LOCUS       NM_133779               2541 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            T (Pigt), transcript variant 1, mRNA.
ACCESSION   NM_133779 XM_912976 XM_922560 XM_922575 XM_922583 XM_922591
            XM_922599
VERSION     NM_133779.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Li,H.L., Li,Z., Qin,L.Y., Liu,S., Lau,L.T., Han,J.S. and Yu,A.C.
  TITLE     The novel neurotrophin-regulated neuronal development-associated
            protein, NDAP, mediates apoptosis
  JOURNAL   FEBS Lett 580 (7), 1723-1728 (2006)
   PUBMED   16516892
  REMARK    GeneRIF: different regulations of NDAP expression by neurotrophins
            indicate that the expression of NDAP might be a checkpoint for
            apoptosis during neuronal development [NDAP]
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Li,Z., Li,Q., Sun,C.X., Hertz,L. and Yu,A.C.
  TITLE     Cloning and identification of differentially expressed transcripts
            in primary culture of GABAergic neurons
  JOURNAL   Neurochem Res 26 (10), 1101-1105 (2001)
   PUBMED   11700951
REFERENCE   3  (bases 1 to 2541)
  AUTHORS   Ohishi,K., Inoue,N. and Kinoshita,T.
  TITLE     PIG-S and PIG-T, essential for GPI anchor attachment to proteins,
            form a complex with GAA1 and GPI8
  JOURNAL   EMBO J 20 (15), 4088-4098 (2001)
   PUBMED   11483512
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591478.19.
            
            On May 27, 2022 this sequence version replaced NM_133779.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK155057.1, AK153464.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-217               AL591478.19        50612-50828
            218-395             AL591478.19        50937-51114
            396-523             AL591478.19        52866-52993
            524-624             AL591478.19        53262-53362
            625-711             AL591478.19        53503-53589
            712-799             AL591478.19        54152-54239
            800-897             AL591478.19        54345-54442
            898-1063            AL591478.19        54556-54721
            1064-1264           AL591478.19        55549-55749
            1265-1430           AL591478.19        59454-59619
            1431-1514           AL591478.19        59721-59804
            1515-2541           AL591478.19        60346-61372
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 85.17 cM"
     gene            1..2541
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class T"
                     /db_xref="GeneID:78928"
                     /db_xref="MGI:MGI:1926178"
     exon            1..217
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..1767
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; GPI transamidase component PIG-T; neuronal
                     development-associated protein 7;
                     phosphatidylinositol-glycan biosynthesis class T protein"
                     /codon_start=1
                     /product="GPI transamidase component PIG-T isoform 1
                     precursor"
                     /protein_id="NP_598540.2"
                     /db_xref="CCDS:CCDS17038.2"
                     /db_xref="GeneID:78928"
                     /db_xref="MGI:MGI:1926178"
                     /translation="MAAAMPLGLPLRLLVLLLVGRGCCGCAEGPRDSLREELVITPLP
                     SGDVAATFQFRTRWDSDLQREGVSHYRLFPKALGQLISKYSLRELHLSFTQGFWRTRY
                     WGPPFLQAPSGAELWVWFQDTVTDVDKSWRELSNVLSGIFCASLNFIDATNTVTPTAS
                     FKPLGLANDTDDYFLRYAVLPREVVCTENLTPWKKLLPCSSKAGLSVLLKADRLFHTS
                     YHSQAVHIRPICRNAHCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMFSRTLTEAC
                     PLASQSLVYVDITGYSQDNETLEVSPPPTSTYQDVILGTRKTYAVYDLFDTAMINNSR
                     NLNIQLKWKRPPDNEALPVPFLHAQRYVSGYGLQKGELSTLLYNSHPYRAFPVLLLDV
                     VPWYLRLYVHTLTITSKGKENKPSYIHYQPAQDRQQPHLLEMLIQLPANSVTKVSIQF
                     ERALLKWTEYTPDPNHGFYVSPSVLSALVPSVVAAKPVDWEGSPLFNTLFPVSDGSSY
                     FVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNLLTRTFHIEEPKSGGLA
                     KRLANLIRRARGVPPL"
     exon            218..395
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            396..523
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            524..624
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            625..711
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            712..799
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            800..897
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            898..1063
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..1264
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1430
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1514
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..2541
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2521..2526
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /note="hexamer: AATAAA"
     polyA_site      2541
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /note="major polyA site"
ORIGIN      
        1 gcggaagcgg ccgcaggtat ggcggctgcc atgccgctcg gtttgccgtt gcggttactg
       61 gtgctgttgc tggtgggacg gggctgctgt ggctgcgcgg agggcccacg cgacagcctg
      121 cgagaagagc tcgttatcac tccactgcct tccggcgacg tggccgccac attccagttc
      181 cgcacgcgtt gggattcgga tctgcagcgg gaaggagtgt cccattacag gctcttccct
      241 aaagccctgg gacagctgat ctccaagtac tccctgcggg agctccacct gtcgttcacg
      301 caaggctttt ggaggacgcg atactggggg ccacccttcc tgcaggctcc gtcaggtgca
      361 gagctctggg tctggttcca agacactgtc accgatgtgg ataagtcctg gagggagctc
      421 agcaatgtcc tctcggggat cttctgtgca tccctcaact tcatcgacgc caccaatacc
      481 gtcactccca ccgcctcctt caaacctctg ggactggcca atgacactga cgactacttc
      541 ctgcgctacg ctgtgctgcc tcgggaggtt gtctgcactg agaacctcac gccatggaag
      601 aagctcttgc cctgcagttc caaggccggt ctctcagtgc tattgaaagc agaccgcttg
      661 ttccacacca gttaccactc ccaggcagtg cacatccggc ctatctgcag aaatgctcac
      721 tgtaccagca tctcctggga gctgaggcag accctttcgg ttgtctttga tgccttcatc
      781 acaggacagg ggaagaaaga ctggtccctc ttccgcatgt tctcccggac tctcacagag
      841 gcctgtccat tggcgtctca gagcctagtt tatgtggaca tcacaggcta cagccaggat
      901 aatgaaacac tggaggtgag ccctccccca acttccacat accaggatgt cattttgggc
      961 accaggaaga cctatgccgt ctatgacttg tttgacacag ccatgatcaa taactctcga
     1021 aacctcaaca tccagctcaa atggaagaga cccccagata atgaggccct acccgtgccc
     1081 ttcctgcatg cgcagcggta cgtgagtggc tatggactgc agaagggaga gctgagtact
     1141 ctgctgtaca actcccatcc ttatcgggcc ttccccgtgc tgctgctgga tgtcgtgccc
     1201 tggtacctgc ggctgtatgt gcacacgctc accattacct ccaagggcaa ggagaataaa
     1261 ccaagttaca tccactacca acctgctcag gacaggcagc agccccacct cctggagatg
     1321 ctcattcagc tgccagccaa ctccgtcacc aaggtctcca tccagtttga acgagccctg
     1381 ctcaagtgga cagagtacac accagacccc aaccacggct tctatgtcag cccatctgtc
     1441 ctcagcgccc ttgtgcccag tgtggtggca gccaaaccag tggactggga agggagccct
     1501 ctcttcaaca ccttgttccc ggtgtctgat ggctccagct actttgtccg actctacaca
     1561 gagcccttgc tagtgaacct gcccaccccg gacttcagca tgccctacaa tgtgatctgc
     1621 cttacgtgca cggtggtggc cgtgtgctat ggctccttct acaatctcct cacccgaacc
     1681 tttcacatcg aagagcccaa gtcgggcggc ctggccaagc ggctggctaa cctcatccgc
     1741 cgtgcccgtg gtgtgccccc tctctagtcc ggcgtgcccg tggtgtaccc cctctctagt
     1801 cctgtgagcc cgtggtgtac cccctctcta gtccggcgcg cccgtggtgt gccccctctc
     1861 tagtccggcg cgcccgtggt gtgccccctc tctagtccgg cgagcccgtg gtgtgccccc
     1921 tctctagtcc ggcgagcccg tggtgtgccc cctctctagt ccggcgtgcc cgtggtgtac
     1981 cccctctcta gtcctgtgag cccgtggtgt accccctctc tagtccggcg cgcccgtggt
     2041 gtgccccctc tctagtccgg cgctcccatg gtgtgccccc tctctagtcc ggcgctcccg
     2101 tggtgtgccc cctctctagt ccggcgagcc cgtggtgtgc cccctctcta gtcctccctt
     2161 tccccagcaa ctgcagctgc actcgtccga cccgagggag cagggcaggg ctttttctgc
     2221 cacccgctgt gtctccagag ttagcttttg aggctaactc cctctggagc tggtctgggc
     2281 caaggcgtgg tttccagtgt ggtagacttg agccaaatcc cttcccatcc tctcctgtgg
     2341 cagccgtgct tctgcctaca aggggctcgt gctgcagtat accggacagc ctagcgatgc
     2401 ttccgtcttt actggacatt ctgatggccc aggcaatggg cgcagtagag ctgtggtgct
     2461 gtttgggggt ggggtggggt ggggtggggt gggggtgagt gtctgtgaac ttactgatgt
     2521 aataaagtgg cagccttcct a
//
