LOCUS       NM_008602               4968 bp    mRNA    linear   ROD 27-JUL-2024
DEFINITION  Mus musculus protein inhibitor of activated STAT 2 (Pias2),
            transcript variant 1, mRNA.
ACCESSION   NM_008602 NM_027527 XM_990156 XM_990181 XM_990215 XM_990245
            XM_990276 XM_990305 XM_990333
VERSION     NM_008602.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Wu,W., Arunagiri,V., Do-Umehara,H.C., Chen,C., Gu,S., Biswas,I.,
            Ridge,K.M., Budinger,G.R.S., Liu,S. and Liu,J.
  TITLE     Miz1 represses type I interferon production and limits viral
            clearance during influenza A virus infection
  JOURNAL   Sci Signal 17 (831), eadg7867 (2024)
   PUBMED   38593156
  REMARK    GeneRIF: Miz1 represses type I interferon production and limits
            viral clearance during influenza A virus infection.
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Zhang,L., Toboso-Navasa,A., Gunawan,A., Camara,A., Nakagawa,R.,
            Finsterbusch,K., Chakravarty,P., Newman,R., Zhang,Y., Eilers,M.,
            Wack,A., Tolar,P., Toellner,K.M. and Calado,D.P.
  TITLE     Regulation of BCR-mediated Ca2+ mobilization by MIZ1-TMBIM4
            safeguards IgG1+ GC B cell-positive selection
  JOURNAL   Sci Immunol 9 (94), eadk0092 (2024)
   PUBMED   38579014
  REMARK    GeneRIF: Regulation of BCR-mediated Ca[2+] mobilization by
            MIZ1-TMBIM4 safeguards IgG1[+] GC B cell-positive selection.
REFERENCE   3  (bases 1 to 4968)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 4968)
  AUTHORS   Do-Umehara,H.C., Chen,C., Zhang,Q., Schleimer,R.P., Budinger,G.R.S.
            and Liu,J.
  TITLE     Suppression of Allergic Asthma by Loss of Function of Miz1-mediated
            Th1 Skewing
  JOURNAL   Am J Respir Cell Mol Biol 67 (3), 346-359 (2022)
   PUBMED   35833903
  REMARK    GeneRIF: Suppression of Allergic Asthma by Loss of Function of
            Miz1-mediated Th1 Skewing.
REFERENCE   5  (bases 1 to 4968)
  AUTHORS   Mei,L., Qv,M., Bao,H., He,Q., Xu,Y., Zhang,Q., Shi,W., Ren,Q.,
            Yan,Z., Xu,C., Tang,C., Hussain,M., Zeng,L.H. and Wu,X.
  TITLE     SUMOylation activates large tumour suppressor 1 to maintain the
            tissue homeostasis during Hippo signalling
  JOURNAL   Oncogene 40 (35), 5357-5366 (2021)
   PUBMED   34267330
REFERENCE   6  (bases 1 to 4968)
  AUTHORS   Kotaja,N., Karvonen,U., Janne,O.A. and Palvimo,J.J.
  TITLE     PIAS proteins modulate transcription factors by functioning as
            SUMO-1 ligases
  JOURNAL   Mol Cell Biol 22 (14), 5222-5234 (2002)
   PUBMED   12077349
REFERENCE   7  (bases 1 to 4968)
  AUTHORS   Cho,S.Y., Jeon,J.W., Lee,S.H. and Park,S.S.
  TITLE     p67 isoform of mouse disabled 2 protein acts as a transcriptional
            activator during the differentiation of F9 cells
  JOURNAL   Biochem J 352 Pt 3 (Pt 3), 645-650 (2000)
   PUBMED   11104669
REFERENCE   8  (bases 1 to 4968)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   9  (bases 1 to 4968)
  AUTHORS   Moilanen,A.M., Karvonen,U., Poukka,H., Yan,W., Toppari,J.,
            Janne,O.A. and Palvimo,J.J.
  TITLE     A testis-specific androgen receptor coregulator that belongs to a
            novel family of nuclear proteins
  JOURNAL   J Biol Chem 274 (6), 3700-3704 (1999)
   PUBMED   9920921
REFERENCE   10 (bases 1 to 4968)
  AUTHORS   Wu,L., Wu,H., Ma,L., Sangiorgi,F., Wu,N., Bell,J.R., Lyons,G.E. and
            Maxson,R.
  TITLE     Miz1, a novel zinc finger transcription factor that interacts with
            Msx2 and enhances its affinity for DNA
  JOURNAL   Mech Dev 65 (1-2), 3-17 (1997)
   PUBMED   9256341
  REMARK    Erratum:[Mech Dev 1997 Dec;69(1-2):219]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK029716.1, AC157991.5 and AK032463.1.
            
            On Aug 13, 2009 this sequence version replaced NM_008602.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029716.1, AK078813.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-704               AK029716.1         2-705
            705-705             AC157991.5         156900-156900       c
            706-2249            AK029716.1         707-2250
            2250-4968           AK032463.1         685-3403
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 52.07 cM"
     gene            1..4968
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /note="protein inhibitor of activated STAT 2"
                     /db_xref="GeneID:17344"
                     /db_xref="MGI:MGI:1096566"
     exon            1..212
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     CDS             189..2054
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1; E3
                     SUMO-protein ligase PIAS2; DAB2-interacting protein;
                     msx-interacting zinc finger protein; protein inhibitor of
                     activated STAT x; androgen receptor-interacting protein 3;
                     Msx-interacting-zinc finger; RING-type E3 ubiquitin
                     transferase PIAS2"
                     /codon_start=1
                     /product="E3 SUMO-protein ligase PIAS2 isoform 1"
                     /protein_id="NP_032628.3"
                     /db_xref="CCDS:CCDS37866.1"
                     /db_xref="GeneID:17344"
                     /db_xref="MGI:MGI:1096566"
                     /translation="MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHL
                     LKSGCSPAVQIKIRELYRRRYPRTLEGLCDLSTIKSSVFSLDGSSSPVEPDLPVAGIH
                     SLPSTSITPHSPSSPVGSVLLQDTKPTFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVL
                     IKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAET
                     SCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQ
                     ISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSE
                     IATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKA
                     AYESLILDGLFMEILNDCSDVDEIKFQEDGSWCPMRPKKEAMKVTSQPCTKVESSSVF
                     SKPCSVTVASDASKKKIDVIDLTIESSSDEEEDPPAKRKCIFMSETQSSPTKGVLMYQ
                     PSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPVSSMSSDLPGLDFLSLIPVDPQYCPP
                     MFLDSLTSPLTASSTSVTTTSPHESSTHVSSSSSRSETGVITSSGRNIPDIISLD"
     exon            213..687
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            688..772
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            773..823
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            824..914
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1049
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1155
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1229
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1390
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1524
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1696
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1836
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1874
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..4968
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2357..2362
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /note="hexamer: AATAAA"
     polyA_site      2379
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /note="major polyA site"
     regulatory      4742..4747
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
                     /note="hexamer: AATAAA"
     polyA_site      4764
                     /gene="Pias2"
                     /gene_synonym="ARIP3; Dib; DIP; Miz1; PIASxalpha;
                     PIASxalpha6; PIASxb; PIASxbeta; SIZ2"
ORIGIN      
        1 gcgcaggtca cgtgaggggc ccggtggagg tggtggaggt gtcggcgcgg cggcggcggc
       61 ggcggcggcg gcggccgcgg cgtctagagc ggcgcccagt gcaggatgtt gcaggagacg
      121 gcggcggtgt cgttggcggc agcgggtgga ggagcggcga cggctgaggc gcctgcgggc
      181 gggagaaaat ggcggatttc gaggagttga ggaatatggt ttctagtttt agggtttctg
      241 agttacaagt gttactgggc tttgctggac ggaataaaag tgggcgcaag catgacctcc
      301 tgatgagggc gttgcattta ttgaagagtg gctgcagccc tgcggttcag attaaaattc
      361 gagaattata tagacgccga tacccacgga cacttgaagg actttgtgat ctttccacaa
      421 tcaaatcatc ggttttcagc ttggatggta gctcatcacc cgtagaacct gacttgcctg
      481 tggctgggat ccactcgttg ccttcgactt caattacacc tcattcaccg tcatctcctg
      541 ttggttctgt actgctgcaa gacactaagc ccacctttga gatgcagcag ccgtctccgc
      601 ccattcctcc tgtccatcct gatgtgcagt taaaaaacct gcctttctat gatgtccttg
      661 atgttctcat caagcccacg agtttagttc aaagcagtat tcagcggttt caagagaagt
      721 tttttatttt tgctttgaca cctcagcaag ttagagaaat atgtatttca agggactttt
      781 tgccgggtgg caggagagac tacacagtcc aagtccagct gcgactttgc ttggcagaga
      841 ccagttgccc tcaagaagat aactatccca atagtttgtg tataaaagtc aatgggaaac
      901 tctttccttt gcctggctat gcgccaccac ctaaaaatgg gattgaacag aagcgccctg
      961 gacgccccct gaacattacg tctttagtta ggttgtcttc agctgtgcca aatcagattt
     1021 ctatttcttg ggcatctgaa attggaaaga attactccat gtctgtgtat cttgtacgac
     1081 agcttacatc agccatgtta ttacagagat taaaaatgaa aggtattaga aatcctgatc
     1141 attccagagc actaattaaa gaaaaactta ctgcagaccc tgatagtgaa attgctacaa
     1201 ctagtcttcg agtgtccttg atgtgccctt taggaaaaat gaggctgaca atcccgtgcc
     1261 gtgcagtgac ttgtacacat ctgcagtgct ttgatgctgc cctctatctt cagatgaatg
     1321 agaaaaagcc cacctggatc tgtcctgttt gtgacaaaaa ggctgcctat gagagtctga
     1381 tactagatgg gctttttatg gaaattctca atgactgttc tgatgtggat gagatcaaat
     1441 tccaggaaga tggttcttgg tgtcccatga gacctaaaaa agaagctatg aaagtaacca
     1501 gccagccctg tacaaaagta gaaagttcaa gtgtctttag taaaccttgt tcagtgactg
     1561 tagccagtga tgcaagcaag aagaagatag atgttattga tctaacaata gagagctctt
     1621 ctgatgaaga ggaagaccct cccgccaaaa ggaaatgcat ctttatgtca gaaacacaaa
     1681 gcagtccaac caaaggggtt ctcatgtatc agccatcttc tgtaagggtg cccagtgtga
     1741 cttcagttga tcctgctgct attccacctt cattaacaga ctactcagta ccattccacc
     1801 acacgccagt gtcaagcatg tcatcagatt tgccaggttt ggattttctt tcccttattc
     1861 cagttgatcc ccagtactgt cctcctatgt ttttggatag tctcacctca cccttaacag
     1921 caagcagtac gtctgtcacc accaccagcc cccatgaaag cagtactcac gttagttcat
     1981 ccagcagcag gagtgagaca ggggtcataa ccagcagtgg gaggaacatt cctgacatca
     2041 tctctttgga ctaaaggcgg acatacttga ttctgggaat cattcatcaa aactctttct
     2101 tggatgttta gtccatggaa gctgattttt gcaacttaat atttattgaa atgtcaaatg
     2161 gattctttgc tctctgagag atgaacatcg atgacttcag caagaaccag atgacatgca
     2221 aggacttttc ttatgctgta catggagcat cagatctatc tcatccacaa cggtggcttg
     2281 tgggacagtt gatttcagtt tctacgttac tggatggatt ctacacacca gttaatcttt
     2341 tgttacaata aacagcaata aagttatgta aatagttaac ttttctaatt aaagatgtaa
     2401 tcactgatat agactaacaa caacttctgt ttaatccagt atgaaggaaa aatagaagaa
     2461 gtcttatttc ctgaatgaat actttctatg gagatttgtt ctattttgtt gaagtaatga
     2521 agtgtcaata actggccaga atttgggttc tacttttctt ctttaggttt tctgttcttc
     2581 atgatcccat caaagcaaat aatctttgta ctgtgcattt ttctcttgtc gtttacgctt
     2641 tgatgttgtt tgctgatatg tgcagagtaa agtgctggga cttggttgct ctttataatt
     2701 atgtttaaga atactacaca gatgttctga tataaagtgt tattttattt tgggttacta
     2761 tacattcttg tagaattttc tgcagacttg agactttttt tttcccccag aaagtcacat
     2821 cctttgagtg aaatatgctt gagttaagtt gtggctagaa tactggtagg attttcttca
     2881 cagtgttcaa aaatatttaa tgttggaaaa catcagaaca tacttagagg tggaaataac
     2941 tgaattcttc ctaagaattt aattgatttc atatatttta caggataatg tagtatacta
     3001 tcttggttgg aattcttccc attttaatgg cactaataca taaattatat ttaagatctc
     3061 tgatcataat tctcatcaat ataatttcag cagtgacttt caagtttaaa gaaaagtcag
     3121 tggtaaatga cagagggtgg tactaaatct cattttccta attttttttt taaaagaaat
     3181 cttttgtgag cacacacaca caccaaatat ttgaaatgaa ttgagcaaaa gtgcccaaag
     3241 tcccacttta aaacacacct ctcacgtggc agtgctgggc gtatttacca cttgcttcat
     3301 ttaggttcct taatttttag tttcctaaat ttattatttt cctgatgcct agtgatctct
     3361 acttttgcta tgtcgactta aagctttttt tttaagtggc tactcttaat ttctcaacta
     3421 ctaacataat ggctctactt ccacccaaac agggacatgt tccccactgc agcattggtg
     3481 gagctctagt gagctggctt ttccctggct gtggctggtg ttttattttc tcaaactttc
     3541 atggcctctg tgcacctttt gtacgtctgc ggagttgtcc acatgtttcc tactttgctt
     3601 tcagttgctc gtgtttgatg atcttccctt ttgatagggc tttcgagcca tcaaagatat
     3661 ggaaagcaaa gcaaacccct gcgatcacag tggttactgc attgtggccc ctggcttgct
     3721 tgaccttagc cagcccaggt aaaggtttct tcccctagga tgttctgcta ttgctgcttt
     3781 acatacagag ccagtttctg ttacccacgt aaccaggaaa aataacctgg cttctgtagt
     3841 gtggtgtgtt ataaagaagt gatgcagttg ccatcattct gtttacccag gagagtctcg
     3901 aggactcctc actaggggtc actgaagtcc catgagtact gaaggttgca gtgcttcctt
     3961 agctctcgta acatgcctct gcttttgtcg ttgtcctgca tctgtcactt tgcatgtcaa
     4021 cagttcatga aattacaaac gaagtcactt tagttgacta gcatattaaa gttgggattg
     4081 ctttgcagct gtctctgaag gcataccaag tattttcttt ctgcacttgg ctctgtattt
     4141 acatggttgt tgcaaagctc ttctttcctt aatgtcacgt tttatgaaga tcctgataag
     4201 cagagtctct gtaatgcaga tttctgttta actagataga ctagaattaa gagatcttcc
     4261 acctcgccat tatctgagat atctacaccc aaggagtggt ggctaatgct gtagtcactt
     4321 ggccaacctg cattttcatc cccaccccac ctccttttta aaaaaataca catagaccta
     4381 tgaacgggct ttcttgcttt acttaacgtt aggcagtata gctgctgcac agctctcact
     4441 agctagctta atacttgata tctgttctga gacaattgta aaggcaaatg aaataggtca
     4501 gataagttta gtttgttcta gaacactaat gaaaacatga tcataggtaa gggatagtga
     4561 ttgtaatccg ttggtcctaa ccttttacat cacacaagct tccattagag gagtagtagt
     4621 gaatttgaag ggaaggtgtt gaatttaaac agtcatgttg aatgaagtta agtcaataga
     4681 cttaaaatat tgacctaatg tgtcttcaat cagtcctgga cactcacctt tgacttttgc
     4741 gaataaagat attcattagt tgcatttgtg ttttttaagt aaggttggtt ttcataaagt
     4801 aatttttgtt gttgttttca gttatctttg cttgattatt taaaagaaaa aataagttct
     4861 ttttttagaa actttttttt tcttggaagt ttatctccag ttctatggaa aatcataatt
     4921 tatttcccac atttttttca tcttaaaaat aaaaatcagt gcccattt
//
