LOCUS       NP_076223                498 aa            linear   ROD 28-APR-2025
DEFINITION  peptidase inhibitor 16 precursor [Mus musculus].
ACCESSION   NP_076223 XP_920760
VERSION     NP_076223.3
DBSOURCE    REFSEQ: accession NM_023734.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 498)
  AUTHORS   Coren,L., Zaffryar-Eilot,S., Odeh,A., Kaganovsky,A. and Hasson,P.
  TITLE     Fibroblast diversification is an embryonic process dependent on
            muscle contraction
  JOURNAL   Cell Rep 43 (12), 115034 (2024)
   PUBMED   39636726
REFERENCE   2  (residues 1 to 498)
  AUTHORS   Hashimoto,K., Ohira,M., Kodama,A., Kimoto,M., Inoue,M., Tone,S.,
            Usui,Y., Hanashima,A., Goto,T., Ogura,Y., Ujihara,Y. and Mohri,S.
  TITLE     Loss of connectin novex-3 leads to heart dysfunction associated
            with impaired cardiomyocyte proliferation and abnormal nuclear
            mechanics
  JOURNAL   Sci Rep 14 (1), 13727 (2024)
   PUBMED   38877142
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 498)
  AUTHORS   Sun,Y., Lin,S., Wang,H., Wang,L., Qiu,Y., Zhang,F., Hao,N., Wang,F.
            and Tan,W.
  TITLE     Regulatory role of PI16 in autoimmune arthritis and intestinal
            inflammation: implications for Treg cell differentiation and
            function
  JOURNAL   J Transl Med 22 (1), 327 (2024)
   PUBMED   38566233
  REMARK    GeneRIF: Regulatory role of PI16 in autoimmune arthritis and
            intestinal inflammation: implications for Treg cell differentiation
            and function.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 498)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (residues 1 to 498)
  AUTHORS   Wang,F., Gu,X., Lin,S., Wu,Q., Sun,Y., Zhang,Q., Luo,A., Feng,X.,
            Wang,L., Xu,L., Sun,W. and Tan,W.
  TITLE     Peptidase inhibitor 16 promotes inflammatory arthritis by
            suppressing Foxp3 expression via regulating K48-linked ubiquitin
            degradation Bmi-1 in regulatory T cells
  JOURNAL   Clin Immunol 259, 109883 (2024)
   PUBMED   38147957
  REMARK    GeneRIF: Peptidase inhibitor 16 promotes inflammatory arthritis by
            suppressing Foxp3 expression via regulating K48-linked ubiquitin
            degradation Bmi-1 in regulatory T cells.
REFERENCE   6  (residues 1 to 498)
  AUTHORS   Angueira,A.R., Sakers,A.P., Holman,C.D., Cheng,L., Arbocco,M.N.,
            Shamsi,F., Lynes,M.D., Shrestha,R., Okada,C., Batmanov,K.,
            Susztak,K., Tseng,Y.H., Liaw,L. and Seale,P.
  TITLE     Defining the lineage of thermogenic perivascular adipose tissue
  JOURNAL   Nat Metab 3 (4), 469-484 (2021)
   PUBMED   33846639
REFERENCE   7  (residues 1 to 498)
  AUTHORS   Deng,M., Yang,S., Ji,Y., Lu,Y., Qiu,M., Sheng,Y., Sun,W. and
            Kong,X.
  TITLE     Overexpression of peptidase inhibitor 16 attenuates angiotensin
            II-induced cardiac fibrosis via regulating HDAC1 of cardiac
            fibroblasts
  JOURNAL   J Cell Mol Med 24 (9), 5249-5259 (2020)
   PUBMED   32227584
  REMARK    GeneRIF: Overexpression of peptidase inhibitor 16 attenuates
            angiotensin II-induced cardiac fibrosis via regulating HDAC1 of
            cardiac fibroblasts.
REFERENCE   8  (residues 1 to 498)
  AUTHORS   Singhmar,P., Trinh,R.T.P., Ma,J., Huo,X., Peng,B., Heijnen,C.J. and
            Kavelaars,A.
  TITLE     The fibroblast-derived protein PI16 controls neuropathic pain
  JOURNAL   Proc Natl Acad Sci U S A 117 (10), 5463-5471 (2020)
   PUBMED   32079726
  REMARK    GeneRIF: these findings support a model in which PI16 promotes
            neuropathic pain by mediating a cross-talk between fibroblasts and
            the endothelial barrier leading to barrier opening, cellular
            influx, and increased pain
REFERENCE   9  (residues 1 to 498)
  AUTHORS   Regn,M., Laggerbauer,B., Jentzsch,C., Ramanujam,D., Ahles,A.,
            Sichler,S., Calzada-Wack,J., Koenen,R.R., Braun,A., Nieswandt,B.
            and Engelhardt,S.
  TITLE     Peptidase inhibitor 16 is a membrane-tethered regulator of chemerin
            processing in the myocardium
  JOURNAL   J Mol Cell Cardiol 99, 57-64 (2016)
   PUBMED   27539859
  REMARK    GeneRIF: our data indicate that PI16 suppresses chemerin activation
            in the myocardium and suggest that this circuit may be part of the
            cardiac stress response.
REFERENCE   10 (residues 1 to 498)
  AUTHORS   Frost,R.J. and Engelhardt,S.
  TITLE     A secretion trap screen in yeast identifies protease inhibitor 16
            as a novel antihypertrophic protein secreted from the heart
  JOURNAL   Circulation 116 (16), 1768-1775 (2007)
   PUBMED   17909105
  REMARK    GeneRIF: PI16, a novel protein secreted from the heart, is strongly
            upregulated early in heart failure and inhibits growth of
            cardiomyocytes both in vitro and in vivo
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK157854.1, AI587910.1 and AB046537.1.
            
            On Oct 13, 2006 this sequence version replaced NP_076223.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB046537.1, BC039124.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 15.22 cM"
     Protein         1..498
                     /product="peptidase inhibitor 16 precursor"
                     /note="protease inhibitor 16; cysteine-rich protease
                     inhibitor"
                     /calculated_mol_wt=50624
     sig_peptide     1..29
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=3044
     mat_peptide     30..498
                     /product="Peptidase inhibitor 16. /id=PRO_0000287634"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET66.2)"
                     /calculated_mol_wt=50624
     Region          35..168
                     /region_name="CAP_PI16_HrTT-1"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of peptidase
                     inhibitor 16 and HrTT-1 protein; cd05559"
                     /db_xref="CDD:349405"
     Site            116
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9ET66.2)"
     Region          <183..458
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          204..277
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET66.2)"
     Region          317..407
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET66.2)"
     Region          419..467
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET66.2)"
     Site            447
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9ET66.2)"
     CDS             1..498
                     /gene="Pi16"
                     /gene_synonym="1200009H11Rik; Cripi; PI-16"
                     /coded_by="NM_023734.3:334..1830"
                     /db_xref="CCDS:CCDS28595.2"
                     /db_xref="GeneID:74116"
                     /db_xref="MGI:MGI:1921366"
ORIGIN      
        1 mhgscspwvm lpppllllll liatgpttal tedekqtmvd lhnqyraqvs ppasdmlqmr
       61 wddelaafak ayaqkcvwgh nkergrrgen lfaitdegmd vplavgnwhe eheyynfsta
      121 tcdpnqmcgh ytqvvwskte rigcgshfce tlqgveeani hllvcnyepp gnvkgrkpyq
      181 egtpcsqcpl gyscenslce pmrnpekaqd spprvtevps trateapssr etgtpslats
      241 etlhfsvtkv sdslatessp avetkapssl ategpssmat eaqafvtevp lvsarhmqps
      301 vdegpvnflt sthipvpksm deeaskssat svspkkslyp kmsltesges vpqiqeeaep
      361 kdelsepeai lpeaeaapte aevelrepea espkaespea eaesplssea lvpvlpaqer
      421 ggqkaslehs ghpaspslpt fpsasgnatg grtlalqssw tgaenpekad wdlknsahvw
      481 gpflglllps llllagmv
//
