LOCUS       NM_178016               4885 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            K (Pigk), transcript variant 2, mRNA.
ACCESSION   NM_178016
VERSION     NM_178016.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Chen,X., Yin,W., Chen,S., Zhang,W., Li,H., Kuang,H., Zhou,M.,
            Teng,Y., Zhang,J., Shen,G., Liang,D., Li,Z., Hu,B. and Wu,L.
  TITLE     Loss of PIGK function causes severe infantile encephalopathy and
            extensive neuronal apoptosis
  JOURNAL   Hum Genet 140 (5), 791-803 (2021)
   PUBMED   33392778
REFERENCE   3  (bases 1 to 4885)
  AUTHORS   Boutlis,C.S., Riley,E.M., Anstey,N.M. and de Souza,J.B.
  TITLE     Glycosylphosphatidylinositols in malaria pathogenesis and immunity:
            potential for therapeutic inhibition and vaccination
  JOURNAL   Curr Top Microbiol Immunol 297, 145-185 (2005)
   PUBMED   16265905
  REMARK    Review article
REFERENCE   4  (bases 1 to 4885)
  AUTHORS   Eisenhaber,B., Maurer-Stroh,S., Novatchkova,M., Schneider,G. and
            Eisenhaber,F.
  TITLE     Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and
            post-translational transfer to proteins
  JOURNAL   Bioessays 25 (4), 367-385 (2003)
   PUBMED   12655644
  REMARK    Review article
REFERENCE   5  (bases 1 to 4885)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   6  (bases 1 to 4885)
  AUTHORS   Yu,J., Nagarajan,S., Knez,J.J., Udenfriend,S., Chen,R. and
            Medof,M.E.
  TITLE     The affected gene underlying the class K
            glycosylphosphatidylinositol (GPI) surface protein defect codes for
            the GPI transamidase
  JOURNAL   Proc Natl Acad Sci U S A 94 (23), 12580-12585 (1997)
   PUBMED   9356492
  REMARK    GeneRIF: The protein encoded by the human ortholog catalyzes the
            final step in glycosylphosphatidylinositol (GPI) anchoring of cell
            surface proteins, the transamidation reaction.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK077893.1, AK046246.1 and BC060175.1.
            
            On Jun 19, 2009 this sequence version replaced NM_178016.2.
            
            Transcript Variant: This variant (2) represents the longer
            transcript and encodes the longer isoform (2).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK046246.1, ERR3363658.970198.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164132, SAMN02415123
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AK077893.1         2-14
            14-1774             AK046246.1         2-1762
            1775-4885           BC060175.1         1620-4730
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3"
     gene            1..4885
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class K"
                     /db_xref="GeneID:329777"
                     /db_xref="MGI:MGI:1913863"
     exon            1..128
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..1364
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /EC_number="3.-.-.-"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; GPI transamidase; GPI-anchor transamidase;
                     phosphatidylinositol-glycan biosynthesis class K protein"
                     /codon_start=1
                     /product="GPI-anchor transamidase isoform 2 precursor"
                     /protein_id="NP_821135.1"
                     /db_xref="CCDS:CCDS17920.1"
                     /db_xref="GeneID:329777"
                     /db_xref="MGI:MGI:1913863"
                     /translation="MAAPCFLTLRVATLAALALLSLGSSAAGHIEDQAEQFFRSGHTN
                     NWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNARNPKPATV
                     FSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRVPPSTPRSKRLLSDDRSNILIY
                     MTESAPATPALAFVPLGSSFSAHNRTADLPFSAAHSQILLLKDLLSNSLSHGGNGFLK
                     FQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSPNIMALASSQVG
                     EDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPASQTNMNDLFQVCPKSLCVSTPGHR
                     TDLFQRDPKNVLITDFFGSVRKVEITTEKISLQWDSQVVDSSSKEDGTAEERMGPLKY
                     AEQLPVAQIIHQKPKPRDWHPPGGFILGLWALIIMVFFKTYGIKHMKFIF"
     exon            129..182
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            183..274
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            275..410
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            411..522
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            523..663
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            664..760
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            761..878
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            879..989
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1162
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1247
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..4867
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1659..1664
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /note="hexamer: AGTAAA"
     polyA_site      1703
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /note="major polyA site"
     polyA_site      1777
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
     polyA_site      3303
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
     polyA_site      4745
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
ORIGIN      
        1 ccctttgctc ttcggggcgt gagaagccgg caaacatggc ggccccctgc ttcctcactc
       61 tgcgggtggc taccctggcc gccttggcgc tcttgtctct cggcagctct gccgctggac
      121 acatcgagga tcaagccgaa cagttcttta gaagtggaca cacaaataac tgggctgttt
      181 tggtgtgcac atcccgattc tggtttaact accgacatgt tgcaaatact ctttctgttt
      241 atagaagcgt caagaggcta ggtatcccgg acagtcacat tgtcctgatg cttgctgatg
      301 acatggcgtg caatgctcgg aaccccaagc cagccacagt gttcagccac aagaacatgg
      361 agctcaatgt gtatggagac gatgtggaag tggactacag aagctatgag gtaactgtgg
      421 agaacttttt aagggtattg accgggaggg ttccacccag tacccctcgc tcaaagcgtc
      481 ttctttccga cgacagaagc aatatcctca tttatatgac agagtccgct cctgccacac
      541 ccgctctggc ttttgtcccc cttgggtcgt ccttctcagc acataataga actgcagatc
      601 tgcccttctc tgccgcacac tcccaaattc tacttttgaa ggatttactc agtaactcct
      661 tgagtcatgg agggaatggt ttcttgaaat tccaagattc tgaagaaatc accaacatag
      721 aacttgcaga tgcgtttgaa cagatgtggc agaagagacg ctacaatgag ctgctgttca
      781 ttattgacac ttgccagggc gcgtccatgt acgagcggtt ctactctcct aacatcatgg
      841 ccttggctag tagccaggtg ggagaggatt cgctgtcgca ccagcctgac cctgcgattg
      901 gagttcatct tatggatagg tacacgtttt atgtcttgga atttttggaa gaaattaatc
      961 cagctagcca aactaacatg aacgaccttt ttcaagtgtg tcccaaaagt ctctgtgtgt
     1021 cgacccctgg acatcgcact gaccttttcc agcgagatcc aaaaaatgtc ctgatcaccg
     1081 atttcttcgg aagtgtgcgc aaggtggaaa tcacaacaga gaagatcagt ttgcagtggg
     1141 attcacaagt cgtggacagc agttctaaag aagacggcac ggccgaggag cgcatgggac
     1201 ctctcaagta tgctgagcag ctcccggtgg ctcagataat acaccagaag ccaaagccga
     1261 gagactggca ccctcccgga ggcttcatcc tggggctgtg ggcgctcatc atcatggtct
     1321 tcttcaagac ctatgggatc aagcatatga agttcatttt ctaagactca aggatgtatg
     1381 aggaagaatg aatggaagac tgcagccttg gagcaaaact taggttttca tatgttttta
     1441 aatatgttgc ttttgtgtaa atttgggaag aaaattagga aaattgaata ttaatgaact
     1501 tttgacttta aaacataata ttgttaatgc acttgtttac tggccacaga atatatcttt
     1561 cattgtgttt gtgtgtttga gttatagaaa gcagagagca atgacattgg aactttatgc
     1621 ttaattcttt ttaccctcct ctcattgtaa aagtgagtag taaaggtgtt agttcccact
     1681 aaaggaaatt acttccgttc ataggtttgt gtgtgctgtc ttggtctgtt tatctatgga
     1741 catatgttcc caaccataca gaatatgcca tctctaatct ttggtgttta caatgcatta
     1801 ttaacctttc tccgccctcg gacatccttc aggatatact gtgagcctga cttggatgca
     1861 cttgcctaca gtccaggctg ttcaggaggc cgagtcgatt tttgagccct gggggtgttg
     1921 aaggtcagcc tgggcaacaa acaacaacca acagaagaga gaaacaccac ggaatgcacc
     1981 gcccactcta tggtggtcac gtgacagctc ctgagggacc cttacctgtt cctagagttc
     2041 ccttgggctc actctgggat agtaatgcct ctggaattct gcagctttac tcgcacagga
     2101 agatggagac acagctttac tcgcacagga agatggagac acagctttac tcgcacagga
     2161 agatggagac acagctttac tcgcacagga agatggagac acagctttac tcgcacagga
     2221 agatggagac acagctttaa ttgcacagga agatggaaac acagctttac tcgcacagga
     2281 agatggagac acagctttac tcgcacagga agatggagac acagctttac tcgcacagga
     2341 agatggagac acagctttac tcgcacagga agatggagac acagctttac tcgcacagga
     2401 agatggagac acagctttac tcgcacagga agatggagac acagctttac tcgcacagga
     2461 agatggagac acagctttac tcgcacagga agatggagac acagctttac tcgcacagga
     2521 agatggagac acagctttac tcgcacagga agatggagac acagctttac tcgcacagga
     2581 agatggagac acagctttac tcgcacagga agatggagac acagctttac tcgcacagga
     2641 agatggagac acagctttac tcgcacagga agatggagac acagctttac tcgcacagga
     2701 agatggagac acagctttac tcgcacagga agatggagac acagctttac tcgcacagga
     2761 agatggagac acagctttac tcgcacagga agatggagac acagctttaa tcgcacagga
     2821 agatggagac acagctttgg tagctgttta tctagggaag tcatgcctct gagaattttt
     2881 agtggtaacc cctcagagaa acatgcccta ttacttttta tttttaagca tactgtgaac
     2941 ttctagaatt gattgtatct cttcagtttt tgtcctgaaa gagacagatt gctgacagct
     3001 ggatctatgc tgtccctgag gacagcccca taaactggat gacatacatg gagcacacac
     3061 actgaaggcc ccgccttcat gtctgatgga tcagtaagac acatccaata ctgactcttc
     3121 taggatgtca gtcacctcca catcttcctg cggtgaaaag atggccaagc ttgtgttttg
     3181 tgaccattct cagacactct tttctctttc agactttggc atcagagagc cgtgctgggg
     3241 tgggctgtgc ctatagattt actattcatg tctgaggaga atcttaggtg tttcatccaa
     3301 gaatctatat gttcatctgt tttctttaca aatactgttt tctaattgta ggtaacaatt
     3361 aagaagtctt ctaatcccct ggcaataggc agatacacct tgtttgtgat attgctcttt
     3421 tgtcagtcag gggcagagca catggcagtc cttgttcatc ctagcttgtt gcctgccctg
     3481 gtggccaaag ttgtgtcatg cctaggacac ataaaaatca aatcatcaaa tcaaattgag
     3541 gcaccttggg aggaagacag tagacagagg cctgtggacc atggaccacg tgccacccac
     3601 agacctgctc tctgatgtat gacacaggtg ggctgtatat acccacccac tgtgcataga
     3661 caggatttca tccaggggcc caccagcttc atggcagcct gctctgcagg agtcaccacg
     3721 atgtgttagt tagtatgtgc tagtcaggtg ggagactcaa gggtggagag gagtagcctg
     3781 tggttggtgg ccaatgctgc cacctcagac catgctgcca gtgagggcca tgtctgagtc
     3841 tgtggctatg cagaggcaag aataggtgtc catatctgag gctcaatgcc actagagagc
     3901 aagggaaaac cctgatgggg cagcctctcg tgaccacgtg gctgtccaag gctgtgcaga
     3961 acaggaccaa cccttcactt gctgcagccc tctagagagc tggcccttcg ccttgcccag
     4021 gcaacactgc ggagcttgtc ctggaagtag gtgagttggg cctctatgtg agggagagct
     4081 gatcctgcta ctcctctgcc atgaggtggc ctgggcaaag ggtgtgtgag tgtgggagag
     4141 ctgacccttc acttggctgt tgtggggtgg ctggggcaca gagatgcctc ccttgtagcc
     4201 tccaccagtc aggaaagctg cctatagggt aataagtaca gggaaaacta gcctggcccc
     4261 tcacctcctg cagcactggg gagagtgggc ccacaccttg actgggcaga acagagcact
     4321 taactctgga gacatgggtg ctgtcagctg accctgcctc ttgccagtag cagcactggg
     4381 tggcctaacc agagcagtgc tggagagctc gccctggtga gcaggtaagg gagagctggc
     4441 aggctgagca gctcagcagc ccgcaggccc aggtccaggg ctctgaattt gtccatcccc
     4501 aaaatccata ccatctgcaa atcgttggga tgcatgaaca agccagtcct gctgttccaa
     4561 agctgtagga gtcccggtga ggatcctgta gtgatgatgt cacagaaacc aaagacctca
     4621 agccagacca atgactcatt gcagtggaca tttacaagga aagatgtgtg gacagaaggt
     4681 ttactgtgag actcactgtg acaccacagc ttcctcgatg aaatgtttct atgctttgct
     4741 ttttatttgt tgtttgtttt ttatttgggg ggagattgca agggtagaga gtggatatga
     4801 ggggacaagg agatgagtgg gactggggtg catgatggga aactcagaat caataaaaac
     4861 tttaaaaaaa aaaaaaaaaa aaaaa
//
