LOCUS       NM_013750               1483 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus pleckstrin homology like domain, family A, member 3
            (Phlda3), mRNA.
ACCESSION   NM_013750
VERSION     NM_013750.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Hasapis,S., Caraballo,I., Sears,T.J., Brock,K.D., Cart,J.B.,
            Moding,E.J. and Lee,C.L.
  TITLE     Characterizing the role of Phlda3 in the development of acute
            toxicity and malignant transformation of hematopoietic cells
            induced by total-body irradiation in mice
  JOURNAL   Sci Rep 13 (1), 12916 (2023)
   PUBMED   37558703
  REMARK    GeneRIF: Characterizing the role of Phlda3 in the development of
            acute toxicity and malignant transformation of hematopoietic cells
            induced by total-body irradiation in mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Chen,S., Lin,X., He,R., Zhang,W., Kang,M. and Xu,R.
  TITLE     PHLDA3 activated by BARX2 transcription, suppresses the malignant
            development of esophageal squamous cell carcinoma by downregulating
            PI3K/AKT levels
  JOURNAL   Exp Cell Res 426 (2), 113567 (2023)
   PUBMED   36965748
  REMARK    GeneRIF: PHLDA3 activated by BARX2 transcription, suppresses the
            malignant development of esophageal squamous cell carcinoma by
            downregulating PI3K/AKT levels.
REFERENCE   3  (bases 1 to 1483)
  AUTHORS   Beauchamp,M.C., Boucher,A., Dong,Y., Aber,R. and
            Jerome-Majewska,L.A.
  TITLE     Craniofacial Defects in Embryos with Homozygous Deletion of Eftud2
            in Their Neural Crest Cells Are Not Rescued by Trp53 Deletion
  JOURNAL   Int J Mol Sci 23 (16), 9033 (2022)
   PUBMED   36012294
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1483)
  AUTHORS   Alam,S.S., Kumar,S., Beauchamp,M.C., Bareke,E., Boucher,A.,
            Nzirorera,N., Dong,Y., Padilla,R., Zhang,S.J., Majewski,J. and
            Jerome-Majewska,L.A.
  TITLE     Snrpb is required in murine neural crest cells for proper splicing
            and craniofacial morphogenesis
  JOURNAL   Dis Model Mech 15 (6) (2022)
   PUBMED   35593225
REFERENCE   5  (bases 1 to 1483)
  AUTHORS   Ohki,R., Saito,K., Chen,Y., Kawase,T., Hiraoka,N., Saigawa,R.,
            Minegishi,M., Aita,Y., Yanai,G., Shimizu,H., Yachida,S., Sakata,N.,
            Doi,R., Kosuge,T., Shimada,K., Tycko,B., Tsukada,T., Kanai,Y.,
            Sumi,S., Namiki,H., Taya,Y., Shibata,T. and Nakagama,H.
  TITLE     PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine
            tumors
  JOURNAL   Proc Natl Acad Sci U S A 111 (23), E2404-E2413 (2014)
   PUBMED   24912192
  REMARK    GeneRIF: Data indicate that tumor suppressor PHLDA3-deficient mice
            develop islet hyperplasia.
REFERENCE   6  (bases 1 to 1483)
  AUTHORS   Wang,D., Zhang,Z., O'Loughlin,E., Wang,L., Fan,X., Lai,E.C. and
            Yi,R.
  TITLE     MicroRNA-205 controls neonatal expansion of skin stem cells by
            modulating the PI(3)K pathway
  JOURNAL   Nat Cell Biol 15 (10), 1153-1163 (2013)
   PUBMED   23974039
REFERENCE   7  (bases 1 to 1483)
  AUTHORS   Gineste,C., De Winter,J.M., Kohl,C., Witt,C.C., Giannesini,B.,
            Brohm,K., Le Fur,Y., Gretz,N., Vilmen,C., Pecchi,E., Jubeau,M.,
            Cozzone,P.J., Stienen,G.J., Granzier,H., Labeit,S.,
            Ottenheijm,C.A., Bendahan,D. and Gondin,J.
  TITLE     In vivo and in vitro investigations of heterozygous nebulin
            knock-out mice disclose a mild skeletal muscle phenotype
  JOURNAL   Neuromuscul Disord 23 (4), 357-369 (2013)
   PUBMED   23375831
REFERENCE   8  (bases 1 to 1483)
  AUTHORS   Saxena,A., Morozov,P., Frank,D., Musalo,R., Lemmon,M.A.,
            Skolnik,E.Y. and Tycko,B.
  TITLE     Phosphoinositide binding by the pleckstrin homology domains of Ipl
            and Tih1
  JOURNAL   J Biol Chem 277 (51), 49935-49944 (2002)
   PUBMED   12374806
  REMARK    GeneRIF: Ipl and Tih1 are bona fide PH domain proteins, with broad
            specificity and moderate affinity for PIPs.
REFERENCE   9  (bases 1 to 1483)
  AUTHORS   Frank,D., Fortino,W., Clark,L., Musalo,R., Wang,W., Saxena,A.,
            Li,C.M., Reik,W., Ludwig,T. and Tycko,B.
  TITLE     Placental overgrowth in mice lacking the imprinted gene Ipl
  JOURNAL   Proc Natl Acad Sci U S A 99 (11), 7490-7495 (2002)
   PUBMED   12032310
REFERENCE   10 (bases 1 to 1483)
  AUTHORS   Frank,D., Mendelsohn,C.L., Ciccone,E., Svensson,K., Ohlsson,R. and
            Tycko,B.
  TITLE     A novel pleckstrin homology-related gene family defined by
            Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression,
            chromosomal location, and parental imprinting
  JOURNAL   Mamm Genome 10 (12), 1150-1159 (1999)
   PUBMED   10594239
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC162441.11, AK003767.1 and AI834921.1.
            
            On Jun 23, 2009 this sequence version replaced NM_013750.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            CCDS Note: This CCDS ID represents the protein described in PMIDs:
            12374806 and 10594239 and is supported by AK003767.1. It should be
            noted this transcript is predicted to undergo nonsense-mediated
            mRNA decay (NMD). However, the protein is represented because it
            was detected endogenously in PMID: 10594239. It is likely that the
            majority of transcripts representing this variant will undergo NMD,
            while some low level of NMD escape may allow for the expression of
            this protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC023408.1, AK003767.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            NMD candidate          :: PMID: 10594239
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AC162441.11        111859-111892       c
            35-1466             AK003767.1         1-1432
            1467-1483           AI834921.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 59.26 cM"
     gene            1..1483
                     /gene="Phlda3"
                     /gene_synonym="Tih1"
                     /note="pleckstrin homology like domain, family A, member
                     3"
                     /db_xref="GeneID:27280"
                     /db_xref="MGI:MGI:1351485"
     exon            1..810
                     /gene="Phlda3"
                     /gene_synonym="Tih1"
                     /inference="alignment:Splign:2.1.0"
     CDS             365..742
                     /gene="Phlda3"
                     /gene_synonym="Tih1"
                     /note="TDAG51/Ipl homolog 1; TDAG/lpl homolog 1"
                     /codon_start=1
                     /product="pleckstrin homology-like domain family A member
                     3"
                     /protein_id="NP_038778.1"
                     /db_xref="CCDS:CCDS35722.1"
                     /db_xref="GeneID:27280"
                     /db_xref="MGI:MGI:1351485"
                     /translation="MTAAATVLKEGVLEKRSGGLLQLWKRKRCVLTERGLQLFEAKGT
                     GGRPKELSFARIKAVECVESTGRHIYFTLVTEGGGEIDFRCPLEDPGWNAQITLGLVK
                     FKNQQAIQTVRARQSLGTGTLVS"
     exon            811..1467
                     /gene="Phlda3"
                     /gene_synonym="Tih1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1436..1441
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phlda3"
                     /gene_synonym="Tih1"
                     /note="hexamer: AATGAA"
     polyA_site      1467
                     /gene="Phlda3"
                     /gene_synonym="Tih1"
                     /note="major polyA site"
ORIGIN      
        1 ggggcaggaa tgcgcggcgg gcggcgcagg aggcgagcgg tggaacatgt aagggcacat
       61 cccgccagca gccgcccagc tcgcagacgg agcgcagggg agcgggcgtg cgggcggcga
      121 ggcgcagggc accgggtaga ggctcgggga ggtcgaagcg gcgcgcaggg ttccaagccg
      181 gcggcgagag agtagcatcg cacgagcctc ccggtacccg ctgtctgcga ggcggcccgg
      241 gccgcaggct cgaccgcaca gggagcaagg ccaaccagct ccagggcgcg agaagccggc
      301 gctgtagagc cgggacagcc gggatcgccg cagcctccag agctgtggga acctcggggt
      361 gcccatgacg gcggcggcga ccgtgctgaa ggagggcgtg ctggagaagc gcagcggcgg
      421 gctgctgcag ctgtggaagc ggaagcgctg cgtgctcacc gagcgcgggc tgcagctctt
      481 cgaggccaag ggcacgggcg gccggcccaa ggagctcagc ttcgcccgca tcaaagccgt
      541 ggagtgcgta gagagcaccg ggcgccacat ctacttcacg ctagtgaccg aaggcggggg
      601 cgagatcgac ttccgctgcc ccctcgaaga ccctggctgg aacgctcaga tcaccctggg
      661 cctggtcaag ttcaagaatc aacaggccat ccagactgtg cgggcccggc agagtcttgg
      721 gactgggacc cttgtgtcct aaaccatgag gcgtatcatt ttatcgacat gccccccctc
      781 tcccatctcc gtgcccagaa gacatgtcag cttctctgtc cactttggtt ggttgggacc
      841 tggctacatg tgatgagcca gaagctatca acatgaggaa gataaaccta tcatcatgga
      901 aggagccaac cagccagaga cggagagcag aagccagtag gggctgagca tgaagattca
      961 gttcctggac actaagactg ttgttatatc cagctcagac ttgcgagcca caggcctggc
     1021 ctccagtatc atgacttggg ccttacctgg tccagccctt ggttctgggt tctgcagact
     1081 gctgtttgac ctctggcttt gagacatgcc caaagaaggg ctggctgttc ttcatggcgt
     1141 gctaagccag tgcctcagaa ctcaggaggc cagcctgggg tccagaagat gaccacctta
     1201 ccttaggaca gccactggac tcagcttgtg gaggggggtc tgctgggctg gagtcctgtg
     1261 cctggggggg gtctcctgct gcttaggtcc ttttgggacc cccccgccca tctgtaccct
     1321 ttctttgcac acttcttccc tcacccctgt tgccctctcc tcacttcggt gttgggcctg
     1381 gagggggtgg gggtggggta agggttggcc ctttaccatt tcatgcttat tccagaatga
     1441 agaggcccag ttcgtgggca gtaaccaaaa aaaaaaaaaa aaa
//
