LOCUS       NM_001357349            8219 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus piezo-type mechanosensitive ion channel component 1
            (Piezo1), transcript variant 1, mRNA.
ACCESSION   NM_001357349 XM_006530955
VERSION     NM_001357349.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Xie,D., Ma,Y., Gao,C. and Pan,S.
  TITLE     Piezo1 activation on microglial cells exacerbates demyelination in
            sepsis by influencing the CCL25/GRP78 pathway
  JOURNAL   Int Immunopharmacol 142 (Pt A), 113045 (2024)
   PUBMED   39236454
  REMARK    GeneRIF: Piezo1 activation on microglial cells exacerbates
            demyelination in sepsis by influencing the CCL25/GRP78 pathway.
REFERENCE   2  (bases 1 to 8219)
  AUTHORS   Zhang,T., Bi,C., Li,Y., Zhao,L., Cui,Y., Ouyang,K. and Xiao,B.
  TITLE     Phosphorylation of Piezo1 at a single residue, serine-1612,
            regulates its mechanosensitivity and in vivo mechanotransduction
            function
  JOURNAL   Neuron 112 (21), 3618-3633 (2024)
   PUBMED   39270653
REFERENCE   3  (bases 1 to 8219)
  AUTHORS   Lee,P.R., Ha,T., Choi,H.S., Lee,S.E., Kim,C. and Hong,G.S.
  TITLE     Piezo1 mediates mechanical signals in TRPV1-positive nociceptors in
            mice
  JOURNAL   Acta Physiol (Oxf) 240 (11), e14236 (2024)
   PUBMED   39324481
  REMARK    GeneRIF: Piezo1 mediates mechanical signals in TRPV1-positive
            nociceptors in mice.
REFERENCE   4  (bases 1 to 8219)
  AUTHORS   Ezzo,M., Spindler,K., Wang,J.B., Lee,D., Pecoraro,G., Cowen,J.,
            Pakshir,P. and Hinz,B.
  TITLE     Acute contact with profibrotic macrophages mechanically activates
            fibroblasts via alphavbeta3 integrin-mediated engagement of Piezo1
  JOURNAL   Sci Adv 10 (43), eadp4726 (2024)
   PUBMED   39441936
  REMARK    GeneRIF: Acute contact with profibrotic macrophages mechanically
            activates fibroblasts via alphavbeta3 integrin-mediated engagement
            of Piezo1.
REFERENCE   5  (bases 1 to 8219)
  AUTHORS   Zhang,J., Zhao,Y., Wu,S., Han,M., Gao,L., Yang,K., Chen,H., Wang,C.
            and Xu,G.
  TITLE     Mechanosensing by Piezo1 in gastric ghrelin cells contributes to
            hepatic lipid homeostasis in mice
  JOURNAL   Sci Signal 17 (859), eadq9463 (2024)
   PUBMED   39436995
  REMARK    GeneRIF: Mechanosensing by Piezo1 in gastric ghrelin cells
            contributes to hepatic lipid homeostasis in mice.
REFERENCE   6  (bases 1 to 8219)
  AUTHORS   Huang,H., Bae,C., Sachs,F. and Suchyna,T.M.
  TITLE     Caveolae regulation of mechanosensitive channel function in
            myotubes
  JOURNAL   PLoS One 8 (8), e72894 (2013)
   PUBMED   24023653
  REMARK    GeneRIF: perturbations of developing myotubes were investigated to
            see whether caveolae are functionally linked to MSCs.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 8219)
  AUTHORS   Andolfo,I., Alper,S.L., De Franceschi,L., Auriemma,C., Russo,R., De
            Falco,L., Vallefuoco,F., Esposito,M.R., Vandorpe,D.H.,
            Shmukler,B.E., Narayan,R., Montanaro,D., D'Armiento,M., Vetro,A.,
            Limongelli,I., Zuffardi,O., Glader,B.E., Schrier,S.L., Brugnara,C.,
            Stewart,G.W., Delaunay,J. and Iolascon,A.
  TITLE     Multiple clinical forms of dehydrated hereditary stomatocytosis
            arise from mutations in PIEZO1
  JOURNAL   Blood 121 (19), 3925-3935 (2013)
   PUBMED   23479567
REFERENCE   8  (bases 1 to 8219)
  AUTHORS   Coste,B., Xiao,B., Santos,J.S., Syeda,R., Grandl,J., Spencer,K.S.,
            Kim,S.E., Schmidt,M., Mathur,J., Dubin,A.E., Montal,M. and
            Patapoutian,A.
  TITLE     Piezo proteins are pore-forming subunits of mechanically activated
            channels
  JOURNAL   Nature 483 (7388), 176-181 (2012)
   PUBMED   22343900
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 8219)
  AUTHORS   Coste,B., Mathur,J., Schmidt,M., Earley,T.J., Ranade,S.,
            Petrus,M.J., Dubin,A.E. and Patapoutian,A.
  TITLE     Piezo1 and Piezo2 are essential components of distinct mechanically
            activated cation channels
  JOURNAL   Science 330 (6000), 55-60 (2010)
   PUBMED   20813920
  REMARK    GeneRIF: overexpression of Piezo1 or Piezo2 induced 2 kinetically
            distinct mechanically activated (MA) currents; Piezos are expressed
            in several tissues; propose that Piezos are components of MA cation
            channels
REFERENCE   10 (bases 1 to 8219)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114917.2.
            
            On Oct 26, 2017 this sequence version replaced XM_006530955.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HQ215520.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-355               AC114917.2         149943-150297       c
            356-451             AC114917.2         112682-112777       c
            452-574             AC114917.2         106591-106713       c
            575-617             AC114917.2         105234-105276       c
            618-756             AC114917.2         104997-105135       c
            757-946             AC114917.2         101090-101279       c
            947-1160            AC114917.2         100734-100947       c
            1161-1332           AC114917.2         100474-100645       c
            1333-1419           AC114917.2         100298-100384       c
            1420-1507           AC114917.2         100130-100217       c
            1508-1608           AC114917.2         99172-99272         c
            1609-1866           AC114917.2         98596-98853         c
            1867-1978           AC114917.2         97628-97739         c
            1979-2157           AC114917.2         97361-97539         c
            2158-2306           AC114917.2         97119-97267         c
            2307-2489           AC114917.2         96774-96956         c
            2490-2602           AC114917.2         96420-96532         c
            2603-2763           AC114917.2         95708-95868         c
            2764-2940           AC114917.2         95371-95547         c
            2941-3066           AC114917.2         94702-94827         c
            3067-3267           AC114917.2         94385-94585         c
            3268-3472           AC114917.2         93758-93962         c
            3473-3577           AC114917.2         91575-91679         c
            3578-3731           AC114917.2         90627-90780         c
            3732-3975           AC114917.2         90283-90526         c
            3976-4072           AC114917.2         90087-90183         c
            4073-4244           AC114917.2         89823-89994         c
            4245-4334           AC114917.2         89207-89296         c
            4335-4435           AC114917.2         89031-89131         c
            4436-4507           AC114917.2         88523-88594         c
            4508-4608           AC114917.2         88067-88167         c
            4609-4702           AC114917.2         87429-87522         c
            4703-4759           AC114917.2         87302-87358         c
            4760-4932           AC114917.2         87043-87215         c
            4933-5039           AC114917.2         86806-86912         c
            5040-5246           AC114917.2         86443-86649         c
            5247-5505           AC114917.2         85978-86236         c
            5506-5694           AC114917.2         85723-85911         c
            5695-6007           AC114917.2         85275-85587         c
            6008-6140           AC114917.2         84792-84924         c
            6141-6288           AC114917.2         84558-84705         c
            6289-6503           AC114917.2         84225-84439         c
            6504-6662           AC114917.2         83930-84088         c
            6663-6810           AC114917.2         83697-83844         c
            6811-6999           AC114917.2         82454-82642         c
            7000-7092           AC114917.2         82287-82379         c
            7093-7265           AC114917.2         82025-82197         c
            7266-7388           AC114917.2         81837-81959         c
            7389-7468           AC114917.2         81617-81696         c
            7469-7685           AC114917.2         81278-81494         c
            7686-8219           AC114917.2         80666-81199         c
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 71.31 cM"
     gene            1..8219
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /note="piezo-type mechanosensitive ion channel component
                     1"
                     /db_xref="GeneID:234839"
                     /db_xref="MGI:MGI:3603204"
     exon            1..355
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     CDS             292..7935
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein PIEZO1; family with sequence similarity 38, member
                     A"
                     /codon_start=1
                     /product="piezo-type mechanosensitive ion channel
                     component 1 isoform 1"
                     /protein_id="NP_001344278.1"
                     /db_xref="CCDS:CCDS90474.1"
                     /db_xref="GeneID:234839"
                     /db_xref="MGI:MGI:3603204"
                     /translation="MEPHVLGAGLYWLLLPCTLLAASLLRFNALSLVYLLFLLLLPWL
                     PGPSRHSIPGHTGRLLRALLCLSLLFLVAHLAFQICLHTVPHLDQFLGQNGSLWVKVS
                     QHIGVTRLDLKDIFNTTRLVAPDLGVLLASSLCLGLCGRLTRKARQSRRTQELQDDDD
                     DDDDDDEDIDAAPAVGLKGAPALATKRRLWLASRFRVTAHWLLMTSGRTLVIVLLALA
                     GIAHPSAFSSVYLVVFLAICTWWSCHFPLSPLGFNTLCVMVSCFGAGHLICLYCYQTP
                     FIQDMLPPGNIWARLFGLKNFVDLPNYSSPNALVLNTKHAWPIYVSPGILLLLYYTAT
                     SLLKLHKSCPSELRKETPREDEEHELELDHLEPEPQARDATQGEMPMTTEPDLDNCTV
                     HVLTSQSPVRQRPVRPRLAELKEMSPLHGLGHLIMDQSYVCALIAMMVWSIMYHSWLT
                     FVLLLWACLIWTVRSRHQLAMLCSPCILLYGLTLCCLRYVWAMELPELPTTLGPVSLH
                     QLGLEHTRYPCLDLGAMLLYLLTFWLLLRQFVKEKLLKKQKVPAALLEVTVADTEPTQ
                     TQTLLRSLGELVTGIYVKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVY
                     YTLWRKLLRVFWWLVVAYTMLVLIAVYTFQFQDFPTYWRNLTGFTDEQLGDLGLEQFS
                     VSELFSSILIPGFFLLACILQLHYFHRPFMQLTDLEHVPPPGTRHPRWAHRQDAVSEA
                     PLLEHQEEEEVFREDGQSMDGPHQATQVPEGTASKWGLVADRLLDLAASFSAVLTRIQ
                     VFVRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVW
                     TCIIIVCKMLYQLKIVNPHEYSSNCTEPFPNNTNLQPLEINQSLLYRGPVDPANWFGV
                     RKGYPNLGYIQNHLQILLLLVFEAVVYRRQEHYRRQHQQAPLPAQAVCADGTRQRLDQ
                     DLLSCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAILTRRRREAIA
                     RLWPNYCLFLTLFLLYQYLLCLGMPPALCIDYPWRWSKAIPMNSALIKWLYLPDFFRA
                     PNSTNLISDFLLLLCASQQWQVFSAERTEEWQRMAGINTDHLEPLRGEPNPIPNFIHC
                     RSYLDMLKVAVFRYLFWLVLVVVFVAGATRISIFGLGYLLACFYLLLFGTTLLQKDTR
                     AQLVLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSNFCWVIQLFSLVCTVKGYYDPK
                     EMMTRDRDCLLPVEEAGIIWDSICFFFLLLQRRIFLSHYFLHVSADLKATALQASRGF
                     ALYNAANLKSINFHRQIEEKSLAQLKRQMKRIRAKQEKYRQSQASRGQLQSKDPQDPS
                     QEPGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEALPEDPRPAAQS
                     AFQMAYQAWVTNAQTVLRQRRERARQERAEQLASGGDLNPDVEPVDVPEDEMAGRSHM
                     MQRVLSTMQFLWVLGQATVDGLTRWLRAFTKHHRTMSDVLCAERYLLTQELLRVGEVR
                     RGVLDQLYVGEDEATLSGPMETRDGPSTASSGLGAEEPLSSMTDDTSSPLSTGYNTRS
                     GSEEIVTDAGDLQAGTSLHGSQELLANARTRMRTASELLLDRRLHIPELEEAERFEAQ
                     QGRTLRLLRAGYQCVAAHSELLCYFIIILNHMVTASAASLVLPVLVFLWAMLTIPRPS
                     KRFWMTAIVFTEVMVVTKYLFQFGFFPWNSYVVLRRYENKPYFPPRILGLEKTDSYIK
                     YDLVQLMALFFHRSQLLCYGLWDHEEDRYPKDHCRSSVKDREAKEEPEAKLESQSETG
                     TGHPKEPVLAGTPRDHIQGKGSIRSKDVIQDPPEDLKPRHTRHISIRFRRRKETPGPK
                     GTAVMETEHEEGEGKETTERKRPRHTQEKSKFRERMKAAGRRLQSFCVSLAQSFYQPL
                     QRFFHDILHTKYRAATDVYALMFLADIVDIIIIIFGFWAFGKHSAATDIASSLSDDQV
                     PQAFLFMLLVQFGTMVIDRALYLRKTVLGKLAFQVVLVVAIHIWMFFILPAVTERMFS
                     QNAVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLV
                     ELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYG
                     MGGLIILFLIAIIWFPLLFMSLIRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSI
                     VPFTPQAYEELSQQFDPYPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKQ
                     ELYNGTADITLRFTWNFQRDLAKGGTVEYTNEKHTLELAPNSTARRQLAQLLEGRPDQ
                     SVVIPHLFPKYIRAPNGPEANPVKQLQPDEEEDYLGVRIQLRREQVGTGASGEQAGTK
                     ASDFLEWWVIELQDCKADCNLLPMVIFSDKVSPPSLGFLAGYGIVGLYVSIVLVVGKF
                     VRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPET
                     MIKWTRERE"
     exon            356..451
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            452..574
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            575..617
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            618..756
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            757..946
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1160
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1332
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1419
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..1507
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1608
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1866
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            1867..1978
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2157
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2306
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2489
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            2490..2602
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            2603..2763
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            2764..2940
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            2941..3066
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            3067..3267
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            3268..3472
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            3473..3577
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            3578..3731
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            3732..3975
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            3976..4072
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            4073..4244
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            4245..4334
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            4335..4435
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            4436..4507
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            4508..4608
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            4609..4702
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            4703..4759
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            4760..4932
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            4933..5039
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            5040..5246
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            5247..5505
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            5506..5694
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            5695..6007
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            6008..6140
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            6141..6288
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            6289..6503
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            6504..6662
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            6663..6810
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            6811..6999
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            7000..7092
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            7093..7265
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            7266..7388
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            7389..7468
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            7469..7685
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     exon            7686..8219
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8177..8182
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /note="hexamer: AGTAAA"
     polyA_site      8201
                     /gene="Piezo1"
                     /gene_synonym="9630020g22; Fam38a; mKIAA0233"
                     /note="major polyA site"
ORIGIN      
        1 gggagagagc cgccgcgtcg ccgtccggcc cagctcggcc tccacgagcc gccgtgagtt
       61 ccgagccggg cgggctgaca acccagcgtg cgcgggatcc ggccgtcggg aaccagaggg
      121 cgcggccggg ccggccggcg acccgagatc gaggccggcg ggaggtcaca gcagccgcgc
      181 gccgcacgtg cggagacccg cccagagtgc catggcgcgc ggttcctccg gccctcgcac
      241 ctccctttag ccacggcggc gcgaccccgg gcggtccccc ggccgcgggg catggagccg
      301 cacgtgctgg gcgccgggct ctactggctg ttgctgccct gcacgctcct ggcggcctcc
      361 ctgttacgct tcaatgctct ctcgctggtc tatttgctgt ttctactgct gctgccctgg
      421 cttccaggcc cctcaagaca cagcatacca ggtcacacag gtcgcctgct ccgtgcacta
      481 ctctgcctca gcctcctctt cctggtggcc caccttgcct ttcagatatg cctacacacc
      541 gtgcctcacc tggaccagtt tctgggacaa aacggtagcc tttgggtgaa ggtgtctcaa
      601 cacatagggg ttacgaggct ggacctgaag gacatcttta acaccaccag gctggtagca
      661 cctgacctgg gagtgctgct ggcgtcctcc ctttgccttg gcctctgtgg acgcctcacg
      721 aggaaagccc ggcagagtcg gcgcacccag gagctgcagg atgatgacga tgacgatgat
      781 gacgacgatg aagacataga tgctgcccca gccgtggggc tgaagggagc ccctgccctg
      841 gcaaccaaac gcaggctgtg gctggcctcc cgcttccggg tcacggccca ctggctgctg
      901 atgacctctg gacggacgct ggtcattgtg ttgctggccc tggcaggcat agcccaccct
      961 tcggccttct ccagcgtcta cctggtggtg ttcctggcca tctgcacctg gtggtcctgt
     1021 cactttcctc tcagccccct gggctttaac accctctgtg tcatggtgag ctgttttggt
     1081 gccggccatc tcatttgcct atactgctat cagacaccat ttatccagga catgttaccc
     1141 cctgggaaca tctgggccag gctatttggt ctcaagaact tcgtagacct ccctaactac
     1201 tccagcccca acgccctggt actcaacact aagcacgcct ggcccatcta tgtgagtccc
     1261 ggaatcctgc tgctgctata ttacacagcc acctctctcc tgaagctcca caagagctgt
     1321 ccctcagagc tgaggaagga gacacccagg gaggatgagg agcatgagct ggaactggac
     1381 cacctggagc cagagcccca ggctagggac gccacccagg gtgagatgcc catgaccacg
     1441 gaacctgacc ttgacaactg caccgtgcat gtactaacca gccagagccc tgtccgccag
     1501 cgtccagttc gccccaggct ggctgagctg aaagagatgt caccgctaca tggcctgggc
     1561 cacctcatca tggaccagag ctatgtgtgt gccctgattg ccatgatggt gtggagcatc
     1621 atgtaccaca gctggctgac cttcgtcctg ctgctctggg cctgcctcat ctggacggtg
     1681 cgtagccgcc accagctggc tatgctctgc tcgccctgca tcctgctgta tgggctgacg
     1741 ctctgctgcc tgaggtacgt gtgggccatg gaacttcctg agctgcccac caccctgggc
     1801 cctgtcagcc tgcaccagtt gggactggaa cacacacgct acccttgcct ggacctcggt
     1861 gccatgctcc tctatctgct cacattctgg ctccttctgc gtcagttcgt gaaggagaag
     1921 ctgctgaaga agcaaaaggt gcctgcggcg cttttggagg tcacggtggc cgacactgag
     1981 cccacacaga cccagacgct gctgcggagc ctgggggagc tggtcaccgg catctacgtc
     2041 aaatactgga tctatgtgtg cgccggcatg ttcattgtgg tcagcttcgc cggccgcctg
     2101 gtggtctaca aaatcgtcta catgttcctc ttcctgctgt gcctcacctt gttccaggtc
     2161 tactacaccc tgtggaggaa gctgctgcgt gtcttctggt ggctcgtggt ggcctataca
     2221 atgctcgtgc tcatcgctgt gtacaccttc cagttccagg acttccccac ctattggcgc
     2281 aacctcacgg gcttcacgga cgagcagttg ggcgacctgg gcctggagca gttcagtgtg
     2341 tcggagctct tttccagtat cctcatccct ggcttcttcc tgctggcctg catcctgcag
     2401 ctgcactact tccacagacc gttcatgcag ctcactgacc tggagcacgt gccgccacca
     2461 ggcacccgcc accctcgatg ggctcacagg caggatgcag tgagcgaggc ccctctgctt
     2521 gagcatcagg aggaagagga agtcttcagg gaagatgggc agagcatgga tgggccccac
     2581 caggccacac aggtccctga gggtacggcc agcaagtggg gcctggtggc tgaccggctg
     2641 ctggacctag cggccagctt ctcggctgtc ctcacccgaa tccaggtgtt cgttcggcgc
     2701 ttgctagaac ttcacgtctt caagctggtg gccctctaca ctgtctgggt ggccctgaaa
     2761 gaagtgtctg tgatgaacct gctgctggtg gtgctatggg ccttcgcctt gccctatccg
     2821 cgcttccggc ccatggcttc ctgcctgtcc accgtgtgga cctgtatcat cattgtatgc
     2881 aagatgctct atcagctcaa gattgtcaac ccgcatgagt actccagcaa ctgcactgag
     2941 cccttcccca acaataccaa cttgcagcct ttggagatca accagtcttt gctgtaccgt
     3001 ggccctgttg accctgccaa ctggtttggg gtgcggaagg gttaccccaa cttgggctat
     3061 atccagaacc acctgcagat ccttctgttg ctggtgtttg aggccgtggt gtaccggcgc
     3121 caagagcact accgccggca gcaccagcag gcccctctgc ccgcccaggc tgtgtgcgca
     3181 gatggcaccc gccagaggtt ggaccaggac ctacttagct gcctcaagta tttcatcaac
     3241 ttcttcttct acaaattcgg gctggagatc tgcttcttga tggccgtgaa tgtgattggg
     3301 cagcgtatga acttcatggt gatcttacac ggttgctggt tggtggccat ccttacacgc
     3361 cggcgccgtg aggccatcgc ccgcctctgg cctaactact gtctgttcct cacgctgttc
     3421 ctgctgtacc agtacctgct gtgtttgggc atgccccctg cactgtgcat tgactatcca
     3481 tggcgctgga gcaaggccat ccccatgaat tccgccctca tcaagtggct gtacctacct
     3541 gacttcttca gagcccccaa ctccaccaac cttatcagtg acttcctcct gctgctttgc
     3601 gcctcccagc agtggcaggt cttctcagcg gagcgaacgg aggagtggca acgcatggcg
     3661 ggcatcaaca ctgaccacct ggagcccctg cgtggggagc ccaaccctat acccaacttc
     3721 atccactgca ggtcctatct ggatatgctg aaggtggccg tcttccgcta cctgttctgg
     3781 ctggtgctcg ttgtggtgtt tgttgcgggg gccacccgca taagcatctt cgggctgggg
     3841 tacctgctag cctgcttcta cctgctgctg tttggcacta ccctgctgca gaaggacacg
     3901 cgagcccagc tcgtgctgtg ggactgcctc atcctctata atgtcactgt catcatctct
     3961 aagaatatgc tgtcgctcct gtcctgtgtc ttcgtggagc aaatgcagag caacttctgc
     4021 tgggtcatcc agctcttcag cctcgtgtgc acagtcaaag gctactatga tcccaaagag
     4081 atgatgacca gggaccggga ctgcctgctg cctgtggagg aggccgggat catctgggac
     4141 agtatctgct tcttcttcct gctcttgcaa cggcgcatct ttctcagcca ctacttcctg
     4201 catgtcagcg ctgacctgaa agccacagcc ctgcaggcat ccaggggctt tgccctctac
     4261 aatgcagcca acctgaagag catcaacttc catcgccaga ttgaggagaa gtccctggcc
     4321 cagctgaaaa gacagatgaa gcgcatccgt gccaaacagg agaagtacag gcagagccag
     4381 gcaagtcgtg gccaactcca gtccaaagac cctcaggatc ccagccagga gccagggcct
     4441 gacagcccag ggggctcctc cccgccacgg agacagtggt ggcgcccctg gctggaccac
     4501 gccacagtca tccactctgg cgactacttc ctgtttgagt cagatagcga ggaggaagag
     4561 gaggccctac ctgaggaccc caggcctgca gctcagagtg ccttccagat ggcataccag
     4621 gcatgggtaa ccaatgccca gacagtgctg aggcagcgtc gggagcgggc acggcaggag
     4681 cgggcagagc agctggcttc tggaggtgac ttgaacccag atgtggaacc agtagatgtc
     4741 ccagaagatg agatggcagg ccgtagccac atgatgcagc gtgtgctaag caccatgcag
     4801 ttcctgtggg tgctgggcca ggccacggta gacgggctga cgcgctggct gcgtgcattc
     4861 acgaagcacc accgcaccat gagcgatgtg ctgtgcgcag agcgctacct gctcacccag
     4921 gagcttcttc gggttggaga ggtacgccga ggtgtgctgg accagcttta tgtgggtgaa
     4981 gatgaggcca cattgtcagg tcccatggag acccgggatg gacccagcac agcctcaagt
     5041 gggctgggag ccgaagagcc tttgagtagc atgacagacg acaccagcag ccccctgagc
     5101 acaggctata acacccgcag tggcagtgag gagattgtca ccgacgctgg ggacctccag
     5161 gctgggacct ccctgcacgg ctcccaagag cttttagcca atgctcgtac ccggatgcgc
     5221 acggccagcg agctgctact ggataggcgc ctgcatatcc ctgagctgga ggaggccgag
     5281 cggtttgagg cacagcaggg ccggactctg cggctgctca gggctgggta ccagtgcgtg
     5341 gcggcacact cggagctgct ctgttacttc atcatcatcc ttaaccacat ggtgacagcc
     5401 tcggctgcct ccctggtgct gcccgtgctt gtgttcctgt gggccatgct gaccatcccg
     5461 aggcctagca agcgcttttg gatgacagct atcgtcttca ctgaggtcat ggtggtcacc
     5521 aaatacctgt tccagttcgg cttcttcccc tggaacagct acgttgtgct gcggcgctat
     5581 gagaacaagc cctacttccc tccgcgaatc ctgggccttg agaaaacgga cagctacatc
     5641 aagtatgacc tggtgcagct catggccctc ttcttccacc gctcgcagct actgtgttat
     5701 ggcctctggg accatgagga ggatcgctat cccaaggacc attgcaggag tagtgtgaag
     5761 gaccgggagg ccaaggaaga gccagaagct aagctggaat cgcagtctga gacaggcact
     5821 gggcatccca aggagccagt gttggccggt actcccaggg accacatcca agggaaagga
     5881 agtattagat ccaaggatgt tatccaagat cccccagagg accttaagcc ccggcacacg
     5941 aggcacatca gcatacgctt caggaggcgc aaggagactc caggacccaa aggaacagca
     6001 gtcatggaga ctgagcacga ggagggagaa ggaaaagaaa ctacagagag aaagaggccg
     6061 cgtcacactc aagaaaaatc gaagtttcgg gagagaatga aggcagctgg gcgccggctg
     6121 cagagcttct gtgtgtcact ggcccagagc ttctaccaac ccttgcagcg tttcttccat
     6181 gacattctgc acacaaagta ccgggcggcc accgacgtct acgccctcat gttcctggcc
     6241 gatattgtcg acatcatcat catcatcttt ggtttttggg cttttgggaa gcactctgca
     6301 gccacagaca ttgcatcctc gctgtcagat gaccaggtgc cacaggcctt cctgttcatg
     6361 ctgctggtcc agtttggcac catggtcatc gaccgtgccc tctacctgcg caagactgtc
     6421 ctgggaaagc tggcctttca ggtggtcctg gtggtggcga ttcacatctg gatgttcttt
     6481 atcttaccgg ctgtcactga gaggatgttc agccagaatg cggtggcaca gctgtggtac
     6541 ttcgtcaagt gcatttactt tgccctgtcc gcctaccaga tccgctgtgg ctaccccacc
     6601 cgtatcttgg gcaacttcct caccaagaaa tacaaccatc taaacctctt ccttttccag
     6661 gggttccgtc tagtgccgtt cctggtggag ctgcgggccg tcatggactg ggtgtggacc
     6721 gacaccacgc tgtccctgtc caactggatg tgtgtggaag acatctatgc caacatcttt
     6781 atcatcaagt gcagccgaga gacagagaag aaataccccc agcccaaggg gcagaagaag
     6841 aagaaaattg tcaagtatgg tatgggaggc ctcattatcc tcttcctcat cgccatcatc
     6901 tggttccctc tgctcttcat gtcactgatc cgctctgtgg tcggggtcgt caaccagccc
     6961 attgatgtca ccgtcaccct caagctaggc ggctatgagc cactgttcac catgagcgcc
     7021 cagcagccat ccattgtgcc attcacaccc caggcctacg aggagctgtc ccagcagttt
     7081 gacccctatc cactagccat gcagttcatt agccagtaca gtcctgagga catcgtcact
     7141 gcacagatcg agggcagctc gggggcgctg tggcgcatca gcccacccag ccgagcccag
     7201 atgaagcagg agctgtacaa cggcacagcc gacattacac tgcgctttac ctggaatttc
     7261 caaagggacc tggccaaggg tggcactgtg gagtatacta atgagaagca caccttggag
     7321 ctggccccca acagtacggc acgaaggcag ctggcccaac tgctcgaggg cagacctgac
     7381 cagtcagtgg tcattcccca tctcttcccc aagtacattc gtgctcccaa tgggcctgaa
     7441 gccaaccctg tgaagcagct gcagccagat gaggaagagg actaccttgg tgtgcgcatc
     7501 cagctgcgga gggagcaagt gggcacaggg gcctctgggg agcaagcggg caccaaggcc
     7561 tccgacttcc tcgagtggtg ggtcatcgag ctgcaggact gcaaggctga ctgcaacctg
     7621 ctgcccatgg tcatcttcag tgacaaggtc agcccaccta gcctgggctt cctggccggc
     7681 tacgggattg tggggctgta cgtctccatc gtgctggtgg ttggcaagtt tgtgcggggc
     7741 ttcttcagcg agatctctca ctccatcatg ttcgaggaac tgccgtgtgt ggaccgcatc
     7801 ctcaagctgt gccaggacat cttcttggtg cgcgagaccc gggagctgga gctggaggag
     7861 gagctatacg ccaagctcat cttcctgtac cgatctccag agaccatgat taagtggaca
     7921 cgtgagaggg agtaggagcc caggccctgg gcaccgggaa tgaaggagct gcctcagggc
     7981 agcatgacca ccagggctgg cagagctcct tgggttggag tgtagctgcc caaggcacga
     8041 gctgtgacag gtcctggcct acgtaagccc tgatgatgct gctgtcaaag ggacgctgtg
     8101 tccctaccca ttgcgtccct gccagttggt ctgggggcct ggctctccct tcccacaagt
     8161 actgtagggt tttctaagta aaacattttt atttatacaa acggactgtc acagaccaa
//
