LOCUS       NM_001356466            3015 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            V (Pigv), transcript variant 4, mRNA.
ACCESSION   NM_001356466 XM_006538747
VERSION     NM_001356466.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Rodriguez de Los Santos,M., Rivalan,M., David,F.S., Stumpf,A.,
            Pitsch,J., Tsortouktzidis,D., Velasquez,L.M., Voigt,A.,
            Schilling,K., Mattei,D., Long,M., Vogt,G., Knaus,A.,
            Fischer-Zirnsak,B., Wittler,L., Timmermann,B., Robinson,P.N.,
            Horn,D., Mundlos,S., Kornak,U., Becker,A.J., Schmitz,D., Winter,Y.
            and Krawitz,P.M.
  TITLE     A CRISPR-Cas9-engineered mouse model for GPI-anchor deficiency
            mirrors human phenotypes and exhibits hippocampal synaptic
            dysfunctions
  JOURNAL   Proc Natl Acad Sci U S A 118 (2) (2021)
   PUBMED   33402532
  REMARK    GeneRIF: A CRISPR-Cas9-engineered mouse model for GPI-anchor
            deficiency mirrors human phenotypes and exhibits hippocampal
            synaptic dysfunctions.
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Li,Y., Klena,N.T., Gabriel,G.C., Liu,X., Kim,A.J., Lemke,K.,
            Chen,Y., Chatterjee,B., Devine,W., Damerla,R.R., Chang,C., Yagi,H.,
            San Agustin,J.T., Thahir,M., Anderton,S., Lawhead,C., Vescovi,A.,
            Pratt,H., Morgan,J., Haynes,L., Smith,C.L., Eppig,J.T.,
            Reinholdt,L., Francis,R., Leatherbury,L., Ganapathiraju,M.K.,
            Tobita,K., Pazour,G.J. and Lo,C.W.
  TITLE     Global genetic analysis in mice unveils central role for cilia in
            congenital heart disease
  JOURNAL   Nature 521 (7553), 520-524 (2015)
   PUBMED   25807483
REFERENCE   3  (bases 1 to 3015)
  AUTHORS   Kang,J.Y., Hong,Y., Ashida,H., Shishioh,N., Murakami,Y.,
            Morita,Y.S., Maeda,Y. and Kinoshita,T.
  TITLE     PIG-V involved in transferring the second mannose in
            glycosylphosphatidylinositol
  JOURNAL   J Biol Chem 280 (10), 9489-9497 (2005)
   PUBMED   15623507
REFERENCE   4  (bases 1 to 3015)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX537327.7, BY104545.1 and AK046527.1.
            
            On Oct 5, 2017 this sequence version replaced XM_006538747.3.
            
            Transcript Variant: This variant (4) differs in the 5' UTR compared
            to variant 1. Variants 1 and 4 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660819.27896.1,
                                           ERR3765653.2428809.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-90                BX537327.7         57179-57268         c
            91-305              BY104545.1         126-340
            306-403             BX537327.7         56866-56963         c
            404-2185            AK046527.1         291-2072
            2186-3015           BX537327.7         45527-46356         c
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 66.25 cM"
     gene            1..3015
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class V"
                     /db_xref="GeneID:230801"
                     /db_xref="MGI:MGI:2442480"
     exon            1..403
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            404..526
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             449..1930
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /EC_number="2.3.1.225"
                     /note="isoform 1 is encoded by transcript variant 4;
                     phosphatidylinositol glycan, class V; GPI
                     mannosyltransferase 2; PIG-V; GPI-MT-II; GPI
                     mannosyltransferase II; phosphatidylinositol-glycan
                     biosynthesis class V protein"
                     /codon_start=1
                     /product="GPI mannosyltransferase 2 isoform 1"
                     /protein_id="NP_001343395.1"
                     /db_xref="CCDS:CCDS18755.1"
                     /db_xref="GeneID:230801"
                     /db_xref="MGI:MGI:2442480"
                     /translation="MGLLDPSQKEVLRFAVNCRILTLVLQALFNLIIPDHHADAFCPP
                     RLAPSGSADQLVEGLLGGLSRWDAEHFLFIAEHGYLYEHNFAFFPGFPLALLMGTELL
                     RPLQGLLSQRSCLLVSVALLNLLFSVLAAVALHDLGCLVLHCPRQALCAALLFCISPA
                     NVFLAAGYSEALFAFLTFSAMGQLERGRGWASGLLFALAAGVRSNGLVSLGFLLHSQC
                     RGFCSSLAVLSPWKPLVKLMASVCLSVLIVSLPFALFQYRAYIQFCSPGSAPSIPEPL
                     LQLAADKGYRLAGENAPPWCSWDLPLIYNYIQDVYWNVGLLRYYELKQVPNFLLATPV
                     TVLVVWATWTYVTTHPWLCLTLGLQRTKDRENPEKPHRGFLSPKVFVYLVHAAALLVF
                     GGLCMHVQVLTRFLASSTPIMYWFPAHLLQDQEPLLRCVDTEPGKLPQEKSPPGQKAP
                     RNCLMKLFYDWKRCSPVTRCVLVYFLTYWLLGLILHCNFLPWT"
     exon            527..1648
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..3015
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2189
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
     regulatory      2994..2999
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3015
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /note="major polyA site"
ORIGIN      
        1 tccttcctcg tcccatacag tcatctgagg tgggacagag gactgtcgca gggcagaggt
       61 ccttcattgc caggagggga aaggcagggg gaattcccct actttcttct catccagttg
      121 cagggtaccg ctgcttgcca caaaaacccc aagaaggctt tcttttgcct attcatattg
      181 tctatcgtgt gccaggtcct gtgctgagct ggggtggcgg aacaccgaga gaacgcgtgg
      241 ctctggggca gcgcccgggt ccagcctcgg ctcacaccgg gaagtcagag gctgctcacg
      301 gcaggggact ggggtgagag ccccgacccg ggagcgcagc gcccgcgcac aaactgctgg
      361 cgagtcaaga ggcctactcc tcaggtgtca ccacgccggg aagaatttag aagccggagg
      421 aagctcagtc ctggtggtga gagagaggat gggactcctg gacccatccc agaaagaggt
      481 gctgagattc gcagtaaact gccgcatcct gactctagtg ctacaggcac tcttcaacct
      541 catcatccca gatcaccacg cagatgcctt ttgccctccc cgcctcgccc cctcgggctc
      601 tgcggatcag cttgtggaag gtcttctggg tggtctgtct cgatgggatg ctgaacactt
      661 cctgtttatc gctgagcacg gctaccttta tgagcacaac tttgccttct tccctggctt
      721 tcccttggcc ctgctgatgg ggactgagct gctgagaccc ttgcagggcc tcttgagcca
      781 gcgcagttgc ctgctggtct cagtagcact tctcaacttg ctgttctccg tgctggctgc
      841 agtggcactc catgacttgg gttgtctggt tctgcactgt cctcggcagg ccttgtgtgc
      901 agcgttgctc ttctgcatca gtcctgccaa cgttttcctg gcagccggct actcagaggc
      961 tttgtttgcc ttcctgacgt tcagcgccat ggggcagctg gagaggggcc gtggctgggc
     1021 tagtgggctg ctctttgctc ttgctgctgg ggtgcgctcc aatgggctgg tgagccttgg
     1081 cttcctcctg cactcccagt gcagaggctt ttgttcctct ctggcggtgc tgagcccctg
     1141 gaagcccctc gtgaagctga tggcctctgt gtgcttgtca gtgctcatag tcagccttcc
     1201 cttcgccctc tttcagtatc gtgcctacat ccagttctgc tcaccaggct cagccccctc
     1261 catccctgag cccttgctgc agctagctgc cgacaaaggc taccggcttg caggagaaaa
     1321 tgcaccccca tggtgctcct gggaccttcc tctaatatac aactatattc aggatgtcta
     1381 ctggaatgtt ggtcttctgc gatactatga gctcaaacag gtgcccaatt tcctactagc
     1441 tacaccagtg acagtactgg ttgtgtgggc aacctggaca tatgtgacca cccacccttg
     1501 gctctgcctt acacttgggc tgcaaagaac caaggacagg gagaacccgg agaagcccca
     1561 ccgtggcttc ctgagtccga aggtgtttgt gtacctggtc cacgctgcag cactgctggt
     1621 ctttggaggt ctgtgcatgc atgtccaggt tctcacgcgg tttttggcct cttccactcc
     1681 tatcatgtac tggtttccag ctcacctgct tcaggatcaa gaaccgctgc tgaggtgtgt
     1741 ggacacagag cctgggaagc ttcctcagga gaagtcccca ccaggacaga aggcccccag
     1801 aaactgtctc atgaaactct tctacgattg gaagagatgc tctccagtca caagatgcgt
     1861 tctggtctac ttcctgacct actggcttct gggactaatt ctccattgca acttcctgcc
     1921 ttggacttga cctggagtgt caaggaatga aagctctaag acgacaccct ggctgggaca
     1981 gcccgcacac tccggagagc actgtgcagt aggatctctg ggtctctctg ggggccagct
     2041 aagaagggaa aatgggagca caggcgcccc ttctcatcct gggcggggca ggtgtagggg
     2101 agtaacttca gtgctcagtg caggggtctc agatctccct agtccacaag tactaggcaa
     2161 tcttaaacct actactgatc cacgtgtaag tctgtcttct tgttttttga gagaaggttg
     2221 gtttctctac attgccctgg ttgtcctgga actcactctg tagaccaagt ttatgtagac
     2281 caggctggcc ttgaactcag agattcatct gcctctgcct cttgagtact aggcccacca
     2341 ctgagtgtga gccacccctg cctggcctgg tggataaatt taaatgtttg atcaaagcac
     2401 aggccaacag cctgccccac tcaggatggt ggagtcagtg cagtgtagtg acagaaggaa
     2461 gaggggacag acagcctgct ctaagtgaga ggtttctgaa ggtggttgtt ttcttttttt
     2521 ttttttttaa agatgtatta tattatatat gagtacacta gctgtcttca gacacaccag
     2581 aagagggcat cagatcccat tgcagatggt tgtgagccac catgtggctg ctgggaattg
     2641 aactcaggac ctctggaaga gcagccagtg ctcttaacca ctgagccatc tctccagctc
     2701 cctgaagttt tctttatgat actggtgtgt gtgtgtctgt gtctatccta cagactgtga
     2761 aaatctaaat actccttttg caatgaatta cctttttctg gaggaggggt agtcagggga
     2821 gctaagacaa ggtccctctg tgtaactctg actgtcctag aacttgcttg ctatgtagat
     2881 ctggctggtt ttgaactcag agatcctcct acctctgccg aactagtttc tataaattat
     2941 agagggttga ggctagaact ccgtagtaaa gcacacgagg ccctaatttc agtaataaac
     3001 accacttgga tcaga
//
