LOCUS       NM_172783               4986 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript
            variant 1, mRNA.
ACCESSION   NM_172783
VERSION     NM_172783.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Conte,D., Garaffo,G., Lo Iacono,N., Mantero,S., Piccolo,S.,
            Cordenonsi,M., Perez-Morga,D., Orecchia,V., Poli,V. and Merlo,G.R.
  TITLE     The apical ectodermal ridge of the mouse model of ectrodactyly
            Dlx5;Dlx6-/- shows altered stratification and cell polarity, which
            are restored by exogenous Wnt5a ligand
  JOURNAL   Hum Mol Genet 25 (4), 740-754 (2016)
   PUBMED   26685160
REFERENCE   3  (bases 1 to 4986)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 4986)
  AUTHORS   Liu,S.P., Li,Y.S., Chen,Y.J., Chiang,E.P., Li,A.F., Lee,Y.H.,
            Tsai,T.F., Hsiao,M., Huang,S.F. and Chen,Y.M.
  TITLE     Glycine N-methyltransferase-/- mice develop chronic hepatitis and
            glycogen storage disease in the liver
  JOURNAL   Hepatology 46 (5), 1413-1425 (2007)
   PUBMED   17937387
  REMARK    Erratum:[Hepatology. 2008 Feb;47(2):769. Hwang, Shiu-Feng
            [corrected to Huang, Shiu-Feng]]
REFERENCE   5  (bases 1 to 4986)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   6  (bases 1 to 4986)
  AUTHORS   Amar,L.C., Dandolo,L., Hanauer,A., Cook,A.R., Arnaud,D.,
            Mandel,J.L. and Avner,P.
  TITLE     Conservation and reorganization of loci on the mammalian X
            chromosome: a molecular framework for the identification of
            homologous subchromosomal regions in man and mouse
  JOURNAL   Genomics 2 (3), 220-230 (1988)
   PUBMED   2899541
REFERENCE   7  (bases 1 to 4986)
  AUTHORS   Chamberlain,J.S., VanTuinen,P., Reeves,A.A., Philip,B.A. and
            Caskey,C.T.
  TITLE     Isolation of cDNA clones for the catalytic gamma subunit of mouse
            muscle phosphorylase kinase: expression of mRNA in normal and
            mutant Phk mice
  JOURNAL   Proc Natl Acad Sci U S A 84 (9), 2886-2890 (1987)
   PUBMED   3472241
REFERENCE   8  (bases 1 to 4986)
  AUTHORS   Varsanyi,M., Vrbica,A. and Heilmeyer,L.M. Jr.
  TITLE     X-linked dominant inheritance of partial phosphorylase kinase
            deficiency in mice
  JOURNAL   Biochem Genet 18 (3-4), 247-261 (1980)
   PUBMED   7447922
REFERENCE   9  (bases 1 to 4986)
  AUTHORS   Cohen,P.T., Burchell,A. and Cohen,P.
  TITLE     The molecular basis of skeletal muscle phosphorylase kinase
            deficiency
  JOURNAL   Eur J Biochem 66 (2), 347-356 (1976)
   PUBMED   820556
REFERENCE   10 (bases 1 to 4986)
  AUTHORS   Huijing,F., Eicher,E.M. and Coleman,D.L.
  TITLE     Location of phosphorylase kinase (Phk) in the mouse X chromosome
  JOURNAL   Biochem Genet 9 (2), 193-196 (1973)
   PUBMED   4726088
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL731801.11, BB662591.1 and AK052346.1.
            
            On Dec 15, 2017 this sequence version replaced NM_172783.3.
            
            Sequence Note: An in-frame AUG is located 5 codons upstream of the
            annotated translation start site but is not being annotated as a
            start site since it is not conserved and is in a weak Kozak
            sequence context.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK052346.1, SRR1660825.130521.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-272               AL731801.11        143087-143358
            273-528             BB662591.1         2-257
            529-4986            AK052346.1         2-4459
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 73.95 cM"
     gene            1..4986
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /note="phosphorylase kinase alpha 2"
                     /db_xref="GeneID:110094"
                     /db_xref="MGI:MGI:97577"
     exon            1..743
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     CDS             666..4373
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /EC_number="2.7.11.19"
                     /note="isoform a is encoded by transcript variant 1;
                     phosphorylase kinase alpha L subunit"
                     /codon_start=1
                     /product="phosphorylase b kinase regulatory subunit alpha,
                     liver isoform isoform a"
                     /protein_id="NP_766371.1"
                     /db_xref="CCDS:CCDS30504.1"
                     /db_xref="GeneID:110094"
                     /db_xref="MGI:MGI:97577"
                     /translation="MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWV
                     RDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKF
                     KHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLD
                     EVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLF
                     GAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLV
                     NVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTY
                     LIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDR
                     VPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSH
                     IKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTS
                     KLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPV
                     THTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCD
                     EKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCK
                     KSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLL
                     KTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQ
                     HGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLP
                     PEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLR
                     LRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRP
                     IHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNS
                     LHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKV
                     WKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMV
                     LTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSA
                     PSGAYGTMTYLTKAVASHLQELLPSSGCQMQ"
     exon            744..902
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            903..950
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1119
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1202
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1283
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1382
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1529
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1583
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1706
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1802
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1910
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..1989
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2124
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            2125..2234
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            2235..2379
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2458
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            2459..2628
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            2629..2802
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            2803..2891
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            2892..3025
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            3026..3182
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            3183..3262
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            3263..3341
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            3342..3471
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            3472..3573
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            3574..3692
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            3693..3722
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            3723..3776
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            3777..3947
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            3948..4001
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            4002..4202
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     exon            4203..4986
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4383..4388
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /note="hexamer: CATAAA"
     polyA_site      4405
                     /gene="Phka2"
                     /gene_synonym="6330505C01Rik; D330034O08; Phk"
                     /note="major polyA site"
ORIGIN      
        1 ccttccgggc gggactgcac gggttccgct tcttggttcg ggccggcgtg aggggtggcc
       61 tccgtacccc gggggccgcg accgaaggac tctgctcctt tagcctcagc cgcggttgga
      121 agcaggttga ggtaggagca aagtcaataa aaaaaactga atttccgtta agagcccagc
      181 tccccgccgc tcccagcggc cttcctctgt agaccctctc cctttaggcg gagacttgga
      241 gagtccgccc cacgaggggg gcggagccta gtaaagctta ggtgagctcc caagcaaccc
      301 ggagccgcct cccaaaacag aggggtaccg ccaaggcctt tctccagcct agtgcccagt
      361 ctaggttctg tcgagcatgg tggtgggact agaaaccctc ggggcagctg ctgaccggga
      421 ggaagccggg ccgcgatagc gccacccccg tctcctcccc cgcaccgccc agcggcgcga
      481 cactctggtt ggctcgcctt gcaagtcata ctttgggtct ctcagtttga aaactcttcc
      541 tctcggcccc ctttgaagaa actcattgac gctgaatgtt ttgaggacca ggtcagcttg
      601 gaggcggcaa ctttggcgga caccagcggg caggtccagc tccgtggccc atgagcttgg
      661 gcgagatgcg gagcaggagc aattctgggg tccgcctgga cgggtacgcg aggctggtgc
      721 agcaaaccat cctgtgttac cagaacccag tcactgggct gttatcagcc agccatgacc
      781 agaaggatgc ctgggtacgg gacaatatct atagcatatt ggctgtttgg ggcctgggca
      841 tggcctaccg caagaatgct gaccgtgatg aggataaagc caaggcctat gagttggaac
      901 agaacgtagt gaagctgatg cgaggtcttc tccagtgtat gatgaggcag gtggacaaag
      961 tggagaagtt caagcacact cagagtacca aggacagtct acatgccaag tacaacactg
     1021 ccacatgtag cactgtggtg ggtgatgacc agtggggcca cctccaggtg gatgccacct
     1081 ctctcttcct cctgttcctg gcccagatga cagcctcagg tttgcgtatt atttttaccc
     1141 ttgacgaagt ggccttcata cagaatcttg tcttctacat agaagctgca tataaagttg
     1201 ctgattatgg gatgtgggag cgtggagata aaaccaatca gggcattccg gaactgaatg
     1261 caagctcggt gggagtggcc aaggcagcac ttgaggccat tgatgaacta gatctttttg
     1321 gagcccatgg aggacgcaaa tcggtgatcc acgtcctacc tgatgaagtt gagcactgcc
     1381 agtcaattct tttctccatg ttgccaagag catcaacatc taaagaaatc gatgctggac
     1441 ttctttctat tatttctttc ccggcctttg cagtagaaga tgtgaacctt gtgaatgtga
     1501 ccaaaaatga aattatttcc aagcttcagg ggcggtatgg atgctgtcgc ttccttcgag
     1561 atggctataa aaccccaaga gaggatccac accgattgca ttatgaccct gccgaactta
     1621 agctctttga aaacattgaa tgtgaatggc ctgtgttctg gacttattta atcatagatg
     1681 gaatcttcaa tggtgatgct gttcaggtcc aagaataccg agaagccttg gagggaatat
     1741 taatcagagg caaagatggg atccacttgg tgccagaact ctatgccatc ccaccagaca
     1801 aggtggatga agagtataag aatccacaca cagtagaccg agttccactg ggaaagctgc
     1861 cccatctttg gggacagtcc ttgtacatcc tcagctcact gctagcagag ggattccttg
     1921 ccacgggtga aatcgatccc ttaaatagaa gattttctac ttcagtcaaa cctgatgttg
     1981 tagtgcaagt tgctgttttg gcagaaaaca gtcacattaa ggggctgttg aaggagcatg
     2041 gaatgactgt ccagagcatt gctgatgtgc atccgattcg agtccagcca ggccgaattc
     2101 ttagtcacat atatgccaaa cttggacgaa ataagaatat gaaattgagt ggtcgaccgt
     2161 atcggcacat tggtgtcctt ggcacctcta aactctatgt gattaggaat cacatcttca
     2221 cttttacacc ccagttcact gaccagcatc acttctacct ggccctggac aatgagatga
     2281 ttgtggagat gctgaggatc gaacttgcct atctgtgcac ctgctggcgg atgaccggcc
     2341 gacccactct caccttccct gtcacgcata ccatgctcac aaatgatgga tcagacattc
     2401 atcctgcagt tctttctaca attagaaaac tagaagatgg ctattttgga ggtgctagag
     2461 taaaactagg aaatctggca gagttcctta ctacctcatt ctacacacac ctgaccttcc
     2521 tggatccaga ctgcgatgag aagttgtttg gtgacatcac tgataggagc tttagtcctg
     2581 acagtgagcc agacctggga ggatacctgg aagacagcag tcctcaagaa agccaagatg
     2641 aacttgacca gtatatcagc caccttcttc aaagcacatc cttgaagtgt tacctgcccc
     2701 ctctttgtaa gaagtcagaa gacagccatt ttttcagtgc tattcactcc actcgggaca
     2761 tactttctgt gatggccaaa gcgaagggtt tggaaactac attttttccc atgattttgc
     2821 caactaaagt tctaagtgga caccgtaagt cactgaatct tgttgactcc cctcagccac
     2881 tcctaaagac gactcctgaa tatgactacc agtggcccag agacgaccat gatgaagtgg
     2941 actgtgagaa gctagttggg caactgaaag actgctcaaa cctacaggac caagcagaca
     3001 ttctgtacat tctttatgta atgaagggtc cccgctggga taccaatttg tttgggcagc
     3061 atggagtcac tgttcacagt cttctcagcg agctctatgg aaaagctggc ctaaaccaag
     3121 aatggagttt gattcgctac atttcaggcc tgctcaggaa gaaagtggag gtcctggctg
     3181 aggcctgtgc agatctgctg tcccaccaga agcagcttac agtaggactg ccccctgagc
     3241 cccgggagaa gaccatatct acgcctctac ccccagagga gctcacagaa ctcatatatg
     3301 aagccagtgg acaggacatc agcattgctg tcctcacaca ggagatcgtg gtttacctgg
     3361 ccatgtatgt ccgggcccag cctagcctct ttgcagagat gctcagactc cggattggat
     3421 tgatcatcca ggtgatggcc acagagctgg ctcggagcct gaactgctca ggaaaagaag
     3481 cttcggagag cttgatgaac ctcagccctt tcgacatgaa gagccttctg catcatatcc
     3541 tgagtgggaa ggagttcggt gtggagagaa gcgtgcgccc aatacactcc tccatgtcca
     3601 gccctgccat ctccatccat gaggtgggtc atactggagc caccaaaact gagcggagtg
     3661 gcatcaccag actgaggagt gagatgaaac agatgaatag gcgagctagt gctgatgagc
     3721 agttctttcc tttgggccag accatgtcca acagtttgca ttccatcaag tctgtgaggt
     3781 ccagcacccc atcctccccg acaggcacat catctacaga ctctggagga cagcacctgg
     3841 gctggggaga acagcagggc cagtggctgc gccggagaag gttggatggg gccattaaca
     3901 gggtccccgt gggattctac cagaaagtgt ggaagatcct tcagaagtgc catggcctgt
     3961 ccatcgatgg ttacgtcctc ccatcctcga caacccaaga gatgacccct tgtgagatca
     4021 agtttgccgt ccatgtggag tcagtgctca accgtgtgtc ccagcctgag tatcggcagc
     4081 tactggtaga agccatcatg gtactgactc tgctctcaga taccgaaatg gacagtatcg
     4141 ggggcatcat ccatgtggac cagatagtgc agctggccaa tcagctgttc ctgcaggacc
     4201 aggtgtcatt tggaaccaca gacatcctgg aaaaagacca agccacagga atttgccacc
     4261 tcttttatga cagtgctcct agtggggctt atggtacaat gacctaccta acaaaagcgg
     4321 tcgcttctca tttgcaggaa ctgctgccca gttcaggctg ccagatgcag tagggcctta
     4381 cccataaaca tgtccaccct ctgaatctgt catttgtaga ctcattggga acgttcttct
     4441 gccttccccc tcgagcccct gttctgtcac aaaaagcatg tcccaccaga aagcttcgca
     4501 gggagagatg acagcactca ggctgaaagc aggatgtgtt taagccacag ctaatgctga
     4561 tgaacccgct gctcccctga gaagcaccct gggatcattt tctaggttca tttcactgga
     4621 acatttgtca cagcatctgc tctcatggac tccaaaaagg aactagaaat tggtctcttc
     4681 tgagtgcaga gtgaacagag aagcagctta aattggctct caccaagagt tacagaaata
     4741 ggttcgatga actagtgaca catcttgaag gtgaaaagat ccttccagca acaatagcta
     4801 ggcaggcaga ccctgacagg ggcacatctt aacagaatga cgtctgtctg aaccaagctg
     4861 ttcaccaagc agaataagtc tgtctttctg gaagtttttt cagtgcccta gtctactctg
     4921 gtcatgtaat tcttcattgc caaaatggac taaatttgcc ctagttaact ttgacttata
     4981 ctcttc
//
