LOCUS       NM_013784               6777 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            N (Pign), mRNA.
ACCESSION   NM_013784 XM_006529675 XR_106082 XR_107285
VERSION     NM_013784.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   McKean,D.M. and Niswander,L.
  TITLE     Defects in GPI biosynthesis perturb Cripto signaling during
            forebrain development in two new mouse models of holoprosencephaly
  JOURNAL   Biol Open 1 (9), 874-883 (2012)
   PUBMED   23213481
REFERENCE   3  (bases 1 to 6777)
  AUTHORS   Tamplin,O.J., Kinzel,D., Cox,B.J., Bell,C.E., Rossant,J. and
            Lickert,H.
  TITLE     Microarray analysis of Foxa2 mutant mouse embryos reveals novel
            gene expression and inductive roles for the gastrula organizer and
            its derivatives
  JOURNAL   BMC Genomics 9, 511 (2008)
   PUBMED   18973680
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6777)
  AUTHORS   Shishioh,N., Hong,Y., Ohishi,K., Ashida,H., Maeda,Y. and
            Kinoshita,T.
  TITLE     GPI7 is the second partner of PIG-F and involved in modification of
            glycosylphosphatidylinositol
  JOURNAL   J Biol Chem 280 (10), 9728-9734 (2005)
   PUBMED   15632136
REFERENCE   5  (bases 1 to 6777)
  AUTHORS   Hong,Y., Maeda,Y., Watanabe,R., Ohishi,K., Mishkind,M., Riezman,H.
            and Kinoshita,T.
  TITLE     Pig-n, a mammalian homologue of yeast Mcd4p, is involved in
            transferring phosphoethanolamine to the first mannose of the
            glycosylphosphatidylinositol
  JOURNAL   J Biol Chem 274 (49), 35099-35106 (1999)
   PUBMED   10574991
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166354.3 and AC132082.3.
            
            On or before Jul 22, 2015 this sequence version replaced
            XM_006529675.2, XR_107285.1, XR_106082.1, NM_013784.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660821.133193.1, AB030279.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-177               AC166354.3         92900-93076         c
            178-251             AC166354.3         88693-88766         c
            252-371             AC166354.3         88287-88406         c
            372-620             AC166354.3         87002-87250         c
            621-742             AC166354.3         86066-86187         c
            743-841             AC166354.3         85334-85432         c
            842-948             AC132082.3         163656-163762       c
            949-1073            AC132082.3         159835-159959       c
            1074-1204           AC132082.3         158661-158791       c
            1205-1321           AC132082.3         157250-157366       c
            1322-1362           AC132082.3         155031-155071       c
            1363-1422           AC132082.3         151217-151276       c
            1423-1515           AC132082.3         148735-148827       c
            1516-1571           AC132082.3         148062-148117       c
            1572-1650           AC132082.3         109601-109679       c
            1651-1833           AC132082.3         108220-108402       c
            1834-1973           AC132082.3         102174-102313       c
            1974-2073           AC132082.3         99778-99877         c
            2074-2166           AC132082.3         99601-99693         c
            2167-2258           AC132082.3         98532-98623         c
            2259-2367           AC132082.3         95541-95649         c
            2368-2476           AC132082.3         90474-90582         c
            2477-2579           AC132082.3         76700-76802         c
            2580-2682           AC132082.3         68540-68642         c
            2683-2769           AC132082.3         68015-68101         c
            2770-2825           AC132082.3         64996-65051         c
            2826-2901           AC132082.3         64616-64691         c
            2902-2975           AC132082.3         62924-62997         c
            2976-3018           AC132082.3         59617-59659         c
            3019-3071           AC132082.3         57216-57268         c
            3072-6777           AC132082.3         28974-32679         c
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 49.63 cM"
     gene            1..6777
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class N"
                     /db_xref="GeneID:27392"
                     /db_xref="MGI:MGI:1351629"
     exon            1..177
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            178..251
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            252..371
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            372..620
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     CDS             400..3195
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /note="phosphatidylinositol-glycan biosynthesis class N
                     protein; phosphatidylinositol glycan, class N"
                     /codon_start=1
                     /product="GPI ethanolamine phosphate transferase 1"
                     /protein_id="NP_038812.2"
                     /db_xref="CCDS:CCDS15205.1"
                     /db_xref="GeneID:27392"
                     /db_xref="MGI:MGI:1351629"
                     /translation="MLLFFALGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPAK
                     RLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSWGVSHTRVPTESRPGHVALI
                     AGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPDILPMFAKGASGDHVYTYSYD
                     AQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFFLHLLGIDTNGH
                     AHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTDWGSHGAGHP
                     SETLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPLMASLIGV
                     PFPLNSVGILPVGYLNNTGLFKAESMFTNAVQILEQFKVKMTQKKEATLPFLFTPFKL
                     LSDSQQLDILRKARSYIKQEKFDEVVSLCEELIDLALRGLSYYHTYDRLFLGINVAVG
                     FVGWMSYTSLLIIKSHSNIPKGTRKEGKKPHCLLLYSFIATGVLVACFLMIQACPWTY
                     YVYCLLPVPIWYAVLREHEVIQDLVESLLTFPRSHFVAYLLVFTLGIEVLVLSFFYRY
                     MLTAGLIVFAGWPFLTQLWTRAKITFLSWAFFSLLLAVFPLMPVVGRKPNLSLVMGAG
                     FLVLLLSLAVVTTLGKRNIKLVKGELLVLLLQMLSTVLSMYVVYSTHHSLLKKEGLPL
                     MNQIVSWATLASSLVAPLLSSTALSQRLASILLSLMSTYLLLSTGYEALFPLVLSCLM
                     FVWIQVEQETLQQPGVSCKQKLTSIQFTCDTDIAQFRQLCPDDIRRAFFLVFFLLTAF
                     FGTGNIASINSFDLASVYCFLTVFSPFMMGALMMWKILIPFVLVMCAFEAVQITTQLS
                     SKGLFLVVLIISDIMALHFFFLVKDSGSWLDIGTSISHYVIVMSMTIFLVFLNGLAQL
                     LTTKKLQLCGKPKSHLM"
     exon            621..742
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            743..841
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            842..948
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1073
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1204
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1321
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1362
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1422
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1515
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1571
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1650
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1833
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1973
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1974..2073
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2166
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2258
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2367
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2476
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2579
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2580..2682
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2683..2769
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2770..2825
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2826..2901
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2902..2975
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2976..3018
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            3019..3071
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            3072..6777
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6244..6249
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /note="hexamer: AATAAA"
     polyA_site      6264
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /note="major polyA site"
     regulatory      6378..6383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /note="hexamer: AATAAA"
     regulatory      6389..6394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /note="hexamer: AATAAA"
     polyA_site      6406
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /note="major polyA site"
     polyA_site      6410
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /note="major polyA site"
     regulatory      6754..6759
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /note="hexamer: AATAAA"
     polyA_site      6773
                     /gene="Pign"
                     /gene_synonym="Gm20308; PIG-N"
                     /note="major polyA site"
ORIGIN      
        1 ggcaggttgg gggcgttccc tggagcagta gaggtggagg tcgctgaggg gtacgatttg
       61 gctgtggttt ttgagggttg aagggaaccc tcccgacatc tttcaccgcc ctgggtcctg
      121 ccttcgttct gaggaggtcc cgttcccaag actgggcctg agccggatag ttctcagtga
      181 ctcctgtaaa aaggtcgtct taccccacaa agaaatcatg acccacaagt tgagaactgc
      241 tgacttagaa ggacattctc catccttatg atagttaatt cttcagacca ggaagaataa
      301 gatatgtcca taattcttat ctttcatatt ttcaagatca aggtcattat ttgtatcaaa
      361 tactgcttca acttctcttg caggtagccc agtgtggata tgctgctgtt ttttgctttg
      421 ggattgctta tccattttgt gttcttcgct tccatctttg acatttattt tacatctcct
      481 ttggttcatg gaatgactcc ccagtttaca cctttgcctc ctccagcaaa aagattagtg
      541 ctatttgttg ctgatggcct gagagcagat actctttatg aattagatga agatggaaac
      601 tccagagcac ctttcattag gaatgttata atgcatgaag gcagctgggg agtatctcac
      661 acacgtgtgc caacagaatc tcggcctggc catgtagcct tgatagctgg attttatgag
      721 gatgtcagtg cagttgccaa aggatggaaa gaaaatcctg tagaatttga ctctcttttt
      781 aatgaaagca agtacacgtg gagctggggg agtccagata tcttgcccat gtttgccaaa
      841 ggtgctagtg gtgaccatgt ctatacatat agttacgatg ctcaaagaga agattttggt
      901 gcccacgatg ccacaaaact ggatacgtgg gtttttgata aagttaagga cttctttgat
      961 gctgcaagaa acaaccaatc tttgttcaca aaagtaaatg aagaaaaagt agtttttttc
     1021 ttacatttat tgggaataga tacaaatgga catgctcatc gaccatcgtc aagggaatat
     1081 aaggacaata ttaaaaaagt tgatgatgga gtgaaggaaa tcgtatcaat tttcaagcat
     1141 ttttatggag atgatgggaa aacagcattt atctttacct ctgaccatgg aatgacagac
     1201 tggggttccc atggggctgg tcatccgtca gagactttaa ctccgttcgt tacttgggga
     1261 gctggaatca aatttcctca aaatgtatca gctcagcaat atgatgatga gtttttaaaa
     1321 gaatggagat tggaaaactg gaaaaggaga gatgtcaatc aggctgatat tgcacctctg
     1381 atggcatccc ttattggagt cccctttcct cttaattcgg tgggaatact tcctgtgggt
     1441 tatcttaaca acactggtct cttcaaagca gagagcatgt ttaccaatgc agtacagatt
     1501 cttgaacagt tcaaggtgaa aatgactcag aaaaaagaag ccactttacc gtttttgttt
     1561 acaccattta agttgctttc tgattctcaa caactggaca ttttaagaaa agcaagatct
     1621 tacataaaac aagaaaaatt tgatgaagta gtgtccctct gtgaggagct gattgacctc
     1681 gccctgagag gattgtccta ttatcatact tatgacagac tgtttctggg catcaatgtt
     1741 gcagttggtt ttgtgggatg gatgtcttat acttctttgt taatcatcaa gtcgcattct
     1801 aatatcccaa aaggcactag gaaggaaggt aagaagccac actgtctcct gctttatagc
     1861 ttcatagcta ctggtgtctt agtagcatgc ttcctgatga tccaagcctg tccctggact
     1921 tactatgtgt actgcttgct gccagtgcct atatggtatg cagttctaag agaacatgaa
     1981 gttattcaag acctggttga atcactattg acttttcctc ggagccattt tgttgcctac
     2041 ttgcttgtct ttaccttggg tattgaagta ctggtgctca gtttcttcta ccgctacatg
     2101 ttgacagcgg gactgattgt gtttgcaggc tggccgtttc tcactcagct gtggacccga
     2161 gcaaagatca ccttcctgag ttgggctttc ttctctttgc ttctggcagt gttcccactg
     2221 atgcctgtcg tgggtagaaa gccaaacctt tctctagtaa tgggtgctgg attcctggtt
     2281 cttctgctgt cgctggctgt tgtaacaact ctcggaaaaa gaaacattaa attggtaaag
     2341 ggcgagctgt tggtacttct gttacagatg ctgagtacag tgctctctat gtatgtggtg
     2401 tatagcaccc accacagtct gttaaagaaa gaaggcctgc ctcttatgaa tcagattgtg
     2461 agctgggcaa cattagcctc ttctctggtg gcaccactcc tgagctccac agctctctct
     2521 cagcgactgg ccagcatact cctctccttg atgtccacct acctgctcct aagcactggg
     2581 tatgaagctc tctttccttt agtgctgtcc tgtttgatgt ttgtctggat ccaagtggaa
     2641 caagagaccc ttcaacagcc tggtgtttct tgtaaacaga agctcaccag catccagttc
     2701 acctgtgaca ctgatatagc tcagttccga cagctctgtc cggatgacat ccgtagggcc
     2761 tttttcctag ttttcttttt attaactgca ttttttggaa ctggaaatat agcttctatt
     2821 aacagcttcg accttgcctc tgtgtattgc tttctgactg tgtttagtcc tttcatgatg
     2881 ggagcgctca tgatgtggaa gattttaatt ccttttgttc ttgtgatgtg tgcttttgaa
     2941 gcagttcaga taactacaca gctgtcctca aaaggtcttt ttcttgttgt tctcatcata
     3001 tcagacatca tggctttgca ttttttcttc ctggtcaagg attctggcag ctggcttgat
     3061 attggaacaa gcatcagcca ctatgtgatt gtcatgtcca tgaccatctt cctggtattt
     3121 cttaatggcc tggcacaatt gctcacaaca aagaaactcc aactgtgtgg caaacccaaa
     3181 agtcatctta tgtgatgttg gtgaagccat cagtcagcat gcatcctgat tctccttctt
     3241 aaagaaaagt cccactaagg attcaagaaa atggatattg gaccagcaaa atggctcagt
     3301 gggtaaaagt tcttgttgca caagctgaca acctgagttt gatccctgga acccacactg
     3361 gaaggagaaa atagacttcc aaaggttgtc ctttagcccc caagtacatg ctgtgacaca
     3421 cacacacaca cattgcactt gcacatatgt catgcatgta tacatcacat gcatacatga
     3481 tgacactgca gttttaagag atgatggata ataatgcaca gtacattctc atctagagaa
     3541 tgttgcatct ggcattctgg atttaaaggt gttctgaaat aatagttttt cttttgtctg
     3601 acagcttttc ccagtaatgt ttgagagcac ctgcagccct ttgctcctca ctgtcaactt
     3661 catgtacctg tgggggtggt actgacactc tacaaagtca ttcccaagtg gtgactgaat
     3721 ggaaccacag gttacaacat aaacccagca ttgacttgct tttgtggtag ctctgacttc
     3781 tagaagggct gcaatctggg atttactgag cctctgagca aagctgtctg ccaggctttg
     3841 ccatttctgc tcctgcagta cgttggcttg tgcctctctg tgccacttcc gggattctca
     3901 tagtcagcca attagcaaag ctttcacctt tacattttgt caacttctgg atcatcttca
     3961 aattttggga gactctgtgg ctttgttgat tattgtttca gtgtcagtcc ccaaatcagt
     4021 ggctgtagtt tcctaacagt atcataaaga atcaaataaa cttgctactc ttttgtcttg
     4081 atattctagc tagaccaaag gccagccatc ttaatagagt gagcttttcc tttatatatt
     4141 ctaatatatg aggtaatttg agagtaattt ttatttgcgt tttccataaa gtgacctata
     4201 cagtgtagag ttacctgtac aatactgtta agtgcttggc tgatattttg ctggtatata
     4261 tcccatttta aaaaacagtt gcttttttga ttcaagttat attactagtc tacaatgatg
     4321 accaaaaagc tctttacaag tttttaaact tttcaaattg gcaggtaagg aaatatgtat
     4381 ctagaagagt aagctcagct gagcaaaata tactttattt tctactgata tgtcaggaga
     4441 cccattatag tgaattaagc gagaagtact aatcctagca cgggcatcac tgtgggacag
     4501 tccaggtggc agctatgctg acacaggcag aacagggccc agctagtttc ctagaccctc
     4561 aggtgacttc tgaccccatt atatgaacac ttaccacatt cctcatctgc aggggttaat
     4621 tttaagtaag tgtgtgtgtg tgtgtgtgtg tgtgttcatg cacatgagca caggtgccct
     4681 cagagtccat aagagggtgt tagatccctg aagctatagt tacaaattgt gagttgtaag
     4741 atacctaaca tgggtactga gaaccaaact tgggtcctct gcaagaataa tatttacttt
     4801 ttaagtactg agacatctct ccaattccag aattaatgtt tttaaactaa cagagtaagt
     4861 ggtgctttct gccttgtatt ttgcattgta ttctccaaag tacattctta tgtctgggct
     4921 gcttccagtc tgtagaaggc actgcttctg caattgcagc actccctgca gcactgacac
     4981 tcacattcac tgggggaggg agcactcttc cctctggcac agtgtttctc atatcttgaa
     5041 atgtatattt ttgtactgaa tagcagaagt aaatgagatt taggcaacat ttttatttct
     5101 ctttaaggta ttttcttcct tttgttaata ttatactagg tttcaaaaca aaaacttaca
     5161 aaatgtgcca gtcagcactg tttaaattag taagtgcttt agaaagtagg gataaactgg
     5221 tatgatggca gatatctgta atcccagcac tagggaggtg taggcaggag atcatgagtt
     5281 gaaggtcata gaaaattaga caccagcctg tgcttctaaa gccctatgtt ttctaaaaag
     5341 aattaaagta ggtatctcaa ggtgaaatta attaaaatag tgaatgacag ctgaatcatg
     5401 aatgaatctt tgtttactga ctatataatg gtcataattt gataagttct ttgtatttta
     5461 ttatttagtg tataaagtct tagaacacaa aatgctttct tacattttag aaaaaaatgt
     5521 attacaagct gatgtggtga cacaggcctg actataatcc caaagtttac aaggctgagg
     5581 aggaggatca catgttcagg gtcagcataa acaccacatg ttacgtgaga ccctgtatta
     5641 ggaaacaatg aggtagggga ctgttgtatt ataagatggc tgtatgtgaa gaatgatgta
     5701 acctaattat cagggaactg aacttgaaag tgactcataa gttagtgtgg cttctcatga
     5761 aaacctcatt ccagatttca tgctacttac acatcagtcc tcctccctgc taccgcctgc
     5821 acctttacat actgttagca atcgagggtt cctttgttct tccttttgaa actagattct
     5881 aatgctagta gttcaggggg tagtgaagtg ctacacagtc atgcctttag agacaggcag
     5941 cagctaagtg ttcccctctc cttgtgggag ccaagcgagt tcattttccc attagatgcc
     6001 atctcatcag gaagctagaa tgacactgaa acaactcagt aatggagagt ttccatgtcc
     6061 cactagtact caggagcctt taagagacca aaggtagcct gatcaggggc tctcctttcc
     6121 tcaatagtgc ttaagattat tgtgctgtgt gctttctgtc cctggtaatg tcttggcagc
     6181 atttcccatt tcctccctgg tatagtttgt aaaaatagat ttctcaatgt aattctgtta
     6241 agaaataaaa atgtttgact tgtatatgtc actaattttc tctagtacac taaaatagat
     6301 tgttaatgga ctcaccattg gaattgtaat atttatgtgc agttttgaaa attgtttcaa
     6361 agcatcactt gttgatgaat aaatatataa taaaagggtt ctctgatgaa gtgtttagga
     6421 aacatctagg ttagaaggtt taaaggtgca tttatttctg gtagttatat gaatttattc
     6481 aggatgccca caacttccat atgtctggca gcagagtgtt tcctccctct tggtgggaga
     6541 catatatttt acaaaagttt ttatgtactt ttctaaaaaa tttttacaaa caagtgttgt
     6601 gcatttaatt gaagcttttc ttggcaactt gtaaagtgtg ttcattctaa ctgttaaact
     6661 gagaaaactt gagaacatac tggggatttt gcctgcacat ccctgtctgt gcagtatgga
     6721 gtttcatctc ttttgcatcc tcaacctgaa aaaaataaaa tcacaactct caactgg
//
