatc
    10981 ccaggtaact accatctgat ttgtaagaat gtgattatcc aagttacctc atataaatga
    11041 ttttgtgact ctcctgtttc acttacatat tttatgttat gacaagtgtt acctgcttaa
    11101 tatttgatac atcctctatt caaaatacta tattaaaagg agttttggag ctgggagatg
    11161 ggataggagc ttgttctgtc cactccacac actggttgga tgttatcaca actgtcagac
    11221 cccatttggg ggacgtggag ttgacttcgt agctaatgct ttgggggatt ttggttttgg
    11281 tgttgggagt ctgtttccaa agtgatgtaa tttgtaagta ctgaaagttt aggcttccag
    11341 catttgaaga cgagatataa taaatcagct ctacagtctt gttctcttta
//
