LOCUS       NM_025951               3143 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus phosphatidylinositol 4-kinase type 2 beta (Pi4k2b),
            transcript variant 1, mRNA.
ACCESSION   NM_025951
VERSION     NM_025951.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Wuttke,A., Sagetorp,J. and Tengholm,A.
  TITLE     Distinct plasma-membrane PtdIns(4)P and PtdIns(4,5)P2 dynamics in
            secretagogue-stimulated beta-cells
  JOURNAL   J Cell Sci 123 (Pt 9), 1492-1502 (2010)
   PUBMED   20375060
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Tamplin,O.J., Kinzel,D., Cox,B.J., Bell,C.E., Rossant,J. and
            Lickert,H.
  TITLE     Microarray analysis of Foxa2 mutant mouse embryos reveals novel
            gene expression and inductive roles for the gastrula organizer and
            its derivatives
  JOURNAL   BMC Genomics 9, 511 (2008)
   PUBMED   18973680
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3143)
  AUTHORS   Jones,D.R., Bultsma,Y., Keune,W.J., Halstead,J.R., Elouarrat,D.,
            Mohammed,S., Heck,A.J., D'Santos,C.S. and Divecha,N.
  TITLE     Nuclear PtdIns5P as a transducer of stress signaling: an in vivo
            role for PIP4Kbeta
  JOURNAL   Mol Cell 23 (5), 685-695 (2006)
   PUBMED   16949365
  REMARK    GeneRIF: UV irradiation increases nuclear PtdIns5P levels via
            inhibition of the activity of the beta isoform of PtdIns5P 4-kinase
            (PIP4Kbeta), an enzyme that can phosphorylate and remove PtdIns5P.
REFERENCE   4  (bases 1 to 3143)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   5  (bases 1 to 3143)
  AUTHORS   Mora,S., Durham,P.L., Smith,J.R., Russo,A.F., Jeromin,A. and
            Pessin,J.E.
  TITLE     NCS-1 inhibits insulin-stimulated GLUT4 translocation in 3T3L1
            adipocytes through a phosphatidylinositol 4-kinase-dependent
            pathway
  JOURNAL   J Biol Chem 277 (30), 27494-27500 (2002)
   PUBMED   12011096
  REMARK    GeneRIF: PI 4-kinase functions to negatively regulate GLUT4
            translocation through its interaction with NCS-1.
REFERENCE   6  (bases 1 to 3143)
  AUTHORS   Balla,A., Tuymetova,G., Barshishat,M., Geiszt,M. and Balla,T.
  TITLE     Characterization of type II phosphatidylinositol 4-kinase isoforms
            reveals association of the enzymes with endosomal vesicular
            compartments
  JOURNAL   J Biol Chem 277 (22), 20041-20050 (2002)
   PUBMED   11923287
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY140598.1, AK035523.1 and AK011751.1.
            
            On Nov 2, 2013 this sequence version replaced NM_025951.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK035523.1, AK170730.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                BY140598.1         1-17
            18-2318             AK035523.1         3-2303
            2319-3136           AK011751.1         2282-3099
            3137-3143           AK035523.1         3121-3127
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 28.63 cM"
     gene            1..3143
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /note="phosphatidylinositol 4-kinase type 2 beta"
                     /db_xref="GeneID:67073"
                     /db_xref="MGI:MGI:1914323"
     exon            1..395
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..1555
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /EC_number="2.7.1.67"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phosphatidylinositol 4-kinase type II-beta"
                     /codon_start=1
                     /product="phosphatidylinositol 4-kinase type 2-beta
                     isoform 1"
                     /protein_id="NP_080227.2"
                     /db_xref="CCDS:CCDS19287.1"
                     /db_xref="GeneID:67073"
                     /db_xref="MGI:MGI:1914323"
                     /translation="MAEACEPTRPSEDEDEEREPLLPRVAWAQPRRVAPGSAVRMQAD
                     EGADVLREPATDEPPAVSGEGSISASLSTELDRTRTTSSETNTFLEDPEFADIVLKAE
                     QAIEIGVFPERISQGSSGSYFVKDSKRNIIGVFKPKSEEPYGQLNPKWTKYVHKVCCP
                     CCFGRGCLLPNQGYLSEAGAYLVDVKLNLGIVPKTKVVWLVSETFNYSAIDRAKSRGK
                     KYALEKVPKVGRKFHRIGLPPKVGSFQLFVKDYKEAEYWLRRFEAEPLPENIRKQFQS
                     QFEKLVILDYIIRNTDRGNDNWLVKYDEMKYAKKIESEESNWIDNKQLLIKIAAIDNG
                     LAFPFKHPDEWRAYPFHWAWLPQAKVPFSEETRNLILPYISDMNFVQDLCEDLYELFK
                     TDKGFDRAAFENQMSVMRGQILNLTQALRDGKSPMQLAQMPCVIVECSKSGSQGRVVH
                     LGSSFTQTVHCRKPFFSSW"
     exon            396..532
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            533..733
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            734..865
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            866..1019
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1087
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1187
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1321
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1381
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..3143
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3119..3124
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3139
                     /gene="Pi4k2b"
                     /gene_synonym="2610042N09Rik; 4933409G22Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtggccggcg gctgggcgtg gccggcgcgg gctggtggac tggtgaggtg gcgtcggtgg
       61 gagcagtccc ccgcggtttg gcgtgatgct ggggtcccag tccggagccc ggcggctctg
      121 agcgccggag ccgccccggg acatcatggc ggaggcctgc gagcccaccc gcccctcgga
      181 ggacgaggac gaggagcggg agccgctgct gcctcgcgtt gcctgggccc agccgcggag
      241 ggtcgcgccc gggagcgccg tgagaatgca ggcggacgag ggcgcggatg tcctccgcga
      301 gcccgctacc gacgagccgc cggcggtgtc cggggaaggg tcgatctccg cgagcttgtc
      361 caccgagctg gatcggaccc gcactacgag ctcagaaaca aatacattct tggaagatcc
      421 agaatttgct gatattgtat tgaaagcaga gcaagcaata gaaattggag ttttcccaga
      481 aagaatttct caagggtcaa gtggaagtta ctttgtaaag gattctaaaa ggaatattat
      541 tggcgtgttt aaacccaaat cagaagagcc ttatggccag ctgaacccca agtggaccaa
      601 gtacgtccat aaggtttgct gtccctgctg ctttggccga ggctgcctgc ttcctaacca
      661 ggggtacctt tctgaagctg gtgcctacct ggtggatgtc aaacttaatc tgggtattgt
      721 gcctaaaaca aaggtggttt ggcttgtcag tgagacattt aactacagtg caattgaccg
      781 tgcaaaatca agaggcaaaa agtatgcctt agagaaagtg ccaaaagtag gcagaaagtt
      841 ccatcggata ggacttcctc ctaaggttgg ttcctttcag ctgtttgtga aagattacaa
      901 ggaggctgag tattggctta ggaggtttga agcggagcct ctgcccgaga acattagaaa
      961 acagtttcag tcacagtttg aaaaattagt gattctggat tacatcatca ggaacacaga
     1021 caggggaaat gataactggc tagtcaaata tgatgagatg aagtatgcaa agaaaattga
     1081 aagtgaggaa tcaaactgga ttgataataa acaattgctt attaaaatag ctgcaattga
     1141 taatgggcta gcatttccct ttaagcatcc tgatgaatgg cgagcatatc catttcactg
     1201 ggcttggctt cctcaagcaa aggttccttt ttctgaagag actcgaaact tgattctacc
     1261 ctatatttct gacatgaact ttgtacaaga cttgtgtgaa gatctttatg aactttttaa
     1321 gactgacaaa ggatttgaca gagctgcttt tgaaaatcag atgtctgtga tgagaggcca
     1381 gatcttaaac ctcactcagg cactgagaga cgggaagagt cctatgcagc tggcacagat
     1441 gccatgtgtg atcgtggagt gcagcaaaag tggcagccag ggccgtgtgg tacacctggg
     1501 cagctccttc acccagacgg tccactgcag gaaaccgttc ttctcctcct ggtagcagat
     1561 gtaaggggaa caactgtgtg gtgttactgt gctgataaaa tattacagtg atgtaaatct
     1621 gctgctgaga gcaccagtcg ccagaacctc aaataattca agtctttaaa tatatagaaa
     1681 ataatactat attttgaatg taatcaaaag tttacgattt tttgtccaaa tattctattt
     1741 cagggaagaa gtgctattat ctatattgta tttttataga aacattgtat tttatgttgt
     1801 tattacttta aaaagtaact tcatggttta cacatgctag catgactgta gttctagaga
     1861 attccagcta gaattcctgt attgactttg aatacaggaa cattttgtat actttttaaa
     1921 cacgagaaga atcttcttgc attcattcca aaagacttga ggatggccaa gccagaaact
     1981 gctcttgtag actcccaaca gaggacccag tgggtagagc ggacagtgat agtaaagcaa
     2041 ctataccttc agtttaaaag ctgctgtttt aaaactggaa tcaagtgccc aaaggtggtc
     2101 attaccggga acctgaaagt taagttctgc ctaagtgaca gatgactata gtaagttttg
     2161 aattttgata tatttgttga tgaattattc tgttttagtg aagggggaaa aaatctaaag
     2221 acatctcccc tcctttttga aaacaagaaa taactgaaaa ttgaatggaa taatatgaaa
     2281 ttaaggtaga tgtccagtat tttaaaatta caatattgcc caaaaaaaat tcttggtttt
     2341 tttttttttc tcatatggga tcaaataatt taaaattagt acttcaaaac actaatctta
     2401 atcagttctg tactctagtc agtatggaac atcacttcaa agcaagccat tccctgaagc
     2461 ttatactgaa gtcaggaaca cggtgctcag acacgcctgg ggcgactgga agctgatctc
     2521 accattcact ttaggttgct gtcttagggg ttttttaatg agctattttt gtatgagaag
     2581 gtagttacat ccaggtggtt aaattaaggt gtggtagtca ccgtcttggt gcaggtaagg
     2641 agtgagatac cactgccctg aggagtcagt tggaaactcg tcctcctacc tcttctgacc
     2701 cacctcccat tagctggggt ttgagataat ggcaacctga ctggagtatc aaactatacc
     2761 accagctgtt ggtaaatact agtcattgat ttgtgccttt cttctgcagt agagcgcata
     2821 cttgcctctg ctgtggtacg gctttcacaa aggattttct tttcattgtt ttgaaaattg
     2881 ctatattaat ttttataaca gagaacctag taaattgcta ctgtttatag tctaaaattg
     2941 aaatagttaa agactatttt aaacatctga ccatgttaga atagttgggg tatttaaaaa
     3001 ttacaagtaa aagtacttac gctgtacttt gtaagcattc atgaacttaa ttcttattta
     3061 aatagtgagg agttttttaa tgagcatatt tatattgtca aatatgataa acaaaaggaa
     3121 taaaaatcat tgtcttgaag ctc
//
