LOCUS       NM_172453               3716 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant
            1, mRNA.
ACCESSION   NM_172453
VERSION     NM_172453.5
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Belmonte,F.R., Dedousis,N., Sipula,I., Desai,N.A., Singhi,A.D.,
            Chu,Y., Zhang,Y., Bannwarth,S., Paquis-Flucklinger,V.,
            Harrington,L., Shiva,S., Jurczak,M.J., O'Doherty,R.M. and
            Kaufman,B.A.
  TITLE     Petite Integration Factor 1 (PIF1) helicase deficiency increases
            weight gain in Western diet-fed female mice without increased
            inflammatory markers or decreased glucose clearance
  JOURNAL   PLoS One 14 (5), e0203101 (2019)
   PUBMED   31136580
  REMARK    GeneRIF: They also support a role for Pif1 in weight gain
            resistance and liver metabolic dysregulation during nutrient
            stress.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3716)
  AUTHORS   Rue,L., Banez-Coronel,M., Creus-Muncunill,J., Giralt,A.,
            Alcala-Vida,R., Mentxaka,G., Kagerbauer,B., Zomeno-Abellan,M.T.,
            Aranda,Z., Venturi,V., Perez-Navarro,E., Estivill,X. and Marti,E.
  TITLE     Targeting CAG repeat RNAs reduces Huntington's disease phenotype
            independently of huntingtin levels
  JOURNAL   J Clin Invest 126 (11), 4319-4330 (2016)
   PUBMED   27721240
REFERENCE   4  (bases 1 to 3716)
  AUTHORS   Bannwarth,S., Berg-Alonso,L., Auge,G., Fragaki,K., Kolesar,J.E.,
            Lespinasse,F., Lacas-Gervais,S., Burel-Vandenbos,F., Villa,E.,
            Belmonte,F., Michiels,J.F., Ricci,J.E., Gherardi,R., Harrington,L.,
            Kaufman,B.A. and Paquis-Flucklinger,V.
  TITLE     Inactivation of Pif1 helicase causes a mitochondrial myopathy in
            mice
  JOURNAL   Mitochondrion 30, 126-137 (2016)
   PUBMED   26923168
  REMARK    GeneRIF: pif1-/- animals develop a mitochondrial myopathy with
            respiratory chain deficiency.
REFERENCE   5  (bases 1 to 3716)
  AUTHORS   Kazak,L., Reyes,A., Duncan,A.L., Rorbach,J., Wood,S.R.,
            Brea-Calvo,G., Gammage,P.A., Robinson,A.J., Minczuk,M. and
            Holt,I.J.
  TITLE     Alternative translation initiation augments the human mitochondrial
            proteome
  JOURNAL   Nucleic Acids Res 41 (4), 2354-2369 (2013)
   PUBMED   23275553
REFERENCE   6  (bases 1 to 3716)
  AUTHORS   Reynolds,G.E., Gao,Q., Miller,D., Snow,B.E., Harrington,L.A. and
            Murnane,J.P.
  TITLE     PIF1 disruption or NBS1 hypomorphism does not affect chromosome
            healing or fusion resulting from double-strand breaks near
            telomeres in murine embryonic stem cells
  JOURNAL   DNA Repair (Amst) 10 (11), 1164-1173 (2011)
   PUBMED   21945094
  REMARK    GeneRIF: murine cells have evolved mechanisms to ensure the
            functional redundancy of Pif1 or Nbs1 in the regulation of
            chromosome healing.
REFERENCE   7  (bases 1 to 3716)
  AUTHORS   Snow,B.E., Mateyak,M., Paderova,J., Wakeham,A., Iorio,C.,
            Zakian,V., Squire,J. and Harrington,L.
  TITLE     Murine Pif1 interacts with telomerase and is dispensable for
            telomere function in vivo
  JOURNAL   Mol Cell Biol 27 (3), 1017-1026 (2007)
   PUBMED   17130244
  REMARK    GeneRIF: Murine telomere homeostasis or genetic stability does not
            depend on mPif1.
REFERENCE   8  (bases 1 to 3716)
  AUTHORS   Bulfone,A., Carotenuto,P., Faedo,A., Aglio,V., Garzia,L.,
            Bello,A.M., Basile,A., Andre,A., Cocchia,M., Guardiola,O.,
            Ballabio,A., Rubenstein,J.L. and Zollo,M.
  TITLE     Telencephalic embryonic subtractive sequences: a unique collection
            of neurodevelopmental genes
  JOURNAL   J Neurosci 25 (33), 7586-7600 (2005)
   PUBMED   16107646
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC135108.3.
            
            On May 20, 2019 this sequence version replaced NM_172453.4.
            
            Transcript Variant: This variant (1) represents the longest
            protein-coding transcript. Variants 1-3 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY498715.1, AK049353.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG   :: upstream AUG and CDS
                                                  extension is not conserved
            gene product(s) localized to mito. :: inferred from homology
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1221              AC135108.3         101848-103068
            1222-1354           AC135108.3         103679-103811
            1355-1480           AC135108.3         103889-104014
            1481-1634           AC135108.3         104125-104278
            1635-1749           AC135108.3         104387-104501
            1750-1856           AC135108.3         104622-104728
            1857-1996           AC135108.3         106394-106533
            1997-2103           AC135108.3         106798-106904
            2104-2191           AC135108.3         107936-108023
            2192-2337           AC135108.3         108131-108276
            2338-2529           AC135108.3         109061-109252
            2530-3716           AC135108.3         109455-110641
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 35.4 cM"
     gene            1..3716
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /note="PIF1 5'-to-3' DNA helicase"
                     /db_xref="GeneID:208084"
                     /db_xref="MGI:MGI:2143057"
     exon            1..1221
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     CDS             676..2589
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     helicase-like protein; ATP-dependent DNA helicase PIF1;
                     Pif1/Rrm3 DNA helicase-like protein; PIF1 homolog; PIF1
                     5'-to-3' DNA helicase homolog; DNA repair and
                     recombination helicase PIF1"
                     /codon_start=1
                     /product="ATP-dependent DNA helicase PIF1 isoform 1"
                     /protein_id="NP_766041.2"
                     /db_xref="CCDS:CCDS23295.1"
                     /db_xref="GeneID:208084"
                     /db_xref="MGI:MGI:2143057"
                     /translation="MPSSTEAATDECDDAELRCRVAVEELSPGGQPRKRQALRAAELS
                     LGRNERRELMLRLQAPGPTGRPRCFPLRAVRLFTRFAATGRSTLRLPTDGVPGAGSVQ
                     LLLSDCPPERLRRFLRTLRLKLAVAPGPGPASARAQLLGPRPRDFVTISPVQPEELQR
                     AAATKAPDSALEKRPMESQTSTEAPRWPLPVKKLRMPSTKPKLSEEQAAVLRMVLKGQ
                     SIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAP
                     LAQCMALANRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQKKPFGGIQL
                     IICGDFLQLPPVTKGSQQPQFCFQAKSWRRCVPVILELTEVWRQADQTFISLLQAVRL
                     GRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNEKWLKALPGDVHSFEAID
                     SDPELSRTLDAQCPVSRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRGLP
                     RVRFLCGITEVIRTDRWTVQVTGGQYLSRQQLPLQLAWAISIHKSQGMSLDCVEISLG
                     RVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLHFYATLRQGRGLSLESQDDE
                     EANSDLENMDPNL"
     exon            1222..1354
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1480
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1634
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1749
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     exon            1750..1856
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..1996
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     exon            1997..2103
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2191
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     exon            2192..2337
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     exon            2338..2529
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     exon            2530..3716
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3695..3700
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /note="hexamer: AATAAA"
     polyA_site      3716
                     /gene="Pif1"
                     /gene_synonym="4631410M14"
                     /note="major polyA site"
ORIGIN      
        1 cttcctgctg aagctttctg ccggccggtt cagatcagct ttttcatctg aaagcaacga
       61 gtctatcgga tccttgaggt gggaggcaaa gaacgcgatt attttagtga tcctcgctgg
      121 gagaggtaca gattcgtggg tcagacggag ggacaatgga ttcctgggcc tggaggttcc
      181 agacattccc taatcattta ccctttccaa agcactggaa ccacactgac cctgatacct
      241 actaattggt tattgaaggg ggtgtgcaag tctcagcctg ttttcacttc cagccagtct
      301 ctttcccatc gcccaacgtg tgattattgt tctgcttcct gggtagaagt ccctaacgag
      361 tcccctgttg gcctgggtga gtctcctcaa caagcttctt ttctgagcag gaacaccttt
      421 ctaatgtgga cattgcagga caatcgctcg cgaatcctaa gtgcatgtga ccccaccttc
      481 cagcagcaga ggacgtttct cctcgctcca gagtgcttgg aatatcttgg tggcaccttc
      541 tgttaccagt gacaacctgt tgacactaag aggtctggac aggatttccc gtcaccgcag
      601 ccataccacc tattacatct cgattttctg tgacttatgc gctccggtct ctgcacgcct
      661 gcagaggcat tggagatgcc ttctagcaca gaggcggcga ccgatgaatg tgacgatgcg
      721 gagctccggt gccgggtagc cgtggaggag ctgagtcctg gagggcaacc tcgcaagcgc
      781 caggccctgc gcgccgcaga gctgagccta ggtcgaaacg aacgacgtga gttaatgctg
      841 cgactgcagg caccgggacc cacggggcgg ccacgctgtt tcccgctacg cgccgtgcgc
      901 ctcttcaccc gcttcgctgc gactgggcgc agcacgttgc ggctccccac cgatggagtc
      961 cctggagctg gctcagtgca actgctcctc tccgactgtc ccccggagcg cttgcgccgc
     1021 ttcctgcgca cgctgcgcct gaagctggcg gttgcccctg ggccgggacc cgcctctgcc
     1081 cgcgcacagt tgctcggccc gcggccccga gactttgtca ccatcagtcc agtgcagcca
     1141 gaggaactgc agcgtgctgc agccaccaag gctccagatt ctgcgctgga aaagcggcca
     1201 atggaatccc agactagtac ggaagctcca aggtggcccc tgcctgtgaa gaagctgcgc
     1261 atgccctcca ccaaaccgaa gctttctgaa gagcaggccg ctgtgctgag gatggttctg
     1321 aaaggccaga gcattttctt cactgggagc gcagggacag gaaagtccta cctgctgaaa
     1381 catatcctgg gttccctgcc ccctactggt actgtggcca ctgccagcac tggggtggca
     1441 gcctgccaca ttgggggcac cacccttcat gcctttgcag gcatcggctc aggccaggct
     1501 cccctggccc agtgcatggc cctggccaat cggccaggtg tgcggcaggg ctggctgaac
     1561 tgccaacgtt tggtcattga cgagatctcc atggtggagg cagacttctt tgacaagttg
     1621 gaagctgtgg ccagagctgt ccggcaacag aagaagccat ttggagggat ccagctcatc
     1681 atctgtgggg acttcctaca gttgccacca gtgaccaaag gctcccagca gcctcagttc
     1741 tgctttcagg ccaagagctg gaggaggtgt gtgcctgtga ttctggagct gactgaggtg
     1801 tggaggcaag cagaccagac cttcatctct ctactgcagg ctgtgaggtt aggcagatgt
     1861 tcagatgaag taacccgcca gctcagggcc acagctgccc ataaggtggg acgagatgga
     1921 attgtagcca cgagactatg tacccatcag gatgatgtgg ccctgaccaa cgagaagtgg
     1981 ctgaaggcac tgccaggtga tgtacacagc tttgaggcta tagacagtga ccctgagcta
     2041 agccggaccc tggatgctca gtgccctgtt agccgtgtcc ttcagttaaa gctgggggct
     2101 caggtcatgc tggtgaagaa cttggcagtg tctcggggcc tggtgaacgg tgcccgaggg
     2161 gtggtagttg ggtttgagtc cgaagggaga gggctccccc gggtacggtt cctgtgtggt
     2221 atcactgagg tcatccgcac tgaccgctgg acagtacagg tcactggggg acagtacctc
     2281 agccggcagc agcttcccct acagctggcc tgggccatat ccatccacaa aagccagggc
     2341 atgtctctgg actgtgtgga gatctctctg ggccgtgtgt ttgccagtgg tcaagcctat
     2401 gtggccctct cccgggcccg tagcctccag ggtcttcgtg tgctggactt tgaccccacg
     2461 gtggttcgat gtgactcccg agtgctgcat ttctatgcca ccctgcggca gggcaggggc
     2521 ctcagtctgg agtcccaaga cgatgaggag gcaaactcag atctggagaa catggaccca
     2581 aacctctgac ctcagctgaa agagaagaca aacttttagc tttttttcct gggtcaaggc
     2641 cctaggaatt aactggggag aggcctgtgt ttcttccctt attcagcctc tggtagggtt
     2701 aagggacaca gtttcccatc tacttaacta gcattgcctc agtttcacct atttccccgg
     2761 ggaaatgact tccagggttc aaagctagaa atggtgatgg ttaccagagg acaaagctct
     2821 ctaccaaggg tggaacacac agccacagag ttctttgcag gctggagagg cagtgcgggc
     2881 aggggctgca ttcagcagca gcagcagtag gagcagcctg tcttattaca ccgcatgtat
     2941 ttattttgtg tgcttgtgca cgcacagcat attgtacatg tgaaggtcag aggacaactc
     3001 gaggaagttg gttttctctt tccccaagtg tgttctgggg gttaaattca ggtcacaggg
     3061 cttggtagca ggcacttata cccgatgagc aatcttgcta ccagggtcgg ttctaatttt
     3121 ctttgtgtta ttataacaaa atatataagg ctgagtactt tatgaaaaaa atgatttatt
     3181 tttaattaat atatgctcac agttctagaa gatgaagaac acggaaccag cctcgtctca
     3241 gctttgctgg ggtttgacgg tagcagcaac cgcctggcgg ggacacttgc aggcaggatc
     3301 atgagagaca ggcacagagg atggtgatgc tggggaagag tattaatccg tccatgagga
     3361 caggaccccc ttgctttagt tacctcccat gcaatccatc tctgaaaggt tacatcatct
     3421 taacactgct acgctaggga ctaagcttcc agtacataaa cctataaggg aaaccatcca
     3481 aactatggca ggagcctaga ggggattcag gccagacaca agcccaagat agaagtttaa
     3541 ttaccttcac agctgtgctc agcctagcac agccccaagt aaacatcatt cagagcccga
     3601 ctgagaacag acgctgcaaa atgtgctggg tttaggggag aggccgtgtt taggatacgg
     3661 agatgtatgt tctcctttgt atttatttaa gccaaataaa actgtgaacc gtgtaa
//
