LOCUS       NM_008839               6987 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus phosphatidylinositol-4,5-bisphosphate 3-kinase
            catalytic subunit alpha (Pik3ca), mRNA.
ACCESSION   NM_008839 XM_006535409
VERSION     NM_008839.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Briones,A.C., Del Estal,L., Villa-Gomez,C., Bermejo,V., Cervera,I.,
            Gutierrez-Huerta,P., Montes-Casado,M., Ortega,S., Barbacid,M.,
            Rojo,J.M. and Portoles,P.
  TITLE     T Cell-Specific Inactivation of the PI3K p110alpha Catalytic
            Subunit: Effect in T Cell Differentiation and Antigen-Specific
            Responses
  JOURNAL   Int J Mol Sci 26 (2), 595 (2025)
   PUBMED   39859310
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Huber,A., Allam,A.H., Dijkstra,C., Thiem,S., Huynh,J., Poh,A.R.,
            Konecnik,J., Jacob,S.P., Busuttil,R., Liao,Y., Chisanga,D., Shi,W.,
            Alorro,M.G., Forrow,S., Tauriello,D.V.F., Batlle,E.,
            Boussioutas,A., Williams,D.S., Buchert,M., Ernst,M. and
            Eissmann,M.F.
  TITLE     Mutant TP53 switches therapeutic vulnerability during gastric
            cancer progression within interleukin-6 family cytokines
  JOURNAL   Cell Rep 43 (8), 114616 (2024)
   PUBMED   39128004
REFERENCE   3  (bases 1 to 6987)
  AUTHORS   Conduit,S.E., Pearce,W., Bhamra,A., Bilanges,B., Bozal-Basterra,L.,
            Foukas,L.C., Cobbaut,M., Castillo,S.D., Danesh,M.A., Adil,M.,
            Carracedo,A., Graupera,M., McDonald,N.Q., Parker,P.J.,
            Cutillas,P.R., Surinova,S. and Vanhaesebroeck,B.
  TITLE     A class I PI3K signalling network regulates primary cilia
            disassembly in normal physiology and disease
  JOURNAL   Nat Commun 15 (1), 7181 (2024)
   PUBMED   39168978
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6987)
  AUTHORS   Yamaguchi,J., Isnard,P., Robil,N., de la Grange,P., Hoguin,C.,
            Schmitt,A., Hummel,A., Megret,J., Goudin,N., Luka,M., Menager,M.M.,
            Masson,C., Zarhrate,M., Bole-Feysot,C., Janiszewska,M., Polyak,K.,
            Dairou,J., Baldassari,S., Baulac,S., Broissand,C., Legendre,C.,
            Terzi,F. and Canaud,G.
  TITLE     PIK3CA inhibition in models of proliferative glomerulonephritis and
            lupus nephritis
  JOURNAL   J Clin Invest 134 (15), e176402 (2024)
   PUBMED   38842935
  REMARK    GeneRIF: PIK3CA inhibition in models of proliferative
            glomerulonephritis and lupus nephritis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6987)
  AUTHORS   Becattini,B., Molinaro,A., Henricsson,M., Boren,J. and Solinas,G.
  TITLE     Adipocyte PI3K links adipostasis with baseline insulin secretion at
            fasting through an adipoincretin effect
  JOURNAL   Cell Rep 43 (5), 114132 (2024)
   PUBMED   38656871
REFERENCE   6  (bases 1 to 6987)
  AUTHORS   Klesse,L.J. and Parada,L.F.
  TITLE     p21 ras and phosphatidylinositol-3 kinase are required for survival
            of wild-type and NF1 mutant sensory neurons
  JOURNAL   J Neurosci 18 (24), 10420-10428 (1998)
   PUBMED   9852579
REFERENCE   7  (bases 1 to 6987)
  AUTHORS   Chantry,D., Vojtek,A., Kashishian,A., Holtzman,D.A., Wood,C.,
            Gray,P.W., Cooper,J.A. and Hoekstra,M.F.
  TITLE     p110delta, a novel phosphatidylinositol 3-kinase catalytic subunit
            that associates with p85 and is expressed predominantly in
            leukocytes
  JOURNAL   J Biol Chem 272 (31), 19236-19241 (1997)
   PUBMED   9235916
REFERENCE   8  (bases 1 to 6987)
  AUTHORS   Pons,S., Asano,T., Glasheen,E., Miralpeix,M., Zhang,Y.,
            Fisher,T.L., Myers,M.G. Jr., Sun,X.J. and White,M.F.
  TITLE     The structure and function of p55PIK reveal a new regulatory
            subunit for phosphatidylinositol 3-kinase
  JOURNAL   Mol Cell Biol 15 (8), 4453-4465 (1995)
   PUBMED   7542745
REFERENCE   9  (bases 1 to 6987)
  AUTHORS   Drachman,J.G., Griffin,J.D. and Kaushansky,K.
  TITLE     The c-Mpl ligand (thrombopoietin) stimulates tyrosine
            phosphorylation of Jak2, Shc, and c-Mpl
  JOURNAL   J Biol Chem 270 (10), 4979-4982 (1995)
   PUBMED   7534285
REFERENCE   10 (bases 1 to 6987)
  AUTHORS   Klippel,A., Escobedo,J.A., Hirano,M. and Williams,L.T.
  TITLE     The interaction of small domains between the subunits of
            phosphatidylinositol 3-kinase determines enzyme activity
  JOURNAL   Mol Cell Biol 14 (4), 2675-2685 (1994)
   PUBMED   8139567
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC110034.8 and AC130281.13.
            
            On or before Apr 7, 2020 this sequence version replaced
            XM_006535409.4, NM_008839.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219382.113322.1,
                                           SRR11927938.3329317.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-428               AC110034.8         57484-57911         c
            429-856             AC110034.8         18419-18846         c
            857-1066            AC110034.8         17649-17858         c
            1067-1317           AC110034.8         16909-17159         c
            1318-1563           AC110034.8         15027-15272         c
            1564-1649           AC110034.8         14264-14349         c
            1650-1755           AC110034.8         12145-12250         c
            1756-1908           AC110034.8         11245-11397         c
            1909-2043           AC110034.8         11027-11161         c
            2044-2168           AC110034.8         6897-7021           c
            2169-2250           AC110034.8         5372-5453           c
            2251-2415           AC110034.8         4903-5067           c
            2416-2519           AC110034.8         4611-4714           c
            2520-2691           AC110034.8         3049-3220           c
            2692-2798           AC110034.8         1009-1115           c
            2799-2920           AC110034.8         476-597             c
            2921-2999           AC130281.13        163118-163196       c
            3000-3170           AC130281.13        159257-159427       c
            3171-3288           AC130281.13        157692-157809       c
            3289-3440           AC130281.13        157441-157592       c
            3441-6987           AC130281.13        153182-156728       c
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 15.7 cM"
     gene            1..6987
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /note="phosphatidylinositol-4,5-bisphosphate 3-kinase
                     catalytic subunit alpha"
                     /db_xref="GeneID:18706"
                     /db_xref="MGI:MGI:1206581"
     exon            1..428
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            429..856
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             505..3711
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /EC_number="2.7.1.137"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.1.153"
                     /note="PI3K-alpha; PI3-kinase subunit alpha;
                     ptdIns-3-kinase subunit alpha; ptdIns-3-kinase subunit
                     p110-alpha; PI3Kalpha; serine/threonine protein kinase
                     PIK3CA; phosphoinositide-3-kinase catalytic alpha
                     polypeptide; phosphatidylinositol 3-kinase, catalytic,
                     alpha polypeptide; phosphatidylinositol 4,5-bisphosphate
                     3-kinase 110 kDa catalytic subunit alpha"
                     /codon_start=1
                     /product="phosphatidylinositol 4,5-bisphosphate 3-kinase
                     catalytic subunit alpha isoform"
                     /protein_id="NP_032865.2"
                     /db_xref="CCDS:CCDS38409.1"
                     /db_xref="GeneID:18706"
                     /db_xref="MGI:MGI:1206581"
                     /translation="MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLVT
                     IKHELFREARKYPLHQLLQDETSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVI
                     EPVGNREEKILNREIGFVIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPH
                     SRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPE
                     QVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSC
                     IMLGRMPNLMLMAKESLYSQLPIDSFTMPSYSRRISTATPYMNGETSTKSLWVINSAL
                     RIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIY
                     IPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPH
                     GLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSY
                     SHTGLSNRLARDNELRENDKEQLRALCTRDPLSEITEQEKDFLWSHRHYCVTIPEILP
                     KLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRSFAVRCLEKYLT
                     DDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQ
                     RFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMR
                     QPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNE
                     IIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHT
                     IMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIG
                     DRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQEYT
                     KTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDK
                     TEQEALEYFTKQMNDAHHGGWTTKMDWIFHTIKQHALN"
     exon            857..1066
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1317
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1563
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1649
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1755
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1908
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1909..2043
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2168
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2169..2250
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2251..2415
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2416..2519
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2520..2691
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2692..2798
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2799..2920
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2921..2999
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            3000..3170
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            3171..3288
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            3289..3440
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            3441..6987
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      6926
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
     regulatory      6968..6973
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /note="hexamer: CATAAA"
     polyA_site      6976
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
                     /note="major polyA site"
     polyA_site      6987
                     /gene="Pik3ca"
                     /gene_synonym="6330412C24Rik; caPI3K; p110; p110alpha"
ORIGIN      
        1 ctcctcagct ctcaccctcc tctgccccag gaggggggag ccgagggggt ggggaagagt
       61 tcgttgtttg tttacacgat gtgagcggaa agagagacca ataaagttta ttctggaaac
      121 aaaaggaaaa agagcccggg cgccggcgag cggagccggg ggtgggggcg tgcggcgggg
      181 ccggcgccga gcggagagag cgttcgagcg agcggcagtt ccggtgccgc ggcggccgag
      241 gctggggctg gggcggccgg cgaggcagcg ctcgggcccg gccgggccgc tccgcagcgg
      301 cgggggagag ggcccgggag gcgggcgccg cgccgcccgc tgtcctctcc ctcggcgccg
      361 ccgccgccgc cgcccgcggg gccgggaccc gatgcggcgg gagccgcgca gcctagagaa
      421 gccggagcgg cactgtttgg agacagtcgt gattctgact ccataaggcg gttttctatg
      481 taaagtttgc agagggtcag agcaatgcct ccacgaccat cttcgggtga actgtggggc
      541 atccacttga tgcccccacg aatcctagtg gaatgtttac tccccaatgg aatgatagtg
      601 actttagaat gcctccgtga ggccacactc gtcaccatca aacatgaact gttcagagag
      661 gccaggaaat accctctcca tcagcttctg caagacgaaa cttcttacat tttcgtaagt
      721 gtcacccaag aagcagaaag ggaagaattt tttgatgaaa caagacgact ttgtgacctt
      781 cggctttttc aacccttttt aaaagttatt gaaccagtag gcaaccgtga agaaaagatc
      841 ctcaatcgag aaattggttt tgttattggc atgccagtgt gtgaatttga tatggttaaa
      901 gatccagaag tccaagactt tcgaaggaac attctgaatg tttgcaaaga agctgtggac
      961 ctgcgggatc tcaactcgcc tcatagcaga gcaatgtatg tctaccctcc aaatgtcgag
     1021 tcttccccag aactgccaaa gcacatctac aacaagttag ataaaggaca aatcatagtg
     1081 gtgatttggg taatagtctc tccaaacaac gacaagcaga agtacactct gaagatcaat
     1141 catgactgtg tgccagagca agtcattgct gaagccatca ggaaaaagac tcggagcatg
     1201 ttgttgtcct ctgagcagct gaaactctgt gtcttagaat atcagggcaa gtatattctg
     1261 aaagtgtgtg gctgtgacga atacttcctg gaaaagtacc ctctgagtca gtacaagtac
     1321 ataagaagct gtataatgct ggggaggatg cccaacttga tgctgatggc caaagaaagc
     1381 ctatactctc agctgccgat tgatagcttc accatgccgt catactccag gcgcatctcc
     1441 acagccacac cctacatgaa tggagagaca tctacgaaat ccctctgggt cataaatagt
     1501 gcgctcagaa taaaaattct ttgtgcaacc tatgtaaatg taaatattcg agacattgat
     1561 aagatctatg ttcgaacagg tatctaccat ggaggagaac ccttatgtga caatgtgaac
     1621 actcaaagag taccttgttc caatcctagg tggaatgaat ggctgaatta tgatatatac
     1681 attcctgatc ttcctcgtgc tgcgcgcctt tgcctttcaa tctgctctgt taaaggccga
     1741 aagggtgcta aggaggagca ctgtccgttg gcctggggaa acataaactt gtttgattat
     1801 acagacaccc tagtgtccgg gaaaatggct ttgaatctct ggcctgtacc gcatgggtta
     1861 gaagatctgc tgaaccctat tggtgttact gggtcaaatc caaataaaga aactccatgc
     1921 ttagagttgg agtttgattg gttcagcagt gtggtgaagt ttccagacat gtctgtgatc
     1981 gaagaacatg ccaattggtc cgtgtcccga gaagctggat tcagttactc ccatacagga
     2041 ctgagtaaca gactagccag agacaatgag ttaagagaaa atgacaagga acagctccga
     2101 gcactttgca cccgggaccc actatctgaa atcactgaac aagagaaaga cttcctatgg
     2161 agccacagac actactgcgt aactattcct gaaatcctac ccaaattgct tctgtctgtc
     2221 aagtggaatt ccagagacga agtggcccag atgtactgct tagtaaaaga ttggcctcca
     2281 atcaaaccag agcaagccat ggaactcctg gactgtaact atccagatcc tatggttcgg
     2341 agttttgctg ttcggtgctt agaaaaatat ttaacagatg acaaactttc tcagtacctc
     2401 attcaacttg tacaggtctt aaaatatgaa cagtatttgg ataacctgct tgtgagattt
     2461 ttactcaaga aagcattgac aaatcaaagg attggccatt ttttcttttg gcatttaaaa
     2521 tctgagatgc acaataagac tgtcagtcag aggtttggcc tgctattgga gtcctactgc
     2581 cgtgcctgtg ggatgtatct gaagcacctg aacagacaag tagaggccat ggagaagctc
     2641 atcaacctaa cggacatcct taagcaggag aagaaggatg agacacaaaa ggtacagatg
     2701 aagtttttgg ttgaacagat gagacagcca gacttcatgg atgctttgca gggttttctg
     2761 tcccctctga atcctgctca ccaactagga aacctcaggc ttgaagagtg tcgaattatg
     2821 tcctctgcaa aaaggccact gtggttgaat tgggagaacc cagacatcat gtcagagcta
     2881 ctgtttcaga acaatgagat catctttaaa aatggcgacg acttacggca agatatgtta
     2941 acccttcaga tcatccgaat catggagaac atctggcaaa accaaggcct tgaccttcgc
     3001 atgctacctt atggctgtct atccattggg gactgtgtgg gtctcatcga ggtggtgaga
     3061 aactctcaca ccatcatgca aatccagtgc aaaggaggcc tgaagggggc gctgcagttc
     3121 aacagccaca cactgcatca atggctcaag gacaagaaca agggcgagat atatgatgca
     3181 gccattgacc tgttcactcg gtcctgcgct gggtactgcg tggcaacctt tatcttggga
     3241 attggagacc ggcacaacag caacatcatg gtgaaagatg acggacagct gtttcatata
     3301 gattttgggc actttttgga tcacaagaag aaaaaatttg gctataagcg ggaacgtgtg
     3361 ccatttgtgt tgacacagga tttcttgatt gtgattagta agggagcaca agagtacacc
     3421 aagaccagag agtttgagag gtttcaggag atgtgttaca aggcttacct agcaattcgg
     3481 cagcatgcca atctcttcat caaccttttt tcaatgatgc ttggctctgg aatgccagaa
     3541 ctacaatctt ttgatgacat tgcatatatc cgaaagactc tagccttgga caaaactgag
     3601 caagaagctt tggaatattt cacaaagcaa atgaatgatg cacatcatgg tggatggacg
     3661 acaaaaatgg attggatctt ccacaccatc aagcagcatg ctttgaactg agatgggagc
     3721 tgggactgcg agctggctcc cggcttctcc actgcatggc agtgagtggc agcaggcagg
     3781 cagtggcatg ggatggcaca gtcaggaaca acattaggac ttgagcaaga acataaacaa
     3841 cgtgctctat aattgaaaca ctgtacacgc aagcagggtt tgatagcact aaactagttt
     3901 atttcaaaat ccagctttag aataacgagc aatttcatgt tatgccttaa gtccaaaaaa
     3961 ggtaaacttt gaagattgtt tgtatctttt tttaaaaaaa caaaacaaaa caaaaatccc
     4021 caaaatacat acaaatgatg gagacaaaac tagaatgctg ttgtttgtct gccagtgttc
     4081 tgtctaacac gtggacacgc ccaaggctgt gactgctata ggtctgagta agctaaagct
     4141 tatattaagt tacatgaatt ggagaagaag gaaaattctg attttcccat tgctgttcag
     4201 acttaacgat ttgaagtggg gattttgatc cctgcttaat gaggaacaac acttggggtg
     4261 aagggactcg ctttttgctt tgaacaaaca acactttgat ggacttgggg tctcacctgc
     4321 ggttttgaaa gcagtcacaa tacttgcaga cagctgcgtt tttgttgggt gttgtttttt
     4381 gttgtttttt tctctggaca gtatttacaa ggatctgact tatttcctag ggaaattctg
     4441 ggctggcatc aagtatagca gtaagggcag aggagaggcc gcagggagcc cctgttcctg
     4501 acctgtacag tgttcacttt cagctgattg tttctccttt tgcaattgaa ctgaatactt
     4561 ttttttcatg catgttttcc agaaaataga agtgagtatt aatgttatta aaaagattat
     4621 ttttttttat taaaggctat ttatattata gaaactatca ttaatatata ttctttattt
     4681 acataatctg tcccatagtc atgcattgtt ttgcacccca aattttttat cgttggtaac
     4741 agcatggtta gcttttctct cggtctgtag atgaggctca ggcactattc catttatcca
     4801 atacccgtgt atgactcctt aaggaacaga ttgaatcgca cacgctttcc tgccgctgct
     4861 ggccgttttt tacacgacgc cctcatccat tcctccgtag ttgagacagc acaatcacat
     4921 gaaacccgtt actaaaatga atacaaaacc gactatcatc ctgactctta atccttctgc
     4981 agaggtctag tcatgagtga ctctttacat tatgagagac tggaaaccat gaatttttta
     5041 ccttcgtaag ctacgtatcc gtacttcaat gataaaagta ggacattaac ccattttact
     5101 atcatgtccc aattccaagt ggtgaggtct cactccagtg tcattaaatc ccattcaggc
     5161 acggtgcatt agtgagcatt ctgaaatgca tccatctaag atgtagggct aatgttagtg
     5221 gtcgctctag atttctactc agtgtttgaa tgattcatgt cctagaaaat agctttagca
     5281 gatactcaga tgccacacca aaaaaagaag aaaaaaagaa aaaaaaaaga ataaataaaa
     5341 aggaaaaaaa aaagaaaaac tgtgcaataa tctcctgaca gttcctagct caggcgttcc
     5401 tggggagcat cgttatgaag agcgcacgct gtacacgcta gaaaacagaa tccctgtaat
     5461 acagtgctca ccttgaggcc agacacctat atagacgttt tgaatagtga acaggggact
     5521 ctaatagaaa tactcttaat atctgcctat ttttagaacc cttaaagggc ataattattg
     5581 aagatttagg tacttcacta aagcatgtat atattattgc caacaagaaa aataaacctg
     5641 aagattaggg gaacttggtt ctgtaaactg tcttggaata gttaagcaga atttaagctc
     5701 tgttttatgc agaaaaccag atagattctt ttgcagatat agaaaacttc ctaacttatt
     5761 taaacttggc atttaacact ttgtgttact tagatataca gttgctaggt actaacatcc
     5821 cattcttctc tatatcaggg attattacag tcaaactcag tgacatggta caaatctaca
     5881 actttgatgg tgggaactga agaattacag agaactgtgt tttcccgagt gccaaaagaa
     5941 acaaaaacaa aacaaaacaa acaaacccca caaaaaaaag aaaaaaaaaa aaaagccgcg
     6001 agcctccttg cacaaaattg ataggttttt ttttgtgtgt atgtgtgtgt ttgtgtgtgt
     6061 gtatgtttaa cattagtcca tcagttgccg tagtatcacc tcccaggtct ctgcacaatt
     6121 aaaacacagc cacatagctt attttgtcat ttacaaccac attgatttta tttgaaagaa
     6181 agtctatagt ctgtgaaggg tataagtaaa gaaaggagaa ctggttgtat tcaaaaaggc
     6241 aaaagccaaa cttgaattgc agctggggtg atcaatatct gcttccagag agcaataatg
     6301 tgccacttac tcccaggtgc ttcagtacca gaaagtgctg tgggactcgg tcacctctgt
     6361 agatgaccgt ccttgtcagt gaatgactat tgtggaaatg acttgggctc catagtttca
     6421 ctaagtcact tgaggatgtc tcatcagcaa ttatttcagg aaggaaagaa agcaaatcta
     6481 aagaaagaaa ctaaatacac tgtagcaaga aataactctt caatcatgct ttaacttttt
     6541 accatagtct cagctataca aaaaacttta gtttgaagat tttacattgc tgttaatttg
     6601 aaatctgttg ttcttactgt ggagtttgat ttgttcgttt gtttgctttc tgttggtttt
     6661 tttttttttt tttaagatgt ttctaaatag attttttaaa aaaaagaaag aagaagaaga
     6721 atggaatctg gttgctattt taaggtagaa cctgagactt tttgtggttc ttcatgtcct
     6781 ctgtaaaatt tggtgtcaag agtcatcaac tctgaggttg tcccttctgt tctgctttat
     6841 attactgccc atcaggaaat gggaacctgg tgaatatata atgaattgta aaatatttta
     6901 aatgtgtaac tttttcaact gtgaaactga ctattggttt tttttttttt tgatgaaaac
     6961 agctgctcat aaagtatttt gtgtaaa
//
