LOCUS       NM_178698               2941 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            V (Pigv), transcript variant 1, mRNA.
ACCESSION   NM_178698
VERSION     NM_178698.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Rodriguez de Los Santos,M., Rivalan,M., David,F.S., Stumpf,A.,
            Pitsch,J., Tsortouktzidis,D., Velasquez,L.M., Voigt,A.,
            Schilling,K., Mattei,D., Long,M., Vogt,G., Knaus,A.,
            Fischer-Zirnsak,B., Wittler,L., Timmermann,B., Robinson,P.N.,
            Horn,D., Mundlos,S., Kornak,U., Becker,A.J., Schmitz,D., Winter,Y.
            and Krawitz,P.M.
  TITLE     A CRISPR-Cas9-engineered mouse model for GPI-anchor deficiency
            mirrors human phenotypes and exhibits hippocampal synaptic
            dysfunctions
  JOURNAL   Proc Natl Acad Sci U S A 118 (2) (2021)
   PUBMED   33402532
  REMARK    GeneRIF: A CRISPR-Cas9-engineered mouse model for GPI-anchor
            deficiency mirrors human phenotypes and exhibits hippocampal
            synaptic dysfunctions.
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Li,Y., Klena,N.T., Gabriel,G.C., Liu,X., Kim,A.J., Lemke,K.,
            Chen,Y., Chatterjee,B., Devine,W., Damerla,R.R., Chang,C., Yagi,H.,
            San Agustin,J.T., Thahir,M., Anderton,S., Lawhead,C., Vescovi,A.,
            Pratt,H., Morgan,J., Haynes,L., Smith,C.L., Eppig,J.T.,
            Reinholdt,L., Francis,R., Leatherbury,L., Ganapathiraju,M.K.,
            Tobita,K., Pazour,G.J. and Lo,C.W.
  TITLE     Global genetic analysis in mice unveils central role for cilia in
            congenital heart disease
  JOURNAL   Nature 521 (7553), 520-524 (2015)
   PUBMED   25807483
REFERENCE   3  (bases 1 to 2941)
  AUTHORS   Kang,J.Y., Hong,Y., Ashida,H., Shishioh,N., Murakami,Y.,
            Morita,Y.S., Maeda,Y. and Kinoshita,T.
  TITLE     PIG-V involved in transferring the second mannose in
            glycosylphosphatidylinositol
  JOURNAL   J Biol Chem 280 (10), 9489-9497 (2005)
   PUBMED   15623507
REFERENCE   4  (bases 1 to 2941)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK080590.1, AK046527.1 and BX537327.7.
            
            On Mar 21, 2009 this sequence version replaced NM_178698.4.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). Variants 1 and 4 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK052446.1, AK080858.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                AK080590.1         2-42
            42-2111             AK046527.1         3-2072
            2112-2941           BX537327.7         45527-46356         c
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 66.25 cM"
     gene            1..2941
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class V"
                     /db_xref="GeneID:230801"
                     /db_xref="MGI:MGI:2442480"
     exon            1..329
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            330..452
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             375..1856
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /EC_number="2.3.1.225"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phosphatidylinositol glycan, class V; GPI
                     mannosyltransferase 2; PIG-V; GPI-MT-II; GPI
                     mannosyltransferase II; phosphatidylinositol-glycan
                     biosynthesis class V protein"
                     /codon_start=1
                     /product="GPI mannosyltransferase 2 isoform 1"
                     /protein_id="NP_848813.3"
                     /db_xref="CCDS:CCDS18755.1"
                     /db_xref="GeneID:230801"
                     /db_xref="MGI:MGI:2442480"
                     /translation="MGLLDPSQKEVLRFAVNCRILTLVLQALFNLIIPDHHADAFCPP
                     RLAPSGSADQLVEGLLGGLSRWDAEHFLFIAEHGYLYEHNFAFFPGFPLALLMGTELL
                     RPLQGLLSQRSCLLVSVALLNLLFSVLAAVALHDLGCLVLHCPRQALCAALLFCISPA
                     NVFLAAGYSEALFAFLTFSAMGQLERGRGWASGLLFALAAGVRSNGLVSLGFLLHSQC
                     RGFCSSLAVLSPWKPLVKLMASVCLSVLIVSLPFALFQYRAYIQFCSPGSAPSIPEPL
                     LQLAADKGYRLAGENAPPWCSWDLPLIYNYIQDVYWNVGLLRYYELKQVPNFLLATPV
                     TVLVVWATWTYVTTHPWLCLTLGLQRTKDRENPEKPHRGFLSPKVFVYLVHAAALLVF
                     GGLCMHVQVLTRFLASSTPIMYWFPAHLLQDQEPLLRCVDTEPGKLPQEKSPPGQKAP
                     RNCLMKLFYDWKRCSPVTRCVLVYFLTYWLLGLILHCNFLPWT"
     exon            453..1574
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1575..2941
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2115
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
     regulatory      2920..2925
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2941
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /note="major polyA site"
ORIGIN      
        1 gagccggcgc gcaggcgcgg cacggacggg acccggatgg aggcgctgaa gttggagctc
       61 cgcgggggtg gtctggacgc gcgggcgcgg aggatccggc gccagctctg caactctgga
      121 gtcctccggg gagggaagct gtcaaacgca ggccgagaga aattaggcgg aggctgtaga
      181 gaagcgagga gccccgcccc tccgccatag cagcctccgg cgccgcctct tcctttgcag
      241 cctccggccc tcgtctgctt ccggccccgt tgcctggccc ggccccgcga ttcccccggg
      301 acgaagccct gggccgtggc tcccttggga atttagaagc cggaggaagc tcagtcctgg
      361 tggtgagaga gaggatggga ctcctggacc catcccagaa agaggtgctg agattcgcag
      421 taaactgccg catcctgact ctagtgctac aggcactctt caacctcatc atcccagatc
      481 accacgcaga tgccttttgc cctccccgcc tcgccccctc gggctctgcg gatcagcttg
      541 tggaaggtct tctgggtggt ctgtctcgat gggatgctga acacttcctg tttatcgctg
      601 agcacggcta cctttatgag cacaactttg ccttcttccc tggctttccc ttggccctgc
      661 tgatggggac tgagctgctg agacccttgc agggcctctt gagccagcgc agttgcctgc
      721 tggtctcagt agcacttctc aacttgctgt tctccgtgct ggctgcagtg gcactccatg
      781 acttgggttg tctggttctg cactgtcctc ggcaggcctt gtgtgcagcg ttgctcttct
      841 gcatcagtcc tgccaacgtt ttcctggcag ccggctactc agaggctttg tttgccttcc
      901 tgacgttcag cgccatgggg cagctggaga ggggccgtgg ctgggctagt gggctgctct
      961 ttgctcttgc tgctggggtg cgctccaatg ggctggtgag ccttggcttc ctcctgcact
     1021 cccagtgcag aggcttttgt tcctctctgg cggtgctgag cccctggaag cccctcgtga
     1081 agctgatggc ctctgtgtgc ttgtcagtgc tcatagtcag ccttcccttc gccctctttc
     1141 agtatcgtgc ctacatccag ttctgctcac caggctcagc cccctccatc cctgagccct
     1201 tgctgcagct agctgccgac aaaggctacc ggcttgcagg agaaaatgca cccccatggt
     1261 gctcctggga ccttcctcta atatacaact atattcagga tgtctactgg aatgttggtc
     1321 ttctgcgata ctatgagctc aaacaggtgc ccaatttcct actagctaca ccagtgacag
     1381 tactggttgt gtgggcaacc tggacatatg tgaccaccca cccttggctc tgccttacac
     1441 ttgggctgca aagaaccaag gacagggaga acccggagaa gccccaccgt ggcttcctga
     1501 gtccgaaggt gtttgtgtac ctggtccacg ctgcagcact gctggtcttt ggaggtctgt
     1561 gcatgcatgt ccaggttctc acgcggtttt tggcctcttc cactcctatc atgtactggt
     1621 ttccagctca cctgcttcag gatcaagaac cgctgctgag gtgtgtggac acagagcctg
     1681 ggaagcttcc tcaggagaag tccccaccag gacagaaggc ccccagaaac tgtctcatga
     1741 aactcttcta cgattggaag agatgctctc cagtcacaag atgcgttctg gtctacttcc
     1801 tgacctactg gcttctggga ctaattctcc attgcaactt cctgccttgg acttgacctg
     1861 gagtgtcaag gaatgaaagc tctaagacga caccctggct gggacagccc gcacactccg
     1921 gagagcactg tgcagtagga tctctgggtc tctctggggg ccagctaaga agggaaaatg
     1981 ggagcacagg cgccccttct catcctgggc ggggcaggtg taggggagta acttcagtgc
     2041 tcagtgcagg ggtctcagat ctccctagtc cacaagtact aggcaatctt aaacctacta
     2101 ctgatccacg tgtaagtctg tcttcttgtt ttttgagaga aggttggttt ctctacattg
     2161 ccctggttgt cctggaactc actctgtaga ccaagtttat gtagaccagg ctggccttga
     2221 actcagagat tcatctgcct ctgcctcttg agtactaggc ccaccactga gtgtgagcca
     2281 cccctgcctg gcctggtgga taaatttaaa tgtttgatca aagcacaggc caacagcctg
     2341 ccccactcag gatggtggag tcagtgcagt gtagtgacag aaggaagagg ggacagacag
     2401 cctgctctaa gtgagaggtt tctgaaggtg gttgttttct tttttttttt ttttaaagat
     2461 gtattatatt atatatgagt acactagctg tcttcagaca caccagaaga gggcatcaga
     2521 tcccattgca gatggttgtg agccaccatg tggctgctgg gaattgaact caggacctct
     2581 ggaagagcag ccagtgctct taaccactga gccatctctc cagctccctg aagttttctt
     2641 tatgatactg gtgtgtgtgt gtctgtgtct atcctacaga ctgtgaaaat ctaaatactc
     2701 cttttgcaat gaattacctt tttctggagg aggggtagtc aggggagcta agacaaggtc
     2761 cctctgtgta actctgactg tcctagaact tgcttgctat gtagatctgg ctggttttga
     2821 actcagagat cctcctacct ctgccgaact agtttctata aattatagag ggttgaggct
     2881 agaactccgt agtaaagcac acgaggccct aatttcagta ataaacacca cttggatcag
     2941 a
//
