LOCUS       NM_001373955            6602 bp    mRNA    linear   ROD 18-JUN-2024
DEFINITION  Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka),
            transcript variant 1, mRNA.
ACCESSION   NM_001373955 XM_006522034
VERSION     NM_001373955.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Vong,K.I., Lee,S., Au,K.S., Crowley,T.B., Capra,V., Martino,J.,
            Haller,M., Araujo,C., Machado,H.R., George,R., Gerding,B.,
            James,K.N., Stanley,V., Jiang,N., Alu,K., Meave,N., Nidhiry,A.S.,
            Jiwani,F., Tang,I., Nisal,A., Jhamb,I., Patel,A., Patel,A.,
            McEvoy-Venneri,J., Barrows,C., Shen,C., Ha,Y.J., Howarth,R.,
            Strain,M., Ashley-Koch,A.E., Azam,M., Mumtaz,S., Bot,G.M.,
            Finnell,R.H., Kibar,Z., Marwan,A.I., Melikishvili,G., Meltzer,H.S.,
            Mutchinick,O.M., Stevenson,D.A., Mroczkowski,H.J., Ostrander,B.,
            Schindewolf,E., Moldenhauer,J., Zackai,E.H., Emanuel,B.S.,
            Garcia-Minaur,S., Nowakowska,B.A., Stevenson,R.E., Zaki,M.S.,
            Northrup,H., McNamara,H.K., Aldinger,K.A., Phelps,I.G., Deng,M.,
            Glass,I.A., Morrow,B., McDonald-McGinn,D.M., Sanna-Cherchi,S.,
            Lamb,D.J. and Gleeson,J.G.
  CONSRTM   Spina Bifida Sequencing Consortiumdouble dagger
  TITLE     Risk of meningomyelocele mediated by the common 22q11.2 deletion
  JOURNAL   Science 384 (6695), 584-590 (2024)
   PUBMED   38696583
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6602)
  AUTHORS   Gajardo,T., Bernard,M., Lo,M., Turck,E., Leveau,C., El-Daher,M.T.,
            Deslys,A., Panikulam,P., Menche,C., Kurowska,M., Le Lay,G.,
            Barbier,L., Moshous,D., Neven,B., Farin,H.F., Fischer,A.,
            Menasche,G., de Saint Basile,G., Vargas,P. and Sepulveda,F.E.
  TITLE     Actin dynamics regulation by TTC7A/PI4KIIIalpha limits DNA damage
            and cell death under confinement
  JOURNAL   J Allergy Clin Immunol 152 (4), 949-960 (2023)
   PUBMED   37390900
  REMARK    GeneRIF: Actin dynamics regulation by TTC7A/PI4KIIIalpha limits DNA
            damage and cell death under confinement.
REFERENCE   4  (bases 1 to 6602)
  AUTHORS   Alvarez-Prats,A., Bjelobaba,I., Aldworth,Z., Baba,T., Abebe,D.,
            Kim,Y.J., Stojilkovic,S.S., Stopfer,M. and Balla,T.
  TITLE     Schwann-Cell-Specific Deletion of Phosphatidylinositol 4-Kinase
            Alpha Causes Aberrant Myelination
  JOURNAL   Cell Rep 23 (10), 2881-2890 (2018)
   PUBMED   29874576
  REMARK    GeneRIF: These studies define a role for PI4KA in myelin formation
            primarily affecting metabolism of key phospholipids and the actin
            cytoskeleton.
REFERENCE   5  (bases 1 to 6602)
  AUTHORS   Ziyad,S., Riordan,J.D., Cavanaugh,A.M., Su,T., Hernandez,G.E.,
            Hilfenhaus,G., Morselli,M., Huynh,K., Wang,K., Chen,J.N.,
            Dupuy,A.J. and Iruela-Arispe,M.L.
  TITLE     A Forward Genetic Screen Targeting the Endothelium Reveals a
            Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and
            Erythropoiesis
  JOURNAL   Cell Rep 22 (5), 1211-1224 (2018)
   PUBMED   29386109
  REMARK    GeneRIF: Mutation of Pi4ka found to promote myeloid and erythroid
            dysfunction. Subsequent validation experiments showed that targeted
            inactivation of the Pi4ka catalytic domain or reduction in mRNA
            expression inhibited myeloid and erythroid cell differentiation in
            vitro and promoted anemia in vivo
REFERENCE   6  (bases 1 to 6602)
  AUTHORS   Vaillancourt,F.H., Brault,M., Pilote,L., Uyttersprot,N.,
            Gaillard,E.T., Stoltz,J.H., Knight,B.L., Pantages,L., McFarland,M.,
            Breitfelder,S., Chiu,T.T., Mahrouche,L., Faucher,A.M., Cartier,M.,
            Cordingley,M.G., Bethell,R.C., Jiang,H., White,P.W. and Kukolj,G.
  TITLE     Evaluation of phosphatidylinositol-4-kinase IIIalpha as a hepatitis
            C virus drug target
  JOURNAL   J Virol 86 (21), 11595-11607 (2012)
   PUBMED   22896614
REFERENCE   7  (bases 1 to 6602)
  AUTHORS   Wuttke,A., Sagetorp,J. and Tengholm,A.
  TITLE     Distinct plasma-membrane PtdIns(4)P and PtdIns(4,5)P2 dynamics in
            secretagogue-stimulated beta-cells
  JOURNAL   J Cell Sci 123 (Pt 9), 1492-1502 (2010)
   PUBMED   20375060
REFERENCE   8  (bases 1 to 6602)
  AUTHORS   Simons,J.P., Al-Shawi,R., Minogue,S., Waugh,M.G., Wiedemann,C.,
            Evangelou,S., Loesch,A., Sihra,T.S., King,R., Warner,T.T. and
            Hsuan,J.J.
  TITLE     Loss of phosphatidylinositol 4-kinase 2alpha activity causes late
            onset degeneration of spinal cord axons
  JOURNAL   Proc Natl Acad Sci U S A 106 (28), 11535-11539 (2009)
   PUBMED   19581584
  REMARK    GeneRIF: Loss of phosphatidylinositol 4-kinase 2alpha activity
            causes late onset degeneration of spinal cord axons.
REFERENCE   9  (bases 1 to 6602)
  AUTHORS   Thuret,S., Bhatt,L., O'Leary,D.D. and Simon,H.H.
  TITLE     Identification and developmental analysis of genes expressed by
            dopaminergic neurons of the substantia nigra pars compacta
  JOURNAL   Mol Cell Neurosci 25 (3), 394-405 (2004)
   PUBMED   15033168
REFERENCE   10 (bases 1 to 6602)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC110573.11 and KY499708.1.
            
            On Sep 5, 2019 this sequence version replaced XM_006522034.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: KY499708.1, SRR10223970.18117.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                AC110573.11        7464-7530
            68-6385             KY499708.1         1-6318
            6386-6602           AC110573.11        132761-132977
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 10.71 cM"
     gene            1..6602
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /note="phosphatidylinositol 4-kinase alpha"
                     /db_xref="GeneID:224020"
                     /db_xref="MGI:MGI:2448506"
     exon            1..232
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..6385
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /EC_number="2.7.1.67"
                     /note="isoform 1 is encoded by transcript variant 1;
                     PI4-kinase alpha; ptdIns-4-kinase alpha;
                     phosphatidylinositol 4-kinase, catalytic, alpha
                     polypeptide"
                     /codon_start=1
                     /product="phosphatidylinositol 4-kinase alpha isoform 1"
                     /protein_id="NP_001360884.1"
                     /db_xref="GeneID:224020"
                     /db_xref="MGI:MGI:2448506"
                     /translation="MAAAGARGGGGGGGGGGGGGSGSSSGSSTSRGFYFNTVLSLARS
                     LAVQRPASLEKVQKLLCMCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCVVPY
                     LLRLLRGLPKVYWVEESTARKGRGNLPVAESFSFCLVTLLSDVACRDPSLRDEILEAI
                     LQVLHVLLGMCQALEIQEKEYLCKYAIPCLIGISRSFGRYSNSEESLLSKLFPKVSPH
                     SLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGIPPP
                     SSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSISPLFNGITYKEFCIPLEMLRE
                     LLNLVKKIVEEPVLKSLDAIVAGVMEANPSADLYYTTFSDPLYLTMFKMLRDTLYYMK
                     DLPTSFVKEIHDFVLEQFNMSQGELQKILHDADRIHSEMSPLKLRCQANAACVDLMVW
                     AVKDEQGAENLCIKLSEKLQSKTSSKVIIAHLPLLICCLQGLGRLCERFPVVVHSVTP
                     SLRDFLVIPSPVLVKLYKYHSQYHTVAGSDIKISVTNEHSESTLNVLPGKKNQPSMYE
                     QLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALG
                     HIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLF
                     QQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHLVDELLMNLLELF
                     VQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLF
                     RDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKN
                     DTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRSTDPD
                     RFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQ
                     FLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHK
                     DQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLN
                     KHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLTERPACVKKDYSNFMASL
                     NLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLITALDHSHPQHYTQAMFKLTAMLI
                     SSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMT
                     VEQKFGLFSVETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQV
                     EIFSSLLQRSMSLNIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLR
                     EKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQD
                     TRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTL
                     LLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWS
                     ISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELS
                     HVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDK
                     MGYVREYILWAAAKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLEQLVEEITGSL
                     SGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPE
                     AIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLQCRSDAEDECFSQEADG
                     KKICWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIE
                     CIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQI
                     KDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPF
                     KWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAA
                     NFIMKVIQNCFLSNRSRTYDMIQYYQNDIPY"
     exon            233..349
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            350..443
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            444..532
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            533..605
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            606..865
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            866..932
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1081
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1147
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1244
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1436
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1537
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1667
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1800
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1896
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..2080
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2184
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2185..2353
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2354..2404
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2405..2513
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2514..2650
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2651..2784
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2785..2867
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2868..2938
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2939..3063
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..3145
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            3146..3229
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            3230..3319
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            3320..3439
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            3440..3532
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            3533..3689
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            3690..3817
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            3818..3976
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            3977..4128
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            4129..4236
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            4237..4364
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            4365..4484
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            4485..4564
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            4565..4758
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            4759..4849
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            4850..5017
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            5018..5071
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            5072..5192
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            5193..5322
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            5323..5393
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            5394..5466
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            5467..5537
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            5538..5647
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            5648..5752
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            5753..5878
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            5879..5999
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            6000..6159
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            6160..6249
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            6250..6333
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     exon            6334..6602
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6583..6588
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /note="hexamer: AATAAA"
     polyA_site      6602
                     /gene="Pi4ka"
                     /gene_synonym="PI4K-alpha; Pik4ca"
                     /note="major polyA site"
ORIGIN      
        1 agtaaggacc ggtggtggcg agcccagagc tgtgaaccca gccgcctgcg ccggggcgtg
       61 cgctgtgatg gcggctgctg gggcccgggg aggaggcggc ggtggcggcg gcggcggcgg
      121 cggcggctcg gggtccagct ctggctccag cacctctcga ggcttttatt tcaacacagt
      181 actttcactg gcccgctccc tggctgtgca gagaccagca tccttggaga aggttcaaaa
      241 gctgctttgc atgtgcccag tagattttca tggaatcttc caactagatg aaagacggag
      301 agatgcagtg attgcattgg gcatttttct cattgaatct gatcttcagc acaaagattg
      361 tgttgttcct tatcttcttc gacttctcag gggtcttcca aaagtgtatt gggtagaaga
      421 aagcacagct cggaaaggca gaggtaacct cccagttgca gagagcttca gcttctgcct
      481 ggtgaccttg ctgtctgatg tggcttgcag ggacccctca cttagggatg agattttaga
      541 ggcaattttg caggttttac atgtcctttt gggaatgtgc caggccttgg agattcaaga
      601 aaaagaatac ctttgcaagt atgcgatccc atgcctgata ggaatctcaa gatcttttgg
      661 acgttacagc aattcagaag agtctctcct ctcaaagctt ttccccaaag tttctcctca
      721 ttccctgaga atcccagaag agctagaagg tgtccgaagg cgttccttta atgactttcg
      781 ctccatcctg cccagcaacc tgctaactgt ctgtcaggag ggcacactga agaggaaaac
      841 cagcagtgtg tccagcatct ctcaggttag cccagaacgt ggcatacccc ctcccagctc
      901 ccctggagga tctgcatttc actattttga agcttcttgc ctacctgatg ggactgcctt
      961 ggagcctgaa tactacttct ctaccatcag ctccagtttt tctatctctc ccctgttcaa
     1021 tggtattacc tataaggagt tctgtattcc actggaaatg cttcgggaac tcttaaactt
     1081 ggtgaagaag attgttgagg agcctgttct caaatcctta gatgcaattg tagccggcgt
     1141 gatggaggcc aatcccagtg ctgatctgta ctatacaacc ttcagtgatc ccctgtacct
     1201 gaccatgttc aagatgcttc gagatactct gtactacatg aaagatctgc ccacttcctt
     1261 tgtgaaagag atccatgatt ttgtgttgga gcagttcaac atgagccaag gggagctcca
     1321 aaagattctg catgacgcag ataggatcca cagtgagatg agcccactca aactgcgctg
     1381 ccaagcaaac gctgcctgtg tggacctcat ggtgtgggct gtgaaggatg agcagggtgc
     1441 agaaaacctt tgcatcaagc tgtctgaaaa gctgcagtcc aagacatcca gtaaggtcat
     1501 cattgcccat ttgcccttgc tgatctgctg cctacagggt ctgggccgcc tgtgtgaaag
     1561 gttcccagtg gtggtccact cagtgacgcc atctttgaga gactttctgg taattccatc
     1621 cccagtgctg gtgaagctct ataagtacca cagtcagtac cacacagttg ctggcagtga
     1681 tataaaaatc agtgtcacca atgagcattc cgagtcaact ctgaatgtct tgccgggcaa
     1741 gaagaaccag ccatccatgt atgagcagct ccgagacatc gccattgaca acatctgcag
     1801 gtgcctgaag gccggcctca ccgtggaccc tgtgatcgtg gaggccttcc tggccagcct
     1861 ctccaaccgg ctctacattt cccaggagag tgacaaggat gctcacttga ttcctgacca
     1921 taccatccgt gccttgggac acattgcagt agctttgagg gacactccaa aggtcatgga
     1981 gccaattctg cagatcctgc agcagaaatt ttgccagccc ccctcacccc ttgatgtgct
     2041 catcatcgac cagctgggct gcctggtgat cactggaaat caatacattt atcaggaagt
     2101 gtggaatctc ttccagcaaa tcagtgtaaa ggccagttca gttgtgtatt cagccaccaa
     2161 agactataaa gaccatggct acaggcactg ctccctagca gtgatcaatg ccctggccaa
     2221 cattgcagcc aacattcagg aggagcatct tgtggacgag ctgttgatga acctgctgga
     2281 actgtttgtg cagctggggc tggaggggaa aagagccagt gagagggcca gtgaaaaggg
     2341 gcctgccctg aaggcttcca gcagtgcagg aaatttgggc gtgctcattc ctgtaatagc
     2401 tgtgctcacc cgacgattgc cacccatcaa agaagctaaa cctaggctac agaagctttt
     2461 ccgagatttc tggctctact ctgtgctaat gggattcgct gtggaaggct caggactctg
     2521 gccggaagag tggtatgagg gagtctgtga aatagctacc aagtcacctc tgcttacctt
     2581 tcccagcaaa gagcctctgc gatcagtcct ccagtacaat tcagctatga aaaacgatac
     2641 ggtcacccct gctgaactga gtgagctccg aagtaccatc atcaacttgc tagatcctcc
     2701 tcctgaggtg tctgccctca tcaacaagct ggactttgcc atgtctacct atctcttgtc
     2761 tgtgtatcgg ctggagtaca tgagggtact gcgttctaca gatccagatc gcttccaggt
     2821 aatgttctgc tactttgagg ataaagctat tcaaaaagac aaatctggga tgatgcagtg
     2881 tgtcattgct gttgctgaca aagtgtttga tgctttcctg aacatgatgg cagaaaaagc
     2941 taagaccaag gagaatgaag aggagctgga gcggcatgca cagtttctgc tggtgaactt
     3001 caaccacatt cataagagga taaggagagt agctgacaaa tacctatctg gtctggtgga
     3061 taagtttcca cacttgctct ggagcgggac tgtgctgaag accatgttgg acatcctgca
     3121 gaccctgtca ctatcactaa gtgctgatat tcacaaggac caaccttact atgacattcc
     3181 tgatgccccc tatcggatca cggttcctga cacatacgaa gcccgggaga gcatcgtgaa
     3241 agactttgct gcgcgctgtg gaatgatcct acaggaagcc atgaagtggg cacccacggt
     3301 cactaagtcc cacctacagg aatatctgaa caagcatcag aactgggtgt caggactgtc
     3361 tcagcacaca ggattggcca tggccactga gagcatcctt cattttgccg gctacaacaa
     3421 gcagaacaca actcttgggg caactcagct aactgagcgt ccggcctgtg tgaagaaaga
     3481 ctactccaac ttcatggcat ccttgaattt gcgcaaccgc tatgccggcg aggtacatgg
     3541 aatgattcgg ttctcaggtg ccacaggcca gatgtctgac ctgaacaaaa tgatggtcca
     3601 agatctgatc acagctttgg accacagtca tcctcagcac tatacacaag ccatgttcaa
     3661 attgacggca atgctcatta gcagcaaaga ttgtgatcca cagctccttc atcatctgtg
     3721 ttggggtccc ctccgaatgt tcaatgagca tggcatggag actgcactag cctgctggga
     3781 gtggctgctg gctggcaaga atggagtgga agtgccgttt atgcgggaga tggcaggagc
     3841 ctggcacatg acagtagagc agaagttcgg cctcttttct gtggaaacaa aggaagcaga
     3901 ccccctggca gcatcagaag caagtcagcc aagaccttgt ccccccgaag tgacacctca
     3961 ctacatctgg atcgatttcc tggtccagcg gtttgagatt gccaagtact gtagctctga
     4021 ccaggtggag atcttctcaa gcctgctgca gcgctccatg tccctcaata ttggtggagc
     4081 cagggggagc atgaacaggc atgtggcagc cattggacca cgtttcaaac tgctgacctt
     4141 ggggctgtcc ctcctgcatg ctgatgtggt tccaaatgca accattcgaa acgtgcttcg
     4201 agagaagata tactcaacag cctttgacta tttcagttgt ccaccaaagt ttcctactca
     4261 aggagaaaag aggcttcgtg aagatataag cataatgatt aagttttgga ctgccatgtt
     4321 ctcagataag aagtacctga ctgccagcca gctcgttccc ccagataatc aggacacccg
     4381 aagcaacttg gatataactg tgggctctcg acaacaggct acccagggct ggatcaacac
     4441 ttaccccctc tccagtggca tgtcaaccat ctccaaaaag tcaggtatgt ctaagaaaac
     4501 caaccggggc tcccagctgc acaagtacta catgaaacgc aggacactgc tgctgtcact
     4561 attggccact gagatcgaac gcctcatcac ttggtacaac ccactgtcag ccccagagct
     4621 agagctggac caggcaggag agaacagcgt tgccaactgg aggtccaagt acatcagctt
     4681 gagtgagaag cagtggaagg acaacgtgaa ccttgcctgg agcatctctc catacctcgc
     4741 tgtgcagctg ccagccaggt tcaagaatac agaagccatt ggaaatgaag tgacccgtct
     4801 cgttcgcttg gatcctggag ctgttagtga tgtccctgaa gccatcaagt tccttgtcac
     4861 ctggcacacc atcgatgctg atgctccaga gctcagccat gttttatgct gggcacccac
     4921 ggacccacct acaggcctct cctacttctc cagcatgtac ccaccacacc ctctcactgc
     4981 acagtatggg gtaaaagtcc tgcgatcctt ccctccggat gccattctgt tctacatccc
     5041 ccagattgtg caggccctca gatatgacaa gatgggctat gtgcgtgagt acattctctg
     5101 ggctgcagcc aaatcccagc ttctggcaca ccagtttatt tggaacatga aaactaacat
     5161 ctaccttgat gaagaagggc accagaaaga ccctgacatc ggtgacctcc tggagcagtt
     5221 agtagaagag atcacagggt ctctatcagg cccagccaaa gatttctatc aacgggaatt
     5281 tgatttcttt aacaagatca caaacgtgtc agccatcatc aagccctacc ctaaaggcga
     5341 tgaaaggaag aaggcttgtc tgtcagctct gtctgaagtg aaggtgcagc ctggctgcta
     5401 cctgcccagc aaccctgaag caatcgtgct agacattgac tacaagtcag ggacaccaat
     5461 gcagagtgcc gcaaaagcgc cgtatctggc caagtttaag gtgaagagat gcggagtgag
     5521 tgaacttgaa aaagaaggtc tgcagtgccg ctcagacgca gaggatgaat gcttcagcca
     5581 agaggctgat ggcaagaaga tctgctggca agcagccatc ttcaaagtgg gggatgactg
     5641 ccgacaggac atgttagccc tgcagatcat cgacctcttc aagaacatct tccagctggt
     5701 tggcctggac ctcttcgttt ttccttatcg ggtagtggct actgcccctg ggtgcggagt
     5761 gattgagtgc atcccagact gtacctcccg ggaccagctg ggccgccaga ctgactttgg
     5821 catgtatgac tactttacac gccagtatgg ggatgagtct accttggcct tccagcaggc
     5881 acgctacaac tttatcagaa gcatggccgc ctatagcctc ctgctgttcc tgctgcagat
     5941 caaggacagg cacaatggca acatcatgct ggacaagaag gggcacatca tccacatcga
     6001 ctttggcttc atgtttgaaa gctcaccggg tggcaacctt ggctgggaac cagacatcaa
     6061 gctgacagac gagatggtga tgattatggg gggcaaaatg gaagccacac ccttcaagtg
     6121 gttcatggag atgtgtgtcc gaggttatct ggctgtgcgc ccctatatgg atgcagtcgt
     6181 ctccctggtt acactcatgt tggacacagg cctgccctgt tttcgtggcc agacaatcaa
     6241 acttttgaaa cacaggttca gcccgaacat gacagagcgg gaggctgcga attttatcat
     6301 gaaggtcatc cagaactgtt tcctcagcaa caggagccgg acctacgaca tgatccagta
     6361 ctaccagaat gatatcccct actgagaagc ctagcctagc acctgaagcc ttgagtgtca
     6421 cagagcaact cgtcctgcca ccgtaggcct ggctgtgggg gcaacgggca catggaccgt
     6481 tgatgcacac gtgtgagtct ctttcacact ggacacattc cgtttgttca cccgtcatac
     6541 tacattggta catgaagtat tttcttgtat aaacatgaaa aaaataaaaa tcccaaaatg
     6601 tg
//
