LOCUS       NM_001413274            3060 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            Q (Pigq), transcript variant 4, mRNA.
ACCESSION   NM_001413274
VERSION     NM_001413274.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   3  (bases 1 to 3060)
  AUTHORS   Watanabe,R., Murakami,Y., Marmor,M.D., Inoue,N., Maeda,Y., Hino,J.,
            Kangawa,K., Julius,M. and Kinoshita,T.
  TITLE     Initial enzyme for glycosylphosphatidylinositol biosynthesis
            requires PIG-P and is regulated by DPM2
  JOURNAL   EMBO J 19 (16), 4402-4411 (2000)
   PUBMED   10944123
REFERENCE   4  (bases 1 to 3060)
  AUTHORS   Hong,Y., Ohishi,K., Watanabe,R., Endo,Y., Maeda,Y. and Kinoshita,T.
  TITLE     GPI1 stabilizes an enzyme essential in the first step of
            glycosylphosphatidylinositol biosynthesis
  JOURNAL   J Biol Chem 274 (26), 18582-18588 (1999)
   PUBMED   10373468
REFERENCE   5  (bases 1 to 3060)
  AUTHORS   Tiede,A., Schubert,J., Nischan,C., Jensen,I., Westfall,B.,
            Taron,C.H., Orlean,P. and Schmidt,R.E.
  TITLE     Human and mouse Gpi1p homologues restore
            glycosylphosphatidylinositol membrane anchor biosynthesis in yeast
            mutants
  JOURNAL   Biochem J 334 (Pt 3) (Pt 3), 609-616 (1998)
   PUBMED   9729469
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159277.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14777534.1027315.1,
                                           SRR17784648.212106.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164140
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               AC159277.3         91433-91553
            122-820             AC159277.3         95906-96604
            821-952             AC159277.3         98234-98365
            953-1073            AC159277.3         98490-98610
            1074-1200           AC159277.3         101137-101263
            1201-1360           AC159277.3         101541-101700
            1361-1472           AC159277.3         101795-101906
            1473-1553           AC159277.3         102330-102410
            1554-1668           AC159277.3         102733-102847
            1669-1730           AC159277.3         104516-104577
            1731-3060           AC159277.3         105612-106941
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 13.01 cM"
     gene            1..3060
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class Q"
                     /db_xref="GeneID:14755"
                     /db_xref="MGI:MGI:1333114"
     exon            1..121
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            122..820
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..1883
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /EC_number="2.4.1.198"
                     /note="isoform 4 is encoded by transcript variant 4;
                     phosphatidylinositol N-acetylglucosaminyltransferase
                     subunit Q; PIG-Q; N-acetylglucosamyl transferase component
                     GPI1; phosphatidylinositol-glycan biosynthesis class Q
                     protein"
                     /codon_start=1
                     /product="phosphatidylinositol
                     N-acetylglucosaminyltransferase subunit Q isoform 4"
                     /protein_id="NP_001400203.1"
                     /db_xref="GeneID:14755"
                     /db_xref="MGI:MGI:1333114"
                     /translation="MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQ
                     VKELLAQVQKASQVPVAVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGT
                     RLWSCKATYPQMSNPLDMHPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTG
                     GLADIFDTVARSEVLFRNDQFDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLAS
                     LLSLISAASACRLWKLWPLSFIRSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKA
                     NMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAPAGL
                     KMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDII
                     ALLTFHIYCFYVYGARYVLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFI
                     GTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCRP
                     YRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHVMHIYRLPRCGCHPKHSWGTLCRKL
                     VFGELIYPWRQREDKQD"
     exon            821..952
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1073
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1200
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1360
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1472
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1553
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1668
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1730
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..3060
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3038..3043
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /note="hexamer: AATAAA"
     polyA_site      3060
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /note="major polyA site"
ORIGIN      
        1 gctgatgtcc cgcggcgggc ctgggagccg caagcacggt cgtcctcccg ggcccgccga
       61 acgcagtgcc caacccagtt ccttccctac cgctcgggga cgcggccccg gggaccagct
      121 gggcccccag catggtgctc aaggtcttct tccccacgtg ctgtgcctcg gcagacagtg
      181 gcctgttagt gggacgatgg gttccaggac agagcagtgc tgtgatcctg gccgtggtgc
      241 actttccctt catccccatc caagtcaagg agctcctggc ccaggtgcag aaggccagtc
      301 aagtaccagt ggctgtgcta gggacctggt gccaccgtca gcaggaaccg caggagagcc
      361 tggggaattt cctggagggc ctgggcacca tcttctccca tgacccctgg ttgcagctct
      421 gccgggagag gggtaccaga ctctggagct gcaaggccac ttacccgcag atgtctaacc
      481 ctctggacat gcaccccgaa gagcaggtca tgctcatctt ctatgatcag cgcaagctgc
      541 tgctctcctg gttgcatccg cctccagtcc tgcctgcctg ccagatggga gacaccacag
      601 ccagcaccgg aggcctagct gacatctttg acacagtggc acgcagcgag gtgctcttcc
      661 gaaatgacca atttgatgag agacccgtac gcctgagcca ctggcaatca gagggagtgg
      721 aggccagcat acttgtggag ttggcaaagc gggcctctgg gcctgtctgc ctgctgctgg
      781 cttctctgtt gtccctgatc tcagcagcta gtgcttgccg gctatggaag ctgtggccac
      841 tttccttcat cagaagcaag ctctccactt gtgagcagct ccatcaccgg ctgaagcacc
      901 tctccttcat cttcagcacg gagaaagccc agaatcccat gcagctgatg aggaaggcca
      961 acatgctggt ttctgtgcta ctggatgtgg cccttggctt gctgctgctc tcttggctcc
     1021 acagcaataa ccgaattgga cagctggcca atgccctggt ccctgtggct gatcgcgtgg
     1081 ctgaggagct ccagcatctg ctgcagtggc tgatgggtgc tcctgctggg ctcaagatga
     1141 atcgggcact ggatcaggtg ctaggccgct ttttcctgta ccacatccat ctgtggataa
     1201 gctatatcca ccttatgtcc ccctttattg agcatatcct gtggcatgtg ggactctcag
     1261 cctgccttgg actgactgtt gctctgtcca tcttttcgga tatcatcgcc cttctcacct
     1321 tccacatcta ttgcttctat gtctacggtg ccaggtatgt gctgtactgc ctgaagatct
     1381 atggcctttc ctctctctgg cgtctgttcc gggggaagaa gtggaatgtt ctgcgccagc
     1441 gagtggattc ttgttcctat gaccttgacc agctcttcat tgggaccttg ctcttcacca
     1501 tcctggtctt cttgctaccc accaccgctc tgtactacct ggtattcacc ctgctccggc
     1561 tcctggtgat cactgtgcaa ggcttaatcc atctacttgt agacctcatc aattccctgc
     1621 cactatactc ccttggcctt cgactctgcc gaccctacag gctggcagct ggtgtgaagt
     1681 tcagagttct ggaaaaggag gcaggcagac ccctccgcct cctgatgcag ataaaccccc
     1741 tgtcctataa ccatgtgatg cacatctacc gtctgcctcg ctgtggctgc catcccaagc
     1801 actcctgggg taccctgtgc cgaaagctgg tcttcggaga gctcatctac ccctggaggc
     1861 agagagagga caaacaggac tgaggagctg gacacagcct acccatagct actgcacaac
     1921 catcacgcct cctctctgcc aggacagcac tgaccacggg gtcatctcag gtcccacaag
     1981 ggccaaccaa acactctgtg tgctgaatgt aggcaggcct tacccttgag ttactacttt
     2041 gctctcggac cctgccagct tcagtcctgc actctatcta gcacagtggt tctcaaccgt
     2101 cccaaagctg accctttaat acagttcctc atgttgtggt gaccccaacc ataaaattat
     2161 attcattact acttcattac tgttaatttt gctactgcta tgaatcatga tataatataa
     2221 atatctgtgt tttctgacag tcataggtga accctgtgaa agtgtcatat gacacccacc
     2281 ccaaagggtt gagaaccact gctctagtat cacctatgta gggacccaca tggtttttgt
     2341 agtcattaca gtgagcctga ttcctaggag ttcccggacc ccgtgggata tgctggcagg
     2401 tgctcttggc tgctttattg ggtgggagca ctcctgggca cctggtgtac ttcataagcc
     2461 tccgcagaac atttccattg ggaaacaggc agctgctgat cccttagccc ctcgaactaa
     2521 tccctcaccc tgtacttcat tgggcttact tgctactctg gagattgctt tgtgaagttg
     2581 ctgttaatag gcctttgggt accctttgct tgaagttata tcaactgagg tgattggata
     2641 cctgccaacc tggctagtgc tccatgtatg aataggccct ttggcaaaat attctatgtt
     2701 ctcttataga ctccttatgg gaccagatag cttccttctg gacaggcact tcatggctca
     2761 ttgtgtgaat gatcttgggg tggtgaaatg gccagggttg gttccctaac acttagtggg
     2821 accccatgtg agctagtccc tagggggcag gaaacctcac tgcctgaatt gtctactgtg
     2881 ggcaggaggg gacagccggc ccagcctgtc tttcaggggc acttgggact attgacattg
     2941 gtctgtctgt gcctgcttcc tcttgctata ggtcacagcc actcttaagc agccagtgga
     3001 gggttttggt gaagatgaat cctgatcctg tgatgacaat aaaaatctca tgaatctgga
//
