LOCUS       NM_001039536            3813 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            L (Pigl), transcript variant 1, mRNA.
ACCESSION   NM_001039536
VERSION     NM_001039536.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3813)
  AUTHORS   Perez-Garcia,V., Fineberg,E., Wilson,R., Murray,A., Mazzeo,C.I.,
            Tudor,C., Sienerth,A., White,J.K., Tuck,E., Ryder,E.J., Gleeson,D.,
            Siragher,E., Wardle-Jones,H., Staudt,N., Wali,N., Collins,J.,
            Geyer,S., Busch-Nentwich,E.M., Galli,A., Smith,J.C., Robertson,E.,
            Adams,D.J., Weninger,W.J., Mohun,T. and Hemberger,M.
  TITLE     Placentation defects are highly prevalent in embryonic lethal mouse
            mutants
  JOURNAL   Nature 555 (7697), 463-468 (2018)
   PUBMED   29539633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX248411.3, BX470084.10 and AL596181.10.
            
            On Jun 21, 2019 this sequence version replaced NM_001039536.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK158149.1, SRR7652917.1194428.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-254               BX248411.3         71952-72205
            255-354             BX470084.10        14090-14189
            355-445             AL596181.10        170382-170472       c
            446-513             AL596181.10        166357-166424       c
            514-545             AL596181.10        164300-164331       c
            546-679             AL596181.10        161880-162013       c
            680-3813            AL596181.10        158114-161247       c
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 38.34 cM"
     gene            1..3813
                     /gene="Pigl"
                     /gene_synonym="Gm737"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class L"
                     /db_xref="GeneID:327942"
                     /db_xref="MGI:MGI:2681271"
     exon            1..254
                     /gene="Pigl"
                     /gene_synonym="Gm737"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..778
                     /gene="Pigl"
                     /gene_synonym="Gm737"
                     /EC_number="3.5.1.89"
                     /note="isoform 1 is encoded by transcript variant 1;
                     N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase;
                     ortholog of human and rat phosphatidylinositol glycan
                     class L PIGL; phosphatidylinositol-glycan biosynthesis
                     class L protein"
                     /codon_start=1
                     /product="N-acetylglucosaminyl-phosphatidylinositol
                     de-N-acetylase isoform 1"
                     /protein_id="NP_001034625.1"
                     /db_xref="CCDS:CCDS24824.1"
                     /db_xref="GeneID:327942"
                     /db_xref="MGI:MGI:2681271"
                     /translation="MELVGFLCVAVAVLTWGFLRVWNSAERMRSPEQAGLPGAGSRAL
                     VVIAHPDDEAMFFAPTMLGLARLEQQVSLLCFSSGNYYNQGEIRKKELLQSCAVLGIP
                     PSRVMIIDKRDFPDDPEVQWDTELVASTLLQHIHANGTDLVVTFDAEGVSGHSNHIAL
                     YKAVRALHSGGKLPKGCSVLTLQSVNALRKYAFLLDLPWTLLSPQDVLFVLTSKEVAQ
                     AKKAMSCHRSQLLWFRYLYVLFSRYMRINSLRFL"
     exon            255..354
                     /gene="Pigl"
                     /gene_synonym="Gm737"
                     /inference="alignment:Splign:2.1.0"
     exon            355..445
                     /gene="Pigl"
                     /gene_synonym="Gm737"
                     /inference="alignment:Splign:2.1.0"
     exon            446..513
                     /gene="Pigl"
                     /gene_synonym="Gm737"
                     /inference="alignment:Splign:2.1.0"
     exon            514..545
                     /gene="Pigl"
                     /gene_synonym="Gm737"
                     /inference="alignment:Splign:2.1.0"
     exon            546..679
                     /gene="Pigl"
                     /gene_synonym="Gm737"
                     /inference="alignment:Splign:2.1.0"
     exon            680..3813
                     /gene="Pigl"
                     /gene_synonym="Gm737"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1647..1652
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigl"
                     /gene_synonym="Gm737"
                     /note="hexamer: AATAAA"
     polyA_site      1680
                     /gene="Pigl"
                     /gene_synonym="Gm737"
                     /note="major polyA site"
ORIGIN      
        1 gcagtgccac ttacccggca tggaattggt gggtttcttg tgtgtggccg tggctgtcct
       61 gacttggggc ttcctccggg tttggaactc agcggaacga atgaggtctc cggagcaggc
      121 gggcctgccg ggagccggaa gccgggccct tgtagtgatc gcgcacccgg acgatgaagc
      181 catgttcttt gctcccacca tgctaggctt ggcacgcctg gagcagcagg tgtccctgct
      241 ctgcttctcc tcaggaaact actacaatca aggtgagatt cgaaagaaag agcttctgca
      301 aagctgtgct gtgttgggga ttccaccttc ccgtgtaatg attattgaca agagggactt
      361 tccggatgat ccagaagtgc agtgggacac ggagctcgtg gccagcaccc tccttcagca
      421 catacatgcc aatggcactg acctggtggt gacttttgat gcagaaggag tcagtggtca
      481 cagcaaccat attgctttgt acaaagctgt gagggccctg cactcaggag ggaagctgcc
      541 taaagggtgc tccgtgctca cgctccagtc tgtgaacgcg cttcggaagt acgccttcct
      601 cctggatctg ccctggactc tgctgtcacc ccaggatgtt ctctttgtgc tcaccagcaa
      661 agaagtggca caggccaaga aagccatgtc ctgccaccgc agccagctcc tctggttccg
      721 atacctctac gtcctcttct ccagatacat gagaatcaac tcactgcggt tcctctgaag
      781 ccttggagag tggtcagacc ctcagaacaa gagggagaag cagagcaaga agtcgtctgg
      841 ccaggctttg ggctgattta cctgcctgac ccagggaaac cccagcaaag cagcgtctgc
      901 caaacaggga ccacatgaag gatgggtcac cccaacccag cttcctcttc tgaggaaaac
      961 ctgccaaaac caagcctaca ccaacacaat agtagccacc ccactaattt accaatagtt
     1021 tgcatgataa cagagtttgc accagacatc cagcaaagcc tagggaagtt gtctttacca
     1081 caaatgtgtg taaatgtgcc atagaactag ttcacaggtg tgatgtttac atggtttcca
     1141 gaccaggctg ttttggtcag tcttctgttc ctgtagctga aacaccgctc acagggcatc
     1201 tgcagagctg cggttttctt ggatcacagt tctgaagcct gtgaagtcca agagcatagc
     1261 agcctctaca tgaccgcggt gaggggcatc ccgctgcatc tcaaaggcaa aggcagtcag
     1321 tcagaaagag cccatgagcc aaagagttcc tttagaacaa agccacgcct acaacaaata
     1381 accctgtcac ttccaaaagg cctcacccta aacactgggg ctctgaagac acatgaattc
     1441 caggggacac agccaaaccc tagcacaggt tcatccagac acccctctcc cctcccccca
     1501 cagtcttcag gtgcttcaaa ctattccttg ggtttttatt tgtgtcccct tatgagaact
     1561 ttacagtcta gagaagctgt accttatcat catggtggtt ctgctgtgtg tggagatgat
     1621 cctgtgggtc ccccctttaa ggagccaata aacaccttgg ttctctgcct tttcacagta
     1681 ggagctgggc ccacaccttt atatatatac tttttttttt tttttaagat ttatttgtta
     1741 tatgtaagta cactgtagct atcttcagac actccagaag agggcatcag atctcattac
     1801 agatggttgt gagccaccat gtggttgctg ggatttgaac tcaggacctt ctgaagagca
     1861 gtcggcgctc ttaaccactg agccatctcg ccagcccata tatacttttt ttaaaaagaa
     1921 aaagaaagaa atggaaagaa aatacaaaca ttattctgat agtgaaatat tgaaccagta
     1981 caaaataaat aaaatatggg aagctgggtg tagtagtata tgcctttaat cccagcattc
     2041 agaagacaga gacttgtgga tatctctaag tttgtggcca gcctggtcaa catagtgagt
     2101 tccaggacat ccagagctac ataatgagtt tctttaaaaa gtaaaaaaat aacctcaaat
     2161 agttattcta ataggatgga tagtgataaa accacgttta cataaagcag tatacccaca
     2221 tgtaaaaatc tacatgataa tgaacaaata gtgttcttag cctgctttgt ttgggggaag
     2281 aagtgtattt ccatagccac aggtgtaagc aagcagagct ttgcccatgc ctgtggagcc
     2341 tgtctctgaa gagtcctttt gtactgtgtt cctgcactga gccctctgca aggccgattc
     2401 gctgattagc aacgacacag aagttgaaag gctgcatgtc cagaaccaag ttatattggg
     2461 gtgtccttag acaccttgca aactgtctgc tccccacttc tgtatctgac ctagcatctt
     2521 tgtgaccgtt gggtaaacac ttgggaatat agaagcagct gcctagttcc caccaaccaa
     2581 aggctaggga ttctttcaga tagcatctga ggcgtccagc tgagatgcct tccttccctg
     2641 tgatccacct acctgatgtt cccactgtgt gtgtgagaag tgcccatgtg tgactgtgtg
     2701 tgtgagaagt gcccatgtgt gactgtgtgt gtgagaagtg cccatgtgtg actgtgtgtg
     2761 tgagaagtgc ccatgtgtga ctgtgtgtgt gagaagtgcc catgtgtgac tgtgtgtgtg
     2821 agaagtgccc atgtgtgact gtgtgtgtga gaagtgccca tgtgtgactg tgtgtgtgag
     2881 aagtgcccat gtgtgactgt gtgtgtgaga agtgcccatg tgtgactgtg tgtgtgagaa
     2941 gtgcccatgt gtgactgtgt gtgtgagaag tgcccatgtg tgactgtgtg tgtgagaagt
     3001 gcccatgtgt gactgtgtgt gtgagaagtg cccatgtgtg actgtgtgtg tgagaagtgc
     3061 ccatgtgtga ctgtgtgtgt gagaagtgcc catgtgtgac tgtgtgtgtg agaagtgccc
     3121 atgtgtgact gtgtgtgtga gaagtgccca tgtgtgactg tgtgtgtgag aagtgcccat
     3181 gtgtgactgt gtgtgtgaga agtgcccatg tgtgactgtg tgtgtgagaa gtgcccatgt
     3241 gtgactgtgt gtgtgagaag tgcccatgtg tgactgtgtg tgtgagaagt gcccatgtgt
     3301 gactgtgtgt gtgagaagtg cccatgtgtg actgtgtgtg tgagaagtgc ccatgtgtga
     3361 ctgtgtgtgt gagaagtgcc catgtgtgac tgtgtgtgtg tgagaagtgc ccatgtgtga
     3421 ctgtgtgtga gaagagccca tgtatgattg tgtgtgtgtg tgagaagagc ccctgagtga
     3481 ctatgtgtgt gagaagtgcc cacgtgtgag aagaacccat gtatgactgt tgtgagagaa
     3541 gtgcccgtgt acgatttaag tagggcttct gctataaaac ttcatggaac tgtttccaca
     3601 ttgggacatg gaactggggt gacctgaatg tgtgtcctgg gacaccagca catattgcat
     3661 cccaggataa actacctcac atctctttga agtgagaact gttattttgt tgacaatacc
     3721 ccttgagggt ttttttttta tgatatatac acagtacaac ctatgaaaaa tgttttcaaa
     3781 tctgagacat taaaaagaca catagaagaa cta
//
