LOCUS       NM_145604               2874 bp    mRNA    linear   ROD 06-AUG-2024
DEFINITION  Mus musculus phagosome assembly factor 1 (Phaf1), transcript
            variant 2, mRNA.
ACCESSION   NM_145604
VERSION     NM_145604.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Franco-Romero,A., Morbidoni,V., Milan,G., Sartori,R., Wulff,J.,
            Romanello,V., Armani,A., Salviati,L., Conte,M., Salvioli,S.,
            Franceschi,C., Buonomo,V., Swoboda,C.O., Grumati,P., Pannone,L.,
            Martinelli,S., Jefferies,H.B., Dikic,I., van der Laan,J.,
            Cabreiro,F., Millay,D.P., Tooze,S.A., Trevisson,E. and Sandri,M.
  TITLE     C16ORF70/MYTHO promotes healthy aging in C.elegans and prevents
            cellular senescence in mammals
  JOURNAL   J Clin Invest 134 (15), e165814 (2024)
   PUBMED   38869949
  REMARK    GeneRIF: C16ORF70/MYTHO promotes healthy aging in C.elegans and
            prevents cellular senescence in mammals.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Leduc-Gaudet,J.P., Franco-Romero,A., Cefis,M., Moamer,A.,
            Broering,F.E., Milan,G., Sartori,R., Chaffer,T.J., Dulac,M.,
            Marcangeli,V., Mayaki,D., Huck,L., Shams,A., Morais,J.A.,
            Duchesne,E., Lochmuller,H., Sandri,M., Hussain,S.N.A. and
            Gouspillou,G.
  TITLE     MYTHO is a novel regulator of skeletal muscle autophagy and
            integrity
  JOURNAL   Nat Commun 14 (1), 1199 (2023)
   PUBMED   36864049
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2874)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   4  (bases 1 to 2874)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2874)
  AUTHORS   Keegan,C.E., Hutz,J.E., Else,T., Adamska,M., Shah,S.P., Kent,A.E.,
            Howes,J.M., Beamer,W.G. and Hammer,G.D.
  TITLE     Urogenital and caudal dysgenesis in adrenocortical dysplasia (acd)
            mice is caused by a splicing mutation in a novel telomeric
            regulator
  JOURNAL   Hum Mol Genet 14 (1), 113-123 (2005)
   PUBMED   15537664
REFERENCE   6  (bases 1 to 2874)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   7  (bases 1 to 2874)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124713.5.
            
            On Mar 20, 2020 this sequence version replaced NM_145604.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057919.1, AK156120.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-300               AC124713.5         151288-151587
            301-383             AC124713.5         157229-157311
            384-467             AC124713.5         160583-160666
            468-511             AC124713.5         164524-164567
            512-591             AC124713.5         165772-165851
            592-686             AC124713.5         166079-166173
            687-784             AC124713.5         166942-167039
            785-897             AC124713.5         167128-167240
            898-1031            AC124713.5         172522-172655
            1032-1115           AC124713.5         172939-173022
            1116-1198           AC124713.5         174197-174279
            1199-1242           AC124713.5         174844-174887
            1243-1286           AC124713.5         175520-175563
            1287-1345           AC124713.5         175698-175756
            1346-1418           AC124713.5         176820-176892
            1419-2874           AC124713.5         177699-179154
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 53.04 cM"
     gene            1..2874
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /note="phagosome assembly factor 1"
                     /db_xref="GeneID:234678"
                     /db_xref="MGI:MGI:2443049"
     exon            1..300
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     CDS             237..1505
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /note="isoform 2 is encoded by transcript variant 2;
                     lin-10 homolog; UPF0183 protein C16orf70 homolog"
                     /codon_start=1
                     /product="phagosome assembly factor 1 isoform 2"
                     /protein_id="NP_663579.2"
                     /db_xref="CCDS:CCDS22593.1"
                     /db_xref="GeneID:234678"
                     /db_xref="MGI:MGI:2443049"
                     /translation="MLDLEVVPERSLGNEQWEFTLGMPLAQAVAILQKHCRIIRNVQV
                     LYSEQSPLSHDLILNLTQDGITLLFDAFNQRLKVIEVCELTKVKLKYCGVHFNSQAIA
                     PTIEQIDQSFGATHPGVYNSTEQLFHLNFRGLSFSFQLDSWTEAPKYEPNFAHGLASL
                     QIPHGATVKRMYIYSGNSLQDTKAPVMPLSCFLGNVYAESVDVLRDGTGPSGLRLRLL
                     AAGCGPGVLADAKMRVFERAVYFGDSCQDVLSMLGSPHKVFYKSEDKMKIHSPSPHKQ
                     VPSKCNDYFFNYFTLGVDILFDANTHKVKKFVLHTNYPGHYNFNIYHRCEFKIPLAIK
                     KENAGGQTEICTTYSKWDSIQELLGHPVEKPVVLHRSSSPNNTNPFGSTFCFGLQRMI
                     FEVMQNNHIASVTLYGPPRPGAHLRTAELP"
     exon            301..383
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            384..467
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            468..511
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            512..591
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            592..686
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            687..784
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            785..897
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            898..1031
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1115
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1198
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1242
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1286
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1345
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1418
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..2874
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2874
                     /gene="Phaf1"
                     /gene_synonym="D230025D16Rik; Lin10; Mytho"
                     /note="major polyA site"
ORIGIN      
        1 agttgctctg ggcgctgctg ttgctacagc ctctcctgcc ggggctgctg cccgggaggt
       61 tgaagggagg tgaagtgtgt tgttgtgttc gtgctgccgt cgccgcccgg aaccgagtgg
      121 agagccggac gtccgcgggc tggcgagagc gaccagtcag cagctgcgtg ctctcctcag
      181 gccgggtcct gtctgcgctg ccgcagggag gccgcccggc ccagcgaacc gacccaatgc
      241 tggacctgga ggtggtgcct gaacgctctc tggggaacga gcaatgggaa ttcactctgg
      301 gaatgcctct ggcgcaggcg gtcgccattc ttcagaagca ctgtcgcatc atcagaaacg
      361 tccaggttct ctacagtgag cagtctcctc taagccatga cctcatcctt aacctgactc
      421 aggacgggat cacactactg tttgatgctt tcaaccagag actcaaggtg attgaagttt
      481 gtgagttgac aaaagtaaag ttaaagtatt gtggagtgca ttttaactct caggccatcg
      541 ctcctaccat tgagcaaatt gaccagtctt ttggcgcaac ccatcctgga gtgtacaact
      601 ctactgagca gctcttccac ctcaacttcc gaggactgtc tttctctttt cagttagact
      661 cgtggacgga ggctcccaag tatgagccca attttgccca tggtctagct tctcttcaga
      721 taccccatgg agcaactgta aaacgaatgt acatctatag tgggaacagc ctccaggata
      781 ccaaggctcc tgtgatgccc ttgagctgtt tcctggggaa tgtatatgct gaaagtgtag
      841 acgttcttcg agatggaact ggaccctcag ggttgcgact tcgcttgctc gctgcaggtt
      901 gtggacctgg agtgttagca gatgccaaaa tgagagtatt tgagcgtgcg gtgtattttg
      961 gtgattcttg ccaagatgtt cttagcatgc ttggctctcc acacaaagtc ttctataaat
     1021 ctgaagacaa gatgaaaatt cactctcctt cccctcataa acaagttccg tccaagtgca
     1081 acgactactt ttttaactat tttactctcg gagtggacat cctgtttgat gcaaatacac
     1141 acaaagtgaa gaagtttgtc ctgcatacca attaccctgg gcattataat tttaacattt
     1201 accaccgctg tgagttcaag atcccactgg ccataaagaa agaaaatgca ggtggtcaga
     1261 ctgaaatatg cacaacctac agcaagtggg acagcatcca ggagcttctg ggccatcctg
     1321 tggagaagcc tgttgtcttg cacaggtcct catccccaaa taacaccaat ccgtttggct
     1381 ccacattctg ctttggtctt cagcggatga tctttgaggt catgcagaac aaccacattg
     1441 cctcagtgac cctgtatggc ccccccaggc ctggtgccca cctgagaaca gcagagctgc
     1501 cctaaggaca tcactgtttt ccctgtccat ggaactgtgc gtcacatcca gctcagtggg
     1561 tctctgtgcc accctgtgtg tttgcttgga caccttggca gtgttgaagg gccctgggac
     1621 tcagaagggt ttggaagtag gatttgggct gagtcctctg ccgtgggacg gaggacccag
     1681 atactctgtc ctaagcctgc tgttgccagg agagaacaca gtgtgcaaaa agaagcacaa
     1741 acttagagcc atgatgaggg ctctcataac taacatgtaa taaaggacac gtcagcccct
     1801 gccctgctca catagggaag acaggactct ttctctagct aagaaaatat tcccaggatg
     1861 agaacaggct cctgcttttg gcccgacatt gccacgtggc ccctaaggtg agcagagcag
     1921 gtagcatgtc ggtagtactt ggttgcaata tttgcactcc atccacttta gtgacttgat
     1981 gagctggctg tggctggtgt ggcccactgc ccttccctgc ttctcctgtg agtgctcctt
     2041 gccgtgccca ggtggcacct aagtggatga aatcgtttcc taccaggtat gtaccaggcc
     2101 tggttcccac tgctgctctg ggagccagcc tttaaggtca cctgtgtctc ttggtccaac
     2161 ctggctgaca gggctgcatg tctccatctt gtttgcctct tttatttaat gccaaagtgt
     2221 tagccaaaga caccttcctc cttttgttac attacagcat tctgttatgc actgtgggcc
     2281 agctccttgc cctagttctg ggcactggcc tggcctggcc tggctacttg gtttgaggcc
     2341 tagaggctca aggaacacta ggctgcttgg tttcttacat gggtcttgct aatgggaagt
     2401 agcatatatg tgctttaaaa tagtaatcct tttgaaaaga attgagaaga gaaacatata
     2461 attttatccc atttttaata ttttggtcta gcaacttgtg atacatagat gacaattttg
     2521 tgagtttttc aaatgtgtgt acagattttt gtaaataatg acttttgtaa ttaactcatg
     2581 tacagcctca tcttgtatag ttcatgatga atgtgcaggg gacctgcccc agctgtctgt
     2641 atggttcctg ggcctacagc caggcctgtg tggcactccc atgactgatt tgacaggtgt
     2701 cttcccattt ggaccttcgt ctggccagag accctgcaca tcccactgtc agggtagcca
     2761 ggactcttcc catcctcgtg atcagaggag acttcctcgt cccccttcca ataaagcacc
     2821 tatgcccaca gtgacagcgt gccatgtgct gccactgggc tgtttgtact aaaa
//
