LOCUS       NM_001362645            2631 bp    mRNA    linear   ROD 28-AUG-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            T (Pigt), transcript variant 3, mRNA.
ACCESSION   NM_001362645
VERSION     NM_001362645.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Li,H.L., Li,Z., Qin,L.Y., Liu,S., Lau,L.T., Han,J.S. and Yu,A.C.
  TITLE     The novel neurotrophin-regulated neuronal development-associated
            protein, NDAP, mediates apoptosis
  JOURNAL   FEBS Lett 580 (7), 1723-1728 (2006)
   PUBMED   16516892
  REMARK    GeneRIF: different regulations of NDAP expression by neurotrophins
            indicate that the expression of NDAP might be a checkpoint for
            apoptosis during neuronal development [NDAP]
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Li,Z., Li,Q., Sun,C.X., Hertz,L. and Yu,A.C.
  TITLE     Cloning and identification of differentially expressed transcripts
            in primary culture of GABAergic neurons
  JOURNAL   Neurochem Res 26 (10), 1101-1105 (2001)
   PUBMED   11700951
REFERENCE   3  (bases 1 to 2631)
  AUTHORS   Ohishi,K., Inoue,N. and Kinoshita,T.
  TITLE     PIG-S and PIG-T, essential for GPI anchor attachment to proteins,
            form a complex with GAA1 and GPI8
  JOURNAL   EMBO J 20 (15), 4088-4098 (2001)
   PUBMED   11483512
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591478.19.
            
            On May 27, 2022 this sequence version replaced NM_001362645.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (3) is
            shorter at the N-terminus compared to isoform 1.
            
            ##Evidence-Data-START##
            CDS exon combination :: SRR17784647.1397263.1, SRR13422597.711832.1
                                    [ECO:0000331]
            RNAseq introns       :: partial sample support SAMN00849374,
                                    SAMN00849375 [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-217               AL591478.19        50612-50828
            218-395             AL591478.19        50937-51114
            396-485             AL591478.19        51561-51650
            486-613             AL591478.19        52866-52993
            614-714             AL591478.19        53262-53362
            715-801             AL591478.19        53503-53589
            802-889             AL591478.19        54152-54239
            890-987             AL591478.19        54345-54442
            988-1153            AL591478.19        54556-54721
            1154-1354           AL591478.19        55549-55749
            1355-1520           AL591478.19        59454-59619
            1521-1604           AL591478.19        59721-59804
            1605-2631           AL591478.19        60346-61372
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 85.17 cM"
     gene            1..2631
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class T"
                     /db_xref="GeneID:78928"
                     /db_xref="MGI:MGI:1926178"
     exon            1..217
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            218..395
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            396..485
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            486..613
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            614..714
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            715..801
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            802..889
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            890..987
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     CDS             907..1857
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /note="isoform 3 is encoded by transcript variant 3; GPI
                     transamidase component PIG-T; neuronal
                     development-associated protein 7;
                     phosphatidylinositol-glycan biosynthesis class T protein"
                     /codon_start=1
                     /product="GPI transamidase component PIG-T isoform 3"
                     /protein_id="NP_001349574.1"
                     /db_xref="GeneID:78928"
                     /db_xref="MGI:MGI:1926178"
                     /translation="MFSRTLTEACPLASQSLVYVDITGYSQDNETLEVSPPPTSTYQD
                     VILGTRKTYAVYDLFDTAMINNSRNLNIQLKWKRPPDNEALPVPFLHAQRYVSGYGLQ
                     KGELSTLLYNSHPYRAFPVLLLDVVPWYLRLYVHTLTITSKGKENKPSYIHYQPAQDR
                     QQPHLLEMLIQLPANSVTKVSIQFERALLKWTEYTPDPNHGFYVSPSVLSALVPSVVA
                     AKPVDWEGSPLFNTLFPVSDGSSYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAV
                     CYGSFYNLLTRTFHIEEPKSGGLAKRLANLIRRARGVPPL"
     exon            988..1153
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1354
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1520
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1604
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..2631
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2611..2616
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /note="hexamer: AATAAA"
     polyA_site      2631
                     /gene="Pigt"
                     /gene_synonym="2510012P17Rik; 4930534E15Rik; CGI-06; NDAP;
                     Ndap7"
                     /note="major polyA site"
ORIGIN      
        1 gcggaagcgg ccgcaggtat ggcggctgcc atgccgctcg gtttgccgtt gcggttactg
       61 gtgctgttgc tggtgggacg gggctgctgt ggctgcgcgg agggcccacg cgacagcctg
      121 cgagaagagc tcgttatcac tccactgcct tccggcgacg tggccgccac attccagttc
      181 cgcacgcgtt gggattcgga tctgcagcgg gaaggagtgt cccattacag gctcttccct
      241 aaagccctgg gacagctgat ctccaagtac tccctgcggg agctccacct gtcgttcacg
      301 caaggctttt ggaggacgcg atactggggg ccacccttcc tgcaggctcc gtcaggtgca
      361 gagctctggg tctggttcca agacactgtc accgactatt tgcatcagta tgcatgcaca
      421 tacaacatga agaaattaca ttattattga gtgaacatcg tctcctgaaa acttccaagt
      481 ctgagtgtgg ataagtcctg gagggagctc agcaatgtcc tctcggggat cttctgtgca
      541 tccctcaact tcatcgacgc caccaatacc gtcactccca ccgcctcctt caaacctctg
      601 ggactggcca atgacactga cgactacttc ctgcgctacg ctgtgctgcc tcgggaggtt
      661 gtctgcactg agaacctcac gccatggaag aagctcttgc cctgcagttc caaggccggt
      721 ctctcagtgc tattgaaagc agaccgcttg ttccacacca gttaccactc ccaggcagtg
      781 cacatccggc ctatctgcag aaatgctcac tgtaccagca tctcctggga gctgaggcag
      841 accctttcgg ttgtctttga tgccttcatc acaggacagg ggaagaaaga ctggtccctc
      901 ttccgcatgt tctcccggac tctcacagag gcctgtccat tggcgtctca gagcctagtt
      961 tatgtggaca tcacaggcta cagccaggat aatgaaacac tggaggtgag ccctccccca
     1021 acttccacat accaggatgt cattttgggc accaggaaga cctatgccgt ctatgacttg
     1081 tttgacacag ccatgatcaa taactctcga aacctcaaca tccagctcaa atggaagaga
     1141 cccccagata atgaggccct acccgtgccc ttcctgcatg cgcagcggta cgtgagtggc
     1201 tatggactgc agaagggaga gctgagtact ctgctgtaca actcccatcc ttatcgggcc
     1261 ttccccgtgc tgctgctgga tgtcgtgccc tggtacctgc ggctgtatgt gcacacgctc
     1321 accattacct ccaagggcaa ggagaataaa ccaagttaca tccactacca acctgctcag
     1381 gacaggcagc agccccacct cctggagatg ctcattcagc tgccagccaa ctccgtcacc
     1441 aaggtctcca tccagtttga acgagccctg ctcaagtgga cagagtacac accagacccc
     1501 aaccacggct tctatgtcag cccatctgtc ctcagcgccc ttgtgcccag tgtggtggca
     1561 gccaaaccag tggactggga agggagccct ctcttcaaca ccttgttccc ggtgtctgat
     1621 ggctccagct actttgtccg actctacaca gagcccttgc tagtgaacct gcccaccccg
     1681 gacttcagca tgccctacaa tgtgatctgc cttacgtgca cggtggtggc cgtgtgctat
     1741 ggctccttct acaatctcct cacccgaacc tttcacatcg aagagcccaa gtcgggcggc
     1801 ctggccaagc ggctggctaa cctcatccgc cgtgcccgtg gtgtgccccc tctctagtcc
     1861 ggcgtgcccg tggtgtaccc cctctctagt cctgtgagcc cgtggtgtac cccctctcta
     1921 gtccggcgcg cccgtggtgt gccccctctc tagtccggcg cgcccgtggt gtgccccctc
     1981 tctagtccgg cgagcccgtg gtgtgccccc tctctagtcc ggcgagcccg tggtgtgccc
     2041 cctctctagt ccggcgtgcc cgtggtgtac cccctctcta gtcctgtgag cccgtggtgt
     2101 accccctctc tagtccggcg cgcccgtggt gtgccccctc tctagtccgg cgctcccatg
     2161 gtgtgccccc tctctagtcc ggcgctcccg tggtgtgccc cctctctagt ccggcgagcc
     2221 cgtggtgtgc cccctctcta gtcctccctt tccccagcaa ctgcagctgc actcgtccga
     2281 cccgagggag cagggcaggg ctttttctgc cacccgctgt gtctccagag ttagcttttg
     2341 aggctaactc cctctggagc tggtctgggc caaggcgtgg tttccagtgt ggtagacttg
     2401 agccaaatcc cttcccatcc tctcctgtgg cagccgtgct tctgcctaca aggggctcgt
     2461 gctgcagtat accggacagc ctagcgatgc ttccgtcttt actggacatt ctgatggccc
     2521 aggcaatggg cgcagtagag ctgtggtgct gtttgggggt ggggtggggt ggggtggggt
     2581 gggggtgagt gtctgtgaac ttactgatgt aataaagtgg cagccttcct a
//
