LOCUS       NM_008832               6115 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript
            variant 1, mRNA.
ACCESSION   NM_008832
VERSION     NM_008832.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Bowman,C.E., Zhao,L., Hartung,T. and Wolfgang,M.J.
  TITLE     Requirement for the Mitochondrial Pyruvate Carrier in Mammalian
            Development Revealed by a Hypomorphic Allelic Series
  JOURNAL   Mol Cell Biol 36 (15), 2089-2104 (2016)
   PUBMED   27215380
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6115)
  AUTHORS   Kalsotra,A., Xiao,X., Ward,A.J., Castle,J.C., Johnson,J.M.,
            Burge,C.B. and Cooper,T.A.
  TITLE     A postnatal switch of CELF and MBNL proteins reprograms alternative
            splicing in the developing heart
  JOURNAL   Proc Natl Acad Sci U S A 105 (51), 20333-20338 (2008)
   PUBMED   19075228
REFERENCE   4  (bases 1 to 6115)
  AUTHORS   Davidson,J.J., Ozcelik,T., Hamacher,C., Willems,P.J., Francke,U.
            and Kilimann,M.W.
  TITLE     cDNA cloning of a liver isoform of the phosphorylase kinase alpha
            subunit and mapping of the gene to Xp22.2-p22.1, the region of
            human X-linked liver glycogenosis
  JOURNAL   Proc Natl Acad Sci U S A 89 (6), 2096-2100 (1992)
   PUBMED   1372435
REFERENCE   5  (bases 1 to 6115)
  AUTHORS   Hamvas,R.M., Zinn,A., Keer,J.T., Fisher,E.M., Beer-Romero,P.,
            Brown,S.D. and Page,D.C.
  TITLE     Rps4 maps near the inactivation center on the mouse X chromosome
  JOURNAL   Genomics 12 (2), 363-367 (1992)
   PUBMED   1740345
REFERENCE   6  (bases 1 to 6115)
  AUTHORS   Brockdorff,N., Kay,G., Smith,S., Keer,J.T., Hamvas,R.M., Brown,S.D.
            and Rastan,S.
  TITLE     High-density molecular map of the central span of the mouse X
            chromosome
  JOURNAL   Genomics 10 (1), 17-22 (1991)
   PUBMED   1675191
REFERENCE   7  (bases 1 to 6115)
  AUTHORS   Varsanyi,M., Groschel-Stewart,U. and Heilmeyer,M.G. Jr.
  TITLE     Characterization of a Ca2+ -dependent protein kinase in skeletal
            muscle membranes of I-strain and wild-type mice
  JOURNAL   Eur J Biochem 87 (2), 331-340 (1978)
   PUBMED   668698
REFERENCE   8  (bases 1 to 6115)
  AUTHORS   Gross,S.R., Longshore,M.A. and Pangburn,S.
  TITLE     The phosphorylase kinase deficiency (Phk) locus in the mouse:
            evidence that the mutant allele codes for an enzyme with an
            abnormal structure
  JOURNAL   Biochem Genet 13 (9-10), 567-584 (1975)
   PUBMED   1203056
REFERENCE   9  (bases 1 to 6115)
  AUTHORS   Huijing,F.
  TITLE     Phosphorylase kinase deficiency in mice
  JOURNAL   FEBS Lett 10 (5), 328-332 (1970)
   PUBMED   11945425
REFERENCE   10 (bases 1 to 6115)
  AUTHORS   LYON,J.B. Jr. and PORTER,J.
  TITLE     The relation of phosphorylase to glycogenolysis in skeletal muscle
            and heart of mice
  JOURNAL   J Biol Chem 238, 1-11 (1963)
   PUBMED   13931951
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732405.8 and AL805925.6.
            
            On Jul 26, 2007 this sequence version replaced NM_008832.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X74616.1, SRR10223969.19987.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-400               AL732405.8         57292-57691         c
            401-559             AL732405.8         55666-55824         c
            560-607             AL732405.8         52526-52573         c
            608-776             AL732405.8         49988-50156         c
            777-859             AL732405.8         39033-39115         c
            860-940             AL732405.8         34783-34863         c
            941-1039            AL732405.8         30762-30860         c
            1040-1186           AL732405.8         29356-29502         c
            1187-1240           AL732405.8         23597-23650         c
            1241-1363           AL732405.8         22487-22609         c
            1364-1459           AL732405.8         11631-11726         c
            1460-1567           AL732405.8         10847-10954         c
            1568-1646           AL732405.8         6072-6150           c
            1647-1781           AL805925.6         101766-101900       c
            1782-1891           AL805925.6         90654-90763         c
            1892-2036           AL805925.6         89157-89301         c
            2037-2115           AL805925.6         84979-85057         c
            2116-2285           AL805925.6         75254-75423         c
            2286-2462           AL805925.6         72785-72961         c
            2463-2554           AL805925.6         71215-71306         c
            2555-2694           AL805925.6         70573-70712         c
            2695-2851           AL805925.6         60997-61153         c
            2852-2931           AL805925.6         60363-60442         c
            2932-3010           AL805925.6         57061-57139         c
            3011-3140           AL805925.6         56801-56930         c
            3141-3242           AL805925.6         52873-52974         c
            3243-3358           AL805925.6         48092-48207         c
            3359-3397           AL805925.6         47766-47804         c
            3398-3448           AL805925.6         43756-43806         c
            3449-3619           AL805925.6         36443-36613         c
            3620-3673           AL805925.6         33132-33185         c
            3674-3874           AL805925.6         32466-32666         c
            3875-6115           AL805925.6         29664-31904         c
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 45.47 cM"
     gene            1..6115
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /note="phosphorylase kinase alpha 1"
                     /db_xref="GeneID:18679"
                     /db_xref="MGI:MGI:97576"
     exon            1..400
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     CDS             323..4048
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /EC_number="2.7.11.19"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phosphorylase kinase alpha M subunit"
                     /codon_start=1
                     /product="phosphorylase b kinase regulatory subunit alpha,
                     skeletal muscle isoform isoform 1"
                     /protein_id="NP_032858.2"
                     /db_xref="CCDS:CCDS30323.1"
                     /db_xref="GeneID:18679"
                     /db_xref="MGI:MGI:97576"
                     /translation="MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWV
                     RDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESF
                     KYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLD
                     EVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLF
                     GVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLV
                     ELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTY
                     FILDGIFSGNVEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDR
                     VPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEE
                     IKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTS
                     KLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPI
                     SHTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPE
                     GKLYSEDYDEDYEDDLDSGNWMDSYDSTSNARCGDEVARYLDRLLAHTVPHPKLAPTS
                     RKGGLDRFRAAVQTTCDLMSLVAKAKELHIQNVHMYLPTKLFQPSRPSLNLLDSPESP
                     QDSQVPSVHVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYSMKGPDWNTE
                     LYEEGGATVRELLSELYVKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSYQKHLT
                     VGLPPEPREKTISAPLPYEALTKLIDEASEGDMSISTLTQEIMVYLAMYMRTQPGLFA
                     EMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVER
                     SVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSVSMESQTSGGH
                     PSGVDLMSPSFLSPAACIAASSGSFPTVCDHQTSKDSRQGQWQRRRRLDGALNRVPIG
                     FYQKVWKILQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLV
                     EAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICT
                     LLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ"
     exon            401..559
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            560..607
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            608..776
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            777..859
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            860..940
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1039
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1186
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1240
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1363
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1459
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1567
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1646
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1781
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1891
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            1892..2036
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2115
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2285
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            2286..2462
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            2463..2554
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            2555..2694
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            2695..2851
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            2852..2931
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            2932..3010
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            3011..3140
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            3141..3242
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            3243..3358
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            3359..3397
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            3398..3448
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            3449..3619
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3673
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            3674..3874
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     exon            3875..6115
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4708..4713
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /note="hexamer: ATTAAA"
     polyA_site      4726
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /note="major polyA site"
     regulatory      6095..6100
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
                     /note="hexamer: AATAAA"
     polyA_site      6114
                     /gene="Phka1"
                     /gene_synonym="5330411D17; 9830108K24Rik; Phka"
ORIGIN      
        1 aagggaggcg gcacccgggg cgctgacccg ctgctgtcgc cggccttcag tctcagggcc
       61 gccccggtct ccttcagtca agcggaggat gccggtgcgc cacccgggga ccacgggaga
      121 ctgagtggag acggggtctt tgccggccga agcctagacc gcgggaggct gagccgcggc
      181 ctgaagtgga ctctgaggcc gccggaacca ggtgacgcgc gggaaccatt ggccacacga
      241 gcaagggagc gtttagaagg tttccgtagg cctggcgtcc gagcttcccg tgtgtgcagt
      301 gggggattag ggacgtggca ccatgaggag ccgcagtaac tcgggagtcc gcctggacgg
      361 ctatgctcgg ctggtgcatc agaccatcct gtgccatcag aatccagtga caggcttgct
      421 tccagccagc tatgatcaaa aagatgcctg ggtccgagat aatgtgtaca gtatcctggc
      481 tgtgtggggt ttgggcctgg catatcgcaa aaatgctgac cgtgatgagg acaaggcaaa
      541 ggcctatgaa ctggagcaga gtgtagtgaa gttaatgagg ggactgctgc actgcatgat
      601 cagacaggtg gataaagtag agtccttcaa gtacagtcag agtactaaag atagcctcca
      661 tgccaagtac aacaccaaaa cttgtgccac tgtggtgggt gatgaccagt ggggacacct
      721 gcagttggat gctacttctg tgtacctgct cttcctagca caaatgactg cctcaggact
      781 ccatatcatc cacagcctag acgaagtcaa ttttatacag aacctcgtgt tttacattga
      841 agctgcatat aaaactgctg actttgggat atgggaacgt ggcgataaga caaaccaagg
      901 catctcggaa ttgaatgcga gttcagttgg aatggccaag gcagccctgg aagcactaga
      961 tgaattagac ttgtttggtg tgaaaggtgg gccacaatca gttatccatg tcctggctga
     1021 tgaagtccaa cactgccagt ctatcctgaa ttcactactg cccagggctt caacatccaa
     1081 agaagttgat gccagtctgc tctcagtggt ctctttccca gcctttgctg tagaggacag
     1141 ccatttggtg gagctcacca aacaggagat catcaccaag cttcagggtc gttatggttg
     1201 ctgtcgtttt ctgcgagatg gatataaaac tcctaaagag gatcccaatc gcctatacta
     1261 tgaaccagct gagctgaagc tatttgaaaa cattgagtgc gaatggccat tgttctggac
     1321 atactttatc cttgatggga tcttcagtgg caacgtagaa caggttcaag aatatagaga
     1381 ggctcttgat gcagtcctca tcaagggcaa aaatggagtc cctcttcttc cagagctgta
     1441 cagtgtccct cctgacaggg ttgatgaaga gtatcaaaat ccccacactg tggatcgagt
     1501 ccctatggga aaattgcctc acatgtgggg tcagtctcta tacattttag gaagcttgat
     1561 ggcagaggga tttttagctc ctggagaaat tgatcccctg aatcgtaggt tttctactgt
     1621 gccaaagcca gatgtggtgg ttcaagtctc cattctggct gaaacagaag aaatcaaggc
     1681 cattttgaag gacaaaggaa ttgatgtgga gaccattgct gaagtgtacc ccataagagt
     1741 acagccagct cgtattctca gccatattta ttctagtcta ggatgcaaca gtagaatgaa
     1801 actcagtgga cgaccctaca ggctcatggg tgtgcttgga acatcaaaac tttatgacat
     1861 tcgcaaaact atctttactt tcactccaca gtttatagac cagcaacagt tctacctggc
     1921 tctggacaac cagatgatag tagaaatgct cagaacagac ctttcctacc tctgtagccg
     1981 ctggaggatg acaggccagc ccacgatcac tttccctatc tcgcacacca tgcttgatga
     2041 agatggaacc agcttgaatt caagtatctt ggcagcactc cgaaaaatgc aggatggcta
     2101 ttttggtggg gccaggatcc aaacaggtaa attgtcagag tttttgacaa catcttgctg
     2161 cacacactta agcttcatgg accctggacc tgagggtaag ctgtacagtg aagattatga
     2221 tgaagactat gaagatgatt tggactctgg caactggatg gacagctatg attcaacaag
     2281 taatgctcgc tgtggtgatg aagttgcccg ttatttagac cgccttttgg cacacactgt
     2341 tccccatcct aaactagctc ctacctcacg gaagggaggg ctagatcggt tccgagctgc
     2401 tgtgcaaaca acttgcgact taatgtcctt ggtggccaag gccaaggagc tgcatataca
     2461 gaatgttcac atgtatcttc ctacaaagtt atttcagcct tctcgtcctt cactcaactt
     2521 acttgactcc cctgagtctc cacaagatag ccaggttcct tccgttcatg tagaagtgca
     2581 tcttcctagg gaccagtctg gggaagtgga cttccagtca ttggtttcac agttgaagga
     2641 gacctcaagc ttacaggagc aagctgatat actctacatg ctgtattcaa tgaaaggacc
     2701 tgactggaac actgaattgt atgaagaagg gggggctact gtcagagagc ttcttagtga
     2761 actgtatgtc aaagttggtg aaattcggca ctggggtctg atccgatata tctctgggat
     2821 cttacggaag aaagtggagg cacttgatga ggcctgcaca gaccttctgt cctaccagaa
     2881 acacctgaca gtaggacttc ctccagaacc tcgagagaag accatctctg cgcctctacc
     2941 gtacgaggca ctcactaagc tgatagatga agccagtgaa ggcgacatga gcatctcaac
     3001 ccttacacag gaaataatgg tctatcttgc catgtatatg agaactcagc ctggcctctt
     3061 tgcagaaatg ttcagacttc gaatcggttt gatcatacaa gttatggcaa cagaactagc
     3121 acactctctt cgatgttcag ctgaggaagc cacagagggc ctgatgaatc tcagtccttc
     3181 agccatgaag aacctcctgc atcacattct cagtggcaaa gagtttggag tggaacgaag
     3241 cgttcgtccc actgattcaa atgtgagtcc tgctatttcc atccatgaga ttggtgctgt
     3301 tggagcaaca aaaactgaac gaactggaat catgcagtta aaaagtgaga taaagcaggt
     3361 ggaatttcgt aggctgtctg tctcgatgga gagtcagact agtggtggtc atccctcggg
     3421 tgtagatttg atgtcgccat cctttctgtc ccctgcagcc tgtattgctg caagtagtgg
     3481 atcctttcct acggtgtgtg atcatcagac atctaaagat agtcgtcaag gccagtggca
     3541 acgcaggaga aggctagatg gagcactaaa tagagtacca attggatttt atcaaaaagt
     3601 atggaaaatt ttgcagaaat gtcatgggct ttctgtggaa ggttttgttc ttccctcttc
     3661 aaccactagg gagatgaccc caggtgagat taaattctct gtccatgtgg agtctgtcct
     3721 gaatcgtgtc cctcagccag aataccgcca acttctggtt gaagccatcc ttgttctcac
     3781 catgctggca gatattgaaa ttcatagcat tgggagcatc attgctgtgg agaaaatagt
     3841 tcatattgcc aacgacttgt tccttcaaga acagaaaacc ctcggcgcag atgataccat
     3901 gttggcaaag gatcctgcat ctggcatctg tactctcctg tatgacagtg cacccagtgg
     3961 cagatttggc accatgacct acctctccaa ggcagctgcc acctacgtgc aggagtttct
     4021 gccacacagc ctctgtgcca tgcagtgagg gctgtgggtt ctgcctgtta gaacacttca
     4081 gtagctagcc cgtctggttg attgtgcatg gaactgacat gttaacacct gatcactccc
     4141 ccaggccctt ttcctgcccc atccctccta ggaagagaga actttgctga cgatctcact
     4201 acttttgaag aaaagaagcc ctatctggag acttgactcc agagcgaagc cctatctgga
     4261 gacttgactc cagagcgaag gttcaactgc gcctgcagtc actatcccta cttttgtggt
     4321 tcatagaagt ctgcatgtgc gttgattctg tagagctgtt agtggcttag tttttaaact
     4381 cctattgggg agtaggtatt aataacttat tcctgtaagt tggttttctt atttgttggt
     4441 ttttacgtgt gtatatgagt ggtgggtatt gattaggcca tcatttcaag agagtagatg
     4501 ctaagttgga tttcgtgctg ctgtaaattg gaactgtcga ctctaagtgc ctttacttag
     4561 gaaaagccag taccacatca tggcttcctt cttcactcaa aggctgcttt gctctgagtg
     4621 gggaaagagt gggtgttttg tagaagcatt gcctttcatt gttctttcta tatctgtcca
     4681 caatgcctac tgtctctgtg gtgaaatatt aaaatatgta agctcacatg gaagtttaag
     4741 aatttctgtg ttaatacttg gaataaccca gagaaaagaa gccagaagcc taattccaga
     4801 gaggaaggga aagaagttct gtaggtagtg ctttacacat agggggccga gagaacatca
     4861 gaggcaggta ggttgcttgt ggacagcctt agacaccaaa tccttgcctc tggagtgtag
     4921 ccacacacaa cacagagagt aactgaagcc tatcttaagc aaagagaaac cacccgtcca
     4981 ctctggggct gccctactga atgccccttt ccaggacctt ggtattcctt ctcattcagt
     5041 ccccagcaca tccggctacc cctagctctc tccacattca ttcctcaagt ctctcattta
     5101 catggtttga ctttttaatt ctagtactta caacctttaa tcttggtaac tgtggccaaa
     5161 tatctattgt tatacatatc tctgaatgtg cttgtaagtg gttaaataat ttgtaagcat
     5221 ttttgatctg tctgtctgtc tctacatgga tggactgaat aagaatgtaa ataatattta
     5281 ttgcaaagca agcctggtca tacttgctgc ttgagaagct gaggcaggag gatcatgaat
     5341 tcaaagccag cctgggatac atagcaagtt tcagggcagt gtgggtatga ggttgtgtct
     5401 caaaaacatt tgaaagcatg ttgtgcatgt gtgagagagt ggcgtggctg gtctcaggct
     5461 gacactcgaa gggtagtaga aatatccctt ctggcctatt gtctgcttcg ctgtgtaggt
     5521 agcctgactt gacagaggtt tctcctggtt ttccttgttt ctacccagag aatttcctac
     5581 tcccctttcc ctccaaatgt taagggtttt ctcccagaac agtaatgggg ctaaaggggc
     5641 agtgagctcc aaacagtcac agagtgagga gtcttctgag ctccctggct aagtgcagcg
     5701 tgctagcaga caggcagggg atgcaaacgg ctgccgggcc acctctcccc tctcacaaac
     5761 catataaaca cagggagggg ctttctcacc acttctgagg agactgtaca gcccgttttg
     5821 tgcctgtgtg tgtgttcatg gcctgtaagt gtaccccaag cactgtgggc gggagctacc
     5881 acttctttga gaacctctgc tttacggttg acactgatgc ttatctttct ctggagttac
     5941 ggatttgcct gtggatttaa tgctcacacc atgctattta atttttaaag taacttttca
     6001 gaaaatatta ctggcgtttt gttcatttta agactgttaa catgaagaaa agtgtgctag
     6061 aactttttaa aaacacataa tgtcttgaag ttagaataaa cactaagaaa tgact
//
