LOCUS       NM_018889               4490 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            B (Pigb), transcript variant 1, mRNA.
ACCESSION   NM_018889
VERSION     NM_018889.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Takahashi,M., Inoue,N., Ohishi,K., Maeda,Y., Nakamura,N., Endo,Y.,
            Fujita,T., Takeda,J. and Kinoshita,T.
  TITLE     PIG-B, a membrane protein of the endoplasmic reticulum with a large
            lumenal domain, is involved in transferring the third mannose of
            the GPI anchor
  JOURNAL   EMBO J 15 (16), 4254-4261 (1996)
   PUBMED   8861954
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158997.2.
            
            On Aug 13, 2022 this sequence version replaced NM_018889.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D84436.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-196               AC158997.2         181570-181765       c
            197-332             AC158997.2         180731-180866       c
            333-450             AC158997.2         178812-178929       c
            451-555             AC158997.2         176595-176699       c
            556-686             AC158997.2         174161-174291       c
            687-827             AC158997.2         171674-171814       c
            828-879             AC158997.2         165337-165388       c
            880-1091            AC158997.2         164383-164594       c
            1092-1156           AC158997.2         163394-163458       c
            1157-1370           AC158997.2         159978-160191       c
            1371-1551           AC158997.2         159499-159679       c
            1552-4490           AC158997.2         155465-158403       c
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 40.08 cM"
     gene            1..4490
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class B"
                     /db_xref="GeneID:55981"
                     /db_xref="MGI:MGI:1891825"
     exon            1..196
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..1695
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /EC_number="2.4.1.-"
                     /note="involved in GPI-anchor biosynthesis; GPI-MT-III;
                     GPI mannosyltransferase III; phosphatidylinositol-glycan
                     biosynthesis class B protein; phosphatidylinositol glycan,
                     class B"
                     /codon_start=1
                     /product="GPI mannosyltransferase 3"
                     /protein_id="NP_061377.2"
                     /db_xref="CCDS:CCDS40687.1"
                     /db_xref="GeneID:55981"
                     /db_xref="MGI:MGI:1891825"
                     /translation="MESQAADYNPASRNLHGSSGEMKLRRRKSRQYVSAQEKRSPRRG
                     LLGENTYLVLFTIALRILNCFLVQTSFVPDEYWQSLEVAHRMVFSYGYLTWEWTERLR
                     GYTYPLIFASIYKVLHLLGKDSVQFLIWIPRLGQALLSAVADIRLYSLLKQLENQEVA
                     QWVFLCQLCSWFTWYCCTRTLTNTMETSLTALALFYYPLEGSRSVNSVKYSLLVALAC
                     VVRPTALIPWVPLLFRHFYQEQRKLHLTLHHFLPVGFITFSLSLIIDRIFFGQWTLVQ
                     LNFLKFNVLQNLGTFYGSHPWHWYLSQGFPVVLGTHLPFFIHGCFLAPRRLHILLLTV
                     LWTLLVYSMLGHKEFRFIYPVLPFCMVFCGYSLAHLKTWRKAALSFLLLSNVPLAFYT
                     GLVHQRGTLDVMNHIQKVCPRGPDPASASVFIMMPCHSTPYYSHVHCPLSMRFLQCPP
                     DLTGKTQYLDEADMFYLNPLRWLQQEFHSNASLPTHLVTFNVLEKEINTFLTSGNYER
                     AATFFHTHWPERRTGSHIHVYERRLPGRVNTGGN"
     exon            197..332
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     exon            333..450
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     exon            451..555
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     exon            556..686
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     exon            687..827
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     exon            828..879
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1091
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1156
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1370
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1551
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..4490
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3166..3171
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /note="hexamer: AATAAA"
     polyA_site      3192
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /note="major polyA site"
     regulatory      4100..4105
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /note="hexamer: AATAAA"
     regulatory      4116..4121
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /note="hexamer: ATTATA"
     polyA_site      4121
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
     polyA_site      4158
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
     regulatory      4461..4466
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
                     /note="hexamer: AATAAA"
     polyA_site      4490
                     /gene="Pigb"
                     /gene_synonym="Pig-b"
ORIGIN      
        1 agtggcatgt gtccgcctac aagaaagtga ggggcgggca ccgtgggctg caggtagccg
       61 gggaggatgg agtcccaggc cgccgactat aacccggcct cccgcaatct ccacggcagc
      121 tcgggagaaa tgaagctgcg gaggaggaag tccaggcagt acgtgagcgc gcaggagaaa
      181 cgctctccgc gccgtggtct tcttggagaa aacacttacc tggtcttgtt taccatcgct
      241 ctgcgaatac ttaactgctt cttagtgcag acaagctttg ttcctgatga atactggcag
      301 tccctcgaag ttgcacaccg catggttttc agttatggtt atttgacctg ggaatggacg
      361 gagcgattga gaggctacac ttaccccctc atcttcgcca gtatttacaa ggttctgcat
      421 cttctgggga aagacagtgt tcaattcctg atctggattc ccagacttgg ccaagctctt
      481 ctgtctgctg tagcggatat aaggctctac tccctcctga agcagctgga aaaccaggaa
      541 gtggcacaat gggtgttcct ctgccagctg tgctcctggt tcacatggta ctgctgcaca
      601 cggaccctca cgaacaccat ggagacctcc ctcactgccc tcgccctttt ctactatcct
      661 ctggaaggct cgaggtccgt gaacagcgtc aagtactcgc tcctggtggc acttgcttgt
      721 gtggtccgcc ccacagctct tatcccatgg gtgccgctgc tcttcagaca tttctaccaa
      781 gaacagagaa agctccatct gactctgcat cattttttac ctgtaggatt tataaccttc
      841 agtttgtctc tgataattga ccgtatcttt tttggccaat ggacgctggt ccaattaaat
      901 ttcttgaaat ttaacgtgct gcagaaccta gggacgtttt atggctccca tccatggcac
      961 tggtacctca gtcaagggtt cccagttgtc ctggggaccc acttaccttt cttcattcac
     1021 ggttgcttcc tagccccaag gaggctccac atactgctgc tgaccgtgct gtggacactg
     1081 ctggtataca gcatgttggg ccacaaagaa ttcaggttta tctatccagt tttaccattc
     1141 tgcatggtgt tctgtggtta ctcactagcc cacctgaaga catggaggaa ggcagccctg
     1201 agcttcctgc tcttgtcaaa tgtgccactg gcattctaca ctggcctagt tcatcagaga
     1261 ggcaccctgg atgtcatgaa tcacattcag aaagtctgcc cccgaggtcc tgacccagct
     1321 tcggcctcag tattcataat gatgccctgc cactccacgc cttactacag ccatgttcac
     1381 tgcccactgt ccatgcggtt tctccagtgt cccccagact tgactggaaa aactcagtat
     1441 cttgatgaag cagatatgtt ctacttaaac cccttaagat ggttacaaca agaatttcac
     1501 agcaatgcgt cactccccac tcacttggtc accttcaatg ttctggagaa ggagatcaat
     1561 accttcctca cctcagggaa ctacgagagg gccgctacat tcttccacac tcactggcca
     1621 gagcgccgaa ctggaagtca catacacgtc tacgagcgaa ggttacccgg cagggtcaac
     1681 acaggaggga actgaactgt aaggacttct gcagagacgt tggcataact gagagaaact
     1741 gtaggtgacc gtgaaatgag aggaagccac tggggacagc tgtcccatac actgtcctga
     1801 ggcattctca gagaacacaa atacggcgcc agacttgaga gagaccttta gttttcctca
     1861 cggtgcagtt tattgtttct tccatagcat tcaggtgtgc aagagagttc acaagacaag
     1921 ccagccagct cttcacaaac caacgggctc ttgaacccac agagagacaa aagcgtgaga
     1981 gtagagatgt tagcgcggag tcccgcctca gcacagtcct caggccctgc tgcacacctc
     2041 cacacggcac gaaatgcctg tgtgagctga gctcagctcc tcaaaccaag ccctgtttca
     2101 tgcacagact atttcatgcc tggcttcctt aataaaacta ccatttcccc cacccccccc
     2161 cagcaggaaa aaaagttttt gtttttaaca aacctaagct acaggaaaat gcaagttagt
     2221 aagctttgct tttgcataat ataaagacag tcatggggag ttcatctcaa catcaaacca
     2281 agtagatagc atccacagct cttaacttcc taacttaatc acaaatcagt acttaggatc
     2341 gaaaggtaac tgccccagtt ctagctcaag atttgccgtg tgtcttccgt tagcgcgacc
     2401 gtatttttgc catttacctt ccctttcata aggctgtgga tgaaggtgct tctgctcttg
     2461 ttttcggtgc ctggcgtttt caatagttac catacgttgc tttttatcag gtcgactgaa
     2521 agtagtaaac acattttttt aatctatgtt agagttcagt tttctaaaag taaaaagaac
     2581 aatgtttttc cccacttatc tacctgaaca ctgcaaaaag ataaaacaga aaataacatc
     2641 ctactctaat tccaggaaaa aggggtatta tttcaacact gaaggaaaaa gggcagacat
     2701 caatactccc gcactggccg gaaggggagg cctgacaggt gacgaggagc aggaatgtaa
     2761 ccataaaata atgaggcgtt tccgttatca ctccaggaag cataagaaca accagcctcg
     2821 tctccatcgg ctttatccct caatgagaga agagacacag ctcgcagagc agtctccaac
     2881 cttcccccgt tccaggcaag tgcagtgagc aggcactgct gtcttcccac tctgcctgct
     2941 ggctcctcca ctgccttacg ccaggacctg cacacaagcg ctctgcagcg ctcagctcta
     3001 agcacctgac ttgacatctc tgggttcacg tgcatcactc tgcactcttc tcgcttagcc
     3061 ttctctctaa ccagcacaga tagcattgtc ccctgcacta gaccaatggg gactggcttc
     3121 gaagtgagta gaaatggcca tgtacctacc aacccgtcct ctccaaataa atgtcaacag
     3181 atacctccaa aatcaagttg cttctggttc ctcttgctta gagcttcctt ctggagttct
     3241 gagtaatctc agcatccaac agtgaagtca ttccaggtga acctacacca cttactttct
     3301 acttccctta cacaaggtag aagtgtctgg ctatgtgact tgttgacaat gtatcttgtc
     3361 tgctcctcac cacctctgat gacctcattc aaactcccat cagtttctca tgtgcagacc
     3421 accacagctg gcttcacacc ttgccaatca accctcttgg ctgctgccag tcatctagag
     3481 gactaggcac catcccaccc cgtgacaaga tccttcagaa attctccatc tcacaagaca
     3541 ggcacttgag ggcaaaaggg agaccttcct gatcacgcca gctcacctgg tttctgtaaa
     3601 gtaggtctcc atctctccaa cctctgcaaa ggaccttccg ttccagggag gccctgctct
     3661 atccacgcac caaccctcac ttctctccca gaagctagtt tcatagcaag tttagatgca
     3721 aggtcacttc aatcatgaag attttctaac catctctaga caacctgagg tcactacacc
     3781 ttcatgcctc tctctgcaca cctaactctt gaacagcaag aagttatcca cgcttcctta
     3841 aagcagcctc caacattgct cattaaagca tccgcttcag gaactaaaga catcagaaag
     3901 cagtctcatt attttatagg cacactgcat ttattcataa agatggaact tgactctcaa
     3961 aagtaaacag acatttacag ttacagaata aaaagctgaa ggacaacaga cagtcttgaa
     4021 gaacattatg ggattttgtg gctcctgagc gtccagcacc cttctcagtt ttaaccctgt
     4081 aaccagtact cctaaactca ataaacctga cagtgattat agctgggcag ctccacatga
     4141 cccgggcttc acactaaagt ctttgtttct agcttcctaa gttaaagatt aaagggaaat
     4201 aatttcactt aattatataa actatactga aaaaagctag attggctcaa ttaaattgtg
     4261 attgtatgta tgtatgtgtg tatgtgtatg tgtatacata cacacataca tacatacata
     4321 cacatacaca tcctttaaat ttttaaagta gaaattcaat catttgttca agtctaagtg
     4381 tttgacatgg actttttttc agtttgcaat aattataatt tataatataa tttccaaaag
     4441 ctcaaagtgt taaaagataa aataaaatgg tatgcactgt ttagaagcta
//
