LOCUS       NM_001077636            2258 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            W (Pigw), transcript variant 2, mRNA.
ACCESSION   NM_001077636
VERSION     NM_001077636.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Sagane,K., Umemura,M., Ogawa-Mitsuhashi,K., Tsukahara,K., Yoko-o,T.
            and Jigami,Y.
  TITLE     Analysis of membrane topology and identification of essential
            residues for the yeast endoplasmic reticulum inositol
            acyltransferase Gwt1p
  JOURNAL   J Biol Chem 286 (16), 14649-14658 (2011)
   PUBMED   21367863
  REMARK    GeneRIF: analysis of essential residues of PIG-W and Gwt1p and
            membrane topology of Gwt1p
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Lin,Q., Donahue,S.L., Moore-Jarrett,T., Cao,S., Osipovich,A.B. and
            Ruley,H.E.
  TITLE     Mutagenesis of diploid mammalian genes by gene entrapment
  JOURNAL   Nucleic Acids Res 34 (20), e139 (2006)
   PUBMED   17062627
REFERENCE   3  (bases 1 to 2258)
  AUTHORS   Murakami,Y., Siripanyapinyo,U., Hong,Y., Kang,J.Y., Ishihara,S.,
            Nakakuma,H., Maeda,Y. and Kinoshita,T.
  TITLE     PIG-W is critical for inositol acylation but not for flipping of
            glycosylphosphatidylinositol-anchor
  JOURNAL   Mol Biol Cell 14 (10), 4285-4295 (2003)
   PUBMED   14517336
REFERENCE   4  (bases 1 to 2258)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK086537.1 and CD547872.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK086537.1, AK011762.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                AK086537.1         2-59
            59-1728             AK086537.1         60-1729
            1729-2258           CD547872.1         1-530               c
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 51.34 cM"
     gene            1..2258
                     /gene="Pigw"
                     /gene_synonym="2610044A17Rik; Gwt1; PIG-W"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class W"
                     /db_xref="GeneID:70325"
                     /db_xref="MGI:MGI:1917575"
     exon            1..58
                     /gene="Pigw"
                     /gene_synonym="2610044A17Rik; Gwt1; PIG-W"
                     /inference="alignment:Splign:2.1.0"
     exon            59..2258
                     /gene="Pigw"
                     /gene_synonym="2610044A17Rik; Gwt1; PIG-W"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..1581
                     /gene="Pigw"
                     /gene_synonym="2610044A17Rik; Gwt1; PIG-W"
                     /note="phosphatidylinositol glycan, class W"
                     /codon_start=1
                     /product="phosphatidylinositol-glycan biosynthesis class W
                     protein"
                     /protein_id="NP_001071104.1"
                     /db_xref="CCDS:CCDS36263.1"
                     /db_xref="GeneID:70325"
                     /db_xref="MGI:MGI:1917575"
                     /translation="MSQKQLKEAFVRNLSGTSVLEVTQGLCFPAFCILCRGLWIIFSQ
                     HVCSFSNTWSTRFLMDFVVLIVPLVITLTVLSSFILLENLTVIVWGAWLLYQIYHRRT
                     CYAKVPVQKVFANFLKISLESEYNPAITCYRVINSVFTAIAILAVDFPLFPRRFAKTE
                     LYGTGAMDFGVGGFIFGAAMVCPEVRRKSIEESRFNYLRKSLYSVWPLVFLGMGRLVI
                     IKSIGYQEHSTEYGIHWNFFFTIIVVRLVTSLLLIIFPLNKSWIVAVSITVVYQLALD
                     YTPLKRILLYGTDGSGTRVGFLNANREGIISTLGYVTIHMAGVQTGLYVLKGRAQVRD
                     WIKATCWVFSVAVGFFISLHIVQVNIEAVSRRMANLAFCLWVVASSLMLLSCLLLSGI
                     ILSFAQFLIKGSLVPCSWKLIQSPTTHKNHSESLILEAEKNQPSLCLITALNRNQLFF
                     FLLSNITTGLINLTMDTLHTGALWTLVVLSIYMFTNCLVIYVLDLQGKTIKFW"
     regulatory      1712..1717
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigw"
                     /gene_synonym="2610044A17Rik; Gwt1; PIG-W"
                     /note="putative"
ORIGIN      
        1 gcgggacgca cgtggcccgt cccaggccgc cggctccggc tagcgggctg ttcgcgagga
       61 aggaagaaaa tgtctcaaaa gcagctgaag gaagcctttg tcaggaacct cagtgggacc
      121 agtgtgctgg aagtgaccca gggcctgtgc tttcctgcct tctgtatcct gtgtagaggc
      181 ctttggatca tcttctcaca gcacgtatgc tctttctcaa acacctggag cacacgattc
      241 ctcatggact ttgttgtctt gatagtcccc ctagtgatca ctctgactgt attgtcttcc
      301 tttatcctcc tcgagaatct cactgtgatt gtctgggggg catggctgtt gtatcagata
      361 taccacagga ggacttgcta cgccaaagtg cctgtccaga aagtctttgc aaacttcttg
      421 aaaatcagcc tagaatctga gtacaatccc gcaatcacct gttaccgggt cattaacagc
      481 gtatttacgg ctattgccat tttggccgtg gatttcccac ttttccctag aagatttgcc
      541 aaaactgaac tttatgggac aggagcaatg gattttggag taggtggctt tatttttggg
      601 gcggcaatgg tttgtccaga ggttagaagg aaatctatag aagagtctag atttaattat
      661 cttagaaaat cgctgtattc tgtctggcca ttggtcttct taggaatggg acggttggtc
      721 attataaagt ccataggcta ccaggaacat tctaccgagt acggcattca ctggaacttt
      781 ttctttacca tcatagttgt gcgattagta acgtcactgc ttttgattat ttttccttta
      841 aataagtcgt ggattgtggc tgtcagcata actgtggtat accagctagc cctggattat
      901 actccactca agaggatact tttgtatggc actgatggca gtggcacaag ggttggcttc
      961 ttaaatgcta accgagaggg aataatttcc actttggggt atgtgacaat acacatggct
     1021 ggtgtacaaa cgggattgta tgtgcttaag ggaagagcac aagtcagaga ctggataaaa
     1081 gctacttgtt gggttttctc agtagctgtt ggcttcttca tttctcttca tatagtgcaa
     1141 gtaaacatag aagcagtatc tcgaagaatg gccaatttag ccttttgtct gtgggtagtt
     1201 gcttccagtc tgatgcttct tagttgtctg ttactgagtg gtataatttt gagttttgcc
     1261 caatttctaa ttaaagggtc tctagttcca tgttcttgga aacttataca gtctcctact
     1321 acacataaaa accattctga atctctaatc ttagaagctg aaaaaaacca acccagtctt
     1381 tgtttgatca cagctctgaa cagaaaccag ctgttttttt tcttactgtc aaatataaca
     1441 actggtctga ttaacctgac gatggataca ttacacactg gtgccttgtg gaccttagtt
     1501 gtgctcagta tttatatgtt taccaactgt ttagttatat atgtacttga tttacaaggt
     1561 aaaaccataa agttttggtg atgagtgggt aagaaatatg tatttgaata acattatttt
     1621 agtggagaag aataagaagg gaggaaatgt gttcttagca atctagtctt aaccatactt
     1681 tattatgaag tttcagtctt ctatgacaga aattaaactt tacctttttt aaaggaagat
     1741 tcatggatgt ttaatacata tcaacattat gtaaaataag ccaaaagaag tggcacaaag
     1801 ctttaatccc agaacatggg aggcagaggc aggcggatct ctgtgagttg gaagccagcc
     1861 tggtctgcat gttgagtttc aggacaccca aggctatgta cagagacccg gtgggggggc
     1921 ggggaggggg gcaaagaata gaagtaaaaa tcttgttatt tattggaact tagaaaacac
     1981 cagtatttta aagaacttgt tcatagattt tgctttgcct ggagttcaca gttgtactct
     2041 tacctcaggc tcaatgctgg gatttcaggc gtgagccacc acacccactt gttgatagtt
     2101 ttgacactgg aaggtgaatg gcggtaattt ttactttcta tatatcatta tggcactgta
     2161 tttgtggttt ttatatgaat atgtaattgt gattataatg tttgtgttat atgtgtttac
     2221 attaagatct gaattattta aaatttttat gtgaatgc
//
