LOCUS       NM_001305387            3539 bp    mRNA    linear   ROD 18-DEC-2022
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            O (Pigo), transcript variant 3, mRNA.
ACCESSION   NM_001305387
VERSION     NM_001305387.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H,
            Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T,
            Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P,
            Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ,
            Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD,
            Flicek P, Skarnes WC, Mallon AM and Parkinson H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Shishioh N, Hong Y, Ohishi K, Ashida H, Maeda Y and Kinoshita T.
  TITLE     GPI7 is the second partner of PIG-F and involved in modification of
            glycosylphosphatidylinositol
  JOURNAL   J Biol Chem 280 (10), 9728-9734 (2005)
   PUBMED   15632136
REFERENCE   3  (bases 1 to 3539)
  AUTHORS   Hong Y, Maeda Y, Watanabe R, Inoue N, Ohishi K and Kinoshita T.
  TITLE     Requirement of PIG-F and PIG-O for transferring phosphoethanolamine
            to the third mannose in glycosylphosphatidylinositol
  JOURNAL   J Biol Chem 275 (27), 20911-20919 (2000)
   PUBMED   10781593
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF532064.1, BY130204.1, BC092394.1 and BU708685.1.
            
            [WARNING] On Jul 26, 2023 this sequence was replaced by
            NM_001305387.2.
            
            Transcript Variant: This variant (3) differs in the 5' UTR, uses a
            downstream start codon, and uses an alternate in-frame splice site
            in 3' coding region compared to variant 1. The encoded isoform (3)
            has a shorter N-terminus and lacks an internal segment compared to
            isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036977.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                CF532064.1         239-264
            27-45               BY130204.1         17-35
            46-2696             BC092394.1         701-3351
            2697-3527           BC092394.1         3424-4254
            3528-3539           BU708685.1         485-496
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4"
     gene            1..3539
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class O"
                     /db_xref="GeneID:56703"
                     /db_xref="MGI:MGI:1861452"
     exon            1..45
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /inference="alignment:Splign:2.1.0"
     exon            46..557
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..3256
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /note="isoform 3 is encoded by transcript variant 3; GPI
                     ethanolamine phosphate transferase 3;
                     phosphatidylinositol-glycan biosynthesis class O protein"
                     /codon_start=1
                     /product="GPI ethanolamine phosphate transferase 3 isoform
                     3"
                     /protein_id="NP_001292316.1"
                     /db_xref="CCDS:CCDS84720.1"
                     /db_xref="GeneID:56703"
                     /db_xref="MGI:MGI:1861452"
                     /translation="MRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQEL
                     PGPGPLPWGSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLG
                     KLGSLQRILESQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVE
                     DNVIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPT
                     LDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAG
                     DHGMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLP
                     IPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVK
                     ELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLV
                     RMAGGAALLAAACLLCLLASQLAVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSK
                     IDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSF
                     VVAEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALW
                     LGIGLLLFTRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALL
                     VVVRLWLRRYGNLKSPEPPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGA
                     SAVLPRAVMGLAALGLVLLLWRPVTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVV
                     PQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSVYSAAMVTALLLLAFPLMLLHVERV
                     SLVFLLLFLQSFLLLHLLAAGTPVATPGHQPVFSAIHWHAAFVGFPDGHGSSTWLPAL
                     LVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEP
                     LMEMRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIF
                     EAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR"
     exon            558..701
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /inference="alignment:Splign:2.1.0"
     exon            702..825
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /inference="alignment:Splign:2.1.0"
     exon            826..985
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1168
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..2696
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /inference="alignment:Splign:2.1.0"
     exon            2697..2831
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /inference="alignment:Splign:2.1.0"
     exon            2832..3055
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /inference="alignment:Splign:2.1.0"
     exon            3056..3126
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /inference="alignment:Splign:2.1.0"
     exon            3127..3539
                     /gene="Pigo"
                     /gene_synonym="PIG-O"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagccctgcc gctgcacttc cgggcgcgga tccaggtgag ccaaggatgc ggaaggtgtc
       61 tgtgctcctc ttcttagcct gggtctgttt tcttttctac gctggcattg cactcttcac
      121 cagtggcttc cttctcactc gtctggagct caccaatcaa agcagctgcc aagagctccc
      181 aggccctggt cccttgccgt ggggaagtca tggaaagcct ggggcttgct ggatgccttc
      241 ccgcttctcc agggtggttt tggtactgat agacgctctg cggtttgact ttgcccagcc
      301 tcagcgctcg catgtgcctg gggaacctcc tgtctccgtg ccttttctgg gaaaattagg
      361 ttccttgcag aggatcctag agagtcaacc ccaccatggc cggctgtacc gatctcaggt
      421 ggaccccccc acgaccacca tgcagcgtct aaaggctctc accactggct cgctgccgac
      481 tttcattgat gctggcagta actttgccag ccatgctata gtggaagata atgtcatcca
      541 acagctcaac agtgcaggaa ggcgtgtagt cttcatggga gatgatacct ggagagacct
      601 cttccccgga gccttttctc aagctttctt cttctcatcc ttcaatgtca gagacctgca
      661 cacagtggat aacggcatct tggaacacct ctatcctacc ctggacggcg gttcgtggga
      721 tgtgctgatc gctcacttcc tgggtgtgga tcactgtggt cacaagcacg gccctcacca
      781 ccctgaaatg gccaagaaac ttagccagat ggaccaggtg atacagggac tcatagaacg
      841 cctggagaat gacacactgc tggtggtagc cggtgaccat gggatgacca tgaatgggga
      901 ccatggaggg gacagtgagc tggaggtgtc agctgcactt ttcctgtaca gccccacagc
      961 tctcttccct agtgtcccac ccgaggagcc ggaggttatt cctcaggtca gccttgtgcc
     1021 cacgctggca ctgctgctag gcctgcccat cccgtttggg aacactgggg aagtgatggc
     1081 agagctattc tcaggtggca gtgactcctc ccatcctcac tcctctgcgc tagcccaagt
     1141 ctcggctctc cacatcaatg cccagcaggt gtcccggttt cttcatacct actcggctgc
     1201 gactcaggat ctccaagtta aagaacttca tcggctgcag acgctcttct ccaaggcctc
     1261 tgcgcgttac cagcactttc tgcgggatcc ccaagaggct gaggcagcgc tgagcacatt
     1321 gactgctgag ttccagcagt tcctgcgggg agctcgggcc ctgtgcatcg agtcttgggc
     1381 ccgcttctct ctggtccgaa tggcaggggg cgctgctctt ttggctgctg cctgccttct
     1441 ctgcctgctt gcttcccagt tggcagtcgc cccaggcttt ctcttccgcc cactactgct
     1501 aatacctgta gcctggggcc taacttggac catcctgtat gctggagtct cggtaactac
     1561 tgggtcgaag atagacttag tggttctggg ggctgtggct gcagccggtt cactcctccc
     1621 ttttctgtgg aaagcttggg ttagcagggg gtctaagagg ccactggctc ccctacttcc
     1681 tgtccctaga cctgtcctaa tattactgct cattcgcttg gccaccttct tctcggatag
     1741 ctttgttgtt gctgaggcca gggccacccc cttccttctg ggctctctcg ttttcttcct
     1801 ggttgctcag ctccactggg agggtcagct actgccaccc aaaccactca cgatgtcccg
     1861 ccttggctct tctgccccaa cagcgccccc acggcacagt ggcgcgcatg ccctgtggct
     1921 tggaattggg ttgcttttat ttacaaggct cgctgggctt tttcaccgct gcccggaaga
     1981 gacacctgcc tgtcgctcct ctccctggct gagtcctctg gcatctatgg tgggaggtcg
     2041 agccaagaat ttgtggtacg gagcttgcgt gggggcactg gtggccctgt tagtcgtcgt
     2101 gcgcttgtgg cttcgccgct atggtaatct caagagtcct gagccccctg tgctctttgt
     2161 gcgctggggg atgcctctca tggtactggg cacggctgcc tactgggcac tggcatcagg
     2221 tgcagaggaa gcaccaccac gcctccgggc cctggtcgcc ggggcatcag ctgtgctgcc
     2281 tcgggctgtg atggggctgg ctgccttggg gctcgtgctg ctgctctgga ggcctgtgac
     2341 agtgctggtg aaagctgggg ctgctacttc aaggaccagg actatcctca ctcccttctc
     2401 aggtccccct acttctcagg ctgacctcga ctatgtagtc ccacaaatct accgccacat
     2461 gcaggaggag ttccagggcc ggctcgagag gactaaagct cagggtccca taaccgtggc
     2521 tgcctatcag ttgggaagcg tgtactcggc tgctatggtc acggccctcc tcctcttggc
     2581 cttcccactt atgctgttgc atgtagagcg ggtcagcctg gtgttcctgc tcctgtttct
     2641 gcagagcttc cttctcctgc atctgctggc cgctgggaca ccagtcgcca cccctggcca
     2701 ccagcctgtc ttttcagcca tccattggca cgcagccttt gtgggcttcc cagacggcca
     2761 tggctcttct acctggttgc ctgccttact agtgggagct aacacctttg cctcccacct
     2821 cctcttcgca gtaggctgcc cactgctcct gctctggccc ttcctgtgtg agcgtcaagg
     2881 gccaaagaga aggcagcccc tcccgggaag tgagtctgaa gccagagtcc ggcctgagga
     2941 agaggaggag ctgcaggagc cactgatgga gatgcgcctc cgggatgctc cgaatcactt
     3001 caatgcagcc ctgttgcagc tgggcctcaa gtacctcttt atccttggag ctcagattct
     3061 agcttgtgcc ttggcagctt ccatcctccg caggcacctc atggtctgga aggtgtttgc
     3121 tcccaagttc atttttgaag ccgtaggttt catcgtgagc agtgtgggac ttctcctggg
     3181 catagcattg gtgatgcgag tggatgtcgc tgtgagttcc tggttcaaaa aactagttct
     3241 ggcccagcag aggtagccca ggctgtgctc gttggcgtct ggccatggag agagctgggg
     3301 actaatccgg cctgacctgt actggtgctg gattctgcaa gagactccga cacacctctt
     3361 gagccaccct gtaaccagag actgctcaac tctgggactt tattatttta taactgagaa
     3421 tcatctggag cccgagcccg agcccgagcc ttccctcctg ggttggatac atctgacgga
     3481 caacaggggc ggctaccaaa gtggaataaa agaacaatgt gaatgtttta tgtagctaa
//
