LOCUS       NM_181414               3155 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus phosphatidylinositol 3-kinase catalytic subunit type 3
            (Pik3c3), transcript variant 1, mRNA.
ACCESSION   NM_181414
VERSION     NM_181414.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Courreges,C.J.F., Davenport,E.C.M., Bilanges,B., Rebollo-Gomez,E.,
            Hukelmann,J., Schoenfelder,P., Edgar,J.R., Sansom,D.,
            Scudamore,C.L., Roychoudhuri,R., Garden,O.A., Vanhaesebroeck,B. and
            Okkenhaug,K.
  TITLE     Lack of phosphatidylinositol 3-kinase VPS34 in regulatory T cells
            leads to a fatal lymphoproliferative disorder without affecting
            their development
  JOURNAL   Front Immunol 15, 1374621 (2024)
   PUBMED   39664390
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Feng,P., Yang,Q., Luo,L., Guan,Z., Fu,J., Zhao,M., Meng,W., Wan,S.,
            He,J., Li,Z., Wang,G., Sun,G., Dong,Z. and Yang,M.
  TITLE     Vps34 sustains Treg cell survival and function via regulating
            intracellular redox homeostasis
  JOURNAL   Cell Death Differ 31 (11), 1519-1533 (2024)
   PUBMED   39117783
  REMARK    GeneRIF: Vps34 sustains Treg cell survival and function via
            regulating intracellular redox homeostasis.
REFERENCE   3  (bases 1 to 3155)
  AUTHORS   Liu,T., Yuan,J., Dai,C., Chen,M.X., Fan,J., Humphreys,B.D.,
            Fulton,D.J.R., Kleven,D.T., Fan,X., Dong,Z. and Chen,J.K.
  TITLE     Pik3c3 expression profiling in the mouse kidney and its role in
            proximal tubule cell physiology
  JOURNAL   Am J Physiol Cell Physiol 327 (4), C1094-C1110 (2024)
   PUBMED   39250817
  REMARK    GeneRIF: Pik3c3 expression profiling in the mouse kidney and its
            role in proximal tubule cell physiology.
REFERENCE   4  (bases 1 to 3155)
  AUTHORS   Chen,S., Li,Z., Feng,J., Quan,Y., He,J., Hao,J. and Dong,Z.
  TITLE     Dual Activity of Type III PI3K Kinase Vps34 is Critical for NK Cell
            Development and Senescence
  JOURNAL   Adv Sci (Weinh) 11 (21), e2309315 (2024)
   PUBMED   38544346
  REMARK    GeneRIF: Dual Activity of Type III PI3K Kinase Vps34 is Critical
            for NK Cell Development and Senescence.
REFERENCE   5  (bases 1 to 3155)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 3155)
  AUTHORS   Lee,A.W. and States,D.J.
  TITLE     Colony-stimulating factor-1 requires PI3-kinase-mediated metabolism
            for proliferation and survival in myeloid cells
  JOURNAL   Cell Death Differ 13 (11), 1900-1914 (2006)
   PUBMED   16514418
REFERENCE   7  (bases 1 to 3155)
  AUTHORS   Lindmo,K. and Stenmark,H.
  TITLE     Regulation of membrane traffic by phosphoinositide 3-kinases
  JOURNAL   J Cell Sci 119 (Pt 4), 605-614 (2006)
   PUBMED   16467569
  REMARK    Review article
REFERENCE   8  (bases 1 to 3155)
  AUTHORS   Tuhackova,Z., Sloncova,E., Vojtechova,M. and Sovova,V.
  TITLE     IL2-dependent phosphorylation of 40S ribosomal protein S6 is
            controlled by PI-3K/mTOR signalling in CTLL2 cells
  JOURNAL   Int J Mol Med 13 (4), 601-605 (2004)
   PUBMED   15010863
REFERENCE   9  (bases 1 to 3155)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 3155)
  AUTHORS   Virbasius,J.V., Guilherme,A. and Czech,M.P.
  TITLE     Mouse p170 is a novel phosphatidylinositol 3-kinase containing a C2
            domain
  JOURNAL   J Biol Chem 271 (23), 13304-13307 (1996)
   PUBMED   8663140
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BE950444.1, AK044997.1, AC163399.6 and BE980562.1.
            
            On Nov 4, 2017 this sequence version replaced NM_181414.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057678.1, AK044997.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                BE950444.1         6-37
            33-1620             AK044997.1         2-1589
            1621-1621           AC163399.6         118742-118742       c
            1622-3131           AK044997.1         1590-3099
            3132-3155           BE980562.1         1-24                c
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 17.41 cM"
     gene            1..3155
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /note="phosphatidylinositol 3-kinase catalytic subunit
                     type 3"
                     /db_xref="GeneID:225326"
                     /db_xref="MGI:MGI:2445019"
     exon            1..154
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..2750
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /EC_number="2.7.1.137"
                     /note="PI3K type 3; PI3-kinase type 3; ptdIns-3-kinase
                     type 3; phosphoinositide-3-kinase, class 3"
                     /codon_start=1
                     /product="phosphatidylinositol 3-kinase catalytic subunit
                     type 3"
                     /protein_id="NP_852079.2"
                     /db_xref="CCDS:CCDS37752.1"
                     /db_xref="GeneID:225326"
                     /db_xref="MGI:MGI:2445019"
                     /translation="MGEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPML
                     KFSGLYQETCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPR
                     NAQVALTIWDVYGPGSAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSEPTRTP
                     GRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLM
                     VEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARS
                     LRSGPSDHDLKPNATTRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFL
                     KCVNWDLPQEAKQALELLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDL
                     LMYLLQLVQALKYENFDDIKNGLEPTKKDSQTSASESLSNSGVSSGDIDSSQIITNPL
                     PPVASPPPASKAKEVSDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQ
                     RDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAVQRESGNR
                     KKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETATLFKSALMPAQLFFK
                     TEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFM
                     QFIQSVPVAEVLDTEGSIQNFFRKYAPSETGPYGISAEVMDTYVKSCAGYCVITYILG
                     VGDRHLDNLLLTKTGKLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEF
                     RKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAV
                     HYMQSLIDESVHALFAAVVEQIHKFAQYWRK"
     exon            155..343
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            344..487
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            488..617
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            618..704
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            705..800
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            801..872
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            873..977
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1070
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1256
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1411
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..1502
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            1503..1570
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1676
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1793
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1925
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..2054
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2124
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            2125..2189
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2274
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            2275..2349
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            2350..2518
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            2519..2609
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            2610..2735
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     exon            2736..3139
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3112..3117
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /note="hexamer: AGTAAA"
     polyA_site      3133
                     /gene="Pik3c3"
                     /gene_synonym="5330434F23Rik; Vps34"
                     /note="major polyA site"
ORIGIN      
        1 ttttcatgtt ttgatgcagc ggcggtttct tcctggagtc ttcctcctcg cgggctgtct
       61 ctgtggcgtc tgggctccga gctgtcatgg gggaggcgga gaagttccac tacatctaca
      121 gctgtgacct ggacatcaac gtgcagctga agatagggag cttggaaggg aagagagaac
      181 aaaagagcta taaagctgtc ctagaagatc ccatgttaaa gttttctggg ctataccaag
      241 agacatgctc cgacctctat gtgacttgtc aagtgtttgc tgaagggaag cccctggcct
      301 taccagtcag aacatcctac aaggcgttta gtacaagatg gaattggaac gagtggctga
      361 aacttccggt gaagtaccct gacctgccca ggaatgccca agtggccttg actatatggg
      421 atgtgtatgg accgggaagc gctgtgccag tgggaggaac aaccgtgtcg ctctttggaa
      481 aatatggcat gtttcgccaa ggaatgcatg acttgaaggt ctggcctaac gtggaggcag
      541 atggttctga acctacaaga actcccggca gaacaagcag cacactgtca gaagatcaga
      601 tgagccgcct cgccaagctc accaaggctc atcggcaagg acacatggtg aaagtggatt
      661 ggctggacag attaacattt agagagatag aaatgataaa tgagagtgag aaacgaagct
      721 ctaatttcat gtacttgatg gttgagtttc gctgtgtcaa gtgcgatgac aaggagtatg
      781 gcattgttta ctatgaaaag gatggtgacg aatcatctcc aattttaacc agttttgagt
      841 tagtgaaagt tcctgatcct caaatgtcta tggagaattt agtggagagc aaacaccaca
      901 agcttgctcg gagcttaaga agcgggccat ctgaccacga tctcaaaccc aacgctacca
      961 caagagatca gctaaatatt attgtgagtt acccaccaac caagcaactc acatatgaag
     1021 aacaagatct tgtttggaaa tttagatatt atcttactaa tcaagaaaaa gctctgacga
     1081 agttcttgaa gtgtgttaat tgggacctgc cccaggaggc caagcaggcc ctggaacttc
     1141 tgggaaagtg gaagccaatg gatgtagagg actccctgga gctgctctcc tctcattaca
     1201 ccaaccccac cgtgaggcgc tacgctgtgg cccgcctgag gcaggccgat gatgaggatt
     1261 tgctgatgta cttactgcag ctggtccagg ctctgaaata tgaaaacttt gatgacataa
     1321 agaatggttt ggaacctacc aagaaagata gtcaaacttc agcatcagaa agtctgtcaa
     1381 attctggagt cagttctgga gacatagata gctcccaaat tataaccaac cctcttcctc
     1441 ccgtggcctc ccctcctcct gcatctaaag caaaggaagt ttccgatggg gaaaatctcg
     1501 agcaagatct atgtacgttc ttgatatcaa gagcctgtaa gaactcaaca ctggctaatt
     1561 atttatactg gtatgtgata gtggagtgtg aagatcagga cacccagcag cgggacccaa
     1621 agactcacga gatgtacctg aacgtgatga ggcgcttcag ccaagccttg ctcaagggtg
     1681 acaagtctgt gagagtcatg cgctccctgc tggcggctca gcagaccttt gtagatcgcc
     1741 tggtccatct gatgaaggcg gtgcagagag aaagtgggaa ccgcaagaag aagaatgaga
     1801 gacttcaggc cttgcttgga gataatgaga aaatgaactt atcagatgtg gaactgatcc
     1861 cattgccgct ggagccacag gtgaaaataa ggggcatcat cccggaaaca gctaccctgt
     1921 tcaagagtgc tcttatgcct gcccagctgt tcttcaagac tgaagatgga ggcaaatacc
     1981 cagttatttt caagcatgga gacgacttgc gtcaagatca gcttattctt cagatcatct
     2041 ccctcatgga caagctgtta cggaaagaaa acctggattt gaaattgacc ccatacaagg
     2101 tgttagccac tagcacaaaa catggcttca tgcagttcat ccagtcggtt cctgtcgcag
     2161 aagttcttga cacagaggga agcattcaga actttttcag gaaatatgcc cccagtgaga
     2221 ctggacccta tggcatcagt gcagaggtca tggacactta cgttaaaagc tgtgctggat
     2281 actgtgtgat tacgtacatt cttggagttg gagaccggca cctggataac cttttgttaa
     2341 caaagacagg caaactcttc catatagatt tcgggtatat tttgggtcga gatccgaagc
     2401 ccctccctcc tccgatgaag ctgaacaagg agatggtgga agggatgggt ggcacccaga
     2461 gtgagcagta ccaagagttc cgaaagcagt gctacacagc cttcctccac ctgcgaaggt
     2521 attccaatct gatcttgaac ttgttctcct tgatggttga tgcaaacatt ccagatattg
     2581 ctcttgaacc agataaaact gtaaaaaagg ttcaggataa gttccgcctg gacctgtcag
     2641 atgaggaggc tgtgcactac atgcagagtc tgatcgacga gagcgtccac gcactgttcg
     2701 ccgccgtggt ggagcagatc cataagtttg cccagtactg gagaaaatga aaggggattt
     2761 gacccatcct gatgcctggc tcaataagaa aacacaggag aagccactgc tgtgtactaa
     2821 agacatcaaa gaaagaacaa ggaagagagg gtgtccagaa gttacgtcgt gcctgggctt
     2881 tgtgtccttg catgccactg cttaaatcca gcccacaggg tttgtttaaa gctgctgcag
     2941 acagagtagt cgctcatctc taattccaaa actctggagg ccgaagtgtg aagagtgaca
     3001 catttttgaa gccaccctca gctacataat gacttctggg tcagccaaaa atcactgtat
     3061 atatttgtgt tatagtgtat aaagatggaa agaggattat gtatatgaaa cagtaaagca
     3121 ttttaccctc tgagtctcaa aaaaaaaaaa aaaaa
//
