LOCUS       NM_023734               2245 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus peptidase inhibitor 16 (Pi16), mRNA.
ACCESSION   NM_023734 XM_915667
VERSION     NM_023734.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Coren,L., Zaffryar-Eilot,S., Odeh,A., Kaganovsky,A. and Hasson,P.
  TITLE     Fibroblast diversification is an embryonic process dependent on
            muscle contraction
  JOURNAL   Cell Rep 43 (12), 115034 (2024)
   PUBMED   39636726
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Hashimoto,K., Ohira,M., Kodama,A., Kimoto,M., Inoue,M., Tone,S.,
            Usui,Y., Hanashima,A., Goto,T., Ogura,Y., Ujihara,Y. and Mohri,S.
  TITLE     Loss of connectin novex-3 leads to heart dysfunction associated
            with impaired cardiomyocyte proliferation and abnormal nuclear
            mechanics
  JOURNAL   Sci Rep 14 (1), 13727 (2024)
   PUBMED   38877142
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2245)
  AUTHORS   Sun,Y., Lin,S., Wang,H., Wang,L., Qiu,Y., Zhang,F., Hao,N., Wang,F.
            and Tan,W.
  TITLE     Regulatory role of PI16 in autoimmune arthritis and intestinal
            inflammation: implications for Treg cell differentiation and
            function
  JOURNAL   J Transl Med 22 (1), 327 (2024)
   PUBMED   38566233
  REMARK    GeneRIF: Regulatory role of PI16 in autoimmune arthritis and
            intestinal inflammation: implications for Treg cell differentiation
            and function.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2245)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 2245)
  AUTHORS   Wang,F., Gu,X., Lin,S., Wu,Q., Sun,Y., Zhang,Q., Luo,A., Feng,X.,
            Wang,L., Xu,L., Sun,W. and Tan,W.
  TITLE     Peptidase inhibitor 16 promotes inflammatory arthritis by
            suppressing Foxp3 expression via regulating K48-linked ubiquitin
            degradation Bmi-1 in regulatory T cells
  JOURNAL   Clin Immunol 259, 109883 (2024)
   PUBMED   38147957
  REMARK    GeneRIF: Peptidase inhibitor 16 promotes inflammatory arthritis by
            suppressing Foxp3 expression via regulating K48-linked ubiquitin
            degradation Bmi-1 in regulatory T cells.
REFERENCE   6  (bases 1 to 2245)
  AUTHORS   Angueira,A.R., Sakers,A.P., Holman,C.D., Cheng,L., Arbocco,M.N.,
            Shamsi,F., Lynes,M.D., Shrestha,R., Okada,C., Batmanov,K.,
            Susztak,K., Tseng,Y.H., Liaw,L. and Seale,P.
  TITLE     Defining the lineage of thermogenic perivascular adipose tissue
  JOURNAL   Nat Metab 3 (4), 469-484 (2021)
   PUBMED   33846639
REFERENCE   7  (bases 1 to 2245)
  AUTHORS   Deng,M., Yang,S., Ji,Y., Lu,Y., Qiu,M., Sheng,Y., Sun,W. and
            Kong,X.
  TITLE     Overexpression of peptidase inhibitor 16 attenuates angiotensin
            II-induced cardiac fibrosis via regulating HDAC1 of cardiac
            fibroblasts
  JOURNAL   J Cell Mol Med 24 (9), 5249-5259 (2020)
   PUBMED   32227584
  REMARK    GeneRIF: Overexpression of peptidase inhibitor 16 attenuates
            angiotensin II-induced cardiac fibrosis via regulating HDAC1 of
            cardiac fibroblasts.
REFERENCE   8  (bases 1 to 2245)
  AUTHORS   Singhmar,P., Trinh,R.T.P., Ma,J., Huo,X., Peng,B., Heijnen,C.J. and
            Kavelaars,A.
  TITLE     The fibroblast-derived protein PI16 controls neuropathic pain
  JOURNAL   Proc Natl Acad Sci U S A 117 (10), 5463-5471 (2020)
   PUBMED   32079726
  REMARK    GeneRIF: these findings support a model in which PI16 promotes
            neuropathic pain by mediating a cross-talk between fibroblasts and
            the endothelial barrier leading to barrier opening, cellular
            influx, and increased pain
REFERENCE   9  (bases 1 to 2245)
  AUTHORS   Regn,M., Laggerbauer,B., Jentzsch,C., Ramanujam,D., Ahles,A.,
            Sichler,S., Calzada-Wack,J., Koenen,R.R., Braun,A., Nieswandt,B.
            and Engelhardt,S.
  TITLE     Peptidase inhibitor 16 is a membrane-tethered regulator of chemerin
            processing in the myocardium
  JOURNAL   J Mol Cell Cardiol 99, 57-64 (2016)
   PUBMED   27539859
  REMARK    GeneRIF: our data indicate that PI16 suppresses chemerin activation
            in the myocardium and suggest that this circuit may be part of the
            cardiac stress response.
REFERENCE   10 (bases 1 to 2245)
  AUTHORS   Frost,R.J. and Engelhardt,S.
  TITLE     A secretion trap screen in yeast identifies protease inhibitor 16
            as a novel antihypertrophic protein secreted from the heart
  JOURNAL   Circulation 116 (16), 1768-1775 (2007)
   PUBMED   17909105
  REMARK    GeneRIF: PI16, a novel protein secreted from the heart, is strongly
            upregulated early in heart failure and inhibits growth of
            cardiomyocytes both in vitro and in vivo
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK157854.1, AI587910.1 and AB046537.1.
            
            On Oct 13, 2006 this sequence version replaced NM_023734.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB046537.1, BC039124.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-721               AK157854.1         7-727
            722-1106            AI587910.1         1-385
            1107-2245           AB046537.1         756-1894
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 15.22 cM"
     gene            1..2245
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /note="peptidase inhibitor 16"
                     /db_xref="GeneID:74116"
                     /db_xref="MGI:MGI:1921366"
     exon            1..510
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /inference="alignment:Splign:2.1.0"
     CDS             334..1830
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /note="protease inhibitor 16; cysteine-rich protease
                     inhibitor"
                     /codon_start=1
                     /product="peptidase inhibitor 16 precursor"
                     /protein_id="NP_076223.3"
                     /db_xref="CCDS:CCDS28595.2"
                     /db_xref="GeneID:74116"
                     /db_xref="MGI:MGI:1921366"
                     /translation="MHGSCSPWVMLPPPLLLLLLLIATGPTTALTEDEKQTMVDLHNQ
                     YRAQVSPPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRRGENLFAITDEGMDVP
                     LAVGNWHEEHEYYNFSTATCDPNQMCGHYTQVVWSKTERIGCGSHFCETLQGVEEANI
                     HLLVCNYEPPGNVKGRKPYQEGTPCSQCPLGYSCENSLCEPMRNPEKAQDSPPRVTEV
                     PSTRATEAPSSRETGTPSLATSETLHFSVTKVSDSLATESSPAVETKAPSSLATEGPS
                     SMATEAQAFVTEVPLVSARHMQPSVDEGPVNFLTSTHIPVPKSMDEEASKSSATSVSP
                     KKSLYPKMSLTESGESVPQIQEEAEPKDELSEPEAILPEAEAAPTEAEVELREPEAES
                     PKAESPEAEAESPLSSEALVPVLPAQERGGQKASLEHSGHPASPSLPTFPSASGNATG
                     GRTLALQSSWTGAENPEKADWDLKNSAHVWGPFLGLLLPSLLLLAGMV"
     exon            511..732
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /inference="alignment:Splign:2.1.0"
     exon            733..842
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /inference="alignment:Splign:2.1.0"
     exon            843..931
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1717
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1850
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..2245
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2224..2229
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /note="hexamer: AATAAA"
     polyA_site      2241
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /note="major polyA site"
ORIGIN      
        1 ctgaatgcca cagcacagcg ctgcagctgc ttctggaatt gtgtcccctg aggatctgtc
       61 cccctcagga gggatggtgg agctcatata gcctttggcc tgagcccttt actgagagaa
      121 aaggaggctg ttgccaagga ctccagggga gtggattctg ctctggtcca gacccaaggc
      181 acccagccct cctggagaca ttgtgtgagc cgggctgggc ctccagacac agcccctgct
      241 gccccacccc agccagggtg gtgcttgtgt gggaaggact ttaagtccag ctgccagacc
      301 cctgatcagg agaggcagag agggctggcc accatgcacg gttcctgcag cccgtgggtg
      361 atgttgccgc cgccactgct cctcctgctg ctgctgattg ccactggccc caccacagcc
      421 ctcacagagg acgagaagca aactatggtg gatcttcaca accagtaccg tgcccaggtg
      481 tccccgccag cctcagatat gctgcagatg aggtgggatg acgagctggc cgccttcgcc
      541 aaggcctacg ctcagaagtg cgtgtggggc cacaacaaag aacgcgggcg gcgcggagag
      601 aacttgtttg ccatcacgga cgagggcatg gacgtgccgc tggccgtggg gaactggcac
      661 gaggagcatg agtattacaa tttcagcacg gccacctgcg atccgaacca gatgtgcggc
      721 cactacactc aggtagtgtg gagcaagact gagagaattg gctgtggctc ccacttctgc
      781 gagacgctcc agggagtgga agaagctaac atccatttgc tggtgtgcaa ctatgagcct
      841 ccggggaacg tgaagggccg taagccctac caggagggga ctccttgctc ccagtgccct
      901 cttggctaca gctgtgagaa ctctctctgt gagcccatga gaaacccgga aaaggcgcag
      961 gattcgcctc caagggtgac cgaggtccct tccacccgcg caactgaagc cccaagctcc
     1021 agggaaaccg gtactccatc cctagcaacc tctgagactc tacatttctc ggtaacaaag
     1081 gtctcggact ccctggcaac cgagtcctca cctgcagtag aaacaaaggc accatcttcc
     1141 ttagcaaccg aaggcccctc ctccatggca acagaggctc aggcttttgt aactgaggtc
     1201 cccttggttt ctgcaaggca catgcagccc tcggtggatg aagggccagt taacttcctc
     1261 acatcaacac atatccctgt ccccaaatct atggacgaag aagccagcaa gtcgagcgca
     1321 acctccgtga gcccaaagaa atcgctgtac cccaagatgt ccctgacaga gtcaggagag
     1381 tccgtacccc aaatccagga ggaggctgaa cccaaggacg agttgtctga gcccgaggcc
     1441 atattgcccg aggcagaggc cgcaccgact gaggcagagg tcgagttgcg ggagcccgag
     1501 gctgagtctc ccaaggccga gtcgccagag gcagaggctg agtcgcctct ttccagtgag
     1561 gctttggtcc cagttcttcc agcccaggag cgtggtgggc agaaggcctc actggaacac
     1621 tctggccacc ctgcctcccc atccctgccc accttcccta gtgcttcggg taatgccaca
     1681 ggtgggcgca ccctggccct acaatcatcc tggacaggtg ctgagaaccc cgaaaaggcc
     1741 gactgggatt tgaagaattc tgctcacgtg tggggacctt tcctgggact gctgctgcct
     1801 tccctgctgc tgttggctgg catggtctga gggggacacc caaaggcctg ggagaagagg
     1861 tcagctgatc aactgctgtg tcctatccta tcgctcaaac aagataccac cgcctggcca
     1921 aagcccgtct ggaagggaaa ggctgtgtgg tccagcccat cagtaactgc acaccagggc
     1981 cctcccttgc cctttacagt ggagttctac agaggacatg attctgggga tacttgtctg
     2041 cttcccaggg gatggagggg tggggtgggg gaggggctgt ctgcctgcag gctctagggc
     2101 tccccatctc tgagtgccca tgtagcttgg acagcagacc ctgagggcag gtgaagaaca
     2161 agccccaacg aagtggggtt ctgtgggtgg ggagacaggg aatggaggca gccttgactc
     2221 ctgaataaag cctgtctgag agcct
//
