LOCUS       NM_001145956            1819 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            V (Pigv), transcript variant 3, mRNA.
ACCESSION   NM_001145956
VERSION     NM_001145956.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Rodriguez de Los Santos,M., Rivalan,M., David,F.S., Stumpf,A.,
            Pitsch,J., Tsortouktzidis,D., Velasquez,L.M., Voigt,A.,
            Schilling,K., Mattei,D., Long,M., Vogt,G., Knaus,A.,
            Fischer-Zirnsak,B., Wittler,L., Timmermann,B., Robinson,P.N.,
            Horn,D., Mundlos,S., Kornak,U., Becker,A.J., Schmitz,D., Winter,Y.
            and Krawitz,P.M.
  TITLE     A CRISPR-Cas9-engineered mouse model for GPI-anchor deficiency
            mirrors human phenotypes and exhibits hippocampal synaptic
            dysfunctions
  JOURNAL   Proc Natl Acad Sci U S A 118 (2) (2021)
   PUBMED   33402532
  REMARK    GeneRIF: A CRISPR-Cas9-engineered mouse model for GPI-anchor
            deficiency mirrors human phenotypes and exhibits hippocampal
            synaptic dysfunctions.
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Li,Y., Klena,N.T., Gabriel,G.C., Liu,X., Kim,A.J., Lemke,K.,
            Chen,Y., Chatterjee,B., Devine,W., Damerla,R.R., Chang,C., Yagi,H.,
            San Agustin,J.T., Thahir,M., Anderton,S., Lawhead,C., Vescovi,A.,
            Pratt,H., Morgan,J., Haynes,L., Smith,C.L., Eppig,J.T.,
            Reinholdt,L., Francis,R., Leatherbury,L., Ganapathiraju,M.K.,
            Tobita,K., Pazour,G.J. and Lo,C.W.
  TITLE     Global genetic analysis in mice unveils central role for cilia in
            congenital heart disease
  JOURNAL   Nature 521 (7553), 520-524 (2015)
   PUBMED   25807483
REFERENCE   3  (bases 1 to 1819)
  AUTHORS   Kang,J.Y., Hong,Y., Ashida,H., Shishioh,N., Murakami,Y.,
            Morita,Y.S., Maeda,Y. and Kinoshita,T.
  TITLE     PIG-V involved in transferring the second mannose in
            glycosylphosphatidylinositol
  JOURNAL   J Biol Chem 280 (10), 9489-9497 (2005)
   PUBMED   15623507
REFERENCE   4  (bases 1 to 1819)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK080590.1 and BX537327.7.
            
            Transcript Variant: This variant (3) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (3) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148285.1, AK080590.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-990               AK080590.1         2-991
            991-1819            BX537327.7         45527-46355         c
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 66.25 cM"
     gene            1..1819
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class V"
                     /db_xref="GeneID:230801"
                     /db_xref="MGI:MGI:2442480"
     exon            1..329
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            330..452
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             375..734
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /EC_number="2.3.1.225"
                     /note="isoform 3 is encoded by transcript variant 3;
                     phosphatidylinositol glycan, class V; GPI
                     mannosyltransferase 2; PIG-V; GPI-MT-II; GPI
                     mannosyltransferase II; phosphatidylinositol-glycan
                     biosynthesis class V protein"
                     /codon_start=1
                     /product="GPI mannosyltransferase 2 isoform 3"
                     /protein_id="NP_001139428.1"
                     /db_xref="CCDS:CCDS51322.1"
                     /db_xref="GeneID:230801"
                     /db_xref="MGI:MGI:2442480"
                     /translation="MGLLDPSQKEVLRFAVNCRILTLVLQVLTRFLASSTPIMYWFPA
                     HLLQDQEPLLRCVDTEPGKLPQEKSPPGQKAPRNCLMKLFYDWKRCSPVTRCVLVYFL
                     TYWLLGLILHCNFLPWT"
     exon            453..1819
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      993
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
     regulatory      1798..1803
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1819
                     /gene="Pigv"
                     /gene_synonym="B330013B03; D430024F16Rik"
                     /note="major polyA site"
ORIGIN      
        1 gagccggcgc gcaggcgcgg cacggacggg acccggatgg aggcgctgaa gttggagctc
       61 cgcgggggtg gtctggacgc gcgggcgcgg aggatccggc gccagctctg caactctgga
      121 gtcctccggg gagggaagct gtcaaacgca ggccgagaga aattaggcgg aggctgtaga
      181 gaagcgagga gccccgcccc tccgccatag cagcctccgg cgccgcctct tcctttgcag
      241 cctccggccc tcgtctgctt ccggccccgt tgcctggccc ggccccgcga ttcccccggg
      301 acgaagccct gggccgtggc tcccttggga atttagaagc cggaggaagc tcagtcctgg
      361 tggtgagaga gaggatggga ctcctggacc catcccagaa agaggtgctg agattcgcag
      421 taaactgccg catcctgact ctagtgctac aggttctcac gcggtttttg gcctcttcca
      481 ctcctatcat gtactggttt ccagctcacc tgcttcagga tcaagaaccg ctgctgaggt
      541 gtgtggacac agagcctggg aagcttcctc aggagaagtc cccaccagga cagaaggccc
      601 ccagaaactg tctcatgaaa ctcttctacg attggaagag atgctctcca gtcacaagat
      661 gcgttctggt ctacttcctg acctactggc ttctgggact aattctccat tgcaacttcc
      721 tgccttggac ttgacctgga gtgtcaagga atgaaagctc taagacgaca ccctggctgg
      781 gacagcccgc acactccgga gagcactgtg cagtaggatc tctgggtctc tctgggggcc
      841 agctaagaag ggaaaatggg agcacaggcg ccccttctca tcctgggcgg ggcaggtgta
      901 ggggagtaac ttcagtgctc agtgcagggg tctcagatct ccctagtcca caagtactag
      961 gcaatcttaa acctactact gatccacgtg taagtctgtc ttcttgtttt ttgagagaag
     1021 gttggtttct ctacattgcc ctggttgtcc tggaactcac tctgtagacc aagtttatgt
     1081 agaccaggct ggccttgaac tcagagattc atctgcctct gcctcttgag tactaggccc
     1141 accactgagt gtgagccacc cctgcctggc ctggtggata aatttaaatg tttgatcaaa
     1201 gcacaggcca acagcctgcc ccactcagga tggtggagtc agtgcagtgt agtgacagaa
     1261 ggaagagggg acagacagcc tgctctaagt gagaggtttc tgaaggtggt tgttttcttt
     1321 tttttttttt ttaaagatgt attatattat atatgagtac actagctgtc ttcagacaca
     1381 ccagaagagg gcatcagatc ccattgcaga tggttgtgag ccaccatgtg gctgctggga
     1441 attgaactca ggacctctgg aagagcagcc agtgctctta accactgagc catctctcca
     1501 gctccctgaa gttttcttta tgatactggt gtgtgtgtgt ctgtgtctat cctacagact
     1561 gtgaaaatct aaatactcct tttgcaatga attacctttt tctggaggag gggtagtcag
     1621 gggagctaag acaaggtccc tctgtgtaac tctgactgtc ctagaacttg cttgctatgt
     1681 agatctggct ggttttgaac tcagagatcc tcctacctct gccgaactag tttctataaa
     1741 ttatagaggg ttgaggctag aactccgtag taaagcacac gaggccctaa tttcagtaat
     1801 aaacaccact tggatcaga
//
