LOCUS       NM_024464               1019 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            X (Pigx), transcript variant 1, mRNA.
ACCESSION   NM_024464 XM_991886
VERSION     NM_024464.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1019)
  AUTHORS   Rutkowski,T.P., Purcell,R.H., Pollak,R.M., Grewenow,S.M.,
            Gafford,G.M., Malone,T., Khan,U.A., Schroeder,J.P., Epstein,M.P.,
            Bassell,G.J., Warren,S.T., Weinshenker,D., Caspary,T. and
            Mulle,J.G.
  TITLE     Behavioral changes and growth deficits in a CRISPR engineered mouse
            model of the schizophrenia-associated 3q29 deletion
  JOURNAL   Mol Psychiatry 26 (3), 772-783 (2021)
   PUBMED   30976085
REFERENCE   4  (bases 1 to 1019)
  AUTHORS   Lukacs,M., Roberts,T., Chatuverdi,P. and Stottmann,R.W.
  TITLE     Glycosylphosphatidylinositol biosynthesis and remodeling are
            required for neural tube closure, heart development, and cranial
            neural crest cell survival
  JOURNAL   Elife 8, e45248 (2019)
   PUBMED   31232685
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1019)
  AUTHORS   Zhu,Y., Zhuo,J., Li,C., Wang,Q., Liu,X. and Ye,L.
  TITLE     Regulatory network analysis of hypertension and hypotension
            microarray data from mouse model
  JOURNAL   Clin Exp Hypertens 40 (7), 631-636 (2018)
   PUBMED   29400567
  REMARK    GeneRIF: Sept6 and Pigx might participate in the pathogenesis of
            high blood pressure.
REFERENCE   6  (bases 1 to 1019)
  AUTHORS   Ashida,H., Hong,Y., Murakami,Y., Shishioh,N., Sugimoto,N.,
            Kim,Y.U., Maeda,Y. and Kinoshita,T.
  TITLE     Mammalian PIG-X and yeast Pbn1p are the essential components of
            glycosylphosphatidylinositol-mannosyltransferase I
  JOURNAL   Mol Biol Cell 16 (3), 1439-1448 (2005)
   PUBMED   15635094
  REMARK    GeneRIF: A non-AUG translation initiation site (CUG) is used in rat
            Pigx
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY708475.1, AK008583.2 and
            CF581933.1.
            
            On Nov 30, 2007 this sequence version replaced NM_024464.2.
            
            Summary: This gene encodes a type I transmembrane protein in the
            endoplasmic reticulum (ER). The protein is an essential component
            of glycosylphosphatidylinositol-mannosyltransferase I, which
            transfers the first of the four mannoses in the GPI-anchor
            precursors during GPI-anchor biosynthesis. Studies in rat indicate
            that the protein is translated from a non-AUG translation
            initiation site. Multiple transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK008583.2, CA978632.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164137
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: PMID: 15635094
            RefSeq Select criteria   :: based on conservation, expression,
                                        longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-118               BY708475.1         1-118
            119-982             AK008583.2         121-984
            983-1019            CF581933.1         2-38                c
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 22.4 cM"
     gene            1..1019
                     /gene="Pigx"
                     /gene_synonym="2010319C14Rik; PIG-X"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class X"
                     /db_xref="GeneID:72084"
                     /db_xref="MGI:MGI:1919334"
     exon            1..258
                     /gene="Pigx"
                     /gene_synonym="2010319C14Rik; PIG-X"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..923
                     /gene="Pigx"
                     /gene_synonym="2010319C14Rik; PIG-X"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; non-AUG (CUG) translation initiation codon;
                     phosphatidylinositol glycan, class X;
                     phosphatidylinositol-glycan biosynthesis class X protein"
                     /codon_start=1
                     /product="phosphatidylinositol-glycan biosynthesis class X
                     protein isoform 1 precursor"
                     /protein_id="NP_077784.2"
                     /db_xref="CCDS:CCDS28113.2"
                     /db_xref="GeneID:72084"
                     /db_xref="MGI:MGI:1919334"
                     /translation="MAAGAVAWLLLWAAWLVGRLAADFSDAPFSAGKGVRATCSEIIL
                     RQEFLKDGFHRDLLIKVKFGESIEDLQTCRLLIKHYIPPGLFVDPYELASLRERNLTE
                     AVMLSESFNIEAPNYLSNESAVLIYARQDAQCIDCFQAFLPVHYRYHRPHKKDGDTLI
                     VVNNPDLLMYCDQEFPILKCWAQSEVAAPCALKSEEICQWKSMQYKSILKNLTVQVPV
                     GLTIHTSLVCSVTLLITILCSTLILLAVFKYGHFSL"
     exon            259..322
                     /gene="Pigx"
                     /gene_synonym="2010319C14Rik; PIG-X"
                     /inference="alignment:Splign:2.1.0"
     exon            323..464
                     /gene="Pigx"
                     /gene_synonym="2010319C14Rik; PIG-X"
                     /inference="alignment:Splign:2.1.0"
     exon            465..678
                     /gene="Pigx"
                     /gene_synonym="2010319C14Rik; PIG-X"
                     /inference="alignment:Splign:2.1.0"
     exon            679..779
                     /gene="Pigx"
                     /gene_synonym="2010319C14Rik; PIG-X"
                     /inference="alignment:Splign:2.1.0"
     exon            780..990
                     /gene="Pigx"
                     /gene_synonym="2010319C14Rik; PIG-X"
                     /inference="alignment:Splign:2.1.0"
     regulatory      956..961
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigx"
                     /gene_synonym="2010319C14Rik; PIG-X"
                     /note="hexamer: AATAAA"
     polyA_site      990
                     /gene="Pigx"
                     /gene_synonym="2010319C14Rik; PIG-X"
                     /note="major polyA site"
ORIGIN      
        1 tccggaacct ggagttgtag ggagatccta gggtggaagt gttagccgag ttctgttcgg
       61 gtcatcggga aaggtgagcg cgcgctcagc gtgctcagat tccttcggtg accgcagctt
      121 gccccgagcg cccctttcgg gcgtcgggct ccggcgtcct ggctgccggc gctgtggcct
      181 ggctgctcct ttgggcagcc tggctggtcg gcaggctcgc cgcggacttc agcgacgccc
      241 ctttcagcgc tggtaaaggt gtaagggcca cttgttctga aattatttta aggcaagaat
      301 ttttgaaaga tggtttccac agagaccttt taataaaagt gaaatttgga gagagcattg
      361 aggacctaca gacctgccgg ctcctaatta agcattacat tccaccagga ctctttgtgg
      421 atccctatga gttggcttca ctacgagaaa ggaacttaac agaggcagtg atgctatcag
      481 aaagttttaa tatagaagcc cccaactact tgtccaatga atctgcagtc ctcatttatg
      541 cccggcagga tgcacagtgc atcgactgct tccaggcctt tttacctgtg cactatcgct
      601 atcaccggcc acataagaaa gatggagaca ccctcattgt ggtcaacaac cccgacttac
      661 tgatgtactg tgaccaagag tttccaattt tgaaatgctg ggctcagtca gaagtagcag
      721 ctccttgtgc tttgaagagt gaggagatct gccagtggaa aagcatgcag tacaaatcaa
      781 tacttaagaa tttgacagtg caggttccag tgggactgac tatacatacc tctttagtgt
      841 gttctgtgac gctgctcatt acgattctgt gttctacttt gatcctttta gctgttttca
      901 aatatggcca cttttccctg taagttttgt acagctaagt gttttctatg aacctaataa
      961 atgagacaag agtgctcttt tcagaactaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//
