LOCUS       NM_133821               6123 bp    mRNA    linear   ROD 10-JUN-2024
DEFINITION  Mus musculus PH domain and leucine rich repeat protein phosphatase
            1 (Phlpp1), mRNA.
ACCESSION   NM_133821 XM_001475839 XM_129968
VERSION     NM_133821.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Arnold,K.M., Weaver,S.R., Zars,E.L., Tschumperlin,D.J. and
            Westendorf,J.J.
  TITLE     Inhibition of Phlpp1 preserves the mechanical integrity of
            articular cartilage in a murine model of post-traumatic
            osteoarthritis
  JOURNAL   Osteoarthritis Cartilage 32 (6), 680-689 (2024)
   PUBMED   38432607
  REMARK    GeneRIF: Inhibition of Phlpp1 preserves the mechanical integrity of
            articular cartilage in a murine model of post-traumatic
            osteoarthritis.
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6123)
  AUTHORS   Qiu,Y., Meng,Y., Jia,Y., Lang,X., Zhao,H., Ding,L., Wang,T., Sun,H.
            and Gao,S.
  TITLE     Hyperglycemia-Induced Overexpression of PH Domain Leucine-Rich
            Repeat Protein Phosphatase 1 (PHLPP1) Compromises the
            Cardioprotective Effect of Ischemic Postconditioning Via Modulation
            of the Akt/Mst1 Pathway Signaling
  JOURNAL   Cardiovasc Drugs Ther 37 (6), 1087-1101 (2023)
   PUBMED   35715527
  REMARK    GeneRIF: Hyperglycemia-Induced Overexpression of PH Domain
            Leucine-Rich Repeat Protein Phosphatase 1 (PHLPP1) Compromises the
            Cardioprotective Effect of Ischemic Postconditioning Via Modulation
            of the Akt/Mst1 Pathway Signaling.
REFERENCE   4  (bases 1 to 6123)
  AUTHORS   Liao,Y., Peng,X., Li,X., Wu,D., Qiu,S., Tang,X. and Zhang,D.
  TITLE     CircRNA_45478 promotes ischemic AKI by targeting the
            miR-190a-5p/PHLPP1 axis
  JOURNAL   FASEB J 36 (12), e22633 (2022)
   PUBMED   36315192
  REMARK    GeneRIF: CircRNA_45478 promotes ischemic AKI by targeting the
            miR-190a-5p/PHLPP1 axis.
REFERENCE   5  (bases 1 to 6123)
  AUTHORS   Sharma,M. and Dey,C.S.
  TITLE     PHLPP isoforms differentially regulate Akt isoforms and AS160
            affecting neuronal insulin signaling and insulin resistance via
            Scribble
  JOURNAL   Cell Commun Signal 20 (1), 179 (2022)
   PUBMED   36376971
  REMARK    GeneRIF: PHLPP isoforms differentially regulate Akt isoforms and
            AS160 affecting neuronal insulin signaling and insulin resistance
            via Scribble.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6123)
  AUTHORS   Masubuchi,S., Gao,T., O'Neill,A., Eckel-Mahan,K., Newton,A.C. and
            Sassone-Corsi,P.
  TITLE     Protein phosphatase PHLPP1 controls the light-induced resetting of
            the circadian clock
  JOURNAL   Proc Natl Acad Sci U S A 107 (4), 1642-1647 (2010)
   PUBMED   20080691
  REMARK    GeneRIF: show that PHLPP1-null mice, although displaying normal
            circadian rhythmicity, have a drastically impaired capacity to
            stabilize the circadian period after light-induced resetting,
            producing a large phase shift after light resetting.
REFERENCE   7  (bases 1 to 6123)
  AUTHORS   Shimizu,K., Phan,T., Mansuy,I.M. and Storm,D.R.
  TITLE     Proteolytic degradation of SCOP in the hippocampus contributes to
            activation of MAP kinase and memory
  JOURNAL   Cell 128 (6), 1219-1229 (2007)
   PUBMED   17382888
  REMARK    GeneRIF: To determine if hippocampus-dependent memory is influenced
            by SCOP in vivo, a transgenic mouse strain was generated for the
            inducible overexpression of SCOP in the forebrain; overexpression
            SCOP completely blocked memory for novel objects.
REFERENCE   8  (bases 1 to 6123)
  AUTHORS   Yamamoto,H., Imai,K., Takamatsu,Y., Kamegaya,E., Hara,Y.,
            Shimada,K., Yamamoto,T., Shen,H.W., Hagino,Y., Kobayashi,H.,
            Ide,S., Sora,I., Koga,H. and Ikedaa,K.
  TITLE     Changes in expression of the mouse homologues of KIAA genes after
            subchronic methamphetamine treatment
  JOURNAL   Ann N Y Acad Sci 1025, 92-101 (2004)
   PUBMED   15542705
REFERENCE   9  (bases 1 to 6123)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 6123)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Hara,Y., Nagase,T.,
            Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: I. The complete nucleotide sequences of 100 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 9 (5), 179-188 (2002)
   PUBMED   12465718
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124710.4, BC059254.1, CN525246.1 and CF535385.1.
            
            On Sep 9, 2009 this sequence version replaced NM_133821.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB093251.1, SRR10223971.732.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1407              AC124710.4         79338-80744
            1408-1643           BC059254.1         69-304
            1644-2246           CN525246.1         110-712
            2247-2305           BC059254.1         907-965
            2306-2711           CF535385.1         303-708
            2712-6123           BC059254.1         1372-4783
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 49.76 cM"
     gene            1..6123
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /note="PH domain and leucine rich repeat protein
                     phosphatase 1"
                     /db_xref="GeneID:98432"
                     /db_xref="MGI:MGI:2138327"
     exon            1..1579
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..5199
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /EC_number="3.1.3.16"
                     /note="pleckstrin homology domain containing, family E
                     (with leucine rich repeats) member 1; suprachiasmatic
                     nucleus circadian oscillatory protein; PH
                     domain-containing family E member 1; pleckstrin homology
                     domain-containing family E member 1"
                     /codon_start=1
                     /product="PH domain leucine-rich repeat-containing protein
                     phosphatase 1"
                     /protein_id="NP_598582.3"
                     /db_xref="CCDS:CCDS48337.1"
                     /db_xref="GeneID:98432"
                     /db_xref="MGI:MGI:2138327"
                     /translation="MEPAAAAPAQRLADPTGEDQALAAAAAEGGRCPDPALSAAAPSG
                     GNGGAAREEAPCEAPPGPLPGRAGGTGRRRRRGAPQPAAGGAAPVPAAGGGANSLLLK
                     RGRLKRNLSAAAAASSSSSPSSASSAAGGLPASCSASASLCTRSLDRKTLLLKHRQLL
                     QLQPSDRDWVRHQLQRGCVHVFDRHMASSYLRPVLCTLDTTAAEVAARLLQLGHKGGG
                     VVKVLGYGPPPAAAPAASDQTLDGEHGRDVEPPPSSGTVGAVRGPARAPPADLPLPGG
                     AWTRCAPRISPAPSDSSPGELFAGGPGSPPRAPRPASDTESFSLSPSAESVSDRLDPY
                     SSGGGGSSSSSEELEADPAMPHRPGRPAQPRPPSPKTSALLQPKAPTGVDSTGVIAGE
                     GPGDDKAMAAAAPDVPLSTSGRIRETVQKTSPPSLYVQLHGETTRRLEADEKPLQIQN
                     DYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQL
                     PVNRWTRRQVILCGTCLIVSSVKDSVSGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQ
                     SQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLK
                     QNFLRQTPTLPAARGLGELQRFTKLKSLNLSNNHLGAFPSAVCSIPTLAELNVSCNAL
                     REVPAAVGDMQNLQTFLLDGNFLQSLPAELESMHQLSYLGLSFNEFTDIPEVLEKLTA
                     VDKLCMAGNCVETLRLQALRRMPHIKHVDLRLNILRKLMADEVDFVQHVTQLDLRDNK
                     LGDLDAMIFNNIEVLHCERNQLVTLNVCGYFLKALYASSNELAQLDVYPVPNYLSYMD
                     VSRNCLESVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNRLARLPER
                     LERTSVEVLDVQHNQITELPPNLLMKADSLRFLNASANKLETLPPATLSEETSSILQE
                     LYLTNNCLTDKCVPLLTGHPRLKILHMAYNRLQSFPASKMAKLEELEEIDISGNKLKA
                     IPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEVKCVDLSCNELSEITLPENLPPKLQE
                     LDLTGNPRLALDHKSLELLNNIRCFKIDQPSAGDASGAPAVWSHGYTEASGVKNKLCV
                     AALSVNNFRDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFI
                     VMQRKLGTAGQKLGGAAVLCHIKPDPVDLGGSFTLTSANVGKCQTVLCRNGKPLSLSR
                     SYIMSCEEERKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTP
                     QDEFFILGSKGLWDSLSIDEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQL
                     SVTEDSFCCCELSAGGSMPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSE
                     LSTSEMSSEVGSTASDEPPSGVLNESSPAYPNEQRCMLHPVCLSNSFQRQLSSATFSS
                     AFSDNGLDSDDEEPIEGVFSNGSRVEVEVDIHCSRAKEKERQQHLLQVPAEASDEGIV
                     ISANEDESGLSKKADFSAVGTIGRRRANGSVAPQERSHNVIEVAADAPLRKPGGYFAA
                     PAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQQQQLPPPPQPPQPQPQPQPQPQP
                     QPQRHFQMDHLPDCYDTPL"
     exon            1580..1776
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1902
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..2069
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2216
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2447
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2448..2650
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2651..2711
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2712..2807
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..2963
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2964..3164
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3165..3327
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3328..3458
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3459..3563
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3564..3758
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3759..3987
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3988..6104
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6082..6087
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /note="hexamer: AATAAA"
     polyA_site      6104
                     /gene="Phlpp1"
                     /gene_synonym="mKIAA0606; Phlpp; Plekhe1; SCOP"
                     /note="major polyA site"
ORIGIN      
        1 tggagcggcc gcgccgaacg ccatcggcgt ctccgttctc ttcgcgccgc cgccgccgcc
       61 gtctcccacc ttcgcctcac ctcctcctcc tccgctgcct ccggagctgg gggagcgacg
      121 tgaagccccg ctacgatgga gcccgccgct gcagccccgg cacagcgact cgcggacccc
      181 acgggtgagg accaagccct ggcggcggcg gcggccgagg gcggccggtg tccggatccc
      241 gcgctgagcg cggcggcccc gagcggcggg aacggcggcg cggcgcgaga ggaggccccc
      301 tgcgaggcgc cgccggggcc gctgcccggc cgcgccggag gcaccgggcg caggcggcgg
      361 cggggagctc cccagcccgc ggccggtggg gccgctccgg tgcccgcggc cggcggcggc
      421 gccaactcgc tgctgctgaa gagagggcgg ctgaagagga atctgtcggc cgccgccgcc
      481 gcctcgtcgt cctcgtcgcc gtcctcggcg tcttcggccg ctggcggcct cccggcgtcc
      541 tgctcggcct cggcgtcgct gtgcacccgg agcctggaca ggaagacgct gctcctgaag
      601 caccggcagc tgcttcagct gcagccgtcg gacagggact gggtgaggca ccagctccag
      661 cggggctgtg tgcacgtctt tgaccgccac atggcctcgt cctacctgcg cccagtgctc
      721 tgcacgctgg acaccacggc cgccgaggtg gcggcccggc ttctgcagct gggccacaaa
      781 ggcggcgggg tggtgaaggt gctgggctac gggccgcctc ccgccgccgc ccctgccgcc
      841 agcgatcaga ccctggacgg agagcacgga agggacgtgg agccaccgcc ctcgagcggc
      901 actgttggtg ctgtccgggg ccctgcgcgc gcgcctcccg ccgatctgcc gctgcccggg
      961 ggcgcctgga cgcgctgtgc accccgcatc agccccgcgc cttcggactc cagccccgga
     1021 gagctgttcg cgggcggccc cgggtcccct ccccgcgccc cgcgcccggc ctctgacacc
     1081 gagagcttca gcctgagccc cagcgccgag agcgtgtcag accggctgga cccctacagc
     1141 agcggcggcg gcggctcctc gtcgtcgtcg gaggagttag aggctgatcc ggccatgccc
     1201 caccgccccg gccgccccgc gcagcctcgg ccgccttccc cgaagacctc ggctttactg
     1261 cagccgaaag ccccgacggg cgtagacagc actggcgtga tagcgggcga gggtcccgga
     1321 gacgataagg cgatggcagc cgcagctccg gacgttcctc tgtcgacttc ggggaggata
     1381 cgggagaccg tgcaaaagac atctccgccc tccctgtatg tacagctcca tggagagact
     1441 acccggcgcc tggaggcgga cgagaagcca ttgcagatcc aaaatgacta cctcttccaa
     1501 ctgggatttg gggagctgtg gagggtgcag gaggaaggca tggactcaga gattggctgt
     1561 ctcattcgat tctatgcagg aaagcctcat agcactggga gctctgagcg gattcagctc
     1621 tccggaatgt acaatgtccg aaaaggcaaa atgcagttgc cagtgaaccg atggacaaga
     1681 cgacaggtca ttctgtgcgg gacgtgcttg atagtgtcgt ccgtgaaaga cagcgtgagt
     1741 gggaagatgc atgtcctgcc cctcattggt ggaaaagtgg aggaagtgaa aaagcaccag
     1801 cactgtttag cgttcagctc ttcggggcct caaagccaga cttactacat ctgctttgat
     1861 actttcacgg agtacttacg gtggctgcgg caagtctcca aggttgcatc acagcgtata
     1921 agctcagtag acctctcctg ttgtagcctt gaacatctgc ctgccaacct cttttacagc
     1981 caagacctta ctcatctcaa cttaaaacag aacttcctaa ggcaaacccc caccctccca
     2041 gctgccagag gacttggtga actgcaaagg ttcaccaaat tgaagagcct taacctttcc
     2101 aataaccacc taggagcctt tccgtcagca gtctgcagca ttccaaccct agcagagctg
     2161 aatgtatctt gcaatgccct gcgagaagtc ccagcagctg ttggagatat gcagaacttg
     2221 cagacgtttc tgctggatgg aaattttctc cagtcccttc ctgctgaact ggagagcatg
     2281 caccagctca gctatctggg tctttccttt aatgaattca ctgacattcc agaggtattg
     2341 gagaagctga ctgctgtgga taagctgtgt atggctggga actgtgtgga gaccctcaga
     2401 ctacaggcct taagaagaat gcctcatatt aaacatgtgg acctaagact gaacatactc
     2461 agaaagctta tggcagatga ggtggacttt gtgcaacatg tcactcagct tgacctgcga
     2521 gacaataagc ttggtgatct agatgctatg atcttcaaca acatagaagt tctgcactgt
     2581 gagaggaatc agctggtgac attgaacgtt tgtggctatt tcctaaaggc actctatgct
     2641 tcttctaatg aacttgctca acttgatgtc tacccagttc cgaattatct ctcttacatg
     2701 gatgtctcaa gaaactgcct agaaagtgtg cctgagtggg tatgtgaaag ccggaaatta
     2761 gaagttttgg atattggcca taatcaaata tgtgaacttc ctgcccgcct gttttgtaat
     2821 agtagtctcc ggaaattgct agcaggacac aaccggttgg caaggcttcc tgaaaggctg
     2881 gagagaactt ctgtggaggt cttggatgtc cagcacaacc agatcactga gctcccacca
     2941 aacctcctca tgaaggctga cagcctgaga ttcctgaatg catctgcaaa caaactggaa
     3001 accctgcctc cagccacact ttctgaggag acgagcagta tattacagga gttgtacctg
     3061 acaaacaact gcctcacgga taagtgtgtg cccttgttaa cagggcaccc ccgtctgaag
     3121 atcctacaca tggcctataa ccggcttcag agcttcccgg caagtaaaat ggcaaaactg
     3181 gaggaacttg aagaaattga tatcagtggg aataagctga aagccatccc cacaacaatc
     3241 atgaactgca gacgcatgca caccgtgatt gctcactcca attgtatcga agtctttcct
     3301 gaagttatgc agctcccaga agtcaagtgt gtagatctga gctgtaatga gctgagtgaa
     3361 atcacgttac cagaaaacct gccacctaag ctgcaggagc tagacctgac tggaaatcca
     3421 cgccttgccc tggaccacaa aagcctggag ctgctcaata acattcgctg cttcaagatt
     3481 gaccagccgt cagcaggaga tgcatcagga gccccagcag tatggagtca tggttacact
     3541 gaagcatcgg gagtaaaaaa caagctgtgt gtcgcagccc tgtctgtaaa taacttccgt
     3601 gacaaccgag aggccctcta tggtgtgttt gatggagacc ggaatgtgga ggtgccctac
     3661 cttctccagt gcaccatgag tgacattttg gctgaagagc ttcagaaaac gaaaaatgaa
     3721 gaagaataca tggtcaatac attcatagtc atgcaaagga aactgggaac tgctgggcag
     3781 aaacttggtg gtgctgctgt cctctgccac atcaagcctg accctgtgga cctgggagga
     3841 tcctttaccc tgacctctgc taatgttggc aagtgccaaa cggttctctg tcgaaatggg
     3901 aagccactgt ctctgtccag gtcatacatc atgagctgtg aagaagagcg gaagaggatt
     3961 aagcagcaca aagccatcat cactgaggat ggcaaggtca atggagtgac agagtccacg
     4021 cgcatcctgg gctacacctt cctccatccc agtgtggtgc ctcgccccca tgtgcagtcg
     4081 gtgcttctga ctccacagga tgagtttttc atcctgggca gcaaagggct gtgggacagc
     4141 ctatccattg acgaggctgt ggaagctgta cgcaatgtgc cagatgctct ggctgctgct
     4201 aagaagctct gcaccctggc tcagagctat ggctgccatg acagcatcag tgctgtggtg
     4261 gtacagctaa gtgtcaccga agacagcttc tgctgctgtg agctcagtgc tggagggagc
     4321 atgccaccac ccagccccgg aatcttccca ccatccgtca acatggtcat caaggaccga
     4381 ccctcagatg ggctgggtgt gccatcctcc agcagtggca tggcatctga gatcagcagt
     4441 gaactgtcta cctccgagat gagtagtgag gtgggctcca cagcctctga tgagcccccg
     4501 tctggagtcc tgaatgagag cagccctgcc taccccaacg agcagcgctg catgctccac
     4561 cctgtctgcc tgtctaactc cttccaacgt cagctgtcca gcgctacttt ctccagcgcg
     4621 ttctctgaca acggccttga cagtgacgat gaagaaccca ttgagggggt gttcagcaac
     4681 ggcagccggg ttgaggtgga agtagacatc cactgcagca gggccaagga aaaggagaga
     4741 cagcagcacc tacttcaggt gccagctgag gccagtgatg agggcattgt catcagtgcc
     4801 aatgaggatg agtcaggtct gtccaagaag gcagactttt ctgctgtggg aaccattgga
     4861 cgacgcaggg ctaatggctc tgtagctccc caggaaagga gccataatgt gatagaggtt
     4921 gctgcagatg cgcctctccg gaagccagga ggctattttg cagcccctgc tcagccagat
     4981 ccagatgatc agtttatcat ccccccagag ctggaagagg aagtcaaaga aatcatgaaa
     5041 catcaccagg agcagcagca gcagcagcag cagcagcagc tgccaccacc accccagccg
     5101 ccacagccac agccacagcc acagccacag ccacagccac agccacagcg gcacttccaa
     5161 atggatcacc tgccagactg ttacgataca ccgctatgac ccagcctgag tactgtttta
     5221 aacaataaac taaccagaga gactgagagt ctcccaggct cacattaaac caggggtgtt
     5281 aacttcatgc cctctcccag acactgcccc acctgtctct gcagctcatc tgtaggttct
     5341 ctttgtattg gttactttta acaattaacc acttttcttc tagtgatact ttaccaatat
     5401 gatttaaatt tgttaacttc tttccctaac atatcaactg tgtaaagaca aagaacaaaa
     5461 ggtttaatat attacagaga aacagttaat gataatgtaa tatttttaaa aatggctttt
     5521 gttgttttgt ttgtttggaa ggcagggcaa gttgccattg ctaaatgatt taataatatt
     5581 gtaattgtgt atttctttgt ggggaaagct tttttttgtc ttgaaaatgg tagacatttg
     5641 tagaatatgg cgactaactc ctaggagttg ctttactctg tcaggtgact taagtcactg
     5701 ggagtcacta attttctctg agagaacagt tgattgagaa atttctattg taaatatctc
     5761 agtgttacat agtgaagctt aaagtgtttg tagtcttgca taaggacaca gcctaaataa
     5821 ctgacccctt actcgctctg aagagcctcc ctgccccacc cctacgtagc tcatcttcag
     5881 tgtgggccat gtgcagcgtg tttctgtgca gataatgtgg ccctgttctc gggaacccag
     5941 aaccttttac agacattttt taattgtgtt ccttcctgct gccacaatca gcatgactgt
     6001 atagagtccc ttataccatc tacacatcca gagttatatg gaagcagaat gcccaaatgg
     6061 acatgtcgta atttttgtta taataaatat gaaatttaca agtaaaaaaa aaaaaaaaaa
     6121 aaa
//
