LOCUS       NM_001403921            8134 bp    mRNA    linear   ROD 01-MAY-2024
DEFINITION  Mus musculus phosphorylase kinase beta (Phkb), transcript variant
            8, mRNA.
ACCESSION   NM_001403921 XM_006530531
VERSION     NM_001403921.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   Arends,C.J., Wilson,L.H., Estrella,A., Kwon,O.S., Weinstein,D.A.
            and Lee,Y.M.
  TITLE     A Mouse Model of Glycogen Storage Disease Type IX-Beta: A Role for
            Phkb in Glycogenolysis
  JOURNAL   Int J Mol Sci 23 (17), 9944 (2022)
   PUBMED   36077341
  REMARK    GeneRIF: A Mouse Model of Glycogen Storage Disease Type IX-Beta: A
            Role for Phkb in Glycogenolysis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8134)
  AUTHORS   Yang,Y., Liu,S., Lei,Z., Chen,G., Huang,L., Yang,F., Lei,Y.,
            Liu,Y., Yang,L., Liu,W., Lai,L. and Guo,J.
  TITLE     Circular RNA profile in liver tissue of EpCAM knockout mice
  JOURNAL   Int J Mol Med 44 (3), 1063-1077 (2019)
   PUBMED   31524221
REFERENCE   4  (bases 1 to 8134)
  AUTHORS   Zhang,Z., Li,Q., Diao,M., Liu,N., Cheng,W., Xiao,P., Zou,J., Su,L.,
            Yu,K., Wu,J., Li,L. and Jiang,Q.
  TITLE     Sporadic Hirschsprung Disease: Mutational Spectrum and Novel
            Candidate Genes Revealed by Next-generation Sequencing
  JOURNAL   Sci Rep 7 (1), 14796 (2017)
   PUBMED   29093530
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8134)
  AUTHORS   Arun,G., Akhade,V.S., Donakonda,S. and Rao,M.R.
  TITLE     mrhl RNA, a long noncoding RNA, negatively regulates Wnt signaling
            through its protein partner Ddx5/p68 in mouse spermatogonial cells
  JOURNAL   Mol Cell Biol 32 (15), 3140-3152 (2012)
   PUBMED   22665494
REFERENCE   6  (bases 1 to 8134)
  AUTHORS   Boulatnikov,I.G., Nadeau,O.W., Daniels,P.J., Sage,J.M.,
            Jeyasingham,M.D., Villar,M.T., Artigues,A. and Carlson,G.M.
  TITLE     The regulatory beta subunit of phosphorylase kinase interacts with
            glyceraldehyde-3-phosphate dehydrogenase
  JOURNAL   Biochemistry 47 (27), 7228-7236 (2008)
   PUBMED   18549242
  REMARK    GeneRIF: The interactions described herein between the beta subunit
            of PhK and GAPDH provide a possible mechanism for the direct
            linkage of glycogenolysis and glycolysis in skeletal muscle.
REFERENCE   7  (bases 1 to 8134)
  AUTHORS   Doyle,J., Ren,X., Lennon,G. and Stubbs,L.
  TITLE     Mutations in the Cacnl1a4 calcium channel gene are associated with
            seizures, cerebellar degeneration, and ataxia in tottering and
            leaner mutant mice
  JOURNAL   Mamm Genome 8 (2), 113-120 (1997)
   PUBMED   9060410
REFERENCE   8  (bases 1 to 8134)
  AUTHORS   Harmann,B., Zander,N.F. and Kilimann,M.W.
  TITLE     Isoform diversity of phosphorylase kinase alpha and beta subunits
            generated by alternative RNA splicing
  JOURNAL   J Biol Chem 266 (24), 15631-15637 (1991)
   PUBMED   1874721
REFERENCE   9  (bases 1 to 8134)
  AUTHORS   Chamberlain,J.S., VanTuinen,P., Reeves,A.A., Philip,B.A. and
            Caskey,C.T.
  TITLE     Isolation of cDNA clones for the catalytic gamma subunit of mouse
            muscle phosphorylase kinase: expression of mRNA in normal and
            mutant Phk mice
  JOURNAL   Proc Natl Acad Sci U S A 84 (9), 2886-2890 (1987)
   PUBMED   3472241
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117185.2, AC122830.4 and AC116323.6.
            
            On Apr 5, 2022 this sequence version replaced XM_006530531.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.10709.1,
                                           SRR10223971.8679.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-116               AC117185.2         83095-83210
            117-206             AC117185.2         117741-117830
            207-345             AC117185.2         120217-120355
            346-445             AC117185.2         121818-121917
            446-553             AC117185.2         138537-138644
            554-634             AC117185.2         143984-144064
            635-750             AC117185.2         164234-164349
            751-814             AC117185.2         177515-177578
            815-910             AC117185.2         182948-183043
            911-1108            AC117185.2         184299-184496
            1109-1166           AC117185.2         188159-188216
            1167-1244           AC122830.4         148837-148914
            1245-1403           AC122830.4         159198-159356
            1404-1498           AC122830.4         172605-172699
            1499-1554           AC122830.4         212719-212774
            1555-1648           AC122830.4         213272-213365
            1649-1732           AC122830.4         218561-218644
            1733-1837           AC122830.4         219094-219198
            1838-1920           AC122830.4         220171-220253
            1921-2011           AC122830.4         220738-220828
            2012-2073           AC122830.4         223087-223148
            2074-2236           AC122830.4         223245-223407
            2237-2318           AC122830.4         224440-224521
            2319-2376           AC122830.4         225983-226040
            2377-2467           AC122830.4         226900-226990
            2468-2670           AC122830.4         231184-231386
            2671-2805           AC122830.4         245412-245546
            2806-2935           AC122830.4         251392-251521
            2936-3043           AC116323.6         79408-79515         c
            3044-3184           AC116323.6         77278-77418         c
            3185-8134           AC116323.6         71709-76658         c
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 41.61 cM"
     gene            1..8134
                     /gene="Phkb"
                     /note="phosphorylase kinase beta"
                     /db_xref="GeneID:102093"
                     /db_xref="MGI:MGI:97578"
     exon            1..116
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..3322
                     /gene="Phkb"
                     /EC_number="2.7.11.19"
                     /note="isoform 3 is encoded by transcript variant 8;
                     phosphorylase b kinase regulatory subunit beta"
                     /codon_start=1
                     /product="phosphorylase b kinase regulatory subunit beta
                     isoform 3"
                     /protein_id="NP_001390850.1"
                     /db_xref="GeneID:102093"
                     /db_xref="MGI:MGI:97578"
                     /translation="MAGAEGLMAEVSWKLLERRARAKRSGSVYEPLKSINLPRPDNET
                     LWDKLDHYYRIVKSTMLMYQSPTTGLFPTKTCGGEEKSKVHESLYCAAGAWALALAYR
                     RIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSVFSVHTGDE
                     LLSYEEYGHLQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDF
                     GVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNR
                     QTLCSLLPRESRSHNTDAALLPCISYPAFALDDEALFSQTLDKVIRKLKGKYGFKRFL
                     RDGYRTPLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNLEQVKEYQDLL
                     TPLLHQTTEGYPVVPKYYYVPADFVECEKRNPGSQKRFPSNCGRDGKLFLWGQALYII
                     AKLLADELISPKDIDPVQRFVPLQNQRNVSMRYSNQGPLENDLVVHVALVAESQRLQV
                     FLNTYGIQTQTPQQVEPIQIWPQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIY
                     RILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIRED
                     NIRGSRFNPILDMLAAFKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEKLPEF
                     KSFEELEFPKHSKVKRQSSTADAPEAQHEPGITITEWKNKSTHEILQKLNDCGCLAGQ
                     TILLGILLKREGPNFITMEGTVSDHIERVYRRAGSKKLWLAVRYGAAFTQKFSSSIAP
                     HITTFLVHGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIH
                     IGWIISNSPELFSGMLKIRIGWIIHAMEYELQVRGGDKPAVDLYQLSPSEVKQLLLDI
                     LQPQQSGRCWLNRRQIDGSLNRTPPEFYDRVWQILERTPNGIVVAGKHLPQQPTLSDM
                     TMYEMNFSLLVEDMLGNIDQPKYRQIIVELLMVVSIVLERNPELEFQDKVDLDRLVKE
                     AFHEFQKDESRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNSLLEGEVKPSNED
                     SCLVS"
     exon            117..206
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            207..345
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            346..445
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            446..553
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            554..634
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            635..750
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            751..814
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            815..910
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1108
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1166
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1244
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1403
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1498
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1554
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1648
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1732
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1837
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..1920
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..2011
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2012..2073
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2236
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..2318
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2376
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2467
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2468..2670
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2671..2805
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..2935
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2936..3043
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            3044..3184
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            3185..8134
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3920..3925
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phkb"
                     /note="hexamer: AATATA"
     polyA_site      3937
                     /gene="Phkb"
                     /note="major polyA site"
     regulatory      4498..4503
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phkb"
                     /note="hexamer: AATAAA"
     polyA_site      4519
                     /gene="Phkb"
                     /note="major polyA site"
     regulatory      8109..8114
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phkb"
                     /note="hexamer: AATAAA"
     polyA_site      8134
                     /gene="Phkb"
ORIGIN      
        1 ggcctcgctg actggcagct ggggtggtgg gcactgtgcg atggcggggg ctgagggact
       61 catggctgaa gtaagctgga agctcttgga acgaagagcc cgggccaagc gctcaggctc
      121 agtttatgaa cctcttaaaa gcattaatct tccaagacct gataatgaaa ctctatggga
      181 taagttggat cattattaca gaattgtcaa gtcaacgatg ctgatgtatc aaagtccaac
      241 taccggtctc tttcctacta aaacatgtgg tggagaagag aagtccaaag tgcacgagag
      301 tctgtactgt gctgcgggtg cctgggcctt ggcgctagca tacaggcgca ttgatgatga
      361 caagggacgg acccatgagc tggagcactc ggccataaag tgtatgagag gaattctcta
      421 ctgctacatg cggcaggctg ataaggtcca acagtttaaa caagatcccc ggcccacaac
      481 atgtcttcac tctgttttca gtgtgcacac tggggatgag ttgctcagct atgaagaata
      541 tggccatctt cagataaatg cagtctcact ctttctcctt tacctggtag aaatgatttc
      601 ctcaggcctc cagataatct ataacacgga tgaggtttct tttattcaaa accttgtatt
      661 ttgtgtagag agagtttacc gtgttcctga ctttggtgtc tgggaaagag gaagcaaata
      721 taacaatggt agtaccgaac tgcactcaag ctctgttggt ttagcaaaag cagccttaga
      781 agcaattaat ggatttaacc tctttggaaa ccaggggtgt tcctggtcag ttatatttgt
      841 ggatctcgat gctcacaacc gcaacaggca gacattgtgc tcactgttac ccagagaatc
      901 acgctctcat aacacagatg ctgccctgct cccgtgcatc agttaccctg cgtttgcctt
      961 ggatgatgaa gcactgttca gccagacact tgataaagtg ataagaaaat tgaaaggaaa
     1021 atatggattt aagcgtttct tgcgagatgg gtacagaacg ccattggaag atcccaacag
     1081 acgctattac aaaccagcag agattaagct gtttgatggc attgaatgtg aatttcccat
     1141 attcttcctt tacatgatga tcgatggtgt ttttagaggc aatcttgaac aagtcaagga
     1201 atatcaggac cttttgacac ctttgcttca tcagaccaca gaaggatatc ctgttgtgcc
     1261 aaagtactat tatgtgccag ctgactttgt agaatgtgaa aaaagaaacc ctggtagtca
     1321 gaagcggttt ccaagtaact gtggccgtga tgggaagttg tttctttggg gacaagctct
     1381 ttatatcatt gcaaaactcc tagctgatga gttaatcagc cctaaagaca ttgaccctgt
     1441 ccagcgcttt gtcccacttc agaatcaacg taacgtgagc atgagatact ccaatcaggg
     1501 cccactggaa aatgacttgg tggttcatgt agcacttgta gcagaaagcc aacgccttca
     1561 agtttttctc aatacatatg gtattcaaac ccaaactcct cagcaggtgg aacccattca
     1621 gatctggcct cagcaggagc ttgtaaaagc ttatttccac ctgggaatca atgaaaagtt
     1681 gggtctgtct ggaaggccag ataggcccat tggctgcctt ggtacatcaa agatttatcg
     1741 cattctagga aagactgtgg tttgttaccc tatcatcttt gacctgagtg atttctacat
     1801 gtctcaggac gttctgctgc tcatagatga cataaagaac gcactacagt tcattaagca
     1861 atattggaaa atgcatggac gcccactttt tcttgttctc atccgagaag acaacataag
     1921 aggtagcaga ttcaacccaa tattagacat gctggcagcc tttaaaaagg gaataattgg
     1981 aggagtcaaa gttcatgttg atcgtttaca gacattgata tccggagctg tagtagagca
     2041 gcttgacttt ctacgaatta gtgacacaga aaagcttcca gaatttaaga gctttgaaga
     2101 actagaattt cctaagcatt caaaagtcaa acggcagagc agtaccgcag atgctcctga
     2161 ggcgcaacac gagccaggca tcaccattac cgagtggaaa aacaaatcca cccacgaaat
     2221 ccttcagaag ctgaatgact gcggttgtct ggctggtcaa accatcctgc tgggtatact
     2281 gctcaaaaga gagggcccta actttatcac aatggaagga actgtgtctg atcacattga
     2341 gagagtgtat agaagagctg gcagcaaaaa gctttggctg gctgtgcggt acggggctgc
     2401 atttacccag aaattttcct cctctatagc cccgcacatt accacctttc tggtacatgg
     2461 gaaacaggta actctaggtg cctttggaca tgaagaggag gtcatctcta acccactgtc
     2521 tccaagagtg attaagaaca taatctatta taagtgcaat acccacgatg agagggaggc
     2581 agtcattcag caagaactgg tcatccacat cggctggatc atctccaaca gccctgagtt
     2641 gttcagcggc atgctgaaaa tacgcattgg gtggatcatc cacgccatgg agtatgaact
     2701 gcaggtccgg ggcggggata agccagctgt ggacttgtac cagctctcgc ccagtgaagt
     2761 gaagcagctg ctgctagaca ttctccagcc tcaacagagt ggaagatgtt ggttgaatag
     2821 acgtcagatt gatgggtcat tgaatagaac acccccggaa ttctatgacc gagtgtggca
     2881 gatcctggaa cgcacaccta atggtatcgt tgtagctgga aagcatttgc cacagcaacc
     2941 aactttgtcg gatatgacca tgtatgagat gaatttttct ctccttgttg aagatatgct
     3001 gggaaacatt gaccagccaa agtatagaca gatcatagtt gagttactaa tggtcgtatc
     3061 cattgtgctg gaaagaaacc ctgaactgga gtttcaagac aaagtggatc tggacagact
     3121 ggttaaagaa gcgtttcatg agtttcaaaa agatgagagt cggctaaaag aaatcgaaaa
     3181 acaagatgat atgacttcct tttacaacac gcccccactg ggaaaaagag gaacctgcag
     3241 ctacttgacg aaggttgtga tgaactcact gctcgaggga gaagtcaagc ccagcaatga
     3301 ggactcctgc ctggttagct agtgcggctg gtcctgtagg gagcataggt gatggttttc
     3361 cagctgtgcc ttttgtgttg acattaacca gacagccatt agtgagtgaa cagatgtgtt
     3421 agtgtggtgg gtgctgtatt ctgtttgtca tgattggacc tttgcatgca ggtggcattc
     3481 gaatggtaag caatgattga aatcaagcgg aacccgtttc catgatttgt accagcaact
     3541 ctaatcttca ctgaaccata gaaatagttt atgaatcgaa aattcacaac tgcatgtttt
     3601 gtgatcaaat attccatcaa aattctgagt cttcgcttct ttagacttca ttttgacctt
     3661 cctgtattag aacaagcctg gcaagggctg atgctgtcct gggactcaca aggtactgga
     3721 ggatggtgat ggctatgcct ctgttgtacc acaaaaccga gacgtgtaac acacatcaga
     3781 aaaacaaaat tagcccctgg tatttaactt aagaagcttc attgttaata ataccatgtt
     3841 gtacagaact gttagatggt ttttaccttg taaacatgac tgtagttttg tattgagctg
     3901 catataaggg ttggactaaa atataatata tcaagcattt tctgagcatc tgttaaatat
     3961 aggaatacag aagtgccagt cagacagagc tgggaatcat gatacaatcc agccttaaaa
     4021 attagacaaa gataagaagt agattacttg tcttagtgtg ttaagagaag ttatgataag
     4081 atggtccgga tgaagccatc tgtaaaacct aagcctgaaa aaacagtcat gaagcccatg
     4141 cagaaagagt aacttctggt ggttgatggg cagggaagag aaattaaaga acgagaggga
     4201 ggaaaagccc ttgaaatatt taaaacttgt taaaattcag aagaaagtaa acacttgtat
     4261 ccttaagaat taaatttttt gattagctga gcaaagttgc aagggaaacg ctgtcttagt
     4321 taaaatagac atcacttgca agtctgtttc tgaatgagca gttagcttcc tattacatat
     4381 gcaaagtttg gagctttctt ctgctgcttg aaagcattcg aaatcagcaa ctgtgcgtca
     4441 gctttttaaa ttgtgaaatg caaaatttat tgttctgcaa gatattttgg gatgatcaat
     4501 aaatcttgat gttcctccat cagaagcaat gtctgctttc caattagaga aacaaaataa
     4561 tcctaagaat cagaatacag aacccatgaa atttagaagt cccatttgat ctacagagtc
     4621 cacatgtttt accagcggca ctagaggctg actggatata ttagcacatg taccgaggca
     4681 agacaagcct taaacctgat ctaaagcctt ctctgtcaca ctggcaggtt ctctgtgcta
     4741 agtttatagt acaatcaacg tctgttggaa ctcctgactg aatatcaaat tcaactgctt
     4801 ttccagagca ctgttagaag catgatgttt tccaggcttc ctttcatttt atttatgact
     4861 ttaaaaaaat catatttcag cctttaaaaa tcaaaatgtg agatcatctt gctgtcatct
     4921 tcgatgagct tgcaatgtct tatgggtgcc aacaaatatt tgctgaataa ctgaatacat
     4981 tcaacttacc accccaagta ttaaatagat accacggggt gggcaccgcc ccggcagcag
     5041 agacccatcc caactaaact aaagagtctg gaagcagcat agtggtatac agccggatag
     5101 cgagagccct gtgtcagaaa ctaagcgtgt gaatgagaag caagggaggc tggactccta
     5161 aagcagcaga aaccttccca gaaggggcca ggcaccacat aaacatagag aagcagcaaa
     5221 cggcctgcag gagtttgtct atgaccctca tattctggcc tcacggaagc tgaacagaaa
     5281 tgaggacagg tccatgggta aaagaagaaa aagcctgatg caattagaga gtggggtgag
     5341 aatggtgtga gctcactcca ggaaggaaga atggaggatg ttgttgtaat agtgagagct
     5401 cttctcgcag cagctgtgtt gtagcatcca cttactcccc agcagcagcc cctgggttga
     5461 aaaagacctc ttctagtctc accgaactgt atggacccca aatgcacgat cctttgaaca
     5521 ggtacaacta agtcatacta ggacaatagc tcagaataaa ctgggttttt gccccagtga
     5581 ctaccaccat tttgctgccc agggatcagg aaactattga gagtgggcat tttggcattt
     5641 tatctgagga ggcagatgag caagagtgac cgtgaaccct ccagagttgt ggcatctgac
     5701 agcacacaga ctgagaaatg gcacctctca ccgtgtctgt tacctgcagg ataagatgtg
     5761 gatccttagg agcggatgtg gttggtgatc acactccttg cccttctttc tctttcctat
     5821 aaggttcttt gaaattagtt aggtctgcat ttgttttgct tcgtcaagga catagatctt
     5881 gttgccatga gcagggcaca cagtttaaaa tattctctga caaactaaac cgtggctttt
     5941 tagtagtaat acgtagtcac ccttacagat gagtatccat cccttcactt ctgttttccc
     6001 agaagttatc tctcttctcc cagctagtag aggaaactgc gccctgctcc tcggtgtcag
     6061 tgtggacttg ctggtaggct ttgcatgagc ctcccccaac tcagttgtgt ggcgttgttt
     6121 tacacagtgc tgggaaccaa acctagggat ctgtgcatgc ggggaaagca ctgtacccct
     6181 gagctacctt tcctttctcc tcccagtgct gtttctattc tttttaaaat aggtatcgat
     6241 tcagtaaaag gaaagagctc accgttgaga aacatagaga aactgcaaga ggaatcctag
     6301 agagctgaaa tgccagtgtt ccagttttat tggaagcggt tcctcctttg gttattattt
     6361 ggttgttatt ctttgtgtga atgagaagcc tcccaatggg acacactgtg gaacaactgt
     6421 aggctcgata tttgtctcta tttggatggc agcttgaaag gggctccaca ctcccttcag
     6481 aatctccaca tccactaaaa taagactacc atgtcaagca ttaatagtcc tcatttgtcc
     6541 agttacaaag tccaacaaga tccataagtt attataagca aactgtttag aaaagcagtt
     6601 ttttagacaa caacaaacct ttttaaaggc tagtttgtag aaatgacatg atcgaatcag
     6661 aacccttcag gaactctcca ctgacgattt tatttaagaa tcatgcagaa gggatgtatc
     6721 ttatgaaaaa ccacgaaaag aactgcaatg aaagatgctg agcccagctt gctttgtagt
     6781 ctttggttgg cttggtttcc agctgctctc ctccatctct agagatggct atgtttaatt
     6841 tcctgacact aacccagttc tagtttcagg aaagccacct tgtgtgtgtt ttatcttaca
     6901 tatctgtgtg ttaggacgtt ggtatttttg tctttttctt ccttggcaga gcctcgctgt
     6961 cagaggttca cctgaggtgg tactggtcgt gggtgctgta gagttaacag gtcagcgact
     7021 ctccgccaac tgcgagtgat tgtcctgagc atattccaac aaagtgtgct cccagcagtt
     7081 ccaggactcc ctcctccttc ctctgccagg gctcattaac gatggttcag ggtgtgcatc
     7141 cacacagatg ggggtgagtg gggatgagtt tctgcctaga aaatctctgt ggagatgttt
     7201 tgtggtcacc gttttcacct atcccatata aaactgaact tacttccttt tcatctaccc
     7261 catcaataga atgtgaaatg tcttcctgat ccttattggg acccactcaa tgaacatcac
     7321 acacatcccc catcacgcca gtgaagagaa gcagcagcct ggacctccct tccatgcctt
     7381 gagaaacagg agtagataag agtgaatcca tgcctggcct tcagcagcac caaaccacta
     7441 ctatggaccc tttcttcctg agggctacat tctcctcagt tgtcctatgg ccaaggtggc
     7501 aggactaggc tggtagccat caggaaagaa gccaggttca gacaggatga ggttcagagg
     7561 gtcacggctc catttgaatt gctgaaaccc tgcataagct aattcagcct gctgagcatc
     7621 taccagactt ggttctgtac agagactgaa agaaaagaca gatcagtgaa aagacaacat
     7681 aaatcccatt tgtctctatt taagggtcaa gcttgatttc aaaatggtgg ccataggcaa
     7741 cagctccagg aacaaaccct aagtcctagg aattaggcca taagacacca acttcaggaa
     7801 gaaaccataa aatccagaaa caaggtcgta aaaacccctc ccaaagaaca gcctggggat
     7861 aaccacaaaa atgggaaaat atcttatcgc aggatgggcc agctgtgctg ggcagcccta
     7921 tctcctccta tggttaaaca tccatgacat agggttgtag accactggcc acctgtgatt
     7981 ctctaacacc caccaattaa attttagatt ccctaatcct tctagagaat tccctgggtt
     8041 tcctgtaaga aggcctgtgt gcctttgtca aaaaatagtt tacccctaca tgctgattat
     8101 ttttgcagaa taaatgttct ctgtttttgc atgc
//
