LOCUS       NM_025662               4744 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            K (Pigk), transcript variant 1, mRNA.
ACCESSION   NM_025662 XM_992234 XM_992257 XM_992285 XM_992302
VERSION     NM_025662.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Chen,X., Yin,W., Chen,S., Zhang,W., Li,H., Kuang,H., Zhou,M.,
            Teng,Y., Zhang,J., Shen,G., Liang,D., Li,Z., Hu,B. and Wu,L.
  TITLE     Loss of PIGK function causes severe infantile encephalopathy and
            extensive neuronal apoptosis
  JOURNAL   Hum Genet 140 (5), 791-803 (2021)
   PUBMED   33392778
REFERENCE   3  (bases 1 to 4744)
  AUTHORS   Boutlis,C.S., Riley,E.M., Anstey,N.M. and de Souza,J.B.
  TITLE     Glycosylphosphatidylinositols in malaria pathogenesis and immunity:
            potential for therapeutic inhibition and vaccination
  JOURNAL   Curr Top Microbiol Immunol 297, 145-185 (2005)
   PUBMED   16265905
  REMARK    Review article
REFERENCE   4  (bases 1 to 4744)
  AUTHORS   Eisenhaber,B., Maurer-Stroh,S., Novatchkova,M., Schneider,G. and
            Eisenhaber,F.
  TITLE     Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and
            post-translational transfer to proteins
  JOURNAL   Bioessays 25 (4), 367-385 (2003)
   PUBMED   12655644
  REMARK    Review article
REFERENCE   5  (bases 1 to 4744)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   6  (bases 1 to 4744)
  AUTHORS   Yu,J., Nagarajan,S., Knez,J.J., Udenfriend,S., Chen,R. and
            Medof,M.E.
  TITLE     The affected gene underlying the class K
            glycosylphosphatidylinositol (GPI) surface protein defect codes for
            the GPI transamidase
  JOURNAL   Proc Natl Acad Sci U S A 94 (23), 12580-12585 (1997)
   PUBMED   9356492
  REMARK    GeneRIF: The protein encoded by the human ortholog catalyzes the
            final step in glycosylphosphatidylinositol (GPI) anchoring of cell
            surface proteins, the transamidation reaction.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK077893.1 and BC060175.1.
            
            On Jun 19, 2009 this sequence version replaced NM_025662.4.
            
            Transcript Variant: This variant (1) lacks an alternate exon in the
            coding region compared to variant 1. The resulting protein (isoform
            1) is shorter but has the same N- and C-termini compared to isoform
            2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC060175.1, SRR1660811.222269.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1368              AK077893.1         2-1369
            1369-4744           BC060175.1         1355-4730
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3"
     gene            1..4744
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class K"
                     /db_xref="GeneID:329777"
                     /db_xref="MGI:MGI:1913863"
     exon            1..128
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..1223
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /EC_number="3.-.-.-"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; GPI transamidase; GPI-anchor transamidase;
                     phosphatidylinositol-glycan biosynthesis class K protein"
                     /codon_start=1
                     /product="GPI-anchor transamidase isoform 1 precursor"
                     /protein_id="NP_079938.1"
                     /db_xref="CCDS:CCDS17919.1"
                     /db_xref="GeneID:329777"
                     /db_xref="MGI:MGI:1913863"
                     /translation="MAAPCFLTLRVATLAALALLSLGSSAAGHIEDQAEQFFRSGHTN
                     NWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNARNPKPATV
                     FSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRVPPSTPRSKRLLSDDRSNILIY
                     MTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSP
                     NIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPASQTNMNDLFQVCP
                     KSLCVSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTEKISLQWDSQVVDSSSKEDG
                     TAEERMGPLKYAEQLPVAQIIHQKPKPRDWHPPGGFILGLWALIIMVFFKTYGIKHMK
                     FIF"
     exon            129..182
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            183..274
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            275..410
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            411..522
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            523..619
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            620..737
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            738..848
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            849..1021
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1106
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..4726
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1518..1523
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /note="hexamer: AGTAAA"
     polyA_site      1562
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
                     /note="major polyA site"
     polyA_site      1636
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
     polyA_site      3162
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
     polyA_site      4604
                     /gene="Pigk"
                     /gene_synonym="3000001O05Rik; Gm38470; PIG-K"
ORIGIN      
        1 ccctttgctc ttcggggcgt gagaagccgg caaacatggc ggccccctgc ttcctcactc
       61 tgcgggtggc taccctggcc gccttggcgc tcttgtctct cggcagctct gccgctggac
      121 acatcgagga tcaagccgaa cagttcttta gaagtggaca cacaaataac tgggctgttt
      181 tggtgtgcac atcccgattc tggtttaact accgacatgt tgcaaatact ctttctgttt
      241 atagaagcgt caagaggcta ggtatcccgg acagtcacat tgtcctgatg cttgctgatg
      301 acatggcgtg caatgctcgg aaccccaagc cagccacagt gttcagccac aagaacatgg
      361 agctcaatgt gtatggagac gatgtggaag tggactacag aagctatgag gtaactgtgg
      421 agaacttttt aagggtattg accgggaggg ttccacccag tacccctcgc tcaaagcgtc
      481 ttctttccga cgacagaagc aatatcctca tttatatgac aggtcatgga gggaatggtt
      541 tcttgaaatt ccaagattct gaagaaatca ccaacataga acttgcagat gcgtttgaac
      601 agatgtggca gaagagacgc tacaatgagc tgctgttcat tattgacact tgccagggcg
      661 cgtccatgta cgagcggttc tactctccta acatcatggc cttggctagt agccaggtgg
      721 gagaggattc gctgtcgcac cagcctgacc ctgcgattgg agttcatctt atggataggt
      781 acacgtttta tgtcttggaa tttttggaag aaattaatcc agctagccaa actaacatga
      841 acgacctttt tcaagtgtgt cccaaaagtc tctgtgtgtc gacccctgga catcgcactg
      901 accttttcca gcgagatcca aaaaatgtcc tgatcaccga tttcttcgga agtgtgcgca
      961 aggtggaaat cacaacagag aagatcagtt tgcagtggga ttcacaagtc gtggacagca
     1021 gttctaaaga agacggcacg gccgaggagc gcatgggacc tctcaagtat gctgagcagc
     1081 tcccggtggc tcagataata caccagaagc caaagccgag agactggcac cctcccggag
     1141 gcttcatcct ggggctgtgg gcgctcatca tcatggtctt cttcaagacc tatgggatca
     1201 agcatatgaa gttcattttc taagactcaa ggatgtatga ggaagaatga atggaagact
     1261 gcagccttgg agcaaaactt aggttttcat atgtttttaa atatgttgct tttgtgtaaa
     1321 tttgggaaga aaattaggaa aattgaatat taatgaactt ttgactttaa aacataatat
     1381 tgttaatgca cttgtttact ggccacagaa tatatctttc attgtgtttg tgtgtttgag
     1441 ttatagaaag cagagagcaa tgacattgga actttatgct taattctttt taccctcctc
     1501 tcattgtaaa agtgagtagt aaaggtgtta gttcccacta aaggaaatta cttccgttca
     1561 taggtttgtg tgtgctgtct tggtctgttt atctatggac atatgttccc aaccatacag
     1621 aatatgccat ctctaatctt tggtgtttac aatgcattat taacctttct ccgccctcgg
     1681 acatccttca ggatatactg tgagcctgac ttggatgcac ttgcctacag tccaggctgt
     1741 tcaggaggcc gagtcgattt ttgagccctg ggggtgttga aggtcagcct gggcaacaaa
     1801 caacaaccaa cagaagagag aaacaccacg gaatgcaccg cccactctat ggtggtcacg
     1861 tgacagctcc tgagggaccc ttacctgttc ctagagttcc cttgggctca ctctgggata
     1921 gtaatgcctc tggaattctg cagctttact cgcacaggaa gatggagaca cagctttact
     1981 cgcacaggaa gatggagaca cagctttact cgcacaggaa gatggagaca cagctttact
     2041 cgcacaggaa gatggagaca cagctttact cgcacaggaa gatggagaca cagctttaat
     2101 tgcacaggaa gatggaaaca cagctttact cgcacaggaa gatggagaca cagctttact
     2161 cgcacaggaa gatggagaca cagctttact cgcacaggaa gatggagaca cagctttact
     2221 cgcacaggaa gatggagaca cagctttact cgcacaggaa gatggagaca cagctttact
     2281 cgcacaggaa gatggagaca cagctttact cgcacaggaa gatggagaca cagctttact
     2341 cgcacaggaa gatggagaca cagctttact cgcacaggaa gatggagaca cagctttact
     2401 cgcacaggaa gatggagaca cagctttact cgcacaggaa gatggagaca cagctttact
     2461 cgcacaggaa gatggagaca cagctttact cgcacaggaa gatggagaca cagctttact
     2521 cgcacaggaa gatggagaca cagctttact cgcacaggaa gatggagaca cagctttact
     2581 cgcacaggaa gatggagaca cagctttact cgcacaggaa gatggagaca cagctttact
     2641 cgcacaggaa gatggagaca cagctttaat cgcacaggaa gatggagaca cagctttggt
     2701 agctgtttat ctagggaagt catgcctctg agaattttta gtggtaaccc ctcagagaaa
     2761 catgccctat tactttttat ttttaagcat actgtgaact tctagaattg attgtatctc
     2821 ttcagttttt gtcctgaaag agacagattg ctgacagctg gatctatgct gtccctgagg
     2881 acagccccat aaactggatg acatacatgg agcacacaca ctgaaggccc cgccttcatg
     2941 tctgatggat cagtaagaca catccaatac tgactcttct aggatgtcag tcacctccac
     3001 atcttcctgc ggtgaaaaga tggccaagct tgtgttttgt gaccattctc agacactctt
     3061 ttctctttca gactttggca tcagagagcc gtgctggggt gggctgtgcc tatagattta
     3121 ctattcatgt ctgaggagaa tcttaggtgt ttcatccaag aatctatatg ttcatctgtt
     3181 ttctttacaa atactgtttt ctaattgtag gtaacaatta agaagtcttc taatcccctg
     3241 gcaataggca gatacacctt gtttgtgata ttgctctttt gtcagtcagg ggcagagcac
     3301 atggcagtcc ttgttcatcc tagcttgttg cctgccctgg tggccaaagt tgtgtcatgc
     3361 ctaggacaca taaaaatcaa atcatcaaat caaattgagg caccttggga ggaagacagt
     3421 agacagaggc ctgtggacca tggaccacgt gccacccaca gacctgctct ctgatgtatg
     3481 acacaggtgg gctgtatata cccacccact gtgcatagac aggatttcat ccaggggccc
     3541 accagcttca tggcagcctg ctctgcagga gtcaccacga tgtgttagtt agtatgtgct
     3601 agtcaggtgg gagactcaag ggtggagagg agtagcctgt ggttggtggc caatgctgcc
     3661 acctcagacc atgctgccag tgagggccat gtctgagtct gtggctatgc agaggcaaga
     3721 ataggtgtcc atatctgagg ctcaatgcca ctagagagca agggaaaacc ctgatggggc
     3781 agcctctcgt gaccacgtgg ctgtccaagg ctgtgcagaa caggaccaac ccttcacttg
     3841 ctgcagccct ctagagagct ggcccttcgc cttgcccagg caacactgcg gagcttgtcc
     3901 tggaagtagg tgagttgggc ctctatgtga gggagagctg atcctgctac tcctctgcca
     3961 tgaggtggcc tgggcaaagg gtgtgtgagt gtgggagagc tgacccttca cttggctgtt
     4021 gtggggtggc tggggcacag agatgcctcc cttgtagcct ccaccagtca ggaaagctgc
     4081 ctatagggta ataagtacag ggaaaactag cctggcccct cacctcctgc agcactgggg
     4141 agagtgggcc cacaccttga ctgggcagaa cagagcactt aactctggag acatgggtgc
     4201 tgtcagctga ccctgcctct tgccagtagc agcactgggt ggcctaacca gagcagtgct
     4261 ggagagctcg ccctggtgag caggtaaggg agagctggca ggctgagcag ctcagcagcc
     4321 cgcaggccca ggtccagggc tctgaatttg tccatcccca aaatccatac catctgcaaa
     4381 tcgttgggat gcatgaacaa gccagtcctg ctgttccaaa gctgtaggag tcccggtgag
     4441 gatcctgtag tgatgatgtc acagaaacca aagacctcaa gccagaccaa tgactcattg
     4501 cagtggacat ttacaaggaa agatgtgtgg acagaaggtt tactgtgaga ctcactgtga
     4561 caccacagct tcctcgatga aatgtttcta tgctttgctt tttatttgtt gtttgttttt
     4621 tatttggggg gagattgcaa gggtagagag tggatatgag gggacaagga gatgagtggg
     4681 actggggtgc atgatgggaa actcagaatc aataaaaact ttaaaaaaaa aaaaaaaaaa
     4741 aaaa
//
