LOCUS       NM_001198789             722 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus piercer of microtubule wall 2 (Pierce2), mRNA.
ACCESSION   NM_001198789 XR_105294 XR_108121
VERSION     NM_001198789.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 722)
  AUTHORS   Tai,L., Yin,G., Huang,X., Sun,F. and Zhu,Y.
  TITLE     In-cell structural insight into the stability of sperm microtubule
            doublet
  JOURNAL   Cell Discov 9 (1), 116 (2023)
   PUBMED   37989994
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 722)
  AUTHORS   Chen,Z., Shiozaki,M., Haas,K.M., Skinner,W.M., Zhao,S., Guo,C.,
            Polacco,B.J., Yu,Z., Krogan,N.J., Lishko,P.V., Kaake,R.M.,
            Vale,R.D. and Agard,D.A.
  TITLE     De novo protein identification in mammalian sperm using in situ
            cryoelectron tomography and AlphaFold2 docking
  JOURNAL   Cell 186 (23), 5041-5053 (2023)
   PUBMED   37865089
REFERENCE   4  (bases 1 to 722)
  AUTHORS   Zhou,L., Liu,H., Liu,S., Yang,X., Dong,Y., Pan,Y., Xiao,Z.,
            Zheng,B., Sun,Y., Huang,P., Zhang,X., Hu,J., Sun,R., Feng,S.,
            Zhu,Y., Liu,M., Gui,M. and Wu,J.
  TITLE     Structures of sperm flagellar doublet microtubules expand the
            genetic spectrum of male infertility
  JOURNAL   Cell 186 (13), 2897-2910 (2023)
   PUBMED   37295417
REFERENCE   5  (bases 1 to 722)
  AUTHORS   Gui,M., Farley,H., Anujan,P., Anderson,J.R., Maxwell,D.W.,
            Whitchurch,J.B., Botsch,J.J., Qiu,T., Meleppattu,S., Singh,S.K.,
            Zhang,Q., Thompson,J., Lucas,J.S., Bingle,C.D., Norris,D.P., Roy,S.
            and Brown,A.
  TITLE     De novo identification of mammalian ciliary motility proteins using
            cryo-EM
  JOURNAL   Cell 184 (23), 5791-5806 (2021)
   PUBMED   34715025
REFERENCE   6  (bases 1 to 722)
  AUTHORS   Tamplin,O.J., Cox,B.J. and Rossant,J.
  TITLE     Integrated microarray and ChIP analysis identifies multiple Foxa2
            dependent target genes in the notochord
  JOURNAL   Dev Biol 360 (2), 415-425 (2011)
   PUBMED   22008794
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158997.2, BC048630.1 and AW125236.1.
            
            On or before Nov 14, 2010 this sequence version replaced
            XR_105294.1, XR_108121.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CO039725.1, BY703996.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AC158997.2         127468-127484       c
            18-703              BC048630.1         1-686
            704-722             AW125236.1         1-19                c
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 40.08 cM"
     gene            1..722
                     /gene="Pierce2"
                     /gene_synonym="Ccpg1os; EG546143; Gm5918"
                     /note="piercer of microtubule wall 2"
                     /db_xref="GeneID:546143"
                     /db_xref="MGI:MGI:3648770"
     exon            1..236
                     /gene="Pierce2"
                     /gene_synonym="Ccpg1os; EG546143; Gm5918"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..578
                     /gene="Pierce2"
                     /gene_synonym="Ccpg1os; EG546143; Gm5918"
                     /note="cell cycle progression 1, opposite strand;
                     uncharacterized protein C15orf65 homolog"
                     /codon_start=1
                     /product="piercer of microtubule wall 2 protein"
                     /protein_id="NP_001185718.1"
                     /db_xref="CCDS:CCDS72276.1"
                     /db_xref="GeneID:546143"
                     /db_xref="MGI:MGI:3648770"
                     /translation="MARETDCDLDKKTSLTSDAEMRPEPPALCVNPGNPVFSCMLDPK
                     TLHTATSLSKAQMIMYKTSASQYGAFSPRPFFFPCKFLPQEQAFTEHLKTTGFYQNNS
                     LNVGPDRTRTIDSPNYQHTL"
     exon            237..709
                     /gene="Pierce2"
                     /gene_synonym="Ccpg1os; EG546143; Gm5918"
                     /inference="alignment:Splign:2.1.0"
     regulatory      678..683
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pierce2"
                     /gene_synonym="Ccpg1os; EG546143; Gm5918"
                     /note="hexamer: AATAAA"
     polyA_site      698
                     /gene="Pierce2"
                     /gene_synonym="Ccpg1os; EG546143; Gm5918"
                     /note="major polyA site"
ORIGIN      
        1 cacgcctgca gccggttgcc acggagacgg gctgccacac tggaagacgg agggtttcca
       61 cccaccgtct tcctccccag aaattgtagt ttacgggact ccgatcgtct tgctgttgct
      121 ttccgtcggt tctgtaagaa gactgggccc tacgccttcg ccagacttct cgctatcagg
      181 aaagagttgc ttttgccgaa gagattaaga tggcgagaga gacagactgc gacttggata
      241 agaaaacttc cttgacttca gatgcagaaa tgagacctga accaccggct ctgtgcgtga
      301 accctggcaa ccctgtgttt tcctgtatgt tggatcccaa gacattgcac acagccacct
      361 cactgtccaa agcccagatg attatgtaca agaccagtgc cagccagtac ggggccttct
      421 cgcccaggcc cttcttcttt ccctgtaagt tcctaccaca agagcaagcg tttacagagc
      481 acctcaaaac aaccggcttt tatcaaaaca acagcctaaa tgttggtcct gaccgaacca
      541 gaaccatcga ttctcctaat tatcaacaca cactatgaaa atacatcctt ctgacaatgg
      601 aagagaaaat gtcctggggg tataagtttc taagtctaga acaactaatt ttgagcattt
      661 gaaatttaat aacttttaat aaagaagata caaatgcata ttctcaaaaa aaaaaaaaaa
      721 aa
//
