LOCUS       NM_011081               3563 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus phosphatidylinositol glycan anchor biosynthesis, class
            A (Piga), transcript variant 1, mRNA.
ACCESSION   NM_011081 XM_136136
VERSION     NM_011081.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Murakami,Y., Umeshita,S., Imanishi,K., Yoshioka,Y., Ninomiya,A.,
            Sunabori,T., Likhite,S., Koike,M., Meyer,K.C. and Kinoshita,T.
  TITLE     AAV-based gene therapy ameliorated CNS-specific GPI defect in mouse
            models
  JOURNAL   Mol Ther Methods Clin Dev 32 (1), 101176 (2023)
   PUBMED   38225934
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Jangid,A., Fukuda,S., Seki,M., Suzuki,Y., Taylor,T.D., Ohno,H. and
            Prakash,T.
  TITLE     Gut microbiota alternation under the intestinal epithelium-specific
            knockout of mouse Piga gene
  JOURNAL   Sci Rep 12 (1), 10812 (2022)
   PUBMED   35752737
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3563)
  AUTHORS   Kandasamy,L.C., Tsukamoto,M., Banov,V., Tsetsegee,S., Nagasawa,Y.,
            Kato,M., Matsumoto,N., Takeda,J., Itohara,S., Ogawa,S., Young,L.J.
            and Zhang,Q.
  TITLE     Limb-clasping, cognitive deficit and increased vulnerability to
            kainic acid-induced seizures in neuronal
            glycosylphosphatidylinositol deficiency mouse models
  JOURNAL   Hum Mol Genet 30 (9), 758-770 (2021)
   PUBMED   33607654
REFERENCE   4  (bases 1 to 3563)
  AUTHORS   Lukacs,M., Blizzard,L.E. and Stottmann,R.W.
  TITLE     CNS glycosylphosphatidylinositol deficiency results in delayed
            white matter development, ataxia and premature death in a novel
            mouse model
  JOURNAL   Hum Mol Genet 29 (7), 1205-1217 (2020)
   PUBMED   32179897
REFERENCE   5  (bases 1 to 3563)
  AUTHORS   Kawagoe,K., Kitamura,D., Okabe,M., Taniuchi,I., Ikawa,M.,
            Watanabe,T., Kinoshita,T. and Takeda,J.
  TITLE     Glycosylphosphatidylinositol-anchor-deficient mice: implications
            for clonal dominance of mutant cells in paroxysmal nocturnal
            hemoglobinuria
  JOURNAL   Blood 87 (9), 3600-3606 (1996)
   PUBMED   8611683
REFERENCE   6  (bases 1 to 3563)
  AUTHORS   Yu,J. and Medof,M.E.
  TITLE     Characterization of the murine PIG-A promoter region high
            constitutive PIG-A gene expression in brain
  JOURNAL   Biochem Biophys Res Commun 215 (2), 497-503 (1995)
   PUBMED   7487983
REFERENCE   7  (bases 1 to 3563)
  AUTHORS   Yu,J., Nagarajan,S., Liu,J., Young,N. and Medof,M.E.
  TITLE     Cloning and characterization of the mouse PIG-A gene
  JOURNAL   Biochim Biophys Acta 1255 (3), 344-350 (1995)
   PUBMED   7734452
REFERENCE   8  (bases 1 to 3563)
  AUTHORS   Kawagoe,K., Takeda,J., Endo,Y. and Kinoshita,T.
  TITLE     Molecular cloning of murine pig-a, a gene for GPI-anchor
            biosynthesis, and demonstration of interspecies conservation of its
            structure, function, and genetic locus
  JOURNAL   Genomics 23 (3), 566-574 (1994)
   PUBMED   7851884
REFERENCE   9  (bases 1 to 3563)
  AUTHORS   Ware,R.E., Howard,T.A., Kamitani,T., Chang,H.-M., Yeh,E.T.H. and
            Seldin,M.F.
  TITLE     Chromosomal assignment of genes involved in
            glycosylphosphatidylinositol anchor biosynthesis: implications for
            the pathogenesis of paroxysmal nocturnal hemoglobinuria
  JOURNAL   Blood 83 (12), 3753-3757 (1994)
   PUBMED   8204896
REFERENCE   10 (bases 1 to 3563)
  AUTHORS   Takeda,J., Miyata,T., Kawagoe,K., Iida,Y., Endo,Y., Fujita,T.,
            Takahashi,M., Kitani,T. and Kinoshita,T.
  TITLE     Deficiency of the GPI anchor caused by a somatic mutation of the
            PIG-A gene in paroxysmal nocturnal hemoglobinuria
  JOURNAL   Cell 73 (4), 703-711 (1993)
   PUBMED   8500164
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732475.6.
            
            On Feb 1, 2022 this sequence version replaced NM_011081.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D26047.1, AK161026.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AL732475.6         110092-110114
            24-803              AL732475.6         112858-113637
            804-936             AL732475.6         118206-118338
            937-1069            AL732475.6         118814-118946
            1070-1276           AL732475.6         119085-119291
            1277-3563           AL732475.6         121904-124190
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 76.49 cM"
     gene            1..3563
                     /gene="Piga"
                     /gene_synonym="Pig-a"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class A"
                     /db_xref="GeneID:18700"
                     /db_xref="MGI:MGI:99461"
     exon            1..23
                     /gene="Piga"
                     /gene_synonym="Pig-a"
                     /inference="alignment:Splign:2.1.0"
     exon            24..803
                     /gene="Piga"
                     /gene_synonym="Pig-a"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..1543
                     /gene="Piga"
                     /gene_synonym="Pig-a"
                     /EC_number="2.4.1.198"
                     /note="N-acetylglucosaminyl-phosphatidylinositol
                     biosynthetic protein; glcNAc-PI synthesis protein;
                     phosphatidylinositol-glycan biosynthesis class A protein;
                     phosphatidylinositol glycan, class A;
                     phosphatidylinositolglycan class A"
                     /codon_start=1
                     /product="phosphatidylinositol
                     N-acetylglucosaminyltransferase subunit A"
                     /protein_id="NP_035211.2"
                     /db_xref="CCDS:CCDS30522.1"
                     /db_xref="GeneID:18700"
                     /db_xref="MGI:MGI:99461"
                     /translation="MANRRGGGQGQPPSVSPSPGSSGNLSDDRTCTHNICMVSDFFYP
                     NMGGVESHIYQLSQCLIERGHKVITVTHAYGNRKGVRYLTNGLKVYYLPLRVMYNQST
                     ATTLFHSLPLLRYIFVRERITIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFA
                     DVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPD
                     PFRRHDSVITVVVVSRLVYRKGTDLLSGIIPELCQKYQELHFLIGGEGPKRIILEEVR
                     ERYQLHDRVQLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTKV
                     GGIPEVLPESLIILCEPSVKSLCDGLEKAIFQVKSGTLPAPENIHNVVKTFYTWRNVA
                     ERTEKVYERVSKETVLPMHKRLDRLISHCGPVTGYMFALLAVLSYLFLIFLQWMTPDS
                     FIDVAIDATGPRRAWTHQWPRDKKRDENDKISQSR"
     exon            804..936
                     /gene="Piga"
                     /gene_synonym="Pig-a"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1069
                     /gene="Piga"
                     /gene_synonym="Pig-a"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1276
                     /gene="Piga"
                     /gene_synonym="Pig-a"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..3563
                     /gene="Piga"
                     /gene_synonym="Pig-a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3531..3536
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Piga"
                     /gene_synonym="Pig-a"
                     /note="hexamer: AATAAA"
     polyA_site      3563
                     /gene="Piga"
                     /gene_synonym="Pig-a"
                     /note="major polyA site"
ORIGIN      
        1 agtggcggcc atgggactgg agggtgacag aagacacatt tcttaactgg cttgctctga
       61 gaactgatgc ttggaccacc tcagcatggc caatagaaga ggaggtgggc aaggccagcc
      121 tccttcagtg tcgccttccc cgggtagctc tggaaacctt tccgacgata ggacctgcac
      181 ccataatata tgcatggtgt cagacttttt ctacccaaac atgggaggcg tggaaagcca
      241 catttaccag ctctctcagt gcctcattga gagagggcac aaggtcataa ctgtcaccca
      301 tgcttatgga aatcgaaagg gcgtccgtta cctcaccaat ggcctcaaag tttactattt
      361 gcctctgaga gtcatgtaca accaatctac agccacgact ctctttcaca gtctgccatt
      421 gctcaggtac atatttgttc gggagagaat tacgataatc cattcccaca gttctttctc
      481 tgccatggcc catgatgctc tcttccacgc caagacaatg gggcttcaga cagtcttcac
      541 agaccattcc cttttcggat ttgctgatgt cagctcggtg cttacaaaca agcttctaac
      601 tgtgtctctt tgtgacacaa accacatcat ttgtgtctct tacactagta aggagaacac
      661 tgtactcaga gcagcactga atcctgaaat agtgtccgtc attcctaacg ctgtagatcc
      721 tactgacttc actccagacc catttaggag gcatgatagt gtaataactg ttgttgttgt
      781 cagcagactt gtttacagaa aagggactga tttgcttagt ggtataatac ctgaactctg
      841 tcagaaatac caagaattac atttcctaat tggaggagag ggaccaaaga gaatcatttt
      901 ggaagaagta cgggaaagat accaactaca tgacagggtg cagcttttgg gagctttaga
      961 acacaaggat gttagaaatg tcttagttca aggacacatt tttcttaaca cctccctcac
     1021 tgaagcgttc tgcatggcca tcgtggaagc tgccagttgt ggtttgcagg tcgtcagcac
     1081 aaaggttggt gggattcccg aagtccttcc ggaaagtctt attattctct gcgagccttc
     1141 agtaaaatct ttgtgtgacg gtctggaaaa agccattttc caagtgaagt cggggacatt
     1201 gccagctcca gaaaatatcc acaatgtagt gaagactttc tacacctggc ggaacgtggc
     1261 agagagaact gaaaaggtgt atgagcgagt gtcgaaggaa actgttttgc caatgcacaa
     1321 gagattggac aggctcatct cacactgtgg ccctgtgacg ggctacatgt ttgctttgct
     1381 ggctgtgctc agctatctct tcctcatttt cctgcagtgg atgactccag actctttcat
     1441 tgatgttgca atagatgcta ctgggccaag gagagcctgg actcatcaat ggcctcgtga
     1501 taagaaaaga gatgagaatg ataagatatc ccaaagcagg tagaagcaag cccaagttaa
     1561 caagtcctat tagatacttg caatagtcct gttagcattt gaaaggagtt ttgctcctgt
     1621 gttgggcttt agagttggat ttgctacctc tgtatggttc acttttgttg ttgcaaaaag
     1681 ataaacactt atgcaattcc tgagtgtggg aactccttgc acttctttaa aatttagggg
     1741 ataatggttt agccactcag gtaggacagt tttcagatta ctgagacccc cctaagcaga
     1801 gatgttttaa cattatattt tgggagcctt gggtgctgaa aggccaaatg tttctggatt
     1861 gtttttggcc aatttttcac attataccat taatccacat tcaccagatg tttacatgct
     1921 ttctttaggg aggtacaaca gttatgtgaa ctgttttaag gcaaggtcat gtatccttct
     1981 ctgggcatca ttgaagtcat gttcctagac acttactggg tccactcaag ggatacgtgg
     2041 aatgtgattt cttgagcaag accatctatt ggttgccagc atgtttgaac acctgctgtg
     2101 tatgaaaagt tgacctagat ggttcctgag actaaggact ggtcaacgag aagtggtaaa
     2161 actgtcaaca tggcgaaatg ctcaagctac tgaatgacag gagatcatcg ctggcataca
     2221 ccaagtacct ttctgacctg ttctatgtat atgcattctc ttaggaagag cccttctctc
     2281 tctctctctc tctctctctc tctctctctc tgtatgtgtg tgtgtgtgtg tgtgcgtgtg
     2341 tgtgtatgtg tgcacgcgtg cgtgtgtgtt gttgcttttt atgttctatg gattgaccga
     2401 tacaactgtt gatgcttgtg ctggccaacc ctctgctttt atagtgcaca gttcagtccc
     2461 ccataaatgc ctttatggtt tctaagcaga acagaaaagg tgtgctatct caaaaccagc
     2521 tgctcttcct gccctctgtg gaagcccctt cctgtcactc tggtcttgcc catctctgat
     2581 aagtattgcc agtgtgggga aaggcttttc tccagaatgg tttatttaga gcgtattaat
     2641 tctgaaactc gtcagagctc gcaggctttt tgtgggtgga atttctcaat cgtaattaag
     2701 gtttccagag ccatgggttt ttaaatccca aactgtatgt gatatgtatt attaatcctt
     2761 tgaactcttt cgcatgggtt tgtgtgggga acagactaaa atgagagcag cgagccactt
     2821 gtgggtgttc cattctgtca gtattgtgat tctgtacagg agtttttttt agcgacattt
     2881 gcaaaacttt taaaagagta tcttaacatg cctattactt ggttgccacc gatataaaag
     2941 agatatattt gtgtttggtt tctatggaaa caaatggtag agagtaattt ttaaaatata
     3001 gaagttaggg cttcttttat tggagaaaaa tgaccttgtc caaaccgttc agtcttattt
     3061 aaatctttat gcgctgagac aggcgtacag gttaacagtg ccaatatcca cggtagactg
     3121 aggcatgtgt aacacttggc tgtatttatt ctactctatt atttcttcta tgtttgcttc
     3181 tagcttttat taaaatatca ttaaatgctt cagttgttct ttatttaaga tgaacataga
     3241 attgaaaatt aatttgctct tgtgggcaaa tatgtaattt atgaattaaa attgttttcc
     3301 ttttttgaac aagtaaaaat aaaatttgtg tgacagaaac cattttcagg cttcctggat
     3361 accttagttg tcagtgttgt actggtcagg atgtgggaca ctcttccact gtgctactca
     3421 taattatctg aaatgaaatt agcgtggatg aggaatcgtt ggaaagatac tgtgttgctt
     3481 ttgatgtcat acacatgaaa ccagtgtgct aaaacatgct catgtccagt aataaagatg
     3541 agaacgctgt tttaatagtc tta
//
