LOCUS       NM_001081309            4884 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus phosphoinositide-3-kinase regulatory subunit 4
            (Pik3r4), mRNA.
ACCESSION   NM_001081309 XM_889806
VERSION     NM_001081309.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Alkhoury,C., Henneman,N.F., Petrenko,V., Shibayama,Y., Segaloni,A.,
            Gadault,A., Nemazanyy,I., Le Guillou,E., Wolide,A.D.,
            Antoniadou,K., Tong,X., Tamaru,T., Ozawa,T., Girard,M., Hnia,K.,
            Lutter,D., Dibner,C. and Panasyuk,G.
  TITLE     Class 3 PI3K coactivates the circadian clock to promote rhythmic de
            novo purine synthesis
  JOURNAL   Nat Cell Biol 25 (7), 975-988 (2023)
   PUBMED   37414850
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Lei,Y., Zhang,X., Xu,Q., Liu,S., Li,C., Jiang,H., Lin,H., Kong,E.,
            Liu,J., Qi,S., Li,H., Xu,W. and Lu,K.
  TITLE     Autophagic elimination of ribosomes during spermiogenesis provides
            energy for flagellar motility
  JOURNAL   Dev Cell 56 (16), 2313-2328 (2021)
   PUBMED   34428398
REFERENCE   3  (bases 1 to 4884)
  AUTHORS   Iershov,A., Nemazanyy,I., Alkhoury,C., Girard,M., Barth,E.,
            Cagnard,N., Montagner,A., Chretien,D., Rugarli,E.I., Guillou,H.,
            Pende,M. and Panasyuk,G.
  TITLE     The class 3 PI3K coordinates autophagy and mitochondrial lipid
            catabolism by controlling nuclear receptor PPARalpha
  JOURNAL   Nat Commun 10 (1), 1566 (2019)
   PUBMED   30952952
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4884)
  AUTHORS   Gstrein,T., Edwards,A., Pristoupilova,A., Leca,I., Breuss,M.,
            Pilat-Carotta,S., Hansen,A.H., Tripathy,R., Traunbauer,A.K.,
            Hochstoeger,T., Rosoklija,G., Repic,M., Landler,L., Stranecky,V.,
            Durnberger,G., Keane,T.M., Zuber,J., Adams,D.J., Flint,J.,
            Honzik,T., Gut,M., Beltran,S., Mechtler,K., Sherr,E., Kmoch,S.,
            Gut,I. and Keays,D.A.
  TITLE     Mutations in Vps15 perturb neuronal migration in mice and are
            associated with neurodevelopmental disease in humans
  JOURNAL   Nat Neurosci 21 (2), 207-217 (2018)
   PUBMED   29311744
  REMARK    GeneRIF: This study demonstrated that Mutations in Vps15 perturb
            neuronal migration in mice.
            Erratum:[Nat Neurosci. 2018 Aug;21(8):1139. doi:
            10.1038/s41593-018-0170-9.. PMID: 29875394]
REFERENCE   5  (bases 1 to 4884)
  AUTHORS   Nemazanyy,I., Montagnac,G., Russell,R.C., Morzyglod,L.,
            Burnol,A.F., Guan,K.L., Pende,M. and Panasyuk,G.
  TITLE     Class III PI3K regulates organismal glucose homeostasis by
            providing negative feedback on hepatic insulin signalling
  JOURNAL   Nat Commun 6, 8283 (2015)
   PUBMED   26387534
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4884)
  AUTHORS   Pampliega,O., Orhon,I., Patel,B., Sridhar,S., Diaz-Carretero,A.,
            Beau,I., Codogno,P., Satir,B.H., Satir,P. and Cuervo,A.M.
  TITLE     Functional interaction between autophagy and ciliogenesis
  JOURNAL   Nature 502 (7470), 194-200 (2013)
   PUBMED   24089209
REFERENCE   7  (bases 1 to 4884)
  AUTHORS   Nemazanyy,I., Blaauw,B., Paolini,C., Caillaud,C., Protasi,F.,
            Mueller,A., Proikas-Cezanne,T., Russell,R.C., Guan,K.L.,
            Nishino,I., Sandri,M., Pende,M. and Panasyuk,G.
  TITLE     Defects of Vps15 in skeletal muscles lead to autophagic vacuolar
            myopathy and lysosomal disease
  JOURNAL   EMBO Mol Med 5 (6), 870-890 (2013)
   PUBMED   23630012
  REMARK    GeneRIF: Vps15-deficient mouse tissues are competent for
            LC3-positive autophagosome formation and maintain mTOR activation.
REFERENCE   8  (bases 1 to 4884)
  AUTHORS   Kim,J., Kim,Y.C., Fang,C., Russell,R.C., Kim,J.H., Fan,W., Liu,R.,
            Zhong,Q. and Guan,K.L.
  TITLE     Differential regulation of distinct Vps34 complexes by AMPK in
            nutrient stress and autophagy
  JOURNAL   Cell 152 (1-2), 290-303 (2013)
   PUBMED   23332761
REFERENCE   9  (bases 1 to 4884)
  AUTHORS   Lindmo,K. and Stenmark,H.
  TITLE     Regulation of membrane traffic by phosphoinositide 3-kinases
  JOURNAL   J Cell Sci 119 (Pt 4), 605-614 (2006)
   PUBMED   16467569
  REMARK    Review article
REFERENCE   10 (bases 1 to 4884)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC157514.2.
            
            On Feb 6, 2007 this sequence version replaced XM_889806.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC157947.1, SRR7974084.18190.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-396               AC157514.2         115026-115421       c
            397-1170            AC157514.2         113447-114220       c
            1171-1304           AC157514.2         109617-109750       c
            1305-1887           AC157514.2         107517-108099       c
            1888-2022           AC157514.2         104330-104464       c
            2023-2244           AC157514.2         103379-103600       c
            2245-2418           AC157514.2         99824-99997         c
            2419-2564           AC157514.2         96934-97079         c
            2565-2768           AC157514.2         95171-95374         c
            2769-2970           AC157514.2         90483-90684         c
            2971-3158           AC157514.2         89243-89430         c
            3159-3369           AC157514.2         88460-88670         c
            3370-3535           AC157514.2         85607-85772         c
            3536-3700           AC157514.2         81509-81673         c
            3701-3912           AC157514.2         80096-80307         c
            3913-4044           AC157514.2         76100-76231         c
            4045-4145           AC157514.2         73185-73285         c
            4146-4234           AC157514.2         72194-72282         c
            4235-4343           AC157514.2         71962-72070         c
            4344-4884           AC157514.2         70761-71301         c
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 56.79 cM"
     gene            1..4884
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /note="phosphoinositide-3-kinase regulatory subunit 4"
                     /db_xref="GeneID:75669"
                     /db_xref="MGI:MGI:1922919"
     exon            1..396
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            397..1170
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     CDS             438..4514
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /EC_number="2.7.11.1"
                     /note="PI3-kinase regulatory subunit 4;
                     phosphatidylinositol 3 kinase, regulatory subunit,
                     polypeptide 4, p150"
                     /codon_start=1
                     /product="phosphoinositide 3-kinase regulatory subunit 4"
                     /protein_id="NP_001074778.1"
                     /db_xref="CCDS:CCDS40753.1"
                     /db_xref="GeneID:75669"
                     /db_xref="MGI:MGI:1922919"
                     /translation="MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAK
                     HREGLVVVKVFAIQDPTLPLTSYKQELEELKIRLHSAQNCLPFQKAAEKASEKAAMLF
                     RQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTSWN
                     WVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDP
                     STPLVDLNSNQRARGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQ
                     VLNKIEDRSIRDLVTQMINREPEKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKE
                     TFLSADERILVIRKDLGNIIHNLCGHDLPEKAEGESRASGLVVLVSVITSCLQTLKSC
                     DSKLAALELILHLAPRLSVEILLDRITPYLLHFSNDSVPRVRAEALRTLTKVLALVQE
                     VPRNDVNIYPEYILPGIAHLAQDDATIVRLAYAENIALLAETALRFLELVQLKTLNME
                     NEPDNEEVDEATRPNGDYDTELQALHEMVQQKVVTLLSDPENIVKQTLMESGITRLCV
                     FFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGL
                     SDAEEFVIVKALNALTCMCQLGLLQKPHVYEFASDIAPFLCHPNLWIRYGAVGFITVV
                     AHQISTADVYCKLMPYLDPYITQPVIQIERKLVLLSVLKEPVSRSIFDYALRSKDIAS
                     LFRHLHMRQKKRNGSLLDCPPPEDPTIAQLLKKLLSQGMTEEEEDKLLALKDFMMKSN
                     RAKANAVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEPDEKRARKHVKQDSNVN
                     EEWKSMFGSLEPPNIPQALPKTSDHEVVQPGKPPRSESSAGICVPLSTSPQVSEAAHI
                     PSKKPVIPVVSSTVLPSTYQIRITTCKTELQQLIQQKREQCNAERIAKQMMENAEWES
                     KPPPPGWRPKGLLVAHLHEHKSAVNRIRVSDEHLLFATCSNDGTVKIWNSQKMEGKTT
                     TTRSILTYSRIGGRVKTLTFCQGSHYLAIASDNGAVQLLGIEASKLPKSPKIHPLQSR
                     ILDQKEDGCVVDMHHFNSGAQSVLAYATVNGSLVGWDLRSSSNAWTLKHDLKSGLITS
                     FAVDIHQCWLCIGTSSGAMACWDMRFQLPISSHCHPSRARIRRLSMHPLYQSWVIAAV
                     QGNNEVSMWDMETGDRRLTLWASSAPPLSELQPSPHSVHGIYCSPADGNPILLTAGSD
                     MKIRFWDLVSPERSYVVAGSTGSPSVSYYKKIIEGTEVVQEIQNKQKVGPSDDTPRRG
                     PESLPVGHHDIITDIATFQTTQGFIVTASRDGIVKVWK"
     exon            1171..1304
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1887
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..2022
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2244
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            2245..2418
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2564
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2768
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            2769..2970
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            2971..3158
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            3159..3369
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            3370..3535
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            3536..3700
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            3701..3912
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            3913..4044
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            4045..4145
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            4146..4234
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            4235..4343
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     exon            4344..4884
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4859..4864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /note="hexamer: AGTAAA"
     polyA_site      4878
                     /gene="Pik3r4"
                     /gene_synonym="2210010O15Rik; C730038E05Rik; D9Ertd418e;
                     p150; Vps15"
                     /note="major polyA site"
ORIGIN      
        1 gaggctttcc cgggagttca gcgtttgccg caggtgaagt cgtcttcctg gacgggcact
       61 gtccctgtcc ctgtccctgt ccagggctgc tgcaaaggcc ctgggatccg gggactcgaa
      121 cccgcggggg cccagggcgg caggggagga gccggcagcg aggctgctgg gtgtccggag
      181 tcccgagtgt gtttgctctc ccagttgttc acccccagct ctagatctcg gacgtgtctg
      241 cgctcctgcc ccaactaccc cggtcccgtg tctgaaagaa gacccctcac agaaaacatt
      301 ctcctggccg gcttccgttc acgcccaagc ccctcggagc tctgccaaga cctccctggg
      361 atctttcagc gacttttgag ttgccagagg cctggggtgt tctgtgttaa gctgctgtcc
      421 cagaggtgct gggcattatg ggaaatcagc tggctggcat cgctccttcc cagatcctgt
      481 ctgtagagag ttatttctca gacatccatg actttgagta tgataagagc ctgggaagca
      541 ctcggttttt taaagtcgct cgagccaagc accgggaagg cctggtggtt gtgaaggtct
      601 ttgcaattca ggatcccacg ttacccttaa ctagttataa acaggagctg gaggaactga
      661 aaatcaggct ccactccgcc cagaactgcc tccccttcca gaaagcagca gaaaaggcgt
      721 ctgagaaagc agccatgctg ttccggcagt atgtgagaga caacctctac gatcgcatca
      781 gtacccgccc gttcctaaac aacatcgaga agcgctggat cgccttccag atcctgacgg
      841 ccgtggacca agcgcacaaa tctggagtcc gccacggaga catcaagaca gagaatgtga
      901 tggtcaccag ttggaactgg gtcctcctaa ccgacttcgc cagttttaag cccacgtatc
      961 ttcccgagga caacccagcg gatttcaact atttctttga cacctcgaga aggaggacat
     1021 gctacatagc tcccgagcgc ttcgttgacg gtgggatgtt cgccaccgag ttagagtaca
     1081 tgagagaccc ctcaaccccg cttgtcgacc tgaatagcaa tcagagagcg cggggagagc
     1141 tgaagagagc catggacatc ttttcggcag gttgcgtgat agctgagctc tttacggaag
     1201 gcgtaccttt gtttgatctc tctcagctgc tggcgtatag aaacggacat tttttccctg
     1261 aacaagtgct aaacaaaatt gaagatcgca gtatcagaga cttggtaact cagatgatta
     1321 accgtgagcc cgagaagcgt ttggaagctg aggactacct gaagcagcag cgaggcaacg
     1381 ccttccctga gatattttat actttccttc agccttacat ggcccagttc gccaaggaaa
     1441 cctttctctc tgcagatgag cggattttgg ttatacggaa agatttgggc aacattattc
     1501 acaacctctg tggacatgat ttgccagaaa aagcagaagg ggagtccagg gccagtggtc
     1561 tggttgtcct ggtgtccgtg ataacgtcct gcctgcagac actgaagtcc tgcgactcca
     1621 aactggccgc cttggagctc atcctgcatc tggcccccag actgagcgtc gagatccttc
     1681 tggatcgcat cactccctac ctgttgcatt tcagcaatga ctctgtccca agagtgcggg
     1741 ctgaagccct gaggacactc accaaagtcc ttgcccttgt ccaagaggtt cctcgcaacg
     1801 atgtcaatat ctatccagag tatattctcc ccggcatagc ccacctggcc caggatgacg
     1861 ctaccatcgt cagactggcc tacgctgaaa acatagctct gttggccgag acggctctgc
     1921 gattcctgga gcttgtgcag ctgaaaactc tcaacatgga gaacgagccc gataacgagg
     1981 aggtagatga agccacgcgt cctaacggag actatgacac agagctccag gccttgcatg
     2041 agatggtcca gcagaaggtg gtcacgttgc taagcgaccc tgagaatatc gtgaaacaga
     2101 cgctgatgga gagtgggatc acacgcttgt gcgtcttctt cggacgccag aaggccaacg
     2161 acgttctctt gtcccacatg atcactttcc tcaatgataa gaatgattgg cacctgcgtg
     2221 gagctttctt tgatagcata gtcggtgtgg ctgcctacgt tggctggcag agctcctcta
     2281 tcctcaagcc cctgctccag caaggcctca gcgatgctga ggagtttgtc atcgtgaaag
     2341 ctctcaatgc cctgacctgc atgtgccagc tagggctgct gcagaaaccc catgtctacg
     2401 agttcgccag tgatattgct cccttcctgt gtcaccctaa cctgtggata cgctatggcg
     2461 ctgtgggatt tatcacggtg gtagctcatc agatcagtac tgctgatgtc tactgcaagc
     2521 tcatgccgta tcttgacccg tatattacac agccagtgat acagattgag aggaagctgg
     2581 tcctgctcag tgtgctaaag gagccggtga gccgctccat atttgactat gctctgaggt
     2641 cgaaagacat cgccagcttg ttcagacatc tccacatgcg ccagaagaag cgaaatggct
     2701 ctcttctcga ctgccctcca cctgaggacc ccaccatagc gcaacttctg aagaagttgc
     2761 tctcacaggg gatgactgaa gaagaggaag ataaacttct ggcactgaag gacttcatga
     2821 tgaaatcaaa tcgagccaaa gctaatgcgg tagaccagag ccacctccac gacagcagcc
     2881 agaaaggtgt aatcgacttg gccgccttag gcatcactgg gagacaagtt gatcttgtta
     2941 aaaccaaaca agaaccagat gagaaacgtg ctaggaaaca tgtgaaacaa gactccaatg
     3001 taaatgaaga gtggaaaagc atgtttgggt cattggagcc accgaatatc ccacaggccc
     3061 tgcctaaaac cagtgaccat gaggttgttc agcctgggaa gccacctcgc tctgagtcct
     3121 ctgctggtat ctgtgtccct ttgtcaacgt ctccacaggt ctcagaagca gctcacatcc
     3181 caagtaagaa gcctgtgatc ccggttgtga gtagcacggt cctgccgtcc acttaccaga
     3241 tccgcatcac cacgtgcaag actgaacttc agcagctcat acagcagaag agggagcagt
     3301 gcaacgcgga gcgcatagcc aagcagatga tggagaatgc cgagtgggag agcaagccac
     3361 ctccacctgg gtggcgtcct aaagggctgc tagttgcaca tcttcatgag cacaaatctg
     3421 ctgtgaatcg catcagagtc tctgatgaac acttactttt tgcaacatgt tcaaatgatg
     3481 gcacagtgaa gatctggaac agccagaaga tggaggggaa gaccaccaca acgcggtcta
     3541 ttctcacgta cagccgaatt ggaggacgag tcaagacgct aacgttttgc caaggctccc
     3601 actacttggc catagcatct gataatggtg ctgtccagct tcttggaatt gaggcgtcta
     3661 agttacccaa gtctcctaaa attcaccctc tacaaagcag gattctggat cagaaggaag
     3721 atggctgtgt ggtggacatg catcacttca actccggggc acagtccgtt cttgcctatg
     3781 ccacggtgaa tggctctctg gttggatggg atctcaggtc ttcaagcaac gcgtggacat
     3841 taaagcatga cctaaagtca ggcctcatca cttcctttgc cgtggacatc caccagtgct
     3901 ggctgtgcat aggcacgagc agcggtgcca tggcgtgttg ggacatgagg ttccagttgc
     3961 ccatctccag tcactgtcat ccctccagag ctcgcatccg gcgcctctcc atgcaccccc
     4021 tgtaccagtc ctgggtaatc gcagctgttc agggcaacaa tgaagtctcc atgtgggaca
     4081 tggaaactgg cgacaggaga ctgactctct gggccagcag cgcaccgcca ctgtctgaat
     4141 tacagccttc accgcacagc gtccatggca tctactgcag ccctgcagat ggaaacccta
     4201 tcctgctgac cgctggctca gacatgaaaa taaggttctg ggacttggtt tccccagaga
     4261 ggtcctatgt tgttgcggga agtacaggtt ccccgtctgt ctcctactac aagaagataa
     4321 tagaaggcac cgaggtcgtc caggaaattc agaataagca gaaggttgga ccgagtgatg
     4381 atacccctcg gaggggcccg gagtctctgc ctgtgggaca tcatgacatc atcacagaca
     4441 ttgccacctt ccagaccact cagggcttca ttgtgactgc ttctagagac ggaattgtga
     4501 aggtgtggaa ataagtcctg ccgacttgta taaaatgctg taaaattcga aatgtaccat
     4561 agctcaggac acgaaggact tctagagaac aaacgtgggt tttgaagtga ctgttactgt
     4621 ttcatggctg agcaaatccc agccacagtg gggaaggatg taggcatggt gactttcttc
     4681 atgtgcatct ctgaagaact ggccagatag cacaacctgg gagcgcttgc tgcatatgga
     4741 acagaacagg tgagaagtgt aaaaagggaa gatgacccgg ccagtttatt ttctatggaa
     4801 atgattgtaa gccgttatgt aaacacactt ttgttattat aaatgctatt caaactcaag
     4861 taaactctta ttcaagcatc ctga
//
