LOCUS       NM_001291025            3234 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            Q (Pigq), transcript variant 1, mRNA.
ACCESSION   NM_001291025 XM_006523663 XM_006523665
VERSION     NM_001291025.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   3  (bases 1 to 3234)
  AUTHORS   Watanabe,R., Murakami,Y., Marmor,M.D., Inoue,N., Maeda,Y., Hino,J.,
            Kangawa,K., Julius,M. and Kinoshita,T.
  TITLE     Initial enzyme for glycosylphosphatidylinositol biosynthesis
            requires PIG-P and is regulated by DPM2
  JOURNAL   EMBO J 19 (16), 4402-4411 (2000)
   PUBMED   10944123
REFERENCE   4  (bases 1 to 3234)
  AUTHORS   Hong,Y., Ohishi,K., Watanabe,R., Endo,Y., Maeda,Y. and Kinoshita,T.
  TITLE     GPI1 stabilizes an enzyme essential in the first step of
            glycosylphosphatidylinositol biosynthesis
  JOURNAL   J Biol Chem 274 (26), 18582-18588 (1999)
   PUBMED   10373468
REFERENCE   5  (bases 1 to 3234)
  AUTHORS   Tiede,A., Schubert,J., Nischan,C., Jensen,I., Westfall,B.,
            Taron,C.H., Orlean,P. and Schmidt,R.E.
  TITLE     Human and mouse Gpi1p homologues restore
            glycosylphosphatidylinositol membrane anchor biosynthesis in yeast
            mutants
  JOURNAL   Biochem J 334 (Pt 3) (Pt 3), 609-616 (1998)
   PUBMED   9729469
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159277.3.
            
            On Oct 29, 2022 this sequence version replaced NM_001291025.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform 1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK170431.1, ERR3363658.5615429.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               AC159277.3         91433-91553
            122-820             AC159277.3         95906-96604
            821-952             AC159277.3         98234-98365
            953-1073            AC159277.3         98490-98610
            1074-1253           AC159277.3         99095-99274
            1254-1380           AC159277.3         101137-101263
            1381-1534           AC159277.3         101541-101694
            1535-1646           AC159277.3         101795-101906
            1647-1727           AC159277.3         102330-102410
            1728-1842           AC159277.3         102733-102847
            1843-1904           AC159277.3         104516-104577
            1905-3234           AC159277.3         105612-106941
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 13.01 cM"
     gene            1..3234
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class Q"
                     /db_xref="GeneID:14755"
                     /db_xref="MGI:MGI:1333114"
     exon            1..121
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            122..820
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..2057
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /EC_number="2.4.1.198"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phosphatidylinositol N-acetylglucosaminyltransferase
                     subunit Q; PIG-Q; N-acetylglucosamyl transferase component
                     GPI1; phosphatidylinositol-glycan biosynthesis class Q
                     protein"
                     /codon_start=1
                     /product="phosphatidylinositol
                     N-acetylglucosaminyltransferase subunit Q isoform 1"
                     /protein_id="NP_001277954.1"
                     /db_xref="CCDS:CCDS70774.1"
                     /db_xref="GeneID:14755"
                     /db_xref="MGI:MGI:1333114"
                     /translation="MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQ
                     VKELLAQVQKASQVPVAVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGT
                     RLWSCKATYPQMSNPLDMHPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTG
                     GLADIFDTVARSEVLFRNDQFDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLAS
                     LLSLISAASACRLWKLWPLSFIRSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKA
                     NMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADSQSSVSVEVLWALFCSVSAD
                     ACWVCLLPGQESGAAVGSSQLCEICGGHPSRKEELELGERRVAEELQHLLQWLMGAPA
                     GLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSD
                     IIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFI
                     GTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCRP
                     YRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHVMHIYRLPRCGCHPKHSWGTLCRKL
                     VFGELIYPWRQREDKQD"
     exon            821..952
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1073
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1253
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1380
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1534
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1646
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1727
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1842
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..1904
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..3234
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3212..3217
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /note="hexamer: AATAAA"
     polyA_site      3234
                     /gene="Pigq"
                     /gene_synonym="Gpi1; Gpi1h; Gpi1p; Gpih"
                     /note="major polyA site"
ORIGIN      
        1 gctgatgtcc cgcggcgggc ctgggagccg caagcacggt cgtcctcccg ggcccgccga
       61 acgcagtgcc caacccagtt ccttccctac cgctcgggga cgcggccccg gggaccagct
      121 gggcccccag catggtgctc aaggtcttct tccccacgtg ctgtgcctcg gcagacagtg
      181 gcctgttagt gggacgatgg gttccaggac agagcagtgc tgtgatcctg gccgtggtgc
      241 actttccctt catccccatc caagtcaagg agctcctggc ccaggtgcag aaggccagtc
      301 aagtaccagt ggctgtgcta gggacctggt gccaccgtca gcaggaaccg caggagagcc
      361 tggggaattt cctggagggc ctgggcacca tcttctccca tgacccctgg ttgcagctct
      421 gccgggagag gggtaccaga ctctggagct gcaaggccac ttacccgcag atgtctaacc
      481 ctctggacat gcaccccgaa gagcaggtca tgctcatctt ctatgatcag cgcaagctgc
      541 tgctctcctg gttgcatccg cctccagtcc tgcctgcctg ccagatggga gacaccacag
      601 ccagcaccgg aggcctagct gacatctttg acacagtggc acgcagcgag gtgctcttcc
      661 gaaatgacca atttgatgag agacccgtac gcctgagcca ctggcaatca gagggagtgg
      721 aggccagcat acttgtggag ttggcaaagc gggcctctgg gcctgtctgc ctgctgctgg
      781 cttctctgtt gtccctgatc tcagcagcta gtgcttgccg gctatggaag ctgtggccac
      841 tttccttcat cagaagcaag ctctccactt gtgagcagct ccatcaccgg ctgaagcacc
      901 tctccttcat cttcagcacg gagaaagccc agaatcccat gcagctgatg aggaaggcca
      961 acatgctggt ttctgtgcta ctggatgtgg cccttggctt gctgctgctc tcttggctcc
     1021 acagcaataa ccgaattgga cagctggcca atgccctggt ccctgtggct gatagtcaaa
     1081 gcagtgtctc cgtggaagtc ttgtgggctc tcttctgctc cgtcagtgcg gatgcctgct
     1141 gggtctgttt gttgccaggc caggaatctg gggcagcagt gggctcctct cagctttgtg
     1201 agatctgtgg aggtcatccc agtaggaaag aggagctgga gctgggtgaa cggcgcgtgg
     1261 ctgaggagct ccagcatctg ctgcagtggc tgatgggtgc tcctgctggg ctcaagatga
     1321 atcgggcact ggatcaggtg ctaggccgct ttttcctgta ccacatccat ctgtggataa
     1381 gctatatcca ccttatgtcc ccctttattg agcatatcct gtggcatgtg ggactctcag
     1441 cctgccttgg actgactgtt gctctgtcca tcttttcgga tatcatcgcc cttctcacct
     1501 tccacatcta ttgcttctat gtctacggtg ccaggctgta ctgcctgaag atctatggcc
     1561 tttcctctct ctggcgtctg ttccggggga agaagtggaa tgttctgcgc cagcgagtgg
     1621 attcttgttc ctatgacctt gaccagctct tcattgggac cttgctcttc accatcctgg
     1681 tcttcttgct acccaccacc gctctgtact acctggtatt caccctgctc cggctcctgg
     1741 tgatcactgt gcaaggctta atccatctac ttgtagacct catcaattcc ctgccactat
     1801 actcccttgg ccttcgactc tgccgaccct acaggctggc agctggtgtg aagttcagag
     1861 ttctggaaaa ggaggcaggc agacccctcc gcctcctgat gcagataaac cccctgtcct
     1921 ataaccatgt gatgcacatc taccgtctgc ctcgctgtgg ctgccatccc aagcactcct
     1981 ggggtaccct gtgccgaaag ctggtcttcg gagagctcat ctacccctgg aggcagagag
     2041 aggacaaaca ggactgagga gctggacaca gcctacccat agctactgca caaccatcac
     2101 gcctcctctc tgccaggaca gcactgacca cggggtcatc tcaggtccca caagggccaa
     2161 ccaaacactc tgtgtgctga atgtaggcag gccttaccct tgagttacta ctttgctctc
     2221 ggaccctgcc agcttcagtc ctgcactcta tctagcacag tggttctcaa ccgtcccaaa
     2281 gctgaccctt taatacagtt cctcatgttg tggtgacccc aaccataaaa ttatattcat
     2341 tactacttca ttactgttaa ttttgctact gctatgaatc atgatataat ataaatatct
     2401 gtgttttctg acagtcatag gtgaaccctg tgaaagtgtc atatgacacc caccccaaag
     2461 ggttgagaac cactgctcta gtatcaccta tgtagggacc cacatggttt ttgtagtcat
     2521 tacagtgagc ctgattccta ggagttcccg gaccccgtgg gatatgctgg caggtgctct
     2581 tggctgcttt attgggtggg agcactcctg ggcacctggt gtacttcata agcctccgca
     2641 gaacatttcc attgggaaac aggcagctgc tgatccctta gcccctcgaa ctaatccctc
     2701 accctgtact tcattgggct tacttgctac tctggagatt gctttgtgaa gttgctgtta
     2761 ataggccttt gggtaccctt tgcttgaagt tatatcaact gaggtgattg gatacctgcc
     2821 aacctggcta gtgctccatg tatgaatagg ccctttggca aaatattcta tgttctctta
     2881 tagactcctt atgggaccag atagcttcct tctggacagg cacttcatgg ctcattgtgt
     2941 gaatgatctt ggggtggtga aatggccagg gttggttccc taacacttag tgggacccca
     3001 tgtgagctag tccctagggg gcaggaaacc tcactgcctg aattgtctac tgtgggcagg
     3061 aggggacagc cggcccagcc tgtctttcag gggcacttgg gactattgac attggtctgt
     3121 ctgtgcctgc ttcctcttgc tataggtcac agccactctt aagcagccag tggagggttt
     3181 tggtgaagat gaatcctgat cctgtgatga caataaaaat ctcatgaatc tgga
//
