LOCUS       NM_145501               3771 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a),
            mRNA.
ACCESSION   NM_145501 XM_915540
VERSION     NM_145501.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Khundadze,M., Ribaudo,F., Hussain,A., Stahlberg,H.,
            Brocke-Ahmadinejad,N., Franzka,P., Varga,R.E., Zarkovic,M.,
            Pungsrinont,T., Kokal,M., Ganley,I.G., Beetz,C., Sylvester,M. and
            Hubner,C.A.
  TITLE     Mouse models for hereditary spastic paraplegia uncover a role of
            PI4K2A in autophagic lysosome reformation
  JOURNAL   Autophagy 17 (11), 3690-3706 (2021)
   PUBMED   33618608
  REMARK    GeneRIF: Mouse models for hereditary spastic paraplegia uncover a
            role of PI4K2A in autophagic lysosome reformation.
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Lopes da Silva,M., O'Connor,M.N., Kriston-Vizi,J., White,I.J.,
            Al-Shawi,R., Simons,J.P., Mossinger,J., Haucke,V. and Cutler,D.F.
  TITLE     Type II PI4-kinases control Weibel-Palade body biogenesis and von
            Willebrand factor structure in human endothelial cells
  JOURNAL   J Cell Sci 129 (10), 2096-2105 (2016)
   PUBMED   27068535
REFERENCE   3  (bases 1 to 3771)
  AUTHORS   Robinson,J.W., Leshchyns'ka,I., Farghaian,H., Hughes,W.E.,
            Sytnyk,V., Neely,G.G. and Cole,A.R.
  TITLE     PI4KIIalpha phosphorylation by GSK3 directs vesicular trafficking
            to lysosomes
  JOURNAL   Biochem J 464 (1), 145-156 (2014)
   PUBMED   25083612
REFERENCE   4  (bases 1 to 3771)
  AUTHORS   Mossinger,J., Wieffer,M., Krause,E., Freund,C., Gerth,F., Krauss,M.
            and Haucke,V.
  TITLE     Phosphatidylinositol 4-kinase IIalpha function at endosomes is
            regulated by the ubiquitin ligase Itch
  JOURNAL   EMBO Rep 13 (12), 1087-1094 (2012)
   PUBMED   23146885
  REMARK    GeneRIF: Itch directly associates with and ubiquitinates
            PI4KIIalpha, and both proteins colocalize on endosomes containing
            Wnt-activated frizzled 4 (Fz4) receptor
REFERENCE   5  (bases 1 to 3771)
  AUTHORS   Srivastava,U., Paigen,B.J. and Korstanje,R.
  TITLE     Differences in health status affect susceptibility and mapping of
            genetic loci for atherosclerosis (fatty streak) in inbred mice
  JOURNAL   Arterioscler Thromb Vasc Biol 32 (10), 2380-2386 (2012)
   PUBMED   22837474
REFERENCE   6  (bases 1 to 3771)
  AUTHORS   Xu,Z., Huang,G. and Kandror,K.V.
  TITLE     Phosphatidylinositol 4-kinase type IIalpha is targeted specifically
            to cellugyrin-positive glucose transporter 4 vesicles
  JOURNAL   Mol Endocrinol 20 (11), 2890-2897 (2006)
   PUBMED   16772528
  REMARK    GeneRIF: The unique N terminus of PI4K type IIalpha is required for
            the targeting of the enzyme to cellugyrin-positive vesicles.
REFERENCE   7  (bases 1 to 3771)
  AUTHORS   Ghazalpour,A., Doss,S., Zhang,B., Wang,S., Plaisier,C.,
            Castellanos,R., Brozell,A., Schadt,E.E., Drake,T.A., Lusis,A.J. and
            Horvath,S.
  TITLE     Integrating genetic and network analysis to characterize genes
            related to mouse weight
  JOURNAL   PLoS Genet 2 (8), e130 (2006)
   PUBMED   16934000
REFERENCE   8  (bases 1 to 3771)
  AUTHORS   Salazar,G., Craige,B., Wainer,B.H., Guo,J., De Camilli,P. and
            Faundez,V.
  TITLE     Phosphatidylinositol-4-kinase type II alpha is a component of
            adaptor protein-3-derived vesicles
  JOURNAL   Mol Biol Cell 16 (8), 3692-3704 (2005)
   PUBMED   15944223
  REMARK    GeneRIF: PI4KIIalpha is present on adaptor protein (AP) -3
            organelles where it regulates AP-3 function
REFERENCE   9  (bases 1 to 3771)
  AUTHORS   Brown,A.C., Olver,W.I., Donnelly,C.J., May,M.E., Naggert,J.K.,
            Shaffer,D.J. and Roopenian,D.C.
  TITLE     Searching QTL by gene expression: analysis of diabesity
  JOURNAL   BMC Genet 6, 12 (2005)
   PUBMED   15760467
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 3771)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY732177.1, AK030725.1 and AC133503.4.
            
            On Feb 1, 2006 this sequence version replaced NM_145501.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030725.1, BC110363.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-280               BY732177.1         2-281
            281-926             AK030725.1         246-891
            927-927             AC133503.4         42879-42879         c
            928-3640            AK030725.1         893-3605
            3641-3771           AC133503.4         26082-26212         c
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 35.74 cM"
     gene            1..3771
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /note="phosphatidylinositol 4-kinase type 2 alpha"
                     /db_xref="GeneID:84095"
                     /db_xref="MGI:MGI:1934031"
     exon            1..502
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..1507
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /EC_number="2.7.1.67"
                     /note="phosphatidylinositol 4-kinase type II-alpha"
                     /codon_start=1
                     /product="phosphatidylinositol 4-kinase type 2-alpha"
                     /protein_id="NP_663476.1"
                     /db_xref="CCDS:CCDS29821.1"
                     /db_xref="GeneID:84095"
                     /db_xref="MGI:MGI:1934031"
                     /translation="MDETSPLVSPERAQPPEYTFPSGSGAHFPQVPGGAVRVAAAAGS
                     GPSPPCSPGHDRERQPLLDRARGAAAQGQTHTVAVQAQALAAQAAVAAHAVQTHRERN
                     DFPEDPEFEVVVRQAEVAIECSIYPERIYQGSSGSYFVKDSQGRIVAVFKPKNEEPYG
                     HLNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLAS
                     ETFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRR
                     FEAEPLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDCPMDNSSCRDTDWVM
                     VREPVIKVAAIDNGLAFPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPN
                     FIKDLEEDLYELFKRDPGFDRGQFHKQIAVMRGQILNLTQALKDNKSPLHLVQMPPVI
                     VETARSHQRSASESYTQSFQSRKPFFSWW"
     exon            503..703
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /inference="alignment:Splign:2.1.0"
     exon            704..835
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /inference="alignment:Splign:2.1.0"
     exon            836..989
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1051
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1151
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1285
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1345
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..3771
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3568..3573
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /note="hexamer: AACAAA"
     polyA_site      3577
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /note="major polyA site"
     regulatory      3622..3627
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /note="hexamer: GATAAA"
     polyA_site      3645
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
     regulatory      3734..3739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
                     /note="hexamer: GATAAA"
     polyA_site      3763
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4k2"
ORIGIN      
        1 ggattggtca cggccgcgag ctcgctggtg tggagcgcgc cgggtccccg agccggcggc
       61 ctgagggatg gacgagacga gcccgctagt gtcccccgag cgggcccaac ccccggagta
      121 caccttcccg tcgggctccg gagctcactt tccgcaagta ccggggggcg cggtccgcgt
      181 ggcggcggcg gccggctccg gcccgtcacc gccgtgctcg cccggccacg accgggagcg
      241 gcagcccctg ctggaccggg cccggggcgc ggcggcgcag ggccagaccc acacggtggc
      301 ggtgcaggcc caggccctgg ccgcccaagc ggccgtggcg gcgcacgccg ttcagaccca
      361 ccgcgagcgg aacgacttcc cggaggaccc cgagttcgag gtggtggtgc ggcaggccga
      421 ggttgccatc gagtgcagca tctatcccga gcgcatctac cagggctcca gtggaagcta
      481 cttcgtcaag gactctcagg ggagaatcgt tgctgtcttc aaacccaaga atgaagagcc
      541 atacgggcac cttaacccta agtggaccaa gtggctgcag aagctgtgct gcccctgctg
      601 ctttggccga gactgccttg ttctcaacca gggctatctc tcagaggcag gggctagcct
      661 ggtggaccaa aaactggaac tcaacattgt accacgtaca aaggtagtat acctggccag
      721 tgaaaccttc aactacagtg ccattgatcg agtaaagtcc aggggcaagc ggcttgcact
      781 agagaaagtg ccaaaagttg ggcagcggtt taaccgaatc ggcctgccac caaaggtcgg
      841 gtcattccag ctcttcgttg aaggctacaa agatgcagac tattggctgc ggcgttttga
      901 agcagaacct ctccctgaga acacgaaccg acagctgcta ttgcagtttg agcggttggt
      961 ggtcctggac tacatcatcc gcaacactga ccgaggcaat gacaactggt tgatcaaata
     1021 tgactgtccg atggataatt ctagctgtcg ggacacagat tgggtgatgg tgagggagcc
     1081 tgttatcaag gtggctgcca tagacaacgg gctagctttc ccactgaagc atcctgactc
     1141 ctggagggca tatccttttt actgggcctg gctgcctcag gcgaaagtcc cgttctctca
     1201 ggagatcaaa gatttgattc ttccaaagat ttcagaccct aacttcatca aggacttgga
     1261 ggaggaccta tatgaactct tcaagagaga tcctggcttc gacaggggcc agttccataa
     1321 gcagattgct gtcatgagag gccagatcct aaatttgacc caggccctga aagacaataa
     1381 gagccccctg cacctcgtcc agatgccacc tgtgattgtc gagacggccc gctctcacca
     1441 gcggtctgca agcgaatcct acacacagag ctttcagagt cggaagccct tcttttcatg
     1501 gtggtagccc cagaggcagg cagaagaaat cctgcaggtg acaggctggg aggaagcctg
     1561 gagtcccagg ttcgcagaag ccagagaagc tggcgggcgc acagcagagc ctttcagagc
     1621 ccttcctctt tgcttgtcac ctccctcagg gctttcctac ccacagggag aagcacaatg
     1681 gggggacaag agtgttcctg gaccttcaga gtgttgggga ggctcctgtc caggcaagga
     1741 gctcaggcgg ctgaggagga gccactccct cctagcattt ggtggctggt tgggaaagtc
     1801 ccatctctcc ctgacaaccc tctccatcca ggttacctaa tatattctgc atcagaaacc
     1861 aacaaaacaa aaacaaaaga aacaaattta aatgcttgta ggaaaaccta cattagatcc
     1921 tctgacatca gaagccctta atctagaacc taaatttgct cagacacagg cgctctgata
     1981 cctagcactt gtttactcca tgaactgagc ggtcagaggt ccatcgctgg cccacggaca
     2041 gaagccattc ctttctggtt ttgccaaatt gagcctcctc ttgccacact ggtactgact
     2101 tcgtgtggca gtgagcaggt tcatgctctg ctcctctaag gctctgcaca caggcttttt
     2161 ctctgccctg ctgtaacttg cccggcactc atctgaggtg ggaggggcta gccttggcag
     2221 tgtgctctgc caggagtggt tgtgtgacgc ggaggtgatt gacttggcct ggttgcatgc
     2281 tgggtgctag gagggttctg tgggagcctg gcacagcctc actcctggtc cttagttgcg
     2341 gccttcccta cctttctgtt tggtagtcta gtttccccca ttttaaaatg agaagttcag
     2401 tttgggttct ggagtggctc tcagtaggtg ttgtgggtca gtaggttcat gggcagccag
     2461 gacagcatct ctgttcttca tgtgttaact gctgctgcct ggagagaaca ggaccgtgtt
     2521 ctgcccccta accggcgtgc cccttggctc ctgccttccg tcttgggatg gtttcttctc
     2581 agcttcttga agagaggcct tcggacagtt ccccagtgcc tgcccagctc agcgtgcatc
     2641 ttcctgcttc catgtcttct cccttgccag gtgtttgaac cctttctaca ccaaagcaga
     2701 gaactgactt caggagggag tgaaaagggg gccgagaggc cgtaggcagc ggcagtggtc
     2761 acaccctgtg ctgtggaccc ttgagtgttc tcacactcta cagttggtca gtcagccttg
     2821 gaaagaaatg tcctgaaggc gtgaaacaac caaagacctg gagtgaggag cacaactgtg
     2881 cctcagctgc ttgccgggct cccaccgcct agaggcaggg ctgaagacag ccttctgtca
     2941 ggtgtggcag gctgctccct gcccattcca acccaggagc cagcttgcta tgggctttca
     3001 ctcagcaaca tgaatctcca agcactggat atgcatgccc aatggacagt cgctgttgtt
     3061 tgccccatct tgacaatccc atccagccca cgtggactct ggcctcagaa ccttgtttga
     3121 gagaatctct ctctctcctc gccacaggag ccagcagggg tccaggtgtg tgccggtctt
     3181 cacagatgtt tccacaccag ccgggtggcg agacttcccc ctggagtgct ggagagaact
     3241 tagtcctgcc ccagaccagg tttgccatca ggaggaaatt gggtccaatg gaagaggggg
     3301 ccggtgccag gcccagtggg caagggtgca ggcagcctta cctgcctccc tccctcgaag
     3361 cttgtactca agttgccttg aatgtggact tgggagtgcc aggagcccag agtgtcaggt
     3421 acttcagaac agccgcccct ctgccagcct taggcctgtc ccctgtgtct aggagctgaa
     3481 gctggctggt gagcgctgct gccctgttct tctaatgcac tgtagaaatt gtatgtattg
     3541 tatttaaatc aatgcaaatg tatgaataac aaatccagtt ctgacctctc tgtccagttt
     3601 cctttggggg aagaaagaca agataaatgc agttttaagg acaaagacac atttctgtgg
     3661 agttatgggg acttgactct tgaaaaccaa gtgttttctt aagacatttc aaaaactgac
     3721 atgagtctgc attgataaaa cacatacttg cttttggtat gaatatttac t
//
