LOCUS       NP_700461               1249 aa            linear   ROD 28-APR-2025
DEFINITION  pleckstrin homology-like domain family B member 2 isoform 2 [Mus
            musculus].
ACCESSION   NP_700461 XP_489563
VERSION     NP_700461.2
DBSOURCE    REFSEQ: accession NM_153412.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1249)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 1249)
  AUTHORS   Xie,M.J., Yagi,H., Iguchi,T., Yamazaki,H., Hanamura,K.,
            Matsuzaki,H., Shirao,T. and Sato,M.
  TITLE     Phldb2 is essential for regulating hippocampal dendritic spine
            morphology through drebrin in an adult-type isoform-specific manner
  JOURNAL   Neurosci Res 185, 1-10 (2022)
   PUBMED   36162735
  REMARK    GeneRIF: Phldb2 is essential for regulating hippocampal dendritic
            spine morphology through drebrin in an adult-type isoform-specific
            manner.
REFERENCE   3  (residues 1 to 1249)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 1249)
  AUTHORS   Xie,M.J., Ishikawa,Y., Yagi,H., Iguchi,T., Oka,Y., Kuroda,K.,
            Iwata,K., Kiyonari,H., Matsuda,S., Matsuzaki,H., Yuzaki,M.,
            Fukazawa,Y. and Sato,M.
  TITLE     PIP3-Phldb2 is crucial for LTP regulating synaptic NMDA and AMPA
            receptor density and PSD95 turnover
  JOURNAL   Sci Rep 9 (1), 4305 (2019)
   PUBMED   30867511
  REMARK    GeneRIF: PIP3-Phldb2 is crucial for LTP regulating synaptic NMDA
            and AMPA receptor density and PSD95 turnover.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 1249)
  AUTHORS   Lim,B.C., Matsumoto,S., Yamamoto,H., Mizuno,H., Kikuta,J., Ishii,M.
            and Kikuchi,A.
  TITLE     Prickle1 promotes focal adhesion disassembly in cooperation with
            the CLASP-LL5beta complex in migrating cells
  JOURNAL   J Cell Sci 129 (16), 3115-3129 (2016)
   PUBMED   27378169
REFERENCE   6  (residues 1 to 1249)
  AUTHORS   Basu,S., Sladecek,S., Martinez de la Pena y Valenzuela,I.,
            Akaaboune,M., Smal,I., Martin,K., Galjart,N. and Brenner,H.R.
  TITLE     CLASP2-dependent microtubule capture at the neuromuscular junction
            membrane requires LL5beta and actin for focal delivery of
            acetylcholine receptor vesicles
  JOURNAL   Mol Biol Cell 26 (5), 938-951 (2015)
   PUBMED   25589673
  REMARK    GeneRIF: A transport system, organized by agrin through PI3 kinase,
            GSK3beta, CLASP2, and LL5beta, for precise delivery of
            acetylcholine receptor vesicles from subsynaptic nuclei to
            overlying synaptic membrane was identified at neuromuscular
            junction membrane.
REFERENCE   7  (residues 1 to 1249)
  AUTHORS   Hiepen,C., Benn,A., Denkis,A., Lukonin,I., Weise,C.,
            Boergermann,J.H. and Knaus,P.
  TITLE     BMP2-induced chemotaxis requires PI3K p55gamma/p110alpha-dependent
            phosphatidylinositol (3,4,5)-triphosphate production and LL5beta
            recruitment at the cytocortex
  JOURNAL   BMC Biol 12, 43 (2014)
   PUBMED   24885555
  REMARK    GeneRIF: These results provide evidence for an important
            contribution of the BMP2-PI3K (p55gamma/p110alpha)- PIP3-LL5beta
            signalling axis in mesenchymal progenitor cell chemotaxis.
            Publication Status: Online-Only
REFERENCE   8  (residues 1 to 1249)
  AUTHORS   Kishi,M., Kummer,T.T., Eglen,S.J. and Sanes,J.R.
  TITLE     LL5beta: a regulator of postsynaptic differentiation identified in
            a screen for synaptically enriched transcripts at the neuromuscular
            junction
  JOURNAL   J Cell Biol 169 (2), 355-366 (2005)
   PUBMED   15851520
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164979.2 and AC166572.1.
            
            On May 18, 2008 this sequence version replaced NP_700461.1.
            
            Transcript Variant: This variant (2) lacks an exon and uses an
            alternate splice site in the coding region, but maintains the
            reading frame, compared to variant 1. The encoded isoform (2) is
            shorter than isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF506821.1, SRR10223970.18611.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 30.08 cM"
     Protein         1..1249
                     /product="pleckstrin homology-like domain family B member
                     2 isoform 2"
                     /note="protein LL5-beta"
                     /calculated_mol_wt=141355
     Region          64..85
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            71
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q86SQ0; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            73
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q86SQ0; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Region          128..154
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            156
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q86SQ0; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Region          190..248
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            203
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            241
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            244
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Region          264..289
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            329
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            333
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            347
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            380
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            383
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Region          388..424
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            389
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            411
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q86SQ0; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            416
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q86SQ0; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            465
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:19131326,
                     ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            486
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            510
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17242355,
                     ECO:0007744|PubMed:19131326, ECO:0007744|PubMed:21183079;
                     propagated from UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            546
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:17242355,
                     ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            570
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q86SQ0; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Region          <577..>800
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          866..934
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Site            894
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q86SQ0; propagated from
                     UniProtKB/Swiss-Prot (Q8K1N2.2)"
     Region          <1030..>1097
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     Region          1138..1242
                     /region_name="PH_PHLDB1_2"
                     /note="Pleckstrin homology-like domain-containing family B
                     member 2 pleckstrin homology (PH) domain; cd14673"
                     /db_xref="CDD:270192"
     CDS             1..1249
                     /gene="Phldb2"
                     /gene_synonym="C820004H04Rik; LL5b; LL5beta"
                     /coded_by="NM_153412.4:186..3935"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /db_xref="CCDS:CCDS28202.1"
                     /db_xref="GeneID:208177"
                     /db_xref="MGI:MGI:2444981"
ORIGIN      
        1 maedshmqkq lefqngslee gfvvrslene pqnmmeslsp rkyssslkfk angdysgsyl
       61 tlsqpvsakr spspmgtsvr sspslakiqg skqfcdgidk nismkppisf lssaaslggy
      121 plgkadldhy tgrdserstr lsekppysry ssrnkshdsv yflgglegrk tsgslltmwn
      181 gnslsctgss pisrsgaasm psspkqvrkm nlqdhstlqp rlsrhkepas envsvrtrky
      241 sgsslsnmga ysrslprlyk atdnqmspls lpprsslgns rrgqlgekdl phslvdsdny
      301 lnfsslssga spyktclseg npyvssalsv pasprvarkm llastssddf drasysgtsp
      361 shsfisgepd rvlvarrnfs cgsmelddsd leslrqsset pqpvlrerks sissisgrdd
      421 lmdyhrrqre erlreqemer lerqrletil slcaeytkpe grrlsagttv advqkinkel
      481 eklqlsdees vfedalvcpd aryrchrkgs lqdvdvagfg nlghsasfla prgsrsdell
      541 gdltrtppss saaflkatne ssylsilpkt pedigeeqrt qelaamedar mvilnnleel
      601 eqkikdindq mdessreldm ecalldgeqk setaelmkek eildhlnrki teleknivge
      661 ktkekvklda ereklerlqe lyseqktqld ncpesmreql qqqlkrdadl ldveskhfed
      721 lefqqlehes rldeekenlt qqllrevaey qrnivarkek isalkkqash ivqqaqreqd
      781 hfvkeknnli mmlqrekenl cnlekkyssl tggkgfpinp ntlkegyisv neinescgns
      841 tnlspstqfp adadaavtep alavpvsqpq ssehfrslee rkkqhkegly lsdtlprkkt
      901 tpslsphfss atmgrsttpk ahlplgqsns cgsvlphsla tmtkdsesrr mlrgynhqqm
      961 segqrqkpef ysrtasesnv ylnsfhypdr sykdqaydtl sldssdsmet sisacspdni
     1021 ssastsniar ieemerllkq ahaektrlle sreremeakk raleeekrrr eilekrlqee
     1081 tsqrqkliek evkirekqra qarpltrylp vrkedfdlrs hvetaghnid tcfhvsitek
     1141 tcrgylikmg gkiktwkkrw fvfdrnkrtf syyadkheak lkgviyfqai eevyydhlkn
     1201 ankspnpllt fsvkthdriy ymvapspeam riwmdvivtg aegythfll
//
