LOCUS       NM_001403919            8284 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus phosphorylase kinase beta (Phkb), transcript variant
            6, mRNA.
ACCESSION   NM_001403919
VERSION     NM_001403919.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8284)
  AUTHORS   Arends,C.J., Wilson,L.H., Estrella,A., Kwon,O.S., Weinstein,D.A.
            and Lee,Y.M.
  TITLE     A Mouse Model of Glycogen Storage Disease Type IX-Beta: A Role for
            Phkb in Glycogenolysis
  JOURNAL   Int J Mol Sci 23 (17), 9944 (2022)
   PUBMED   36077341
  REMARK    GeneRIF: A Mouse Model of Glycogen Storage Disease Type IX-Beta: A
            Role for Phkb in Glycogenolysis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8284)
  AUTHORS   Yang,Y., Liu,S., Lei,Z., Chen,G., Huang,L., Yang,F., Lei,Y.,
            Liu,Y., Yang,L., Liu,W., Lai,L. and Guo,J.
  TITLE     Circular RNA profile in liver tissue of EpCAM knockout mice
  JOURNAL   Int J Mol Med 44 (3), 1063-1077 (2019)
   PUBMED   31524221
REFERENCE   4  (bases 1 to 8284)
  AUTHORS   Zhang,Z., Li,Q., Diao,M., Liu,N., Cheng,W., Xiao,P., Zou,J., Su,L.,
            Yu,K., Wu,J., Li,L. and Jiang,Q.
  TITLE     Sporadic Hirschsprung Disease: Mutational Spectrum and Novel
            Candidate Genes Revealed by Next-generation Sequencing
  JOURNAL   Sci Rep 7 (1), 14796 (2017)
   PUBMED   29093530
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8284)
  AUTHORS   Arun,G., Akhade,V.S., Donakonda,S. and Rao,M.R.
  TITLE     mrhl RNA, a long noncoding RNA, negatively regulates Wnt signaling
            through its protein partner Ddx5/p68 in mouse spermatogonial cells
  JOURNAL   Mol Cell Biol 32 (15), 3140-3152 (2012)
   PUBMED   22665494
REFERENCE   6  (bases 1 to 8284)
  AUTHORS   Boulatnikov,I.G., Nadeau,O.W., Daniels,P.J., Sage,J.M.,
            Jeyasingham,M.D., Villar,M.T., Artigues,A. and Carlson,G.M.
  TITLE     The regulatory beta subunit of phosphorylase kinase interacts with
            glyceraldehyde-3-phosphate dehydrogenase
  JOURNAL   Biochemistry 47 (27), 7228-7236 (2008)
   PUBMED   18549242
  REMARK    GeneRIF: The interactions described herein between the beta subunit
            of PhK and GAPDH provide a possible mechanism for the direct
            linkage of glycogenolysis and glycolysis in skeletal muscle.
REFERENCE   7  (bases 1 to 8284)
  AUTHORS   Doyle,J., Ren,X., Lennon,G. and Stubbs,L.
  TITLE     Mutations in the Cacnl1a4 calcium channel gene are associated with
            seizures, cerebellar degeneration, and ataxia in tottering and
            leaner mutant mice
  JOURNAL   Mamm Genome 8 (2), 113-120 (1997)
   PUBMED   9060410
REFERENCE   8  (bases 1 to 8284)
  AUTHORS   Harmann,B., Zander,N.F. and Kilimann,M.W.
  TITLE     Isoform diversity of phosphorylase kinase alpha and beta subunits
            generated by alternative RNA splicing
  JOURNAL   J Biol Chem 266 (24), 15631-15637 (1991)
   PUBMED   1874721
REFERENCE   9  (bases 1 to 8284)
  AUTHORS   Chamberlain,J.S., VanTuinen,P., Reeves,A.A., Philip,B.A. and
            Caskey,C.T.
  TITLE     Isolation of cDNA clones for the catalytic gamma subunit of mouse
            muscle phosphorylase kinase: expression of mRNA in normal and
            mutant Phk mice
  JOURNAL   Proc Natl Acad Sci U S A 84 (9), 2886-2890 (1987)
   PUBMED   3472241
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117185.2, AC122830.4 and AC116323.6.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784647.818000.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849374,
                                           SAMN00849380 [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-116               AC117185.2         83095-83210
            117-208             AC117185.2         85128-85219
            209-266             AC117185.2         90781-90838
            267-356             AC117185.2         117741-117830
            357-495             AC117185.2         120217-120355
            496-595             AC117185.2         121818-121917
            596-703             AC117185.2         138537-138644
            704-784             AC117185.2         143984-144064
            785-900             AC117185.2         164234-164349
            901-964             AC117185.2         177515-177578
            965-1060            AC117185.2         182948-183043
            1061-1258           AC117185.2         184299-184496
            1259-1316           AC117185.2         188159-188216
            1317-1394           AC122830.4         148837-148914
            1395-1553           AC122830.4         159198-159356
            1554-1648           AC122830.4         172605-172699
            1649-1704           AC122830.4         212719-212774
            1705-1798           AC122830.4         213272-213365
            1799-1882           AC122830.4         218561-218644
            1883-1987           AC122830.4         219094-219198
            1988-2070           AC122830.4         220171-220253
            2071-2161           AC122830.4         220738-220828
            2162-2223           AC122830.4         223087-223148
            2224-2386           AC122830.4         223245-223407
            2387-2468           AC122830.4         224440-224521
            2469-2526           AC122830.4         225983-226040
            2527-2617           AC122830.4         228159-228249
            2618-2820           AC122830.4         231184-231386
            2821-2955           AC122830.4         245412-245546
            2956-3085           AC122830.4         251392-251521
            3086-3193           AC116323.6         79408-79515         c
            3194-3334           AC116323.6         77278-77418         c
            3335-8284           AC116323.6         71709-76658         c
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 41.61 cM"
     gene            1..8284
                     /gene="Phkb"
                     /note="phosphorylase kinase beta"
                     /db_xref="GeneID:102093"
                     /db_xref="MGI:MGI:97578"
     exon            1..116
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            117..208
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            209..266
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            267..356
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            357..495
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     CDS             368..3472
                     /gene="Phkb"
                     /EC_number="2.7.11.19"
                     /note="isoform 2 is encoded by transcript variant 6;
                     phosphorylase b kinase regulatory subunit beta"
                     /codon_start=1
                     /product="phosphorylase b kinase regulatory subunit beta
                     isoform 2"
                     /protein_id="NP_001390848.1"
                     /db_xref="GeneID:102093"
                     /db_xref="MGI:MGI:97578"
                     /translation="MLMYQSPTTGLFPTKTCGGEEKSKVHESLYCAAGAWALALAYRR
                     IDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSVFSVHTGDEL
                     LSYEEYGHLQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFG
                     VWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQ
                     TLCSLLPRESRSHNTDAALLPCISYPAFALDDEALFSQTLDKVIRKLKGKYGFKRFLR
                     DGYRTPLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNLEQVKEYQDLLT
                     PLLHQTTEGYPVVPKYYYVPADFVECEKRNPGSQKRFPSNCGRDGKLFLWGQALYIIA
                     KLLADELISPKDIDPVQRFVPLQNQRNVSMRYSNQGPLENDLVVHVALVAESQRLQVF
                     LNTYGIQTQTPQQVEPIQIWPQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYR
                     ILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDN
                     IRGSRFNPILDMLAAFKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEKLPEFK
                     SFEELEFPKHSKVKRQSSTADAPEAQHEPGITITEWKNKSTHEILQKLNDCGCLAGQT
                     ILLGILLKREGPNFITMEGTVSDHIERVYRRAGSKKLWSVVRRAASLLNKVVDSLAPS
                     ITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIHI
                     GWIISNSPELFSGMLKIRIGWIIHAMEYELQVRGGDKPAVDLYQLSPSEVKQLLLDIL
                     QPQQSGRCWLNRRQIDGSLNRTPPEFYDRVWQILERTPNGIVVAGKHLPQQPTLSDMT
                     MYEMNFSLLVEDMLGNIDQPKYRQIIVELLMVVSIVLERNPELEFQDKVDLDRLVKEA
                     FHEFQKDESRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNSLLEGEVKPSNEDS
                     CLVS"
     exon            496..595
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            596..703
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            704..784
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            785..900
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            901..964
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1060
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1258
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1316
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1394
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1553
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1648
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1704
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1798
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1882
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..1987
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            1988..2070
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2161
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2223
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..2386
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2387..2468
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2469..2526
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2527..2617
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2618..2820
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2821..2955
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            2956..3085
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            3086..3193
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            3194..3334
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     exon            3335..8284
                     /gene="Phkb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4070..4075
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phkb"
                     /note="hexamer: AATATA"
     polyA_site      4087
                     /gene="Phkb"
                     /note="major polyA site"
     regulatory      4648..4653
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phkb"
                     /note="hexamer: AATAAA"
     polyA_site      4669
                     /gene="Phkb"
                     /note="major polyA site"
     regulatory      8259..8264
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phkb"
                     /note="hexamer: AATAAA"
     polyA_site      8284
                     /gene="Phkb"
ORIGIN      
        1 ggcctcgctg actggcagct ggggtggtgg gcactgtgcg atggcggggg ctgagggact
       61 catggctgaa gtaagctgga agctcttgga acgaagagcc cgggccaagc gctcagttta
      121 gcgtgcaacg cttatgataa gaatcaacag tttgaaatgg ccaactcacc tgatgcagcc
      181 ttctcttcac ctgctttgtt aaggtctggt ctcaagtcat gtgtcccaaa gtttctgatc
      241 aacgtatgtt ctccttttct ctcctggctc agtttatgaa cctcttaaaa gcattaatct
      301 tccaagacct gataatgaaa ctctatggga taagttggat cattattaca gaattgtcaa
      361 gtcaacgatg ctgatgtatc aaagtccaac taccggtctc tttcctacta aaacatgtgg
      421 tggagaagag aagtccaaag tgcacgagag tctgtactgt gctgcgggtg cctgggcctt
      481 ggcgctagca tacaggcgca ttgatgatga caagggacgg acccatgagc tggagcactc
      541 ggccataaag tgtatgagag gaattctcta ctgctacatg cggcaggctg ataaggtcca
      601 acagtttaaa caagatcccc ggcccacaac atgtcttcac tctgttttca gtgtgcacac
      661 tggggatgag ttgctcagct atgaagaata tggccatctt cagataaatg cagtctcact
      721 ctttctcctt tacctggtag aaatgatttc ctcaggcctc cagataatct ataacacgga
      781 tgaggtttct tttattcaaa accttgtatt ttgtgtagag agagtttacc gtgttcctga
      841 ctttggtgtc tgggaaagag gaagcaaata taacaatggt agtaccgaac tgcactcaag
      901 ctctgttggt ttagcaaaag cagccttaga agcaattaat ggatttaacc tctttggaaa
      961 ccaggggtgt tcctggtcag ttatatttgt ggatctcgat gctcacaacc gcaacaggca
     1021 gacattgtgc tcactgttac ccagagaatc acgctctcat aacacagatg ctgccctgct
     1081 cccgtgcatc agttaccctg cgtttgcctt ggatgatgaa gcactgttca gccagacact
     1141 tgataaagtg ataagaaaat tgaaaggaaa atatggattt aagcgtttct tgcgagatgg
     1201 gtacagaacg ccattggaag atcccaacag acgctattac aaaccagcag agattaagct
     1261 gtttgatggc attgaatgtg aatttcccat attcttcctt tacatgatga tcgatggtgt
     1321 ttttagaggc aatcttgaac aagtcaagga atatcaggac cttttgacac ctttgcttca
     1381 tcagaccaca gaaggatatc ctgttgtgcc aaagtactat tatgtgccag ctgactttgt
     1441 agaatgtgaa aaaagaaacc ctggtagtca gaagcggttt ccaagtaact gtggccgtga
     1501 tgggaagttg tttctttggg gacaagctct ttatatcatt gcaaaactcc tagctgatga
     1561 gttaatcagc cctaaagaca ttgaccctgt ccagcgcttt gtcccacttc agaatcaacg
     1621 taacgtgagc atgagatact ccaatcaggg cccactggaa aatgacttgg tggttcatgt
     1681 agcacttgta gcagaaagcc aacgccttca agtttttctc aatacatatg gtattcaaac
     1741 ccaaactcct cagcaggtgg aacccattca gatctggcct cagcaggagc ttgtaaaagc
     1801 ttatttccac ctgggaatca atgaaaagtt gggtctgtct ggaaggccag ataggcccat
     1861 tggctgcctt ggtacatcaa agatttatcg cattctagga aagactgtgg tttgttaccc
     1921 tatcatcttt gacctgagtg atttctacat gtctcaggac gttctgctgc tcatagatga
     1981 cataaagaac gcactacagt tcattaagca atattggaaa atgcatggac gcccactttt
     2041 tcttgttctc atccgagaag acaacataag aggtagcaga ttcaacccaa tattagacat
     2101 gctggcagcc tttaaaaagg gaataattgg aggagtcaaa gttcatgttg atcgtttaca
     2161 gacattgata tccggagctg tagtagagca gcttgacttt ctacgaatta gtgacacaga
     2221 aaagcttcca gaatttaaga gctttgaaga actagaattt cctaagcatt caaaagtcaa
     2281 acggcagagc agtaccgcag atgctcctga ggcgcaacac gagccaggca tcaccattac
     2341 cgagtggaaa aacaaatcca cccacgaaat ccttcagaag ctgaatgact gcggttgtct
     2401 ggctggtcaa accatcctgc tgggtatact gctcaaaaga gagggcccta actttatcac
     2461 aatggaagga actgtgtctg atcacattga gagagtgtat agaagagctg gcagcaaaaa
     2521 gctttggtcg gttgtacgcc gtgcagcaag tcttttaaat aaagtagtgg acagcctggc
     2581 cccatccatt actaatgttt tagtgcaggg caaacaggta actctaggtg cctttggaca
     2641 tgaagaggag gtcatctcta acccactgtc tccaagagtg attaagaaca taatctatta
     2701 taagtgcaat acccacgatg agagggaggc agtcattcag caagaactgg tcatccacat
     2761 cggctggatc atctccaaca gccctgagtt gttcagcggc atgctgaaaa tacgcattgg
     2821 gtggatcatc cacgccatgg agtatgaact gcaggtccgg ggcggggata agccagctgt
     2881 ggacttgtac cagctctcgc ccagtgaagt gaagcagctg ctgctagaca ttctccagcc
     2941 tcaacagagt ggaagatgtt ggttgaatag acgtcagatt gatgggtcat tgaatagaac
     3001 acccccggaa ttctatgacc gagtgtggca gatcctggaa cgcacaccta atggtatcgt
     3061 tgtagctgga aagcatttgc cacagcaacc aactttgtcg gatatgacca tgtatgagat
     3121 gaatttttct ctccttgttg aagatatgct gggaaacatt gaccagccaa agtatagaca
     3181 gatcatagtt gagttactaa tggtcgtatc cattgtgctg gaaagaaacc ctgaactgga
     3241 gtttcaagac aaagtggatc tggacagact ggttaaagaa gcgtttcatg agtttcaaaa
     3301 agatgagagt cggctaaaag aaatcgaaaa acaagatgat atgacttcct tttacaacac
     3361 gcccccactg ggaaaaagag gaacctgcag ctacttgacg aaggttgtga tgaactcact
     3421 gctcgaggga gaagtcaagc ccagcaatga ggactcctgc ctggttagct agtgcggctg
     3481 gtcctgtagg gagcataggt gatggttttc cagctgtgcc ttttgtgttg acattaacca
     3541 gacagccatt agtgagtgaa cagatgtgtt agtgtggtgg gtgctgtatt ctgtttgtca
     3601 tgattggacc tttgcatgca ggtggcattc gaatggtaag caatgattga aatcaagcgg
     3661 aacccgtttc catgatttgt accagcaact ctaatcttca ctgaaccata gaaatagttt
     3721 atgaatcgaa aattcacaac tgcatgtttt gtgatcaaat attccatcaa aattctgagt
     3781 cttcgcttct ttagacttca ttttgacctt cctgtattag aacaagcctg gcaagggctg
     3841 atgctgtcct gggactcaca aggtactgga ggatggtgat ggctatgcct ctgttgtacc
     3901 acaaaaccga gacgtgtaac acacatcaga aaaacaaaat tagcccctgg tatttaactt
     3961 aagaagcttc attgttaata ataccatgtt gtacagaact gttagatggt ttttaccttg
     4021 taaacatgac tgtagttttg tattgagctg catataaggg ttggactaaa atataatata
     4081 tcaagcattt tctgagcatc tgttaaatat aggaatacag aagtgccagt cagacagagc
     4141 tgggaatcat gatacaatcc agccttaaaa attagacaaa gataagaagt agattacttg
     4201 tcttagtgtg ttaagagaag ttatgataag atggtccgga tgaagccatc tgtaaaacct
     4261 aagcctgaaa aaacagtcat gaagcccatg cagaaagagt aacttctggt ggttgatggg
     4321 cagggaagag aaattaaaga acgagaggga ggaaaagccc ttgaaatatt taaaacttgt
     4381 taaaattcag aagaaagtaa acacttgtat ccttaagaat taaatttttt gattagctga
     4441 gcaaagttgc aagggaaacg ctgtcttagt taaaatagac atcacttgca agtctgtttc
     4501 tgaatgagca gttagcttcc tattacatat gcaaagtttg gagctttctt ctgctgcttg
     4561 aaagcattcg aaatcagcaa ctgtgcgtca gctttttaaa ttgtgaaatg caaaatttat
     4621 tgttctgcaa gatattttgg gatgatcaat aaatcttgat gttcctccat cagaagcaat
     4681 gtctgctttc caattagaga aacaaaataa tcctaagaat cagaatacag aacccatgaa
     4741 atttagaagt cccatttgat ctacagagtc cacatgtttt accagcggca ctagaggctg
     4801 actggatata ttagcacatg taccgaggca agacaagcct taaacctgat ctaaagcctt
     4861 ctctgtcaca ctggcaggtt ctctgtgcta agtttatagt acaatcaacg tctgttggaa
     4921 ctcctgactg aatatcaaat tcaactgctt ttccagagca ctgttagaag catgatgttt
     4981 tccaggcttc ctttcatttt atttatgact ttaaaaaaat catatttcag cctttaaaaa
     5041 tcaaaatgtg agatcatctt gctgtcatct tcgatgagct tgcaatgtct tatgggtgcc
     5101 aacaaatatt tgctgaataa ctgaatacat tcaacttacc accccaagta ttaaatagat
     5161 accacggggt gggcaccgcc ccggcagcag agacccatcc caactaaact aaagagtctg
     5221 gaagcagcat agtggtatac agccggatag cgagagccct gtgtcagaaa ctaagcgtgt
     5281 gaatgagaag caagggaggc tggactccta aagcagcaga aaccttccca gaaggggcca
     5341 ggcaccacat aaacatagag aagcagcaaa cggcctgcag gagtttgtct atgaccctca
     5401 tattctggcc tcacggaagc tgaacagaaa tgaggacagg tccatgggta aaagaagaaa
     5461 aagcctgatg caattagaga gtggggtgag aatggtgtga gctcactcca ggaaggaaga
     5521 atggaggatg ttgttgtaat agtgagagct cttctcgcag cagctgtgtt gtagcatcca
     5581 cttactcccc agcagcagcc cctgggttga aaaagacctc ttctagtctc accgaactgt
     5641 atggacccca aatgcacgat cctttgaaca ggtacaacta agtcatacta ggacaatagc
     5701 tcagaataaa ctgggttttt gccccagtga ctaccaccat tttgctgccc agggatcagg
     5761 aaactattga gagtgggcat tttggcattt tatctgagga ggcagatgag caagagtgac
     5821 cgtgaaccct ccagagttgt ggcatctgac agcacacaga ctgagaaatg gcacctctca
     5881 ccgtgtctgt tacctgcagg ataagatgtg gatccttagg agcggatgtg gttggtgatc
     5941 acactccttg cccttctttc tctttcctat aaggttcttt gaaattagtt aggtctgcat
     6001 ttgttttgct tcgtcaagga catagatctt gttgccatga gcagggcaca cagtttaaaa
     6061 tattctctga caaactaaac cgtggctttt tagtagtaat acgtagtcac ccttacagat
     6121 gagtatccat cccttcactt ctgttttccc agaagttatc tctcttctcc cagctagtag
     6181 aggaaactgc gccctgctcc tcggtgtcag tgtggacttg ctggtaggct ttgcatgagc
     6241 ctcccccaac tcagttgtgt ggcgttgttt tacacagtgc tgggaaccaa acctagggat
     6301 ctgtgcatgc ggggaaagca ctgtacccct gagctacctt tcctttctcc tcccagtgct
     6361 gtttctattc tttttaaaat aggtatcgat tcagtaaaag gaaagagctc accgttgaga
     6421 aacatagaga aactgcaaga ggaatcctag agagctgaaa tgccagtgtt ccagttttat
     6481 tggaagcggt tcctcctttg gttattattt ggttgttatt ctttgtgtga atgagaagcc
     6541 tcccaatggg acacactgtg gaacaactgt aggctcgata tttgtctcta tttggatggc
     6601 agcttgaaag gggctccaca ctcccttcag aatctccaca tccactaaaa taagactacc
     6661 atgtcaagca ttaatagtcc tcatttgtcc agttacaaag tccaacaaga tccataagtt
     6721 attataagca aactgtttag aaaagcagtt ttttagacaa caacaaacct ttttaaaggc
     6781 tagtttgtag aaatgacatg atcgaatcag aacccttcag gaactctcca ctgacgattt
     6841 tatttaagaa tcatgcagaa gggatgtatc ttatgaaaaa ccacgaaaag aactgcaatg
     6901 aaagatgctg agcccagctt gctttgtagt ctttggttgg cttggtttcc agctgctctc
     6961 ctccatctct agagatggct atgtttaatt tcctgacact aacccagttc tagtttcagg
     7021 aaagccacct tgtgtgtgtt ttatcttaca tatctgtgtg ttaggacgtt ggtatttttg
     7081 tctttttctt ccttggcaga gcctcgctgt cagaggttca cctgaggtgg tactggtcgt
     7141 gggtgctgta gagttaacag gtcagcgact ctccgccaac tgcgagtgat tgtcctgagc
     7201 atattccaac aaagtgtgct cccagcagtt ccaggactcc ctcctccttc ctctgccagg
     7261 gctcattaac gatggttcag ggtgtgcatc cacacagatg ggggtgagtg gggatgagtt
     7321 tctgcctaga aaatctctgt ggagatgttt tgtggtcacc gttttcacct atcccatata
     7381 aaactgaact tacttccttt tcatctaccc catcaataga atgtgaaatg tcttcctgat
     7441 ccttattggg acccactcaa tgaacatcac acacatcccc catcacgcca gtgaagagaa
     7501 gcagcagcct ggacctccct tccatgcctt gagaaacagg agtagataag agtgaatcca
     7561 tgcctggcct tcagcagcac caaaccacta ctatggaccc tttcttcctg agggctacat
     7621 tctcctcagt tgtcctatgg ccaaggtggc aggactaggc tggtagccat caggaaagaa
     7681 gccaggttca gacaggatga ggttcagagg gtcacggctc catttgaatt gctgaaaccc
     7741 tgcataagct aattcagcct gctgagcatc taccagactt ggttctgtac agagactgaa
     7801 agaaaagaca gatcagtgaa aagacaacat aaatcccatt tgtctctatt taagggtcaa
     7861 gcttgatttc aaaatggtgg ccataggcaa cagctccagg aacaaaccct aagtcctagg
     7921 aattaggcca taagacacca acttcaggaa gaaaccataa aatccagaaa caaggtcgta
     7981 aaaacccctc ccaaagaaca gcctggggat aaccacaaaa atgggaaaat atcttatcgc
     8041 aggatgggcc agctgtgctg ggcagcccta tctcctccta tggttaaaca tccatgacat
     8101 agggttgtag accactggcc acctgtgatt ctctaacacc caccaattaa attttagatt
     8161 ccctaatcct tctagagaat tccctgggtt tcctgtaaga aggcctgtgt gcctttgtca
     8221 aaaaatagtt tacccctaca tgctgattat ttttgcagaa taaatgttct ctgtttttgc
     8281 atgc
//
